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181 results • Page
1 of 4
Sort: Views
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Views
Votes
Replies
76
votes
72
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
2 days ago by
Kevin Blighe
87k
19
votes
11
replies
14k
views
10 follow
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated 1 day ago by
cmdcolin
★ 3.8k • written 10.8 years ago by
J.F.Jiang
▴ 920
2
votes
5
replies
10k
views
Find Corresponding Author: Doi Metadata?
updated 6 days ago by
Ram
43k • written 10.6 years ago by
Louis
▴ 150
10
votes
6
replies
9.8k
views
6 follow
CDS vs cDNA vs transcript for mapping RNA-Seq reads
Assembly
rna-seq
alignment
updated 1 day ago by
Antonio R. Franco
★ 5.1k • written 6.0 years ago by
williamsbrian5064
▴ 510
14
votes
2
replies
7.0k
views
Trouble with Pilon installation
polishing
pilon
installation
updated 2 days ago by
Lissa Cruz Saavedra
• 0 • written 7.3 years ago by
Rox
★ 1.4k
7
votes
7
replies
5.9k
views
6 follow
How to know synonymous and non-synonymous SNPs from transcriptome sequencing
RNA-Seq
SNP
updated 1 day ago by
rohitsatyam102
▴ 850 • written 6.1 years ago by
smallfish
▴ 10
8
votes
5
replies
5.2k
views
ISCN annotation for SV/CN VCF files
SV
written 7 months ago by
a.beggs
▴ 60
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 3 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
2
votes
6
replies
3.6k
views
Treatment VS Control in Single Cell RNAseq analysis
Single Cell
RNAseq
DE Analysis
Treatment
Control
updated 1 day ago by
ATpoint
82k • written 3.9 years ago by
grayapply2009
▴ 280
0
votes
13
replies
3.5k
views
6 follow
Tax4Fun2 - error
tax4fun
silva
blast
updated 3 days ago by
drstalinantony28
• 0 • written 2.2 years ago by
ymj
▴ 10
0
votes
4
replies
2.7k
views
Lositan freezing when generating selection table
LOSITAN
updated 2 days ago by
evawillms183
• 0 • written 8.3 years ago by
andrepleao
• 0
1
vote
3
replies
2.5k
views
Problem to remove subset of patients with plink
plink
PED
updated 4 days ago by
chrchang523
10k • written 6.1 years ago by
Ginevra
▴ 10
2
votes
4
replies
2.4k
views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi
metagenomics
centrifuge
gi_taxid_nucl.dmp
taxonomy
updated 2 days ago by
ZhangYuanfeng
• 0 • written 2.4 years ago by
Frazier Baker
• 0
3
votes
3
replies
2.3k
views
How to make a TSS enrichment plot in ATAC-seq analysis?
ATAC
updated 2 days ago by
sp
• 0 • written 22 months ago by
Dan
▴ 180
2
votes
6
replies
1.8k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 15 hours ago by
Ram
43k • written 2.4 years ago by
SYOSY
▴ 10
2
votes
3
replies
950
views
Method to detect genome doubling
CNV
genome doubling
updated 5 days ago by
LauferVA
4.2k • written 4.5 years ago by
CY
▴ 750
1
vote
10
replies
923
views
Troubleshooting RNA-seq data with DNA contamination
RNA-seq
DESeq2
DNA-contamination
2 days ago by
DKA
▴ 40
2
votes
9
replies
836
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 3 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
8
votes
16
replies
829
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 23 hours ago by
bk11
★ 2.4k • written 7 weeks ago by
Sofia
• 0
0
votes
2
replies
803
views
AGeNT LocatIt
AGeNT
LocatIt
WES
UMI
duplicates
updated 2 days ago by
barslmn
★ 2.1k • written 2.8 years ago by
jhy
▴ 10
1
vote
8
replies
786
views
Adding CB tag to bam file
samtools
bam
updated 2 days ago by
Pierre Lindenbaum
161k • written 8 days ago by
Maria
• 0
3
votes
3
replies
719
views
KissDE and batch effect
kissDE
kissplice
5 days ago by
david.b.rombaut
▴ 10
2
votes
5
replies
688
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 6 days ago by
Jesse
▴ 740 • written 7 days ago by
Ann
★ 2.4k
3
votes
4
replies
664
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
4 days ago by
rahu
• 0
3
votes
6
replies
653
views
Ciriquant not configuring hisat2 indexed files
ciriquant
5 days ago by
Atul K.
• 0
5
votes
4
replies
633
views
How to compare the quality of assemblies
nextdenovo
assembly
hifiasm
pacbio
3 days ago by
kirillkirilenko
▴ 40
1
vote
5
replies
593
views
Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Series Library Preparation Set?
cell-barcode
MGI
DNBelab
single-cell
updated 3 days ago by
atowns21
• 0 • written 3 months ago by
benjamin.pyenson
• 0
0
votes
2
replies
584
views
Why cd-hit-est not work when sequence identity threshold<0.95?
genome
cluster
sequence
updated 2 days ago by
Dateline
• 0 • written 21 months ago by
JZX
• 0
4
votes
11
replies
559
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 3 days ago by
LauferVA
4.2k • written 4 days ago by
dominickd
• 0
8
votes
8
replies
557
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 2 days ago by
swbarnes2
14k • written 3 days ago by
Aaliya
▴ 10
3
votes
5
replies
551
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
5 days ago by
n_navy
• 0
2
votes
5
replies
526
views
MEGA Analysis Issue on High-Performance Computer
phylogenetic-analysis
MEGA
updated 6 days ago by
Mensur Dlakic
★ 27k • written 7 days ago by
Giorgio
• 0
0
votes
4
replies
524
views
how to find genes that are significantly expressed in brain cortex samples
rna-seq
gene-expression
updated 6 days ago by
ATpoint
82k • written 7 days ago by
rheab1230
▴ 140
4
votes
7
replies
514
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 9 hours ago by
dsull
★ 5.8k • written 4 days ago by
qudrat.nii
▴ 10
0
votes
11
replies
493
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
1 day ago by
atowns21
• 0
0
votes
3
replies
492
views
python file for coding potential calculator
cpc2.py
updated 4 days ago by
atharvakarkare14
▴ 10 • written 8 days ago by
Ashok
• 0
7
votes
6
replies
478
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
5 days ago by
analyst
▴ 30
2
votes
5
replies
455
views
Marking duplicates using UMIs
Deduplication
UMI
updated 5 days ago by
i.sudbery
19k • written 6 days ago by
Lipika
• 0
4
votes
8
replies
453
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 11 hours ago by
Alex Reynolds
35k • written 1 day ago by
J
▴ 10
3
votes
9
replies
440
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 3 days ago by
Istvan Albert
100k • written 4 days ago by
Κοσμάς
• 0
0
votes
6
replies
434
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 3 days ago by
gv
• 0 • written 26 days ago by
Srinka
▴ 20
0
votes
1
reply
430
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 1 day ago by
ginellegrenier
• 0 • written 4 months ago by
Clayton
• 0
4
votes
7
replies
429
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 2 days ago by
dsull
★ 5.8k • written 3 days ago by
VITALA
• 0
2
votes
2
replies
428
views
Using vg gamsort with naive sorting algorithm
gamsort
vg
updated 4 days ago by
Pierre Lindenbaum
161k • written 8 months ago by
AshleeThomson
▴ 80
1
vote
4
replies
425
views
GTF file for Rhinolophus sinicus
Rhinolophus-sinicus
GTF
updated 3 days ago by
atowns21
• 0 • written 5 months ago by
ara
• 0
1
vote
1
reply
419
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
updated 6 days ago by
Gordon Smyth
★ 7.0k • written 9 days ago by
Chris
▴ 260
0
votes
3
replies
373
views
How to identify gaps in a genome?
awk
seqkit
assembly
fasta
genome
updated 4 days ago by
colindaven
6.4k • written 12 days ago by
rj.rezwan
• 0
4
votes
2
replies
372
views
Missing protein (VEGF-A) in String db
VGFA
stringdb
updated 1 day ago by
shalespringer
• 0 • written 11 weeks ago by
Phenylananin
▴ 20
1
vote
5
replies
337
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
3 days ago by
anasjamshed
▴ 120
0
votes
8
replies
327
views
Error in cnetplot enrichplot package
R
updated 4 days ago by
Ram
43k • written 4 days ago by
Farhad
• 0
181 results • Page
1 of 4
Recent Votes
Calculate Allele balance by sample
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
Answer: Is there a tool that sorts gtf files?
Answer: gvcf joint calling
Answer: Heatmap and rna-seq
Answer: How to use limma to find differentially expressed genes in response to a continu
A: Blast Settings For Short Sequences
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rohitsatyam102
▴ 850
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▴ 10
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35k
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Recent Replies
Comment: different FeatureCounts output for the same data
by
Istvan Albert
100k
just to clarify, it is not that the program algorithm works differently but the meaning of the flags changed; before -p was sufficient to…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★ 1.7k
Thanks Ram. Using merge maxed out my ram on a large list, but your suggestion directed me to purrr::reduce which works great.
Comment: ScRNA data question
by
starswillfade
▴ 10
features <- SelectIntegrationFeatures(object.list = merged_dat) data.anchors <- FindIntegrationAnchors(object.list = merged_dat, …
Comment: gvcf joint calling
by
zihanss
• 0
Thank you for your help! I really appreciate it! And you know, the merged WES gVCF files still have "NA" loci. For such cases, I am quite …
Comment: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Guille
• 0
Thank you for your answer! The MDS plot seems to group most treatment groups close-by, so I'm starting to suspect the effect of treatment …
Answer: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
by
Mensur Dlakic
★ 27k
They say that we remember better things we figured out on our own, rather than things we were taught. You were literally two clicks away fr…
Comment: Heatmap and rna-seq
by
dsull
★ 5.8k
https://maayanlab.cloud/clustergrammer/ It's a web-based tool (can also be a python jupyter widget) that I find useful for exploring a pat…
Answer: Limma Analysis Agilent Microarray Data (GPL1708)
by
Gordon Smyth
★ 7.0k
There is an Agilent array case study in the limma User's Guide, which you might find helpful and somewhat simpler. I don't recommend col…
Answer: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Gordon Smyth
★ 7.0k
The comparison you've done seems exactly right to test whether treatment 2 modifies or enhances the effect of treatment 1. Have you exam…
Answer: How to use limma to find differentially expressed genes in response to a continu
by
Gordon Smyth
★ 7.0k
There's no need for form a contrast because the score variable is already in the model. A continuous variable is its own contrast. Just run…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Oh wow, bedops closest-features, how did I overlook that!! I didn't know about it; thank you! Your awk script is beautiful. This worked per…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Thank you Pierre! I'll try this out with my full data set.
Answer: DNA methylation preprocessing
by
cao510927
▴ 30
I happend to see one paper doing the similar thing, share it with you guys, "A systematic evaluation of normalization methods and probe rep…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Alex Reynolds
35k
I think Pierre's answer might be correct for your test input, but it doesn't account for the start position of the reference element and so…
Comment: Highest variable features in single cell data
by
bk11
★ 2.4k
You need to be clear about how `Seurat` defines highly variable genes here. Highly variable genes are the genes that have very high express…
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