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80 results • Page
1 of 2
Sort: Views
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Views
Votes
Replies
0
votes
9
replies
2.5k
views
6 follow
Cannot process all the reads in a fast5 file?
metagenome
base-calling
fastq
nanopore
updated 5 hours ago by
Ram
43k • written 8 months ago by
Gio
• 0
1
vote
3
replies
2.5k
views
Problem to remove subset of patients with plink
plink
PED
updated 6 days ago by
chrchang523
10k • written 6.1 years ago by
Ginevra
▴ 10
2
votes
9
replies
850
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 5 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
0
votes
11
replies
525
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
3 days ago by
atowns21
• 0
0
votes
0
replies
481
views
Correlation between cell type prediction scores and individual gene expression in spatial transcriptomic datasets
single-cell
Spatial-Transcriptomics
2 days ago by
biocellbio
• 0
0
votes
1
reply
477
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 3 days ago by
ginellegrenier
• 0 • written 4 months ago by
Clayton
• 0
0
votes
6
replies
444
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 5 days ago by
gv
• 0 • written 27 days ago by
Srinka
▴ 20
0
votes
8
replies
338
views
Error in cnetplot enrichplot package
R
updated 6 days ago by
Ram
43k • written 6 days ago by
Farhad
• 0
0
votes
7
replies
338
views
Low mapping rate with Salmon
RNA-seq
Salmon
Quantification
updated 2 days ago by
GenoMax
141k • written 6 days ago by
Patadu94
• 0
0
votes
5
replies
335
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 3 days ago by
GenoMax
141k • written 4 days ago by
Bertalan_Takacs
▴ 90
1
vote
3
replies
331
views
PCA plot
DESeq2
PCAplot
updated 16 hours ago by
LauferVA
4.2k • written 4 days ago by
Aaliya
▴ 10
0
votes
5
replies
331
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
updated 1 day ago by
Istvan Albert
100k • written 5 days ago by
sehriban.buyukkilic
▴ 10
0
votes
5
replies
322
views
Should I remove unlocalized scaffols in reference genome before alignment?
qualimap
alignment
minimap2
GRCh38
assembly
4 days ago by
njornet
▴ 20
1
vote
6
replies
305
views
Downsampling fastq file
downsample
fastq
2 hours ago by
marco.barr
▴ 80
0
votes
0
replies
304
views
roary not working with pgap output gff files with fasta sequence but works with prokka's gff outputs
pangenome
PGAP
NCBI
roary
Prokka
4 days ago by
pramach1
▴ 40
2
votes
4
replies
290
views
interpretartion of a vcf file
calling
genotype
ONT
variant
diploid
5 days ago by
samuelkalandarov2002
▴ 10
0
votes
3
replies
278
views
Highest variable features in single cell data
single-cell
updated 2 days ago by
bk11
★ 2.4k • written 4 days ago by
Kazo
• 0
0
votes
0
replies
276
views
Choice of relatedness cutoff for GWAS of a large cohort (>100K samples)
relatedness
IBD
GWAS
updated 6 days ago by
Ram
43k • written 14 months ago by
Oak
▴ 10
1
vote
3
replies
269
views
some error in building kraken2 database
metagenome
kraken2
5 days ago by
Art1ess
• 0
0
votes
2
replies
251
views
How to calculate identity percentage between proteins contained in a FASTA file?
protein
FASTA
alignment
updated 4 days ago by
Ram
43k • written 5 days ago by
v.berriosfarias
▴ 140
0
votes
2
replies
247
views
Annovar using R package
Annovar
gnomAD
R
3 days ago by
DKA
▴ 40
1
vote
3
replies
235
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 17 hours ago by
ATpoint
82k • written 1 day ago by
Francesco
▴ 10
1
vote
3
replies
232
views
Filter BAM to keep only alignments at an interval, ignoring reads spliced over
samtools
bam
splicing
6 days ago by
WouterDeCoster
47k
1
vote
2
replies
224
views
alignment result
RNA-seq
samtools
hisat2
4 days ago by
ahmad.sajad4541
• 0
0
votes
1
reply
207
views
Normalize scRNAseq data to housekeeping genes to compare several datasets
RNA-sequencing
housekeeping
Single-cell
normalization
updated 3 days ago by
ATpoint
82k • written 3 days ago by
AaronJaime
• 0
0
votes
0
replies
206
views
traying to make a maf from an already annotated vcf
vcf
vcf2maf
maf
maftools
VEP
6 days ago by
Javier
• 0
0
votes
0
replies
199
views
Meta analysis of two GWAS summary statistics: one study is case-control and the other is quantitative
GWAS
Meta-analysis
updated 6 days ago by
Ram
43k • written 6 days ago by
Oak
▴ 10
0
votes
0
replies
188
views
Phasing VCF Files and Analyzing Reads with Multiple Variants
haplotypes
vcf
phasing
3 days ago by
HarperReed
• 0
1
vote
1
reply
175
views
Failed kmer content
kmer
illumina
ngs
updated 5 days ago by
Ram
43k • written 6 days ago by
Kasturi
• 0
0
votes
0
replies
168
views
How to visualize/predict the final transcript from Delly output?
WGS
DELLY
3 days ago by
simplitia
▴ 130
0
votes
0
replies
164
views
Running Phylogenetic Analysis With NCBI Genome
population-genetics
phylogenetic
updated 2 days ago by
Ram
43k • written 3 days ago by
SineWave
• 0
0
votes
1
reply
165
views
Plot DNA methylation level of DMR on the basis of certain genes
rrbs
methylkit
updated 5 days ago by
GenoMax
141k • written 5 days ago by
Srinka
▴ 20
0
votes
0
replies
164
views
Is there a way to increase the automatic label text size in Cytoscape?
Cytoscape
3 days ago by
avocado123
• 0
0
votes
1
reply
162
views
Find subcluster under a cluster, find differential genes in one cluster between 2 samples??
clustering
subclustering
scRNAseq
updated 5 days ago by
bk11
★ 2.4k • written 5 days ago by
alphaflylizard
• 0
1
vote
0
replies
162
views
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
dnds
kaks
VCF
3 days ago by
rohitsatyam102
▴ 850
0
votes
0
replies
157
views
RNA-seq: full length gene
RNA-seq
updated 2 days ago by
Ram
43k • written 3 days ago by
Nargis
• 0
0
votes
1
reply
157
views
RNA-seq bacteria contamination
RNA-seq
updated 6 days ago by
GenoMax
141k • written 7 days ago by
sh
• 0
0
votes
1
reply
154
views
How to solve this RoseTTAFold colaboratory error?
modeling
Google
Colaboratory
RoseTTAFold
protein
updated 5 days ago by
Mensur Dlakic
★ 27k • written 5 days ago by
benguyarenbeyaz98
• 0
0
votes
0
replies
154
views
Designing single-stable RNA molecules
structure
RNA
3 days ago by
Edna
• 0
0
votes
1
reply
153
views
GEMMA GWAS how to specify factor or numerical for covariates
covariate
GEMMA
GWAS
updated 4 days ago by
Sofia
• 0 • written 4 days ago by
mawigoj318
• 0
0
votes
0
replies
150
views
How to calculate correlation coefficient for chipseq?
chipseq
bigwigsummary
correlation
3 days ago by
Emily
▴ 10
0
votes
1
reply
148
views
Identification of Transgene insertion sites
Transgene
Whole-Genome-Sequencing
transgene-sites
updated 6 days ago by
Ram
43k • written 6 days ago by
Cameron.walker9900
• 0
0
votes
1
reply
148
views
Subset Seurat object from Xenium spatial data
Seurat
Xenium
Spatial
updated 5 days ago by
bk11
★ 2.4k • written 6 days ago by
Susmita Mandal
▴ 110
0
votes
0
replies
148
views
how to read graph_test output of monocle 3
monocle3
3 days ago by
synat.keam
▴ 100
0
votes
0
replies
148
views
Haplotype Phased Assembly Contigs to Chromosome Annotations
Assembly
phased
Haplotype
Annotation
3 days ago by
turcoa1
• 0
1
vote
0
replies
146
views
Simulation of label-free bottom-up proteomics expression dataset
label-free
bottom-up
lc-ms
proteomics
3 days ago by
KABILAN
▴ 50
0
votes
0
replies
146
views
Why not use iBAQ for calculating differential abundance of proteins?
protein
maxquant
4 days ago by
Aspire
▴ 300
0
votes
0
replies
145
views
Can you give me an example of a small globular protein (PDB) that has disordered alpha-carbons?
protein
pdb
alpha-carbon
4 days ago by
4fzcgueyp5
• 0
2
votes
2
replies
139
views
PDB related issue
rcsb
pdb
updated 11 hours ago by
noodle
▴ 570 • written 14 hours ago by
Nafi
• 0
0
votes
0
replies
139
views
Sequence BLAST in plus/minus and plus/plus
blast
CRISPR
strand
sequencing
5 days ago by
salias
• 0
80 results • Page
1 of 2
Recent Votes
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Answer: How many reads for WGS Sequencing?
Comment: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
NGS forensics: how to know if data is fabricated
Answer: ChIP-seq datasets: input samples omitted?
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Thank you very much for the response. Following your recommendation in pursuing option one by selecting highest expressions and subsequent …
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I think it would be pertinent to use DESEq2 to perform two comparisons: the first one between the control group and condition 1, and the se…
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Assuming you have network access and the md5sum is registered with the EBI's reference server, yes - it'll be downloaded and cached locally.
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I followed your advice and it seems that I'm getting results comparable to what I was getting before. Upon checking with `wc -l` on the ori…
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See the section on non full rank design matrices in the DESeq2 manual. In this case your problem is that healthy and 0dpi samples are e…
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19k
Can you clarify what you mean by "100% of reads pass cutadapt, even though 70% of reads contain adapters and get trimmed. " did you set a m…
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I don't follow your argument for distinguishing between genomic contamination and freud. A data set being bad in terms of genomic contamin…
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Thank you for your detail and great explanation. I do need more time to understand these. And there is still something compuzzling me. For …
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Try using this library [GEOparse][1] [1]: https://github.com/guma44/GEOparse
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OK. Problem solved. The problem was from my end. Thank you both for helping !!
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★ 27k
Appreciate the suggestion, but that ship has sailed and reached the other shore. This happened 10+ years ago. To the best of my knowledge n…
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