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481 results • Page
3 of 10
Sort: Views
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Views
Votes
Replies
2
votes
5
replies
722
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 11 days ago by
Jesse
▴ 740 • written 12 days ago by
Ann
★ 2.4k
2
votes
5
replies
684
views
samtools mpileup taking too long
Bam
samtools
sam
alignment
updated 27 days ago by
Ram
43k • written 7 weeks ago by
K
• 0
1
vote
0
replies
678
views
Conditions for valid batch effect correction
r
batch-effect
updated 15 days ago by
Ram
43k • written 5.2 years ago by
Sebastian Hesse
▴ 340
1
vote
1
reply
662
views
Save experiment with batch effects - Protein microarray
statistics
protein-array
batch-effect
updated 15 days ago by
Ram
43k • written 4.4 years ago by
s.lima.diogenes
▴ 10
0
votes
1
reply
659
views
How to find the co ordinates of long reads (simulated by Badreads) with respect to the reference genome
long-reads
reference-genome
assembly
updated 27 days ago by
Ram
43k • written 2.7 years ago by
Ashi
▴ 20
2
votes
2
replies
635
views
Batch correction for DE analysis
batch-correction
mRNA
RNA-seq
DEGs
MDSplot
updated 15 days ago by
Ram
43k • written 2.6 years ago by
Yoonji
▴ 10
0
votes
6
replies
619
views
Best practices for differential expression analysis with low-yield Nanopore/ONT direct cDNA data?
differential-expression
RNA-Seq
ONT
Nanopore
updated 23 days ago by
Ram
43k • written 5 months ago by
tw_140
• 0
0
votes
4
replies
618
views
Per base sequence content failed miserably
fastqc
sequence
trimmomatic
NGS
assembly
13 days ago by
Kai Xin
• 0
0
votes
9
replies
613
views
99.9999% of Q30 bases is normal?
RNA-seq
fastp
updated 20 days ago by
LauferVA
4.2k • written 22 days ago by
Aki
▴ 20
1
vote
1
reply
600
views
accessing the global gut microbial gene catalogue (MEDUSA)
medusa
gene-catalogue
gut-microbiome
updated 21 days ago by
Ram
43k • written 3.3 years ago by
ramin.k2013
• 0
0
votes
0
replies
597
views
Avoid genome circularization with Canu assembler
circularization
ONT
Canu
repetitive-regions
assembler
updated 27 days ago by
Ram
43k • written 2.9 years ago by
Maria
• 0
4
votes
9
replies
590
views
variant calling
variant-calling
updated 29 days ago by
Ram
43k • written 4 weeks ago by
dalibenam64
• 0
4
votes
7
replies
582
views
DESeq2 Multifactor Design
RNA-seq
DESeq2
updated 18 days ago by
Ram
43k • written 19 days ago by
AHerik
▴ 20
0
votes
6
replies
581
views
PacBio adapters in transcriptome assembly from short read data?
adapter
transcriptome
sequencing
pacbio
vecscreen
17 days ago by
Dunois
★ 2.5k
0
votes
2
replies
581
views
Bacterial contamination in human DNA sample
Long-read-sequencing
Bacterial-contamination
updated 27 days ago by
Ram
43k • written 22 months ago by
priya.bmg
▴ 60
0
votes
2
replies
568
views
How to get the reference panel for UKBB
UKBB
GWAS
clump
13 days ago by
航太郎
• 0
0
votes
5
replies
566
views
seg fault, core dumped with manta 1.6.0
manta
gdb
updated 23 days ago by
Ram
43k • written 27 days ago by
Greg
• 0
0
votes
0
replies
565
views
Correlation between cell type prediction scores and individual gene expression in spatial transcriptomic datasets
single-cell
Spatial-Transcriptomics
5 days ago by
biocellbio
• 0
0
votes
11
replies
563
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
6 days ago by
atowns21
• 0
0
votes
2
replies
558
views
autodockvina
python
python3
autodock
updated 13 days ago by
Ram
43k • written 4 months ago by
iamsmor
• 0
0
votes
1
reply
554
views
Differential Expression Analysis for miRNA
limma
Differential-gene-Expression
miRNA
updated 23 days ago by
Ram
43k • written 3.0 years ago by
Al
• 0
1
vote
0
replies
546
views
The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
Primers
TRINITY
12 days ago by
mathavanbioinfo
▴ 90
0
votes
4
replies
543
views
How to reduce the variation of CIBERSORTx results?
CIBERSORTx
deconcolution
14 days ago by
feather-W
• 0
0
votes
1
reply
543
views
Basic stats in hierfstat
Fst
stats
hierfstat
updated 15 hours ago by
mdav
• 0 • written 18 months ago by
Zoe
• 0
2
votes
5
replies
517
views
Where to find the homopolymer regions bed file for Hg002 genome?
simple-repeats
hg002
bed
homopolymer-regions
15 days ago by
Bikram Kumar
• 0
0
votes
5
replies
509
views
How to assure that sequence consensus is genuine?
genome
26 days ago by
marongiu.luigi
▴ 710
3
votes
3
replies
500
views
Free/open source 23andme-like analysis
SNPs
genomics
NGS
VCF
updated 12 days ago by
swbarnes2
14k • written 13 days ago by
noodle
▴ 580
0
votes
1
reply
492
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 7 days ago by
ginellegrenier
• 0 • written 4 months ago by
Clayton
• 0
0
votes
6
replies
482
views
Error in running Mauve alignner in Ubuntu 20.04
java
linux
aligner
mauve
22 days ago by
marongiu.luigi
▴ 710
0
votes
0
replies
480
views
How to map Uniref90 genes (IDs) to Antibiotic resistant genes databases?
CARD
metagenomics
Humann2
antibiotic-resistant-genes
uniref
updated 21 days ago by
Ram
43k • written 2.1 years ago by
ramin.k2013
• 0
0
votes
10
replies
479
views
Low mapping rate with Salmon
RNA-seq
Salmon
Quantification
updated 2 days ago by
i.sudbery
19k • written 10 days ago by
Patadu94
• 0
2
votes
5
replies
479
views
Marking duplicates using UMIs
Deduplication
UMI
updated 10 days ago by
i.sudbery
19k • written 11 days ago by
Lipika
• 0
1
vote
1
reply
475
views
PLINK multivariable GLM output: p-value without BETA and SE
linear-model
glm
plink
updated 24 days ago by
chrchang523
10k • written 24 days ago by
dsbusiness135
▴ 10
1
vote
1
reply
470
views
Load a full GFF3 into annotation track using arrow (Apollo)
arrow
python-apollo
Apollo
13 days ago by
renan.igor
• 0
0
votes
6
replies
460
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 8 days ago by
gv
• 0 • written 4 weeks ago by
Srinka
▴ 20
0
votes
0
replies
450
views
Tools for identifying trans-splicing from long read data
long-read
splicing
nanopore
trans-splicing
updated 27 days ago by
Ram
43k • written 14 months ago by
dthorbur
★ 1.9k
0
votes
2
replies
443
views
QC exclusion of A/T or G/C alleles to avoid strand issues
quality-control
SNV
QC
SNP
13 days ago by
_quantum_girl_
▴ 10
0
votes
2
replies
441
views
miRNAs quantification using mirdeep2 tool
differential-expression
mirdeep2
miRNA
13 days ago by
Ashok
• 0
1
vote
8
replies
437
views
Downsampling fastq file
downsample
fastq
2 days ago by
marco.barr
▴ 90
1
vote
1
reply
434
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
updated 11 days ago by
Gordon Smyth
★ 7.1k • written 14 days ago by
Chris
▴ 260
2
votes
6
replies
434
views
create a gene count matrix csv file from seurat object
Seurat
sparseMatrix
23 days ago by
jkim
▴ 170
1
vote
1
reply
418
views
Long reads Annotation
long-reads
pacbio
updated 27 days ago by
Ram
43k • written 2.1 years ago by
Gerardo
• 0
0
votes
7
replies
418
views
DESeq: too low p-value for RNAseq
R
DESeq
p-value
RNA-seq
FDR
updated 27 days ago by
Papyrus
★ 2.9k • written 28 days ago by
doramora
▴ 10
2
votes
4
replies
415
views
Per Base Sequence Content - continuous raise of G%
fastqc
updated 22 days ago by
GenoMax
142k • written 23 days ago by
pl.terzian
• 0
0
votes
0
replies
415
views
Pairwise alignment of two long chromosomes (~100Mbp) with lastz
lastz
alignment
updated 27 days ago by
Ram
43k • written 21 months ago by
vahe.galstyan
• 0
1
vote
4
replies
414
views
what is the normal range of htseq-count output for homo sapiens RNA-Seq
Transcriptomics
htseq-count
RNA-Seq
NGS
hisat2
updated 19 days ago by
BioinfGuru
★ 1.7k • written 20 days ago by
Morteza
• 0
0
votes
5
replies
412
views
Used featureCounts to generate a count matrix for sc RNA-seq and it has all 0s.
scRNA-seq
updated 22 days ago by
GenoMax
142k • written 23 days ago by
Shay
• 0
0
votes
1
reply
410
views
How to add rowLinks, rowTree in SummarizedExperiment manually.
phylogenetic-tree
microbiome
updated 14 days ago by
Ram
43k • written 19 months ago by
Muhammad
• 0
1
vote
3
replies
409
views
multiple sequence alignment
BLAST
multiple-sequence-alignment
clustal-omega
MSA
updated 24 days ago by
Ram
43k • written 26 days ago by
catherinemaria2728
• 0
0
votes
0
replies
406
views
BETA whether on the same scale when performing rank-based inverse normal transformation on the same type of phenotype data for two cohorts separately…
normal
inverse
transformation
21 days ago by
Isaac
• 0
481 results • Page
3 of 10
Recent Votes
Comment: When should I NOT apply batch correction for my single-cell RNAseq data?
Comment: Bacterial plasmid analysis
Answer: Problem with data downloaded from Short Reads Archive (SRA)
Answer: Super ehancers
Comment: Bacterial plasmid analysis
Answer: Why employ normalization methods, and how can they be utilized in DEG analysis?
Answer: Why employ normalization methods, and how can they be utilized in DEG analysis?
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Recent Replies
Answer: RNA-seq data for deep learning classification
by
dsull
★ 5.9k
Honestly speaking, if it's deep learning, it probably doesn't matter that much if you use something like TPMs (probably not raw counts, unl…
Comment: How does gene length effect the number of reads mapped
by
Chen
• 0
Thanks for the detailed answer and I really appreciate it! I still have one question about why the number of reads can be influenced by the…
Comment: Split Fastq Files Into Chunks Of 1M Reads
by
thomas.heigl.ibk
• 0
i tried it. it splits, however, when i compare the first 8 lines of the second split file with the according lines from the original file, …
Comment: DiffBind: no peaks in DBA
by
Ram
43k
> I've also included the txt version of my .csv file No, you added a screenshot of plain text content, which is counterproductive. You can…
Comment: How to access TCGA samples that were treated with a specific drug?
by
GenoMax
142k
At the GDC portal you have to turn the "therapeutic agents" toggle under "Clinical Data Analysis" on. ![drug][1] For Breast cancer you c…
Answer: Loss of 'var' using concatenation of AnnData objects
by
Hugo
• 0
Similar question has been asked and answered on [scverse discourse](https://discourse.scverse.org/t/loosing-anndata-var-layer-when-using-sc…
Comment: Bacterial plasmid analysis
by
Zamin Iqbal
▴ 20
Hi Nicole. 1. The clustering will be quite different between pling and the rep types (because plasmids with one rep type can still be ve…
Comment: Do I need to go back and filter my long-reads?
by
Ram
43k
Do not delete posts that have received feedback. Engage with the user providing you feedback.
Answer: Download eQTL data of one specific gene for all tissues from GTEx
by
bk11
★ 2.4k
Please kindly check your result here. You can click `csv` and download the date in this [link][1]. **OR** If you prefer to use R, there i…
Comment: RNAseq one control two conditions, shared and exclusive genes
by
matteo.levorato
• 0
but why not a single matrix with the 3 different condition and then by lfcShrink function get the DEGs of each condition?
Answer: Super ehancers
by
jared.andrews07
★ 16k
Sure, you can run it on them. Stitching tends to happen less frequently since the ATAC peaks are generally less wide than H3K27ac, but it'l…
Answer: What purposes can TPM values be used for?
by
jared.andrews07
★ 16k
TPMs cannot be used for intersample comparisons, but they can be used for intrasample comparisons (e.g. comparing relative expression level…
Comment: Bacterial plasmid analysis
by
nicole.kavanagh
• 0
Thank you so much for the recommendation and the very clear explanation , it's much appreciated. I have already made core-gene phylogenies/…
Comment: What purposes can TPM values be used for?
by
Adam
▴ 30
Have you run FASTQC and checked the mapping efficiency of WT3? > would running DESeq using TPM values help solve my problem? No, DESeq sh…
Answer: How goes gene length effect the number of reads mapped
by
i.sudbery
19k
See my answer about the what hows and whens of RNA-seq normalisation here: https://www.biostars.org/p/9586553/#9586566
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