updated just now by
GenoMax
151k
•
written 17 minutes ago by
rajdeepboral00
▴
70
0
votes
1
reply
11
views
0
votes
0
replies
22
views
2
votes
4
replies
137
views
updated 1 hour ago by
colindaven
7.6k
•
written 7 hours ago by
sarahmanderni
▴
130
5
votes
2
replies
1.3k
views
updated 2 hours ago by
ashotmarg2004
▴
130
•
written 21 months ago by
a615ebfb
▴
50
0
votes
1
reply
53
views
839
votes
170
replies
179k
views
112 follow
updated 6 weeks ago by
Biostar
3.6k
•
written 8.5 years ago by
Istvan Albert
102k
0
votes
0
replies
53
views
8 hours ago by
Physalia-courses
★
2.6k
0
votes
2
replies
117
views
0
votes
0
replies
68
views
0
votes
0
replies
74
views
0
votes
0
replies
85
views
21 hours ago by
zsc25001
▴
10
1
vote
6
replies
232
views
0
votes
0
replies
91
views
1 day ago by
Titus
▴
910
0
votes
3
replies
186
views
29 minutes ago by
rajdeepboral00
▴
70
0
votes
0
replies
79
views
1
vote
7
replies
273
views
updated 4 hours ago by
GenoMax
151k
•
written 1 day ago by
SpliceAndScript
•
0
1
vote
1
reply
146
views
updated 1 day ago by
GenoMax
151k
•
written 1 day ago by
Arup Ghosh
3.3k
0
votes
2
replies
192
views
13 hours ago by
Peter
•
0
0
votes
0
replies
114
views
2
votes
4
replies
279
views
0
votes
2
replies
198
views
1
vote
2
replies
220
views
4
votes
2
replies
208
views
0
votes
0
replies
134
views
0
votes
0
replies
110
views
0
votes
0
replies
115
views
2 days ago by
Begonia_pavonina
▴
210
5
votes
2
replies
215
views
2 days ago by
Mahdi&Christ
▴
20
4
votes
4
replies
324
views
9
votes
9
replies
497
views
0
votes
1
reply
196
views
0
votes
1
reply
709
views
2
votes
10
replies
476
views
updated 1 day ago by
GenoMax
151k
•
written 2 days ago by
anthony.santana.703.j
▴
30
0
votes
0
replies
152
views
2 days ago by
Claire Watson
▴
60
1
vote
6
replies
334
views
4
votes
0
replies
176
views
2
votes
0
replies
154
views
2
votes
0
replies
167
views
2
votes
0
replies
173
views
0
votes
2
replies
209
views
2
votes
7
replies
429
views
updated 2 days ago by
GenoMax
151k
•
written 3 days ago by
ramiro.barrantes
▴
60
1
vote
1
reply
189
views
0
votes
1
reply
187
views
0
votes
1
reply
190
views
1
vote
1
reply
235
views
1
vote
1
reply
223
views
updated 2 days ago by
rfran010
★
1.5k
•
written 3 days ago by
SpliceAndScript
•
0
0
votes
2
replies
224
views
updated 3 days ago by
dthorbur
★
3.0k
•
written 3 days ago by
SpliceAndScript
•
0
1
vote
1
reply
346
views
updated 3 days ago by
colindaven
7.6k
•
written 7 days ago by
emil.paulitz
•
0
0
votes
0
replies
196
views
0
votes
1
reply
238
views
0
votes
0
replies
230
views
Recent Votes
Recent Locations •
All
Italy, 3 minutes ago
Ireland, 5 minutes ago
United States, 5 minutes ago
United States, 10 minutes ago
Seattle, WA USA, 10 minutes ago
United States, 19 minutes ago
United States, 19 minutes ago
Recent Awards •
All
Popular Question to
QX
▴
70
Popular Question to
yura.grabovska
▴
780
Popular Question to
Begonia_pavonina
▴
210
Popular Question to
Morris_Chair
▴
370
Popular Question to
adarsh
▴
60
Popular Question to
Physalia-courses
★
2.6k
Popular Question to
Nicolas Rosewick
11k
Recent Replies
Comment: Y-axis break in Volcano Plot
by
GenoMax
151k
> Any help is appreciated
You should consider upvoting and validating (accepting, green check mark) comments/answers you have received on …
Comment: Isoform analysis after quantification with Salmon/Star
by
rajdeepboral00
▴
70
Thank you for your detailed reply
Answer: Nanopore data analysis
by
colindaven
7.6k
- Merge bams - yes. (why do you have so many ?). Merging fastqs before creating bams is easier (cat *.fastq.gz ) than bam merging.
- 150…
Answer: What is the difference between norm --multiallelics -any versus --atomize?
by
ashotmarg2004
▴
130
There is a related discussion here: https://github.com/samtools/bcftools/issues/2142
Answer: checking the sequences that share origin
by
GenoMax
151k
Without the presence of [**UMI**][1] in data, it would be difficult to say with certainty that a pair of sequences share an identical origi…
Comment: Bambu on illumina short reads sequencing
by
Rob
7.1k
I'll agree with GenoMax here. While the approach of bambu shares several methodological underpinnings with methods used for short-read qua…
Comment: Nanopore data analysis
by
GenoMax
151k
Data volume is going to depend on the amount of sequencing. Was this a PromethION flowcell? Tool should handle the data as long as you have…
Comment: Bambu on illumina short reads sequencing
by
GenoMax
151k
Did you see a recent thread --> https://www.biostars.org/p/9612865/ which delineates standard methods for short-read data.
Bambu is meant…
Comment: Nanopore data analysis
by
sarahmanderni
▴
130
The overall size for all bam files is around 150 GB. Is this common for nanopore and does this tools handle this volume of data?
Thanks fo…
Comment: Nanopore data analysis
by
GenoMax
151k
How about using `modkit` provided by Nanopore for methylation data analysis: https://github.com/nanoporetech/modkit
Comment: Significant reads loss after fastp
by
GenoMax
151k
Will refer you back to my comment above. Don't be fixated on the Q scores. You don't say what kind of data this is but it may still align f…
Comment: Better splitting of mutliallelic sites then bcftools norm --m-any
by
gernophil
▴
130
Because this question just received an upvote: I used `vt decompose -s` back in the days, but I think bcftools now also makes you choose th…
Comment: Significant reads loss after fastp
by
SpliceAndScript
•
0
I have attached the phred score image of my reads, your opinion will be appreciated.
Comment: Significant reads loss after fastp
by
SpliceAndScript
•
0
I have RNA-seq data and here I am attaching the fastqc report image of ![enter image description here][1]my reads before and after running …
Comment: Data of microarray
by
Peter
•
0
sorry but I do not have input file. If you have can you offer to me?
Traffic: 2898 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6