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112,714 results • Page
3 of 2255
Sort: Rank
Rank
Views
Votes
Replies
0
votes
1
reply
148
views
Download an example of fully-imputed VCF files ?
VCF
impute
updated 4 days ago by
barslmn
★ 1.8k • written 4 days ago by
Ben
• 0
0
votes
1
reply
166
views
How to find the most representative protein data base ID for your protein?
modelling
protein
database
updated 4 days ago by
GenoMax
134k • written 5 days ago by
amy__
▴ 150
0
votes
2
replies
191
views
Visualize where kmers are on a reference genome
kmer
visualize
updated 4 days ago by
Alex Reynolds
35k • written 4 days ago by
Carrie
• 0
7
votes
7
replies
384
views
Filter human transcription factors
transcription-factor
RNA-seq
updated 3 days ago by
jared.andrews07
★ 15k • written 4 days ago by
Chris
▴ 200
2
votes
6
replies
300
views
obtaining circular RNAs' sequences from circBase
circular-RNA
2 days ago by
aminijavad4
▴ 10
0
votes
0
replies
120
views
How to annotate BED for tissue expression?
BED
annotation
4 days ago by
Fabio_bie88
• 0
2
votes
0
replies
158
views
Job:
Human Genomics Team Leader
ebi
ensembl
embl
genomics
4 days ago by
Ben_Ensembl
★ 2.3k
0
votes
1
reply
1.7k
views
error when running local chlorop1.1
chlorop
updated 4 days ago by
Ram
40k • written 7.3 years ago by
gerrychang1985
▴ 40
3
votes
14
replies
1.9k
views
10 follow
Help writing code for a question on my homework
biopython
updated 3 days ago by
Joe
21k • written 2.4 years ago by
hannahcheyenna
• 0
1
vote
1
reply
234
views
bedGraph for Coverage of Insert from Paired-End Data?
genome
awk
coverage
bed
paired-end
updated 4 days ago by
shelkmike
▴ 980 • written 7 days ago by
vanbelj
▴ 40
0
votes
6
replies
277
views
gene correlations in between two groups
gene-expression
correlation
3 days ago by
biology_inform
▴ 40
0
votes
1
reply
123
views
Running Ibex on both BCR chains?
BCR
ibex
single-cell
updated 4 days ago by
Ram
40k • written 5 days ago by
actinia94
• 0
2
votes
9
replies
472
views
Calculation of TMB on gene level
genomics
updated 3 days ago by
svp
▴ 590 • written 5 days ago by
smrutimayipanda
▴ 20
0
votes
0
replies
111
views
Spike-in normalization in ATAC-Seq with DiffBind
ATAC-Seq
spike-in
DiffBind
5 days ago by
frueher
• 0
0
votes
1
reply
240
views
News:
LAST CALL: Online Course - A Practical Introduction to NGS Data Analysis - October 23-25, 2023
DNA-seq
Workshop
RNA-seq
5 days ago by
David Langenberger
10k
1
vote
2
replies
156
views
Is it normal if regress out the cell cycle effects but the DEGs are quite the similar (no big changes)
regress_out
Cell_cycle
Seurat
4 days ago by
alwayshope
▴ 30
1
vote
9
replies
383
views
how to identify uniq genes between two gff files.
Genes
Uniq
Annotation
GFF
2 days ago by
nikhil
▴ 20
1
vote
3
replies
416
views
I have performed Trimmomatic run for adapter removal. QC report shows drop in the reads and presence of overrepresented sequences. Seeking help!
NGS
bioinformatics
illumina
WGS
updated 4 days ago by
swbarnes2
13k • written 5 days ago by
Vijith
▴ 30
0
votes
0
replies
113
views
filtering variants in a Strelka2 VCF file based on AD and AF
Filter
VCF
5 days ago by
Bogdan
★ 1.4k
2
votes
4
replies
269
views
Integrated Seurat object change name of the two conditions
R
integrated
Seurat
4 days ago by
camillab.
▴ 140
0
votes
4
replies
344
views
Hugo_Symbol to Entrez ID
hugo
bioinformatics
entrez
ids
symbols
updated 5 days ago by
Kevin Blighe
86k • written 7 days ago by
shakyaram079
• 0
2
votes
5
replies
304
views
How to change from gene_id to GeneID/EntrezID using R
identifiers
Deseq2
GeneID
updated 5 days ago by
Kevin Blighe
86k • written 6 days ago by
Nicolas
• 0
0
votes
4
replies
730
views
Is there a way to get a list of all homogygous sites for each sample with plink (regardless if they are in a run)
plink
updated 5 days ago by
chrchang523
10k • written 20 days ago by
curious
▴ 730
1
vote
4
replies
259
views
How to sort cd-hit-est cluster file
sort
cd-hit-est
4 days ago by
Mo
▴ 40
1
vote
5
replies
285
views
Enrichment of mitochondrial and ribosomal pathways - an artifact?
Pathway
Mitochondria
Ribosome
scRNA-seq
Enrichment
3 days ago by
omer.shomrat
• 0
13
votes
10
replies
658
views
8 follow
scRNAseq Differential expression analysis
scRNA-seq
Differential-Expression
Seurat
updated 5 days ago by
ATpoint
77k • written 8 days ago by
MVJ
▴ 10
3
votes
4
replies
296
views
Dotplot : how to self-define the range of legend
analysis
seq
RNA
updated 4 days ago by
GenoMax
134k • written 6 days ago by
Xuhao
• 0
0
votes
0
replies
113
views
News:
RADseq data analysis course
RADseq
Phylogenomics
Bioinformatics
Populationgenomics
Stacks
6 days ago by
carlopecoraro2
★ 2.4k
3
votes
2
replies
382
views
How to create structural variants ground truth for alignment of two long-read genome assemblies?
yeast
assembly
structural-variation
SV-callers
6 days ago by
Thomas
▴ 20
0
votes
1
reply
207
views
Merging the filename with tsv files for master file
genomics
updated 4 days ago by
barslmn
★ 1.8k • written 6 days ago by
smrutimayipanda
▴ 20
4
votes
6
replies
371
views
Hisat2 index and alignment question
ubuntu
rna-seq
index
hisat2
updated 3 days ago by
Istvan Albert
98k • written 6 days ago by
Athena
• 0
2
votes
4
replies
352
views
Ties in reranked list
fgsea
GSEA
6 days ago by
Hamza
• 0
1
vote
5
replies
470
views
Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempting To Use CuffDiff
CuffDiff
Bash
Cufflinks
14 hours ago by
Y
• 0
0
votes
0
replies
152
views
My rMATS output file is not showing gene symbols and only XLOC gene ID are being shown.
rMATS
rMATS-turbo
6 days ago by
Nipan
• 0
2
votes
4
replies
304
views
Using STAR aligner to build index of hg38
hg38
STAR
alignment
RNAseq
index
6 days ago by
Grace
• 0
0
votes
0
replies
159
views
Convert RNASeq V2 data in cBioPortal having RSEM normalized results to TPM values
bioinformatics
TPM
RNASeqV2
cbioportal
RSEM
updated 6 days ago by
4galaxy77
2.8k • written 7 days ago by
shakyaram079
• 0
0
votes
2
replies
278
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
6 days ago by
Mo
▴ 40
1
vote
2
replies
256
views
Understanding TCGA barcodes with dot in the fieldname
tcga
updated 4 days ago by
Zhenyu Zhang
▴ 980 • written 7 days ago by
Tahsin
• 0
0
votes
0
replies
161
views
Compare peaks between clusters in sc-ATAC
Peak-Calling
Epigenome
scATAC-seq
updated 7 days ago by
Ram
40k • written 7 days ago by
Ahmed.waraky
▴ 10
1
vote
3
replies
363
views
How to DESeq2 using miRNA data obtained using TCGAbiolinks
R
TCGAbiolinks
DESeq2
TCGA
updated 7 days ago by
Zhenyu Zhang
▴ 980 • written 9 days ago by
Mo
• 0
0
votes
7
replies
519
views
Highly inflated p-values in GWAS by regenie
regenie
plink
gwas
5 days ago by
cwwong13
▴ 20
1
vote
3
replies
282
views
How to find out what adapters to remove after FastQC of RNAseq data?
Trimmomatic
FastQC
NGS
RNA-seq
5 days ago by
ella
• 0
2
votes
5
replies
347
views
Trimmomatic .jar not included anymore?
trimmomatic
ubuntu
linux
updated 7 days ago by
petebio
▴ 100 • written 7 days ago by
Emily
▴ 30
0
votes
1
reply
190
views
News:
EMBL-EBI virtual course | Introduction to RNA-seq and functional interpretation
RNA-seq
updated 7 days ago by
Ram
40k • written 7 days ago by
Rebecca
• 0
0
votes
4
replies
580
views
Weirdness in annotation (missing allele frequencies)
allele-frequency
gnomad
annovar
updated 6 days ago by
Jeremy Leipzig
21k • written 7 days ago by
Can Abdullah
• 0
1
vote
3
replies
240
views
Finding sequences in unannotated genomes using reference coordinates
consensus
VCF
updated 7 days ago by
Ram
40k • written 7 days ago by
Prangan
▴ 20
0
votes
1
reply
220
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 7 days ago by
Ram
40k • written 8 days ago by
Mat
▴ 60
2
votes
5
replies
353
views
How to check RNAseq support for annotated genes?
gene
RNA-seq
annotation
updated 3 days ago by
Michael
53k • written 8 days ago by
BioinfoBee
• 0
0
votes
0
replies
349
views
Why dbConnect GEOmetadb_demo.sqlite shows some information while dbConnect GEOmetadb.sqlite shows no information
R
GEO
GEOmetadb
updated 7 days ago by
Pierre Lindenbaum
157k • written 8 days ago by
nonaddldy
▴ 10
1
vote
0
replies
169
views
News:
Online Training - Bioinformatics Pipeline Development with Nextflow (November 15-17, 2023)
nextflow
automation
pipeline
development
8 days ago by
David Langenberger
10k
112,714 results • Page
3 of 2255
Recent Votes
Comment: ncbi error report log for validate fastq issue
Comment: ncbi error report log for validate fastq issue
A: Bowtie2 MAPQ difference using local vs end-to-end alignment
C: Bowtie2 MAPQ difference using local vs end-to-end alignment
Answer: TPM RNA-seq data for differential expression analysis
A: Error When Installing Emboss
Answer: Subclustering of intergated cells from scRNA-seq data
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Recent Replies
Comment: Getting the overlap between two GTF files
by
GenoMax
134k
Something in `AGAT` should work: https://agat.readthedocs.io/en/latest/?badge=latest
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Pierre Lindenbaum
157k
> My problem has been with trying to loop the process. ok here is a **nextflow** based solution,(NOT tested) workflow { …
Comment: Assessing Rockhopper's output
by
langziv
▴ 50
I see. I tried using the GUI Rockhopper for RNA-seq analysis of a K. pneumoniae strain, but the program didn't find the strain's assembly…
Answer: Getting the overlap between two GTF files
by
Pierre Lindenbaum
157k
bedtools intersect \ -a <(awk '/^[^#]/ {printf("%s\t%d\t%s\t%s\n",$1,int($4)-1,$5,$0);}' file1.gtf | sort -t $'\t' -k1,1 -k2…
Comment: Assessing Rockhopper's output
by
shelkmike
▴ 980
I used Rockhopper only for bacterial genomes assembled into circular contigs or circular scaffolds.
Comment: Unable to install HorvathMammalMethylChip40manifest packages
by
ATpoint
77k
There is no package of that name in CRAN or Bioconductor. Where do you have it from, so which tutorial you follow?
Comment: Subclustering of intergated cells from scRNA-seq data
by
fifty_fifty
▴ 60
thank you. This is exactly what I was looking for
Answer: Search RCSB with a list of protein names?
by
Mensur Dlakic
★ 25k
This is not the most elegant solution, but it should work with previous suggestions. In this remote directory: http://ftp.wwpdb.org/p…
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Ram
40k
The manual says you can split samples out at once using the `+split` plugin. So, `bcftools +split -Oz -o <PATH> file.vcf.gz` should do the …
Comment: differences between trajectories in conditions with Monocle3 or other tools
by
Amitm
★ 2.2k
Phate (or Monocle) doesn't do UMAP. If you have done UMAP already (using seurat) then you could use the cell barcode to UMAP cluster label …
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I figured out how to make it work. The bams have to be comma separated not just by space: cd "${input_directory_with_associated_bam_fi…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
Hey, this is so close to what I'm looking for - I clarified in my edit. This is a great way to batch download lots of PDBs in RCSB by their…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
This is a really neat package, but I don't think it does exactly what I want, so I've clarified my question. This seems like it grabs a PD…
Comment: How to get the gft file to run velocyto for velocity analysis?
by
Chris
▴ 200
Yes, thank you! I figured it out but I got an error: velocyto run10x -m Hg38_rmsk.gtf WT/outs /cellranger/reference/refdata-cellranger…
Comment: How to get the gft file to run velocyto for velocity analysis?
by
bk11
★ 1.3k
You can get from here- [UCSC][1]. Just click on get output botton. [1]: https://genome.ucsc.edu/cgi-bin/hgTables?hgsid=611454127_NtvlaW…
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