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1,000 results • Page
3 of 20
Sort: replies
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Views
Votes
Replies
0
votes
9
replies
1.2k
views
Normalization across different single cell RNAseq experimetns
global-scaling
single-cell
scRNA-seq
batch-effect
updated 9 days ago by
Ram
43k • written 3.9 years ago by
jmah
▴ 20
0
votes
9
replies
838
views
Deseq2 error
R
deseq2
6 months ago by
sgadila
• 0
1
vote
9
replies
1.5k
views
blast retrieves "N/A" for taxonomic data
blast
blastn
updated 6 weeks ago by
flavianegrete
• 0 • written 3.4 years ago by
langziv
▴ 50
0
votes
9
replies
764
views
how to use multiple command in one line with using the output of last command to the next
bedtools
linux
bed
4 months ago by
sata72
• 0
3
votes
9
replies
787
views
Understanding this analysis pipeline
reads
updated 10 months ago by
Ram
43k • written 10 months ago by
artemchuki
• 0
2
votes
9
replies
7.6k
views
6 follow
Read MAP and PED files in PLINK
GWAS
PLINK
updated 4 months ago by
GenoMax
141k • written 6.0 years ago by
ft821525
• 0
7
votes
9
replies
1.1k
views
Calculation of TMB on gene level
genomics
updated 7 months ago by
svp
▴ 680 • written 7 months ago by
smrutimayipanda
▴ 20
0
votes
9
replies
1.1k
views
grep multiple lines form file
shell-script
grep
updated 10 months ago by
Ram
43k • written 3.9 years ago by
harry
▴ 30
0
votes
9
replies
1.4k
views
Samtools sam to bam error
samtools
updated 5 months ago by
Andrea
• 0 • written 2.0 years ago by
kb_93
▴ 10
0
votes
9
replies
1.5k
views
Chipseq analysis on repeat genes
galaxy
repeatmasker
ChIP-seq
repeat-elements
ATAC-seq
updated 7 months ago by
rfran010
▴ 900 • written 7 months ago by
pb11
▴ 10
1
vote
9
replies
4.4k
views
Unable to install bioconda and bowtie
bioconda
conda
updated 4 months ago by
rpolicastro
13k • written 19 months ago by
n.anuragsharma
▴ 30
6
votes
9
replies
2.9k
views
How to analyze the scRNA seq Fastq files from NCBI
SRAtool
Cellranger
NCBI
updated 10 months ago by
Ram
43k • written 2.3 years ago by
aimanbarki
▴ 20
3
votes
9
replies
1.7k
views
How to get negative training set if I don't know what constitutes negative?
machine-learning
dna-sequence
blast
updated 5 months ago by
Ram
43k • written 7.6 years ago by
nafizh
• 0
1
vote
9
replies
1.2k
views
Trying to edit VCF file
VCF
R
bcftools
snpsaurus
updated 10 months ago by
Ram
43k • written 10 months ago by
peavy
• 0
3
votes
9
replies
671
views
STARsolo seg fault without readMapNumber flag
STAR
RNA-seq
STARsolo
Velocyto
updated 6 months ago by
Ram
43k • written 6 months ago by
rbronste
▴ 420
1
vote
9
replies
658
views
Htseq-count reads with missing mate encountered
htseq
htseq-count
5 months ago by
Bjorn
• 0
1
vote
9
replies
1.0k
views
gene annotation
gtf
gene-annotation
updated 11 months ago by
Ram
43k • written 11 months ago by
ahmad
• 0
1
vote
9
replies
520
views
miRNA alignment with Bowtie2
bowtie2
5 weeks ago by
Chironex
▴ 40
0
votes
9
replies
5.4k
views
Calculating FPKM and TPM by hand from htseq-count output?
RNA-Seq
rna-seq
htseq
FPKM
TPM
updated 8 months ago by
ATpoint
82k • written 5.0 years ago by
Joel Wallenius
▴ 210
4
votes
9
replies
2.6k
views
Blast+ remote database names
blast
remote
databases
Blastplus
updated 9 months ago by
GenoMax
141k • written 2.3 years ago by
Komalharini
• 0
2
votes
9
replies
817
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 2 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
4
votes
9
replies
1.2k
views
Snakemake issue with wrappers
Python
Snakemake
5 months ago by
Begonia_pavonina
▴ 150
1
vote
9
replies
615
views
Approach for multi species comparative transcriptomics and differential expression analyses
DE
RNA-seq
transcriptomics
updated 3 months ago by
dthorbur
★ 1.9k • written 3 months ago by
fish_enthusiast
• 0
4
votes
9
replies
1.0k
views
bcl2fastq troubleshooting all reads dumped to "Undetermined"
demultiplex
illumina
sequencing
updated 4 months ago by
GenoMax
141k • written 4 months ago by
MaxF
▴ 120
2
votes
9
replies
1.2k
views
Salmon (or other pseudo-mappers) for multi-species RNAseq read filtering
STAR
Salmon
mapper
aligner
RNAseq
4 months ago by
ian.will
▴ 30
0
votes
9
replies
898
views
fragments file generation via Sinto from CellRanger output
scATAC
alignment
scRNA
Sinto
10x
6 months ago by
ntsopoul
▴ 60
0
votes
9
replies
579
views
99.9999% of Q30 bases is normal?
RNA-seq
fastp
updated 14 days ago by
LauferVA
4.2k • written 15 days ago by
Aki
▴ 10
0
votes
9
replies
479
views
Low number of both surviving reads after trimming
trimmomatic
trimming
28 days ago by
Jay
• 0
0
votes
8
replies
1.1k
views
BAM header edit with a list of amplicons
samtools
BAM
updated 10 months ago by
Ram
43k • written 2.3 years ago by
ltalignani
• 0
1
vote
8
replies
3.4k
views
Best way to address different batches of RNA-seq
sequence
RNA-Seq
RPKM
Fold-Change
batch-effect
updated 8 days ago by
Ram
43k • written 7.0 years ago by
tud55122
▴ 20
1
vote
8
replies
3.9k
views
No gene can be mapped
R
GSEA
updated 4 months ago by
jv
★ 1.8k • written 2.3 years ago by
yang.zhizhang
• 0
0
votes
8
replies
304
views
Error in cnetplot enrichplot package
R
updated 3 days ago by
Ram
43k • written 3 days ago by
Farhad
• 0
1
vote
8
replies
969
views
Reads in IGV are mostly in intron regions
Patchseq
RNA-seq
10 months ago by
Kai_Qi
▴ 130
0
votes
8
replies
1.5k
views
Cannot find Output files after applying Markduplicates with picard tools
picard-tools
MarkDuplicates
updated 6 weeks ago by
xiangbinruan
• 0 • written 19 months ago by
Phoebe Magdy
• 0
0
votes
8
replies
643
views
Phobius error: Could not read provided fasta sequence at phobius.pl line 408
Phobius
updated 8 weeks ago by
ido.idobar
▴ 10 • written 3 months ago by
Elizabeth
▴ 30
3
votes
8
replies
2.0k
views
What is the minimum system requement for oxford nanopore read assembly
Assembly
updated 11 months ago by
Ram
43k • written 5.5 years ago by
nagendranp1991
• 0
2
votes
8
replies
1.0k
views
VCF file CSQ flag
vcf
exome-sequencing
updated 10 months ago by
Ram
43k • written 10 months ago by
Payal
▴ 160
0
votes
8
replies
5.3k
views
8 follow
cellranger mkref failed
next-gen-sequencing
software-error
RNA-seq
updated 16 days ago by
EricYang
• 0 • written 4.7 years ago by
Dan
▴ 180
4
votes
8
replies
1.9k
views
Hello, I have differential ATAC-seq from control and diseased cells (H3K27ac mark as chromatin mark) and wondering how to call enhancers using ROSE …
super-enhancers
Enhancers
updated 12 weeks ago by
jared.andrews07
★ 16k • written 3 months ago by
Manhezz
• 0
1
vote
8
replies
2.5k
views
Using NCBIs EDirect to download pubmed abstract
edirect
pubmed
NCBI
updated 10 months ago by
Ram
43k • written 5.8 years ago by
zorn21g
• 0
0
votes
8
replies
1.9k
views
Chromosome Location of Sequence in Fasta File using Biopython
biopython
python
updated 10 months ago by
Ram
43k • written 4.4 years ago by
muhammad.khizerkiet
• 0
5
votes
8
replies
1.0k
views
sc-RNA seq pipeline
scRNA-seq
NGS
RNA-Seq
3 months ago by
subhiksha
▴ 30
1
vote
8
replies
1.5k
views
counts of cite-seq-count vs. cellrenager varies tremendously
citeseq-count
cellranger
cite-seq
scRNA-seq
updated 4 months ago by
ATpoint
82k • written 9 months ago by
Assa Yeroslaviz
★ 1.8k
0
votes
8
replies
4.4k
views
Downloading single cell data from NCBI
ncbi
rna-seq
fastq-dump
single-cell
updated 8 months ago by
Ram
43k • written 5.4 years ago by
V
▴ 380
6
votes
8
replies
1.2k
views
conda: command not found: gatk.
conda
gatk
10 months ago by
bestone
▴ 30
2
votes
8
replies
684
views
TCGA Biospecimens Slides Extraction
tcga
updated 8 weeks ago by
Zhenyu Zhang
★ 1.2k • written 9 weeks ago by
jain72744
▴ 10
5
votes
8
replies
2.4k
views
SV calling using giraffe/vg
vg
Giraffe
calling
structural
variant
updated 9 months ago by
Jordan M Eizenga
▴ 460 • written 15 months ago by
TN
• 0
0
votes
8
replies
1.5k
views
VCF samtools
vcf
samtools
variant-calling
updated 10 months ago by
Ram
43k • written 2.4 years ago by
Lucía
• 0
0
votes
8
replies
9.9k
views
how to find a read by name in a bam file
next-gen-sequencing
updated 10 months ago by
Ram
43k • written 6.8 years ago by
himanimalhotra89
• 0
0
votes
8
replies
523
views
STAR aligner options
STAR
28 days ago by
theophile
• 0
1,000 results • Page
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Recent Votes
cellular niches analysis with spatial transcriptome data in Seurat and Bioconductor
Answer: Seurat merge and batch correction
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
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Comment: Create a new bed file with all pairwise combinations between two other bed files
by
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• 0
Thank you for replying Pierre. I first added a third column to the SNP bed file, so that I would get a range of 1 for each SNP awk 'B…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
> using bcftools Show us what you tried
Answer: gvcf joint calling
by
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22k
> Hi, guys, there is a question about the genomic gVCF file. I wonder > that since gVCF contains the non-var block records, why after merge…
Comment: Missing protein (VEGF-A) in String db
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Thank you for replying here; this helped me figure out why MAPK10 was missing from my results. It was also marked as a pseudogene in the En…
Comment: How to convert plink files to Hapmap Format
by
Sofia
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These are the first lines of the output: (Please is it normal to have NA in the P value column ?) CHR …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
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So I used the barcodes that I created (combos of positions 1-10 and 11-20) and I obtained similar alignment stats as the paper I pulled the…
Comment: How to convert plink files to Hapmap Format
by
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Thank you so much, it actually worked!
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Hi @efc1e545 , First a caveat. the information we most need in order to help guide you to a successful conclusion is not provided in thi…
Answer: Sequence read length shorter than flow cell specification
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The company probably had you share your run with someone who needed the extra bases. So you get the extra bases free. Just use them unle…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
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43k
Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.
Comment: Annovar using R package
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DKA
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Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.
Comment: Treatment VS Control in Single Cell RNAseq analysis
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82k
Open a new question, with details.
Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets
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I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.
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by
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thank you!
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Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…
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