1
vote
2
replies
39
views
0
votes
0
replies
20
views
3
votes
8
replies
6.4k
views
7 follow
updated 2 hours ago by
Sd
•
0
•
written 5.1 years ago by
Nicolas Rosewick
11k
0
votes
2
replies
53
views
0
votes
0
replies
35
views
2
votes
3
replies
91
views
0
votes
1
reply
48
views
updated 2 hours ago by
Mensur Dlakic
★
27k
•
written 5 hours ago by
Arun Sai Kumar
•
0
0
votes
0
replies
48
views
0
votes
1
reply
173
views
1
vote
1
reply
91
views
updated 5 hours ago by
Ram
43k
•
written 12 hours ago by
marco.barr
▴
90
0
votes
0
replies
48
views
0
votes
1
reply
91
views
0
votes
0
replies
56
views
0
votes
0
replies
58
views
17 hours ago by
James
▴
10
0
votes
1
reply
548
views
1
vote
2
replies
143
views
updated 13 hours ago by
ATpoint
82k
•
written 19 hours ago by
poordumbsillyidiot
•
0
0
votes
1
reply
162
views
0
votes
0
replies
64
views
0
votes
0
replies
91
views
0
votes
1
reply
110
views
updated 1 day ago by
Ram
43k
•
written 1 day ago by
ernestine.kubi
•
0
0
votes
0
replies
78
views
1
vote
2
replies
215
views
15 hours ago by
rianna.collins
•
0
0
votes
1
reply
150
views
0
votes
0
replies
116
views
0
votes
1
reply
423
views
updated 1 day ago by
Ram
43k
•
written 1 day ago by
Dr Huma Naz
•
0
0
votes
5
replies
321
views
1
vote
1
reply
212
views
updated 1 day ago by
Ram
43k
•
written 2 days ago by
Jonathan Yoou
▴
60
1
vote
2
replies
270
views
1
vote
3
replies
253
views
0
votes
1
reply
166
views
updated 1 day ago by
Adam
▴
30
•
written 2 days ago by
Ahiad Chen Zion
•
0
0
votes
0
replies
120
views
0
votes
0
replies
116
views
updated 2 days ago by
Ram
43k
•
written 2 days ago by
Alexandros
•
0
0
votes
1
reply
157
views
0
votes
3
replies
261
views
0
votes
0
replies
123
views
0
votes
0
replies
123
views
1
vote
2
replies
212
views
0
votes
0
replies
105
views
0
votes
0
replies
109
views
0
votes
0
replies
123
views
2 days ago by
Jacob
•
0
0
votes
1
reply
173
views
0
votes
3
replies
264
views
3
votes
7
replies
306
views
0
votes
0
replies
120
views
3 days ago by
Lluís R.
★
1.2k
0
votes
0
replies
120
views
0
votes
4
replies
232
views
0
votes
0
replies
214
views
0
votes
3
replies
203
views
0
votes
0
replies
90
views
0
votes
2
replies
190
views
3 days ago by
Bosberg
▴
50
Recent Votes
Recent Locations •
All
Switzerland, just now
France, 4 minutes ago
France/Nantes/Institut du Thorax - INSERM UMR1087, 5 minutes ago
Canada, 8 minutes ago
France, 8 minutes ago
Germany, 9 minutes ago
Canada, 9 minutes ago
Recent Awards •
All
Popular Question to
yvonnehhe
•
0
Popular Question to
curious
▴
750
Scholar to
jared.andrews07
★
16k
Popular Question to
hafiz.talhamalik
▴
350
Popular Question to
Hayler Edu
▴
40
Popular Question to
eesiribloom
▴
80
Popular Question to
g.persic1991
•
0
Recent Replies
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
If you know what the boundaries of your construct look like then trim the left-end of the read using the tag on that end (`ktrim=l`). Then …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> Not every read in your data is going to match a guide.
That is also true for sure. Let me clarify what I mean. For every read you should…
Answer: VG : No reference-sense paths available in the graph; falling back to generic pa
by
anovak
▴
120
If your GFA has paths in it that are P lines with names that don't include a sample name, contig name, and separators, then those are what …
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
> if you look at a fastq file you should be able to tell for every read definitely, if it has a perfect match for a guide in it and what gu…
Answer: DiffBind: no peaks in DBA
by
jared.andrews07
★
16k
Because `dba.analyze` is not meant to be run directly on a peaks file. Have you read the documentation?
In addition, a ChIP-seq experiment…
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as yo…
Comment: Converting CRAM to FastQ
by
GenoMax
142k
Use `samtools view ` for this conversion. See discussion in https://www.biostars.org/p/9592860/
Specifically @jkbonfield's comment here --…
Comment: How to access TCGA samples that were treated with a specific drug?
by
GenoMax
142k
No that was only from Breast cancer. You could try selecting all data and see if you are able to see all treatments in the set.
No idea a…
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as you …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> Can you try lowering the k to k=9?
Wouldn't that mess with the results since all my kmers are 20bp?
> You don't have any duplicate sequ…
Comment: Provean help
by
Mensur Dlakic
★
27k
> Else, I will happy to send over the data and kindly perform the analysis for me.
This website is meant to provide advice, not service. I…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴
40
Thank you! Naive question, but does that list include all treatments in TCGA? I.e. is there anything missing from there, or that's it?
Als…
Comment: Base Quality Score Recalibration with different reference genome
by
dtnondorf
•
0
Interesting! Thank you for such a quick response!
Comment: GATK genomicsDBimport intervals for WGS
by
Sd
•
0
I also have similar question. I sliced the genomic bed file with 50kb windows and 1kb padding into ~700 bed files; each bed file contains 9…
Traffic: 2695 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6