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184 results • Page
4 of 4
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Votes
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0
votes
1
reply
156
views
RNA-seq bacteria contamination
RNA-seq
updated 6 days ago by
GenoMax
141k • written 6 days ago by
sh
• 0
0
votes
2
replies
195
views
Result of running of p3_in.pl
primer3
updated 6 days ago by
GenoMax
141k • written 6 days ago by
nasri
• 0
0
votes
1
reply
136
views
FAM FILE
file
Fam
6 days ago by
Eleonora
• 0
0
votes
0
replies
74
views
Imputation advice
imputation
6 days ago by
kl
▴ 10
3
votes
5
replies
279
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 6 days ago by
GenoMax
141k • written 6 days ago by
ahmad.sajad4541
• 0
0
votes
0
replies
92
views
Trimming best practices for SNP calling
trimming
SNP
trimmomatic
6 days ago by
Enrique
• 0
3
votes
5
replies
556
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
6 days ago by
n_navy
• 0
0
votes
0
replies
75
views
Correct way of reducing predictive model complexity
lasso
glmnet
predictvemodeling
regression
6 days ago by
sativus
▴ 20
6
votes
3
replies
185
views
Interpretting IGV output
IGV
updated 6 days ago by
Carlo Yague
8.7k • written 6 days ago by
analyst
▴ 30
0
votes
1
reply
178
views
homology modelling using swiss model
homology-modeling
swiss-model
updated 6 days ago by
colindaven
6.4k • written 7 days ago by
Ayush
• 0
0
votes
0
replies
82
views
Difficulties getting **the adjacency file** necessary for **aracne2regulon** function.
VIPER
MARINA
ARACNe
6 days ago by
Biostars2200
• 0
1
vote
5
replies
350
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
5 days ago by
anasjamshed
▴ 120
0
votes
0
replies
132
views
CIRIquant: ValueError: file has no sequences defined (mode='rb') - is it SAM/BAM format? Consider opening with check_sq=False
Ciriquant
updated 6 days ago by
Carlo Yague
8.7k • written 6 days ago by
Atul K.
• 0
3
votes
2
replies
278
views
p-value and Boneferroni adjusted p-value the same in R
Bonferroni
R
Statistics
p-value
Fisher
6 days ago by
kristiewyleung
• 0
2
votes
5
replies
458
views
Marking duplicates using UMIs
Deduplication
UMI
updated 6 days ago by
i.sudbery
19k • written 7 days ago by
Lipika
• 0
3
votes
4
replies
670
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
6 days ago by
rahu
• 0
0
votes
0
replies
206
views
traying to make a maf from an already annotated vcf
vcf
vcf2maf
maf
maftools
VEP
6 days ago by
Javier
• 0
0
votes
0
replies
195
views
Job:
Ensembl Outreach Project Leader
ebi
embl
genomics
ensembl
6 days ago by
Ben_Ensembl
★ 2.4k
3
votes
6
replies
661
views
Ciriquant not configuring hisat2 indexed files
ciriquant
6 days ago by
Atul K.
• 0
1
vote
2
replies
240
views
permutation test in edgeR
rna-seq
edgeR
updated 6 days ago by
Gordon Smyth
★ 7.0k • written 20 days ago by
Netanel
• 0
0
votes
6
replies
440
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 4 days ago by
gv
• 0 • written 27 days ago by
Srinka
▴ 20
4
votes
2
replies
376
views
Missing protein (VEGF-A) in String db
VGFA
stringdb
updated 2 days ago by
shalespringer
• 0 • written 11 weeks ago by
Phenylananin
▴ 20
0
votes
2
replies
589
views
Why cd-hit-est not work when sequence identity threshold<0.95?
genome
cluster
sequence
updated 3 days ago by
Dateline
• 0 • written 21 months ago by
JZX
• 0
1
vote
5
replies
603
views
Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Series Library Preparation Set?
cell-barcode
MGI
DNBelab
single-cell
updated 5 days ago by
atowns21
• 0 • written 3 months ago by
benjamin.pyenson
• 0
1
vote
4
replies
431
views
GTF file for Rhinolophus sinicus
Rhinolophus-sinicus
GTF
updated 4 days ago by
atowns21
• 0 • written 5 months ago by
ara
• 0
2
votes
3
replies
955
views
Method to detect genome doubling
CNV
genome doubling
updated 6 days ago by
LauferVA
4.2k • written 4.5 years ago by
CY
▴ 750
0
votes
0
replies
275
views
Choice of relatedness cutoff for GWAS of a large cohort (>100K samples)
relatedness
IBD
GWAS
updated 6 days ago by
Ram
43k • written 14 months ago by
Oak
▴ 10
3
votes
3
replies
2.3k
views
How to make a TSS enrichment plot in ATAC-seq analysis?
ATAC
updated 3 days ago by
sp
• 0 • written 22 months ago by
Dan
▴ 180
2
votes
3
replies
2.5k
views
When to use .vcf or .gvcf files from GATK HaplotypeCaller?
indel
gatk
calling
snp
variant
updated 18 hours ago by
zihanss
• 0 • written 23 months ago by
Vitor1
▴ 120
2
votes
6
replies
3.7k
views
Treatment VS Control in Single Cell RNAseq analysis
Single Cell
RNAseq
DE Analysis
Treatment
Control
updated 3 days ago by
ATpoint
82k • written 3.9 years ago by
grayapply2009
▴ 280
7
votes
7
replies
5.9k
views
6 follow
How to know synonymous and non-synonymous SNPs from transcriptome sequencing
RNA-Seq
SNP
updated 3 days ago by
rohitsatyam102
▴ 850 • written 6.1 years ago by
smallfish
▴ 10
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 5 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
10
votes
6
replies
9.9k
views
6 follow
CDS vs cDNA vs transcript for mapping RNA-Seq reads
Assembly
rna-seq
alignment
updated 2 days ago by
Antonio R. Franco
★ 5.1k • written 6.0 years ago by
williamsbrian5064
▴ 510
14
votes
2
replies
7.0k
views
Trouble with Pilon installation
polishing
pilon
installation
updated 3 days ago by
Lissa Cruz Saavedra
• 0 • written 7.3 years ago by
Rox
★ 1.4k
184 results • Page
4 of 4
Recent Votes
Comment: NGS forensics: how to know if data is fabricated
Answer: How to use limma to find differentially expressed genes in response to a continu
Batch effects : ComBat or removebatcheffects (limma package) ?
How to input margin to UpsetR figure
Comment: How to assign cell types after integration in scRNA
NGS forensics: how to know if data is fabricated
Answer: ChIP-seq datasets: input samples omitted?
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Recent Replies
Comment: What does it mean single base resolution in sequencing?
by
dsull
★ 5.9k
Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
Answer: What does it mean single base resolution in sequencing?
by
dsull
★ 5.9k
I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
Answer: Cannot process all the reads in a fast5 file?
by
chujie
• 0
Hi, I met the same problem, have you found the solution?
Answer: What does it mean single base resolution in sequencing?
by
LauferVA
4.2k
Hi @fe3e6f65 , In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
Comment: PCA plot
by
LauferVA
4.2k
Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
Comment: NGS forensics: how to know if data is fabricated
by
Jeremy Leipzig
22k
i have the name ready: out**liar**
Comment: What does it mean single base resolution in sequencing?
by
GenoMax
141k
Sequencing by hybridization (https://en.wikipedia.org/wiki/Sequencing_by_hybridization ) is likely not giving you single base resolution. B…
Comment: Fetch table from clinvar database according to a list of rsid
by
ashaneev07
▴ 20
Hii... i have updated the script in python..But, still getting no data found. Actually the data is there, i have print the parsed html file…
Comment: How to assign cell types after integration in scRNA
by
ATpoint
82k
https://bioconductor.org/books/release/OSCA.multisample/using-corrected-values.html
Comment: Downloading full alignments from Pfam
by
GenoMax
141k
Not as convenient but the entire set of full alignments can be downloaded here: https://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pf…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
> What does clipped Fastq mean? Probably means that NCBI has already scanned and trimmed adapter sequencers. > both forward and revers…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
You can use the default `adapters.fa` file included in the `resources` folder of [**BBMap suite**][1] (program to use is `bbduk.sh`) or a p…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
Only way to find out is to try. It may work but if it is not going to then you will find that out quick (process would likely crash because…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
Also, there is no mention of adapter sequence so which adapter sequence should i use to remove it from some other reads?
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
Also, Do you think i can reproduce at least most part of the data from the paper just on my laptop? It has 4 logical processors (Intel(R) C…
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