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1,000 results • Page
4 of 20
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
193
views
SeuratDisk: including "counts" dataset in conversion to AnnData
Seurat
SeuratDisk
AnnData
updated 14 days ago by
Ram
43k • written 15 days ago by
roussine
▴ 10
0
votes
0
replies
182
views
I am getting more annotation hits from HOMER than I have actual peaks. Is that normal?
ATAC-seq
HOMER
15 days ago by
Ronin
• 0
4
votes
13
replies
869
views
RNAseq of primary tumor and metastases in two different organ
metastases
RNA-seq
DEG
R
updated 10 days ago by
BioinfGuru
★ 1.7k • written 16 days ago by
matteo.levorato
• 0
2
votes
1
reply
250
views
VEP annotation --per_gene allele choice
VEP
updated 14 days ago by
Ram
43k • written 15 days ago by
atariw
▴ 10
0
votes
2
replies
284
views
Are GeneExpression Subtype annotations removed from TCGA-GBM?
tcga-gbm
subtyping
tcga
updated 14 days ago by
Zhenyu Zhang
★ 1.2k • written 15 days ago by
Apollonia
• 0
1
vote
1
reply
247
views
RNA seq normalization and gene gene correlation
rna-seq
pearson-correlation
vst
updated 15 days ago by
Ram
43k • written 15 days ago by
Fish
• 0
0
votes
0
replies
184
views
Snakemake fails to find conda in PBS
snakemake
15 days ago by
yixinzeng
• 0
0
votes
0
replies
189
views
KaryoPlot: Gene coverage over centromeric regions
Gene
coverage
Centromere
Bedtools
15 days ago by
Mary
• 0
0
votes
0
replies
182
views
Protein stability analysis
mutation
analysis
15 days ago by
marco.barr
▴ 80
1
vote
4
replies
392
views
what is the normal range of htseq-count output for homo sapiens RNA-Seq
Transcriptomics
htseq-count
RNA-Seq
NGS
hisat2
updated 14 days ago by
BioinfGuru
★ 1.7k • written 15 days ago by
Morteza
• 0
3
votes
1
reply
264
views
how to change the gene header names in a fasta file?
fasta
bioawk
awk
seqkit
updated 15 days ago by
Ram
43k • written 15 days ago by
rj.rezwan
• 0
0
votes
0
replies
171
views
Multiple Spike-Ins
MeRIP
Spike-In
15 days ago by
Adam
▴ 30
0
votes
0
replies
161
views
Tools for Sequence Consensus
Consensus-Sequence
bacteria
updated 15 days ago by
Ram
43k • written 15 days ago by
Reno
• 0
2
votes
2
replies
275
views
DepMap: siRNA and CRSIPR discrepancy
DepMap
updated 15 days ago by
ATpoint
82k • written 16 days ago by
Shicheng Guo
★ 9.4k
1
vote
0
replies
158
views
phasing tumor long-reads
nanopore
ONT
haplotag
phasing
tumor
16 days ago by
eesiribloom
▴ 80
0
votes
3
replies
322
views
10x GEX and ADT data analysis in Seurat
Seurat
scRNA-seq
ADT
Cite-seq
GEX
updated 14 days ago by
ATpoint
82k • written 16 days ago by
gdfsnkfns
• 0
0
votes
4
replies
317
views
Questions about transfering wgs file to reference genome file
fasta
cram
genome
16 days ago by
me
• 0
0
votes
2
replies
267
views
WGCNA
PCA
WGCNA
EIGENGENE
15 days ago by
rajasekargutha
▴ 60
0
votes
3
replies
264
views
BLASTP web- <20aa peptides- interpreting results/ minimum E value?
blast
peptide
blastp
alignment
updated 15 days ago by
GenoMax
141k • written 16 days ago by
neish
• 0
1
vote
0
replies
156
views
DESeq2 comparing timepoints within treatment and account for background control change
RNAseq
DEG
DESeq2
updated 16 days ago by
Ram
43k • written 16 days ago by
ian.will
▴ 30
0
votes
0
replies
107
views
Surrogate variable analysis for paired RNA seq experiment
sva
rnaseq
ruvseq
16 days ago by
nhaus
▴ 310
1
vote
3
replies
280
views
True variants selection
vcf
bcftools
updated 15 days ago by
dthorbur
★ 1.9k • written 16 days ago by
maevalefeuvre
• 0
0
votes
1
reply
165
views
How to reveal real AF of variant if duplicates can't be removed (such as in amplicon-seq)
allele-frequency
amplicon-seq
updated 16 days ago by
Ram
43k • written 16 days ago by
CY
▴ 750
0
votes
0
replies
109
views
Creating a BSgenome data package from a NCBI assembly - Virus
Virus
BSgenomeForge
BSgenome
R
16 days ago by
JirMan
▴ 20
1
vote
1
reply
175
views
Failed to download data from EBI with ascp
EBI
aspera
updated 16 days ago by
GenoMax
141k • written 16 days ago by
biock
▴ 60
0
votes
0
replies
399
views
BETA whether on the same scale when performing rank-based inverse normal transformation on the same type of phenotype data for two cohorts separately…
normal
inverse
transformation
16 days ago by
Isaac
• 0
0
votes
0
replies
108
views
Combining VG graphs
vg
graphs
combine
16 days ago by
AshleeThomson
▴ 80
0
votes
0
replies
114
views
Installing SALSA and HIRise scafolding software
scafolding
SALSA
genome-assembly
HIRise
updated 16 days ago by
Ram
43k • written 16 days ago by
Lissa Cruz Saavedra
• 0
0
votes
1
reply
186
views
Extract Di-nucleotide/Tri-Nucleotide substitution frequencies from MSA/VCF file
MSA
substitution
VCFstats
Evolution
VCF
updated 16 days ago by
Pierre Lindenbaum
161k • written 16 days ago by
Saran
▴ 50
0
votes
2
replies
274
views
Abundance Calculation in MetaPhlAn 3: Methodology, TPM or RPKM, and the Treatment of Unclassified Reads
MetaPhlAn
Normalization
RPKM
TPM
14 days ago by
ramin.k2013
• 0
0
votes
0
replies
162
views
Normalization in Metagenomics Sequencing By Total Number of Reads: Pre- or Post-Filtering and the Role of Unclassified Reads
Normalization
Metagenomics
updated 16 days ago by
Ram
43k • written 16 days ago by
ramin.k2013
• 0
0
votes
1
reply
173
views
Help with generating annotation database for dog genome to use in ANNOVAR for variant annotation of dog tumor sequences
ANNOVAR
updated 16 days ago by
Pierre Lindenbaum
161k • written 16 days ago by
sainavyav22
• 0
0
votes
9
replies
589
views
99.9999% of Q30 bases is normal?
RNA-seq
fastp
updated 15 days ago by
LauferVA
4.2k • written 17 days ago by
Aki
▴ 10
0
votes
0
replies
97
views
Issue while using circle_dat function from GOplot in R
GOplot
R
17 days ago by
ckelly96
• 0
0
votes
2
replies
196
views
Can't figure out plink --sample-diff
plink
17 days ago by
curious
▴ 750
0
votes
1
reply
154
views
Geneious Prime (Consense Sequence)
Consense
Sequence
updated 17 days ago by
GenoMax
141k • written 17 days ago by
Reno
• 0
1
vote
2
replies
221
views
convert a seurat object to expressionset
Seurat
16 days ago by
Bine
▴ 60
2
votes
4
replies
401
views
Per Base Sequence Content - continuous raise of G%
fastqc
updated 17 days ago by
GenoMax
141k • written 18 days ago by
pl.terzian
• 0
0
votes
0
replies
121
views
Inferring cell identity/genotype in single cells with missing information
cell
seurat
single
score
17 days ago by
txema.heredia
▴ 110
0
votes
0
replies
103
views
cibersortx colnames
gema122
17 days ago by
gem1
• 0
0
votes
2
replies
222
views
How to detect and visualize ORFs in bacterial genome?
statistics
visualization
ORFs
genome
15 days ago by
marongiu.luigi
▴ 710
0
votes
8
replies
5.3k
views
8 follow
cellranger mkref failed
next-gen-sequencing
software-error
RNA-seq
updated 17 days ago by
EricYang
• 0 • written 4.7 years ago by
Dan
▴ 180
0
votes
0
replies
103
views
Confused about the results of a function in dada2 pipeline sample inference step
Microbiome
R
dada2
17 days ago by
Mohamed Samir
▴ 20
1
vote
4
replies
815
views
I want to calculate if the sample has whole genome duplication event or not?
sequencing
wgs
17 days ago by
Hyper_Odin
▴ 310
2
votes
4
replies
296
views
Addmetadata to seurat obj
seurat
17 days ago by
synat.keam
▴ 100
0
votes
0
replies
102
views
Methylation array analysis using dmpFinder
epic
methylation
normalization
dmp
mset
17 days ago by
Roy
▴ 10
0
votes
0
replies
192
views
Confused by the `--ld-window` flag in Vcftools. What does the number of SNPs between SNPs mean?
tabix
vcftools
17 days ago by
rijan_dhakal2055
• 0
0
votes
5
replies
403
views
Used featureCounts to generate a count matrix for sc RNA-seq and it has all 0s.
scRNA-seq
updated 17 days ago by
GenoMax
141k • written 18 days ago by
Shay
• 0
0
votes
0
replies
105
views
Databases with GoF (Gain of Function) annotations.
gof
lof
gain-of-function
18 days ago by
_quantum_girl_
▴ 10
0
votes
2
replies
248
views
Problem with downloading genome in SnpEff
SnpEff
17 days ago by
Javier
• 0
1,000 results • Page
4 of 20
Recent Votes
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i
How do I find out the read lenght of a fastq file?
Calculate Allele balance by sample
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
Answer: Is there a tool that sorts gtf files?
Answer: gvcf joint calling
Answer: Heatmap and rna-seq
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rohitsatyam102
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candron
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35k
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Recent Replies
Comment: gvcf joint calling
by
Jeremy Leipzig
22k
can you show us an exonic position in your VCF file that is all `./.`?
Comment: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
by
O.rka
▴ 710
Thank you. This answers my question. The reason I am asking is because I’m trying to do set enrichment analysis with BRENDA pathways using…
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i
by
colindaven
6.4k
I used to use nextflow in a conda env. That requires the env to be active when a pipeline is started. Also I have productive pipelines whic…
Comment: different FeatureCounts output for the same data
by
Istvan Albert
100k
just to clarify, it is not that the program algorithm works differently but the meaning of the flags changed; before -p was sufficient to…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★ 1.7k
Thanks Ram. Using merge maxed out my ram on a large list, but your suggestion directed me to purrr::reduce which works great.
Comment: ScRNA data question
by
starswillfade
▴ 10
features <- SelectIntegrationFeatures(object.list = merged_dat) data.anchors <- FindIntegrationAnchors(object.list = merged_dat, …
Comment: gvcf joint calling
by
zihanss
• 0
Thank you for your help! I really appreciate it! And you know, the merged WES gVCF files still have "NA" loci. For such cases, I am quite …
Comment: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Guille
• 0
Thank you for your answer! The MDS plot seems to group most treatment groups close-by, so I'm starting to suspect the effect of treatment …
Answer: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
by
Mensur Dlakic
★ 27k
They say that we remember better things we figured out on our own, rather than things we were taught. You were literally two clicks away fr…
Comment: Heatmap and rna-seq
by
dsull
★ 5.8k
https://maayanlab.cloud/clustergrammer/ It's a web-based tool (can also be a python jupyter widget) that I find useful for exploring a pat…
Answer: Limma Analysis Agilent Microarray Data (GPL1708)
by
Gordon Smyth
★ 7.0k
There is an Agilent array case study in the limma User's Guide, which you might find helpful and somewhat simpler. I don't recommend col…
Answer: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Gordon Smyth
★ 7.0k
The comparison you've done seems exactly right to test whether treatment 2 modifies or enhances the effect of treatment 1. Have you exam…
Answer: How to use limma to find differentially expressed genes in response to a continu
by
Gordon Smyth
★ 7.0k
There's no need for form a contrast because the score variable is already in the model. A continuous variable is its own contrast. Just run…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Oh wow, bedops closest-features, how did I overlook that!! I didn't know about it; thank you! Your awk script is beautiful. This worked per…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Thank you Pierre! I'll try this out with my full data set.
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