21 minutes ago by
Mohamed Samir
▴
30
0
votes
0
replies
7
views
0
votes
2
replies
56
views
5 minutes ago by
resug
▴
30
0
votes
0
replies
23
views
2 hours ago by
Kinoppy
•
0
2
votes
3
replies
149
views
808
votes
167
replies
146k
views
109 follow
updated 11 days ago by
Biostar
2.9k
•
written 7.5 years ago by
Istvan Albert
101k
0
votes
0
replies
76
views
2
votes
9
replies
607
views
7 follow
0
votes
0
replies
47
views
0
votes
0
replies
50
views
0
votes
3
replies
132
views
updated 1 hour ago by
WouterDeCoster
47k
•
written 18 hours ago by
sewaw37819
•
0
0
votes
0
replies
76
views
18 hours ago by
sivan.kinreich
•
0
0
votes
0
replies
72
views
19 hours ago by
Daniel
•
0
0
votes
0
replies
65
views
updated 18 hours ago by
GenoMax
143k
•
written 19 hours ago by
diqixiaoyaoer
▴
20
2
votes
2
replies
162
views
5 hours ago by
kmyers2
▴
80
0
votes
1
reply
100
views
updated 22 hours ago by
bhumm
▴
150
•
written 22 hours ago by
ramiro.barrantes
•
0
0
votes
2
replies
141
views
1 day ago by
kewoka9286
•
0
2
votes
9
replies
250
views
0
votes
1
reply
105
views
3
votes
4
replies
195
views
17 hours ago by
Jaskaran Singh
•
0
1
vote
1
reply
183
views
updated 1 day ago by
Ram
44k
•
written 3 days ago by
QuotoAstro
•
0
0
votes
0
replies
86
views
0
votes
1
reply
126
views
5
votes
7
replies
244
views
4
votes
4
replies
319
views
updated 1 day ago by
Ram
44k
•
written 2 days ago by
Nour-eddine
▴
30
0
votes
0
replies
89
views
updated 1 day ago by
Ram
44k
•
written 1 day ago by
Mohamed Abderrahmane
▴
20
0
votes
0
replies
89
views
0
votes
2
replies
431
views
2
votes
3
replies
259
views
0
votes
0
replies
104
views
2
votes
3
replies
211
views
0
votes
4
replies
262
views
updated 1 day ago by
Mensur Dlakic
★
27k
•
written 2 days ago by
AINUR FATIN BINTI MAZRI
•
0
0
votes
1
reply
138
views
0
votes
1
reply
251
views
0
votes
2
replies
203
views
updated 1 day ago by
Pierre Lindenbaum
162k
•
written 1 day ago by
María José
•
0
1
vote
1
reply
305
views
1
vote
3
replies
209
views
0
votes
1
reply
142
views
2
votes
2
replies
204
views
1 day ago by
solo.albif
•
0
1
vote
2
replies
219
views
1 day ago by
bio_info
▴
20
0
votes
0
replies
121
views
updated 2 days ago by
Ram
44k
•
written 2 days ago by
carlopecoraro2
★
2.5k
2
votes
3
replies
424
views
0
votes
0
replies
117
views
2 days ago by
gogeni5529
▴
50
12
votes
13
replies
6.0k
views
7 follow
updated 2 days ago by
Johan Zicola
▴
70
•
written 4.7 years ago by
Matus
▴
70
2
votes
2
replies
226
views
updated 2 days ago by
Ram
44k
•
written 3 days ago by
sc_analysis
•
0
0
votes
0
replies
127
views
updated 2 days ago by
Ram
44k
•
written 2 days ago by
panacotaforcota
•
0
4
votes
3
replies
213
views
updated 2 days ago by
ATpoint
83k
•
written 2 days ago by
sarahmanderni
▴
110
0
votes
2
replies
192
views
1
vote
1
reply
176
views
4
votes
2
replies
276
views
updated 2 days ago by
a.alnawfal.1992
▴
290
•
written 3 days ago by
Bindiya
▴
10
Recent Votes
Recent Locations •
All
United Kingdom, just now
USA, 6 minutes ago
Canada, 11 minutes ago
United States, 22 minutes ago
Finland, 27 minutes ago
Egypt, 29 minutes ago
Thailand, 31 minutes ago
Recent Awards •
All
Popular Question to
asalimih
▴
60
Popular Question to
devenvyas
▴
750
Teacher to
Kevin Blighe
88k
Scholar to
chrchang523
10k
Popular Question to
pinheirofabiano
▴
20
Popular Question to
Leendert
▴
30
Recent Replies
Comment: how linearize nucleotide lines in axt file
by
resug
▴
30
There are two sequences per entry, and the provided awk script has merged the two sequences into a single linear sequence. But the objectiv…
Answer: how linearize nucleotide lines in axt file
by
GenoMax
143k
Using @yokofakun 's [**linearize fasta code**][1]
awk '/^Lm/ {printf("%s%s\t",(N>0?"\n\n":""),$0);N++;next;} {printf("%s",$0);} END …
Comment: Help aligning Nanopore Long Read RNA seqs
by
WouterDeCoster
47k
That is very few reads. Are you sure your run was successful?
Comment: Alignment tool for very short reads (25bp)
by
ATpoint
83k
bowtie aka bowtie1 was developed when these very short reads were state of the art. Id give that a try and see what comes out. Multithreade…
Answer: Alignment tool for very short reads (25bp)
by
Jeremy Leipzig
22k
> What are other reliable tools for the alignment of DNA reads with very short lengths (25-50bp)?
[vmatch][1]
[1]: http://www.vma…
Comment: edgeR batch effect model matrix sanity check
by
kmyers2
▴
80
Thank you! Appreciate your reply!
Comment: blast Segmentation fault
by
Tina
•
0
I used 'blastn-short', but it still resulted in a segmentation fault. I downloaded the 'nt_euk' index from NCBI ([enter link description he…
Comment: Help aligning Nanopore Long Read RNA seqs
by
sewaw37819
•
0
> What percentage of your library do you expect to be "long-non-coding transcripts"?
A higher percentage than what we are seeing right now…
Comment: Failed to download data from EBI with ascp
by
Michael
54k
Please check my answer. No need to send an email to EBI either.
Answer: Failed to download data from EBI with ascp
by
Michael
54k
**Anonymous Aspera download from ENA works as expected.**
Please follow the official documentation https://ena-docs.readthedocs.io/en/late…
Answer: Alignment tool for very short reads (25bp)
by
Jack Tierney
▴
360
Kudos for writing such a clear and well formatted question. I will give my perspective but don't claim to be an authority. Full disclosure …
Comment: Failed to download data from EBI with ascp
by
ATpoint
83k
Please email EBI and dont post duplicates. Especially not as an answer.
Comment: Help aligning Nanopore Long Read RNA seqs
by
rfran010
▴
950
What percentage of your library do you expect to be "long-non-coding transcripts"?
> (though there IS some coverage, but mostly on whole…
Comment: Overrepresented sequence in reverse read
by
Jaskaran Singh
•
0
Thank You so much....it was really helpful...i was able to remove the poly G with fastp
Comment: Overrepresented sequence in reverse read
by
Jaskaran Singh
•
0
Hi,
Thanks, it was helpul information. I used fastp to remove the poly g and the QC seems to be fine now.
Traffic: 1230 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6