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1,000 results • Page
1 of 20
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
7
views
ERROR: LoadError: UndefVarError: `composition` not defined ?
Protpram
20 minutes ago by
Sapphire
• 0
0
votes
0
replies
18
views
Complex Phylogenetic Tree Annotation
annotation
tree
protein
phylogenetic
1 hour ago by
Aanushka
• 0
1
vote
2
replies
48
views
Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
RNA-evidence
genome
prediction
masking
42 minutes ago by
Ayish
• 0
0
votes
1
reply
68
views
What is the possibility of Depth (DP) being higher than the coverage
GATK
DP
Coverage
Depth
HaplotypeCaller
updated 5 hours ago by
seidel
11k • written 6 hours ago by
dare_devil
★ 3.1k
0
votes
2
replies
137
views
What does "OS_CNSR" and "PFS_CNSR" mean?
survival
cancer
r
2 hours ago by
JACKY
▴ 100
0
votes
1
reply
93
views
CIBERSORT Error: File not found
CIBERSORT
updated 4 hours ago by
ATpoint
72k • written 14 hours ago by
jcara514
• 0
3
votes
3
replies
189
views
retaining only the clusters of interest
seurat
single-cell
scRna-seq
updated 7 hours ago by
rfran010
▴ 160 • written 16 hours ago by
shamza
• 0
0
votes
2
replies
223
views
Diff Bind Questions
DiffBind
CHiP-Seq
updated 17 hours ago by
Ram
39k • written 1 day ago by
B.N.
• 0
0
votes
3
replies
213
views
Gviz Coverage Plots
gviz
scrnaseq
cellranger
r
updated 12 hours ago by
Ram
39k • written 18 hours ago by
Researcher
• 0
1
vote
0
replies
95
views
Alternative approach to rarefying in 16S rRNA analysis
16S
Rarefying
rRNA
21 hours ago by
zhangdengwei
▴ 190
0
votes
1
reply
125
views
phage genome submission in ncbi genebank
phage
table2asn
ncbi
updated 15 hours ago by
acvill
▴ 290 • written 22 hours ago by
tahsinkhan570
• 0
2
votes
3
replies
211
views
Same GEO Accession, different SRR number, how to download this RNA-seq paired-end data?
sra-toolkit
fastq
RNA-seq
SRA
updated 20 hours ago by
GenoMax
129k • written 22 hours ago by
ev97
• 0
0
votes
0
replies
105
views
Which is the minor allele in ped file in plink?
plink
updated 23 hours ago by
GenoMax
129k • written 23 hours ago by
khn
▴ 130
0
votes
2
replies
184
views
Correct way to remove Nextera adapters from ITS sequences
adapters
ITS
trimmomatic
13 hours ago by
mattze731
▴ 10
0
votes
0
replies
99
views
What statistical test should I use to analyse my two set of transcriptomique data?
transcriptome
1 day ago by
F.Bedjou
▴ 10
0
votes
0
replies
82
views
Difference MITOS WebServer and MITOS 2 Webserver
MITOS2
WebServer
Difference
MITOS
1 day ago by
hashim.rana11
▴ 20
0
votes
0
replies
110
views
Integrate RDS files
integration
1 day ago by
Andy
▴ 50
0
votes
1
reply
158
views
Information on sequin RNA spike in standards
Sequin
Spike-in
RNA-seq
updated 3 hours ago by
ATpoint
72k • written 1 day ago by
akainth
▴ 10
1
vote
9
replies
319
views
Trying to edit VCF file
VCF
R
bcftools
snpsaurus
updated 1 day ago by
Ram
39k • written 1 day ago by
peavy
• 0
0
votes
1
reply
381
views
How to quantification proteomics data
proteomics
updated 1 day ago by
eqoa45
• 0 • written 19 months ago by
LeeLee
▴ 10
1
vote
4
replies
224
views
Is hg38 on the multiz 30-way alignment inaccurate?
maf
UCSC
PHAST
multiz
1 day ago by
Daniel
▴ 30
0
votes
2
replies
1.4k
views
How to perform statistical analysis of label free experiment using results from Proteome Discoverer?
Proteome-Discoverer
Label-Free
Proteomics
updated 1 day ago by
Ram
39k • written 4.5 years ago by
k2bhide
▴ 80
0
votes
0
replies
103
views
Label free quantification in Proteome discoverer software
proteomics
quantification
spectrometry
shotgun
1 day ago by
eqoa45
• 0
0
votes
1
reply
168
views
CHiP-Seq Questions
DiffBind
CHiP-Seq
updated 1 day ago by
jared.andrews07
★ 14k • written 1 day ago by
B.N.
• 0
1
vote
0
replies
109
views
How to predict gene amplification from RNAseq and ATACseq data?
amplification
ATAC
1 day ago by
Dan
▴ 120
1
vote
2
replies
175
views
hisat2 Error 137
hisat2
RNA-seq
1 day ago by
Forough
▴ 10
0
votes
0
replies
110
views
Error occured at the PHG imputation step: allele in genotype not in the variant context
PHG
Imputation
PracticalHaplotypeGraph
Haplotype
1 day ago by
xeod21
• 0
2
votes
5
replies
288
views
Embryo transcriptome
database
transcriptome
SRA
updated 1 day ago by
ATpoint
72k • written 1 day ago by
firefox91
• 0
1
vote
3
replies
182
views
Obtain a --gene-trans-map file after rnaSPAdes
rnaSPAdes
Trinity
1 day ago by
UserA
• 0
0
votes
0
replies
86
views
How to extract only neutral markers from low-coverage whole genome sequencing?
low-coverage
whole-genome-sequencing
updated 1 day ago by
Ram
39k • written 1 day ago by
beausoleilmo
▴ 530
0
votes
4
replies
200
views
Difference between USCS exon coordinates and ensembl
exons
Ensembl
1 day ago by
amy__
▴ 50
0
votes
0
replies
87
views
Why sone miRNAs are not present in TCGA data
TCGA
miRNA
RNA-seq
2 days ago by
nicolo.gualandi
• 0
0
votes
0
replies
77
views
Prediction of altrered metabolites from altered gene expression
Gene
Metabolites
2 days ago by
english.server
▴ 290
0
votes
6
replies
328
views
variant allelic fraction
vep
updated 20 hours ago by
Ram
39k • written 2 days ago by
Jom
• 0
0
votes
1
reply
145
views
Pairwise Alignment
Pairwise-Alignment
updated 1 day ago by
Ram
39k • written 2 days ago by
Fatemeh
• 0
0
votes
0
replies
95
views
How to perform a phylogenetic analysis from a vcf file
variants
haplotyping
phylogeny
vcf
2 days ago by
rj.rezwan
• 0
0
votes
1
reply
159
views
Where to get the molecular subtype information of STAD sample in TCGA
TCGA
database
updated 2 days ago by
Hamid Ghaedi
2.9k • written 3 days ago by
younglin113
▴ 50
0
votes
0
replies
136
views
How to keep ComplexHeatmap from reordering annotations
Dendrogram
HeatmapAnnotations
R
Heatmap
ComplexHeatmap
3 days ago by
Manuel
• 0
1
vote
2
replies
191
views
Differential protein expression analysis
R
proteomic
statistics
18 minutes ago by
Ribo
▴ 40
0
votes
3
replies
322
views
(ERR): "index/Trinity.fa" does not exist or is not a Bowtie 2 index Exiting now ...
bowtie2
align_stats
trinity
2 days ago by
eimanpharmacist
▴ 20
0
votes
4
replies
366
views
What are recommended parameters for the local protein alignment ?
protein
alignment
3 days ago by
Alexander
▴ 70
0
votes
0
replies
130
views
microsatellite, SSR, using Galaxy
Galaxy
SSR
using
microsatellite
4 days ago by
m.esmaeilpour
▴ 10
0
votes
0
replies
145
views
decoupleR & Transcription Factory Activity Inference: pvalue or padj?
pvalue
padj
decoupleR
4 days ago by
OST
▴ 10
2
votes
3
replies
340
views
Low mapping percentage
mapping
STAR
RNAseq
3 days ago by
Sib
▴ 40
0
votes
0
replies
172
views
Visualization App for RNASeq Differential Expression and Enrichment Analysis
differential
visualization
RNASeq
analysis
expression
enrichment
4 days ago by
Mark
• 0
0
votes
0
replies
159
views
busco id description
OrthoDB
BUSCO
updated 4 days ago by
Ram
39k • written 4 days ago by
angelina
• 0
0
votes
5
replies
388
views
how to Construct a Newick tree file from five large fasta files
tree
Newick
alignment
updated 4 days ago by
Joe
21k • written 5 days ago by
gunala.nikhil
• 0
0
votes
0
replies
164
views
NMDS plotting issue
NMDS
metagenomic
4 days ago by
arshad1292
▴ 90
0
votes
2
replies
238
views
featurecounts not working on mirbase annotation file
usegalaxy
miRNA-seq
1 day ago by
demoraesdiogo2017
▴ 90
0
votes
0
replies
142
views
IGV insertions
insertions
IGV
4 days ago by
christoph.schubart
• 0
1,000 results • Page
1 of 20
Recent Votes
A: Extract Reads From A Bam File That Fall Within A Given Region
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
Comment: Alignment of case vs. control from different origin
A: understanding bedtools coverage output
Answer: Alignment of case vs. control from different origin
Comment: Alignment of case vs. control from different origin
Comment: Gene prediction software
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Recent Replies
Comment: Differential protein expression analysis
by
Ribo
▴ 40
Thank you! Is there a recommended tutorial for proteomics analysis?
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
I clustered the samples into subtypes and am now comparing the differential expression across the subtypes.
Answer: Limma returned only positive logFC values
by
Gordon Smyth
★ 6.1k
No, your code isn't correct. You are testing an intercept equal to zero instead of testing for a logFC. I wonder what comparison you were t…
Answer: Extract sequences from a fastq file by a list of IDs
by
GenoMax
129k
Using `filterbyname.sh` from [**BBMap suite**][1]: You need to include the `/1` in the header in your list file (here I am using the `nam…
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
Yeah, I added the design matrix. `design <- model.matrix(~0+group)`. These are raw counts.
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Ayish
• 0
Thank you for reply. I have Illumina paired-end reads. Would it be fine if I use hard-masking for STAR and soft-masked genome for BRAKER2? …
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Darked89
4.4k
Soft masking the genome (unless something changed) not make any difference for STAR. Depending on how your RNA-Seq is done (Illumina? paire…
Answer: How to handle NaN in emmax Kinship matrix?
by
Thu
• 0
Hi! Thanks for sharing your solution! It works! :)
Comment: Gene prediction software
by
Darked89
4.4k
Looks like there are five Clarias genomes: * https://www.ncbi.nlm.nih.gov/genome/?term=txid13012[Organism:exp] The most complete seems t…
Comment: Alignment of case vs. control from different origin
by
sativus
▴ 10
Again, thank you so much for these very clearly explained summaries. After reading some articles on the matter, i feel i have a much better…
Answer: Extract sequences from a fastq file by a list of IDs
by
colindaven
4.9k
Some people I know have used filter-fastq successfully: https://github.com/Floor-Lab/filter-fastq
Comment: Extract sequences from a fastq file by a list of IDs
by
Pierre Lindenbaum
154k
> but none of them work for me https://meta.stackexchange.com/questions/147616/
Comment: Extract sequences from a fastq file by a list of IDs
by
Pierre Lindenbaum
154k
https://meta.stackexchange.com/questions/147616/
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
• 0
I have tried this one before but the output file is empty
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
• 0
I have tried all of them but none of them work for me
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