Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
1,000 results • Page
1 of 20
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
10
views
What is the bin size for Bamcompare?
bin
chipseq
size
bamcompare
deeptools
33 minutes ago by
Emily
▴ 20
0
votes
0
replies
19
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
44 minutes ago by
Holly
• 0
0
votes
0
replies
12
views
The total expressed genes in RNA-Seq data
RNA-SEQ
42 minutes ago by
Pegasus
▴ 100
2
votes
5
replies
94
views
What does the 'E%' represent in BUSCO results?
BUSCO
updated 34 minutes ago by
Philipp Bayer
8.5k • written 4 hours ago by
林明德
• 0
0
votes
4
replies
2.2k
views
MGLTools does not work in windows 11
Autodock
MGLTools
windows
windows11
updated 1 hour ago by
Ashfaq
• 0 • written 13 months ago by
mohyeddine.taleb
• 0
0
votes
0
replies
26
views
Overlapping Ranges within Granges object
Genomicranges
IRanges
GRanges
3 hours ago by
ntsopoul
▴ 60
0
votes
0
replies
32
views
Inquiry about deseq2 transformation
RNA-seq
deseq2
3 hours ago by
Chen
• 0
0
votes
0
replies
33
views
Is there any way to modify this pie chart ?
pie
ggplot
R
chart
3 hours ago by
ohtang7
▴ 40
0
votes
1
reply
58
views
Filtering based on alternate allelic balance
GATK
Bioinformatics
Filter
VCF
4 hours ago by
Arton
• 0
0
votes
0
replies
31
views
Biomart issue, why so few 3'utrs?
utr
biomart
4 hours ago by
RNAseqer
▴ 270
0
votes
4
replies
81
views
Help with IGV abbreviation
Genome
browser
3 hours ago by
GeneC
• 0
795
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
0
votes
1
reply
84
views
How to process Bulk WES data?
WES
WGS
updated 6 hours ago by
GenoMax
142k • written 7 hours ago by
wyuan37
• 0
0
votes
1
reply
85
views
Can diamond prepdb be used to make a taxonomically aware database?
blast
diamond
taxonomy
updated 5 hours ago by
GenoMax
142k • written 7 hours ago by
cedric.blais
• 0
0
votes
2
replies
367
views
News:
FINAL CALL: 8th Berlin Summer School in NGS Data Analysis - Only a few last places available
DNA-seq
variant-calling
RNA-seq
illumina
transcriptomics
8 hours ago by
David Langenberger
11k
0
votes
1
reply
82
views
Empty .best and .sing2 Files After Running Demuxlet
Biosciences
Demuxlet
updated 6 hours ago by
Ram
43k • written 8 hours ago by
eking28
• 0
1
vote
4
replies
346
views
featureCounts output summary assigned value higher than uniquely mapped reads from HISAT2
RNA-seq
featureCounts
HISAT
updated 8 hours ago by
GenoMax
142k • written 5 days ago by
Prawesh
• 0
1
vote
3
replies
308
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
9 hours ago by
kalavattam
▴ 190
0
votes
1
reply
140
views
How to interpret infinite odds ratio?
statistics
updated 9 hours ago by
bhumm
▴ 140 • written 15 hours ago by
Lukas
• 0
1
vote
2
replies
109
views
Duplicated sequence samtools
bowtie2
samtools
updated 10 hours ago by
GenoMax
142k • written 11 hours ago by
Moinuddin
• 0
0
votes
0
replies
56
views
reference-free assembly error assessment tools
assembly
11 hours ago by
lagartija
▴ 160
1
vote
2
replies
142
views
Benchmarking RNASeq Variant Calling Pipeline (Short Reads)
rna-seq
vcf
variant-calling
updated 11 hours ago by
lagartija
▴ 160 • written 16 hours ago by
Esraa
• 0
1
vote
0
replies
57
views
Extracting only 4-fold degenerate sites from gene sequences/alignments?
alignments
12 hours ago by
J.
▴ 10
0
votes
0
replies
69
views
Herald:
The Biostar Herald for Monday, May 13, 2024
herald
12 hours ago by
Biostar
2.7k
0
votes
1
reply
84
views
Splitting Seurat object by sample layers
seurat
updated 6 hours ago by
Ram
43k • written 12 hours ago by
kilcdincer
▴ 10
0
votes
3
replies
129
views
Galaxy StringTie error
stringtie
galaxy
updated 4 hours ago by
Mathew
▴ 130 • written 12 hours ago by
trkfs
• 0
0
votes
0
replies
60
views
dbNSFP sift scores integration
SIFT
VEP
dbNSFP
14 hours ago by
atariw
▴ 10
0
votes
1
reply
106
views
Mouse ribosome sequences in fasta format
fasta
mm10
rRNA
updated 15 hours ago by
GenoMax
142k • written 1 day ago by
octpus616
▴ 100
0
votes
1
reply
90
views
Getting the coding_sequence.fasta from the .gff file from the AUGUSTUS gene prediction.
augustus
annotation
assembly
genome
updated 15 hours ago by
Juke34
8.6k • written 15 hours ago by
Vijith
▴ 30
0
votes
1
reply
108
views
consensus sequence calling
consensus
updated 12 hours ago by
bk11
★ 2.4k • written 16 hours ago by
Ghada
• 0
0
votes
3
replies
132
views
Single-cell RNA-seq FindMarker and EnhancedVolcano Plot: most genes are low -log10P value
Seurat
single-cell
scRNA-seq
R
updated 6 hours ago by
Ram
43k • written 17 hours ago by
yau
• 0
3
votes
2
replies
221
views
imputation through beagle
panel
beagle
reference
imputation
11 hours ago by
analyst
▴ 50
0
votes
0
replies
58
views
Empty kernel in SEACells model
single-cell
scanpy
seacells
python
anndata
16 hours ago by
JACKY
▴ 140
0
votes
1
reply
374
views
Using samtools with GCS (google cloud storage) on a docker container seems to give "Protocol Not Supported" error
samtools
gcs
updated 17 hours ago by
aw7
▴ 280 • written 24 days ago by
abhishekghadge
• 0
1
vote
1
reply
170
views
Long reads and fixing of mate-pair issues/marking duplicates with samtools
mate-pair
samtools
alignment
updated 17 hours ago by
aw7
▴ 280 • written 5 days ago by
Zeng Hao
▴ 40
0
votes
0
replies
70
views
Reference panel of normals for ensembl named refgenome
Mutect2
18 hours ago by
gernophil
▴ 80
1
vote
0
replies
68
views
Producing sequence for splicing isoforms
isoform
rMATs
splicing
alternative
SplAdder
18 hours ago by
tomas4482
▴ 400
2
votes
3
replies
210
views
BWA alignment
Samtools
bam
19 hours ago by
Vahid
• 0
3
votes
2
replies
139
views
Publish a Letter in higher impact vs Research article in lower impact
Article
Letter
Journal
updated 19 hours ago by
ATpoint
82k • written 19 hours ago by
jennyp0706
• 0
0
votes
1
reply
130
views
Manual Assembly and Protein Translation, HELP, assignment revision
sequence
university
assembly
protein
genomics
updated 20 hours ago by
Philipp Bayer
8.5k • written 1 day ago by
samRayne
• 0
0
votes
1
reply
214
views
Two-Sample Mendelian Randomization: Association between Instrumental Variable and Outcome
Mendelian-Randomization
updated 6 hours ago by
Ram
43k • written 4 days ago by
Nikki
• 0
2
votes
13
replies
2.8k
views
6 follow
CNVKIT - unable to produce scatter and diagram pdfs
cnvkit
updated 22 hours ago by
Anitha
• 0 • written 4.8 years ago by
ww22runner
▴ 60
0
votes
1
reply
167
views
Generating .bed file and .map file for polyploid vcf file through plink
bed
polyploid
map
plink
updated 22 hours ago by
chrchang523
10k • written 5 days ago by
analyst
▴ 50
0
votes
0
replies
84
views
lncRNA
tcga
lncrna
23 hours ago by
jain72744
▴ 10
0
votes
1
reply
211
views
Nomalization - TCGA, RNA-seq and Microarray
TCGA
RNA-seq
Nomalization
Microarray
updated 1 day ago by
Zhenyu Zhang
★ 1.2k • written 3 days ago by
jain72744
▴ 10
2
votes
5
replies
519
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 1 day ago by
arctic
▴ 40 • written 10 days ago by
M.
▴ 30
1
vote
2
replies
302
views
How to remove multiple batch effects from RNA-seq data before limma differential gene expression analysis?
ComBat-seq
limma
RNA-seq
removeBatchEffect
batch-effect
updated 12 hours ago by
Ram
43k • written 1 day ago by
t.fortunato.asquini
• 0
2
votes
1
reply
233
views
Practical Haplotype Graph v2 not finding correct paths
Pangenome
PHG
graph
updated 1 day ago by
pjb39
▴ 210 • written 3 days ago by
beantkapoor16
▴ 10
0
votes
2
replies
185
views
Output file of samtools flagstat empty
samtools-flagstat
updated 19 hours ago by
colindaven
6.4k • written 1 day ago by
ramendra.sarma
• 0
0
votes
0
replies
99
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
eigencorplot
PCAtools
deseq2
pca
1 day ago by
BioinfGuru
★ 1.7k
1,000 results • Page
1 of 20
Recent Votes
A: How can I convert -log10 (p-value) to p-value?
Comment: What does the 'E%' represent in BUSCO results?
Answer: What does the 'E%' represent in BUSCO results?
Answer: Combine Two Affymetrix Datasets With Same Platform
Comment: Kraken2 database
Comment: How does gene length effect the number of reads mapped
Comment: How does gene length effect the number of reads mapped
Recent Locations •
All
Australia,
1 minute ago
Singapore,
2 minutes ago
United States,
2 minutes ago
United States,
2 minutes ago
France,
8 minutes ago
Singapore,
8 minutes ago
Australia,
14 minutes ago
Recent Awards •
All
Autobiographer
to
Jeol
▴ 20
Popular Question
to
Pegasus
▴ 100
Popular Question
to
RNAseqer
▴ 270
Scholar
to
kalavattam
▴ 190
Scholar
to
dthorbur
★ 2.0k
Commentator
to
Santosh Anand
5.7k
Popular Question
to
lagartija
▴ 160
Recent Replies
Comment: What does the 'E%' represent in BUSCO results?
by
Philipp Bayer
8.5k
No worries! I found the commit with the maths too: https://gitlab.com/ezlab/busco/-/blame/master/src/busco/busco_tools/hmmer.py?page=2#L135…
Comment: What does the 'E%' represent in BUSCO results?
by
林明德
• 0
Thank you for your answer! It really helped me resolve a doubt!
Answer: What does the 'E%' represent in BUSCO results?
by
Philipp Bayer
8.5k
Ah your edit solved this. 50 BUSCOs contain internal stop codons, indicating they might be erroneous gene models, or the genes themselves a…
Answer: MGLTools does not work in windows 11
by
Ashfaq
• 0
In my case, I did not see iGPU. There is only one option, Intel UHD Graphics. What I need to do?
Comment: What does the 'E%' represent in BUSCO results?
by
Philipp Bayer
8.5k
funny enough i tried the same thing (i've used BUSCO heaps times but never saw the E:! even the manual lists 'C:89.0%[S:85.8%,D:3.2%],F:6.9…
Comment: What does the 'E%' represent in BUSCO results?
by
GenoMax
142k
According to ChatGPT: In BUSCO, the "E" category represents "End" or "Endof" gene fragments. These are orthologous groups for which the ge…
Comment: Help with IGV abbreviation
by
GeneC
• 0
Thank you for the link and details
Comment: Help with IGV abbreviation
by
GeneC
• 0
Thank you very much for helping with the details.
Comment: How does gene length effect the number of reads mapped
by
Chen
• 0
thank you :)
Answer: Filtering based on alternate allelic balance
by
Arton
• 0
I found the answer when using bcftools. Is there is a way to do this with FilterVcf? bcftools filter --include '(FMT/AD[0:1])/(FMT/A…
Comment: Help with IGV abbreviation
by
GenoMax
142k
Those are SAM format fields. Check section 1.4 here: https://samtools.github.io/hts-specs/SAMv1.pdf > 1:2114:12111:13792 That is part of …
Answer: Help with IGV abbreviation
by
Mathew
▴ 130
Hi, here is a breakdown of each part that you asked about: **flag 99**: This indicates various properties of the read alignment. In this c…
Comment: Kraken2 database
by
Mathew
▴ 130
I don't see any databases with just pathogenic bacteria genomes from just a quick search, I would imagine that using the Standard-16 databa…
Comment: Galaxy StringTie error
by
Mathew
▴ 130
I am not sure if this is true for Galaxy, but when I code in Python, SyntaxWarning: invalid escape sequence '' occurs when you want to add …
Comment: Can diamond prepdb be used to make a taxonomically aware database?
by
GenoMax
142k
Should be possible: https://www.biostars.org/p/430366/ Are you using an older version of `diamond`? Latest versions of `diamond` can use N…
Traffic: 1682 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6