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187 results • Page
4 of 4
Sort: Rank
Rank
Views
Votes
Replies
2
votes
5
replies
487
views
Fetch table from clinvar database according to a list of rsid
python
clinvar
perl
6 days ago by
ashaneev07
▴ 20
0
votes
1
reply
172
views
Downloading full alignments from Pfam
pfam
updated 6 days ago by
GenoMax
142k • written 6 days ago by
bef1
• 0
0
votes
1
reply
753
views
Can I run cellassign on samples independently if there is batch effect present?
RNA-Seq
R
cellassign
batch-effect
updated 6 days ago by
Francesco
▴ 10 • written 3.3 years ago by
gt
▴ 30
3
votes
2
replies
299
views
ChIP-seq datasets: input samples omitted?
ChIP-seq
Normalization
NGS
updated 6 days ago by
i.sudbery
19k • written 8 days ago by
vanbelj
▴ 40
0
votes
0
replies
123
views
adjusting for confounders in LMER in R
confounders
LMER
R
updated 5 days ago by
dariober
14k • written 7 days ago by
rene.j.erhardt
▴ 20
1
vote
3
replies
306
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 6 days ago by
ATpoint
82k • written 7 days ago by
Francesco
▴ 10
1
vote
3
replies
396
views
Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:1.1.1.4, 1.1.1.-, 1.1.1.303)?
ontology
metagenomics
database
enzymes
genomics
updated 6 days ago by
Mensur Dlakic
★ 27k • written 8 days ago by
O.rka
▴ 710
4
votes
2
replies
305
views
How to use limma to find differentially expressed genes in response to a continuous variable
limma
voom
R
5 days ago by
pairedttest
▴ 10
0
votes
3
replies
403
views
why renaming Idents in Seurat object doesn't work?
Seurat
RenameIdents
R
4 days ago by
Assa Yeroslaviz
★ 1.8k
1
vote
7
replies
521
views
ScRNA data question
scRNA
Vlnplot
Samples
updated 5 days ago by
bk11
★ 2.4k • written 9 days ago by
starswillfade
▴ 10
2
votes
5
replies
387
views
Figures are disappeared from html report of SnpEff
HTML
SnpEff
6 days ago by
analyst
▴ 30
1
vote
3
replies
395
views
PCA plot
DESeq2
PCAplot
updated 6 days ago by
LauferVA
4.2k • written 10 days ago by
Aaliya
▴ 10
5
votes
5
replies
565
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 1 day ago by
Mbofire
• 0 • written 10 days ago by
Ming Tommy Tang
★ 3.9k
1
vote
1
reply
171
views
Including plasmid in alignment
Bacteria
Transcriptomics
BOWTIE2
STAR
4 days ago by
heelpPlease
• 0
5
votes
4
replies
371
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
2 days ago by
Estevão
• 0
2
votes
4
replies
290
views
from row count to tpm
tpm
row-count
normalization
updated 4 days ago by
Ram
43k • written 12 days ago by
michelafrancesconi9
▴ 20
1
vote
8
replies
474
views
Downsampling fastq file
downsample
fastq
4 days ago by
marco.barr
▴ 90
0
votes
10
replies
520
views
Low mapping rate with Salmon
RNA-seq
Salmon
Quantification
updated 5 days ago by
i.sudbery
19k • written 12 days ago by
Patadu94
• 0
2
votes
11
replies
913
views
Questions about a bug when transferring cram file to bam file
sequence
samtools
bcftools
updated 6 days ago by
jkbonfield
★ 1.2k • written 17 days ago by
me
• 0
1
vote
6
replies
332
views
Do I need to go back and filter my long-reads?
alignment
nanopore
filtering
QC
ONT
updated 1 day ago by
GenoMax
142k • written 19 days ago by
eebloom
▴ 80
1
vote
0
replies
186
views
News:
Landscape Genomics course in Switzerland
LFMM
Landscape-Genomics
Sambada
R
Local-Adaptation
2 days ago by
carlopecoraro2
★ 2.5k
1
vote
2
replies
277
views
read length in structural variant calling
nanopore
SV
QC
ONT
variant
1 day ago by
eebloom
▴ 80
0
votes
3
replies
337
views
CWl and toil singularity image e.g busybox? Thank you
toil
singularity
updated 5 days ago by
Ram
43k • written 4 months ago by
Fadi
• 0
4
votes
15
replies
1.6k
views
how to test for differential expression in samples where a global increase in gene expression is expected
edgeR
DESeq
Differential-Expression
RNA-Seq
updated 1 day ago by
Gordon Smyth
★ 7.1k • written 4 months ago by
raplayer
▴ 10
1
vote
4
replies
1.1k
views
Filtering qscore on dorado
dorado
filtering
QC
nanopore
Guppy
2 days ago by
eebloom
▴ 80
29
votes
28
replies
34k
views
11 follow
Split Fastq Files Into Chunks Of 1M Reads
split
fastq
updated 2 days ago by
thomas.heigl.ibk
• 0 • written 12.8 years ago by
Bioscientist
★ 1.7k
2
votes
4
replies
2.6k
views
When to use .vcf or .gvcf files from GATK HaplotypeCaller?
indel
gatk
calling
snp
variant
updated 5 days ago by
Medhat
9.7k • written 24 months ago by
Vitor1
▴ 120
2
votes
5
replies
929
views
Retrieval of Active site information programmatically
Catalytic
Python
Active
PDB
site
Site
updated 5 days ago by
Wayne
★ 2.0k • written 2.0 years ago by
arinjoy
• 0
4
votes
9
replies
2.1k
views
6 follow
Same sequencing sample in multiple lanes. How to analyse it?
sequencing
bowtie2
alignment
samtools
ngs
updated 1 day ago by
ST
• 0 • written 2.2 years ago by
Federico
• 0
0
votes
1
reply
542
views
HOMER on AWS
HOMER
updated 2 days ago by
clairechung112
• 0 • written 2.2 years ago by
Bogdan
★ 1.4k
4
votes
9
replies
2.1k
views
Legend and hap files for imputation with 38 build
reference
38build
impute
imputation
5 days ago by
anna
▴ 20
16
votes
12
replies
7.3k
views
10 follow
how to split multi-fasta file into single fasta file named by header
genome
perl
python3
bash
python
updated 2 days ago by
rsieber
▴ 10 • written 3.1 years ago by
Kumar
▴ 120
2
votes
2
replies
1.8k
views
Parametric or non-parametric tests for qPCR significance testing between treatments?
qPCR
statistical test
parametric
non-parametric
updated 1 day ago by
e.r.zakiev
▴ 210 • written 3.8 years ago by
n.anuragsharma
▴ 40
1
vote
14
replies
2.3k
views
Extract gRNA sequence using cutadapt
cutadapt
trimming
crispr
sequencing
updated 2 days ago by
GenoMax
142k • written 4.5 years ago by
Swimming bird
▴ 20
1
vote
6
replies
2.8k
views
Segmentation fault using gemma
gemma
gwas
updated 5 days ago by
dimpleadiwal050896
• 0 • written 4.9 years ago by
ggman
▴ 90
40
votes
10
replies
42k
views
8 follow
Batch effects : ComBat or removebatcheffects (limma package) ?
limma
sva
Combat
batch-effect
updated 5 days ago by
cwwong13
▴ 40 • written 6.7 years ago by
lessismore
★ 1.3k
2
votes
2
replies
2.6k
views
Pindel Segmentation Fault Error
pindel
updated 21 hours ago by
Ruqaiya
• 0 • written 10.2 years ago by
Yuelin
▴ 20
187 results • Page
4 of 4
Recent Votes
Comment: Raw counts using stringtie
Comment: Raw counts using stringtie
Answer: GRCh38.gmap file
Comment: GRCh38.gmap file
Answer: GRCh38.gmap file
Answer: GRCh38.gmap file
Answer: Design matrix Differential expression analysis
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Yoosef
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Zhenyu Zhang
★ 1.2k
Popular Question
to
psm
▴ 130
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Medhat
9.7k
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Recent Replies
Comment: The GDC Legacy Archive is retiring soon.
by
Zhenyu Zhang
★ 1.2k
If you are talking about methylation idat files, they were all migrated from legacy to the data portal. If you are talking about other pl…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Zhenyu Zhang
★ 1.2k
These TCGA samples were taken 10 ~ 20 years ago, and there is no way they have been treated by the modern immunotherapy. Are you sure tha…
Comment: Design matrix Differential expression analysis
by
Gordon Smyth
★ 7.1k
> When is it recommended to use the design <- cbind(Intercept=1,Group=c(-1,-1,-1,1,1,1)) It is not recommended. No such code appears anywh…
Comment: GRCh38.gmap file
by
lorena9132
▴ 10
Hi Mathew, The .txt files provided in the link differ from the .gmap files, but after several attempts, I succeeded in generating the .gma…
Answer: GRCh38.gmap file
by
Mathew
▴ 30
Hi, I have spent some time searching for a GRCH38 map file for you. I have found a certain publication that uses a hg38 genetic map file…
Answer: Association study using SNP data
by
Mathew
▴ 30
Hi, You asked three questions so I will attempt to help the best I can **What should be the optimal GC score cutoff** It sounds li…
Comment: Design matrix Differential expression analysis
by
SHN
▴ 40
Thank you for your explanation. I am working on the cell lines and I am investigating the effect of the treatment on the expression level, …
Answer: Issues with Mixture file when using CIBERSORTx
by
vjanve
• 0
I ran into same issue of "duplicated gene symbol(s)": 1: In CIBERSORTxFractions(sigmatrix = sigmatrix, mixture = mixture, : 22292 dup…
Comment: creating batch colum for batch correction
by
Expert
▴ 10
Thank you for your response. These samples were collected from the same patient at different time points(day 0, 8 and 18) and these are ind…
Comment: creating batch colum for batch correction
by
ATpoint
82k
Not sure I follow. Can you first of all describe which comparisons you want to make, pretending there was no batch effect at all. Write dow…
Comment: Pindel Segmentation Fault Error
by
Ruqaiya
• 0
This the tool still problematic?
Comment: Segmentation error while using a tool
by
Ruqaiya
• 0
Thank you for the advice, i'll keep that in mind.
Comment: Segmentation error while using a tool
by
Ruqaiya
• 0
I am trying to reproduce some bioinformatics data from a research paper-[this paper][1] as i was asked to work on an MRSA bioinfo project, …
Answer: Segmentation error while using a tool
by
Mensur Dlakic
★ 27k
Most likely you didn't do anything wrong. Going by your previous post that you have only 8 GB of RAM, the most likely reason is the lack of…
Comment: Segmentation error while using a tool
by
Thanujay S
• 0
Hey @Ruqaiya! Can you please give more details on why smalt is opted here? According to what I can see, the tool has become obsolete! Why …
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