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121,937 results • Page 130 of 2439
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2
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1.0k
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Why are gene models from JGI non-sense when using AGAT to to extract proteins?
translation models gif gene cgi
18 months ago by O.rka ▴ 750
0
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0
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511
views
details about PCA in plink 2.0: eigenvectors, PCs, scores
gwas plink pca
18 months ago by kaybelter • 0
1
vote
2
replies
721
views
Database for non cancer cell lines
cell-line L1000 rna-seq cancer-database LINCS
18 months ago by ANSARAHMAD • 0
2
votes
2
replies
936
views
Convert to ENTREZGENE
maize ENTREZ
18 months ago by san96 ▴ 190
0
votes
1
reply
932
views
How to calculate depth of coverage and applying read depth filter
Depth-of-Coverage CRAM WGS
updated 18 months ago by 45k • written 18 months ago by • 0
1
vote
3
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1.2k
views
BioGRID REST - How to retrieve all the protein interactions?
BioGRID Python REST
updated 18 months ago by 166k • written 18 months ago by • 0
4
votes
4
replies
1.3k
views
Problem generating paired end reads when converting Cell Ranger's BAM result to FASTQ
fastq bam
18 months ago by ntuzov • 0
0
votes
1
reply
599
views
Finding chimeric reads for CRISPR edited mouse cells from RNA-Seq data
RNA-seq crispr
updated 18 months ago by ▴ 740 • written 18 months ago by • 0
4
votes
7
replies
1.8k
views
Can any JBrowse2 tracks show multiple colors for reads at different nucleotide positions?
JBrowse JBrowse2
updated 18 months ago by ★ 4.3k • written 18 months ago by ▴ 160
0
votes
1
reply
854
views
Tissue specific mRNA expression dataset in human and mouse
tissue-specificity
updated 18 months ago by 45k • written 18 months ago by ★ 9.6k
2
votes
3
replies
2.7k
views
Mapping ortholog human and mouse transcript
biomart
18 months ago by 1769mkc ★ 1.3k
0
votes
3
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902
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How do I get the gene annotations as a text file from a genbank file?
sequence-annotation genbank
updated 18 months ago by 45k • written 18 months ago by • 0
3
votes
2
replies
1.4k
views
News: Announcement: Research Topic "Mechanistic, Machine Learning and Hybrid Models of the 'Other' Endocrine Regulatory Systems in Health and Disease", Vol…
mathematical-modelling endocrinology ML systems-biology machine-learning
18 months ago by Johannes W. Dietrich • 0
0
votes
1
reply
631
views
Alignment with new reference
alignment
updated 18 months ago by 154k • written 18 months ago by ▴ 180
0
votes
1
reply
628
views
Bowtie2 alignment rate
galaxy Bowtie alignment-rate
updated 18 months ago by • 0 • written 18 months ago by • 0
2
votes
1
reply
669
views
Is RNAseq data from a monoclonal cell line technically considered scRNAseq?
scRNA RNAseq
updated 18 months ago by ▴ 110 • written 18 months ago by • 0
1
vote
0
replies
529
views
Synthetic lethality and cancer database
interaction lethal synthetic
18 months ago by Shicheng Guo ★ 9.6k
4
votes
9
replies
2.9k
views
Error in openning FastQC
fastqc igv
updated 18 months ago by 154k • written 2.1 years ago by • 0
0
votes
2
replies
1.2k
views
vcf file: number of samples does not match number of columns
vcf bcftools
18 months ago by ekirsch • 0
0
votes
4
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869
views
Is it reasonable to discard reads that show variation of quality across its length?
Trimming
18 months ago by BRENO • 0
0
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5
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1.3k
views
Error running local blastn in R using system2
blastn NCBI R
18 months ago by Harrison • 0
16
votes
10
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2.5k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 18 months ago by 5.3k • written 18 months ago by ★ 2.4k
0
votes
0
replies
503
views
News: Online course: Reproducibility in Bioinformatics
Singularity Version-Control Docker Containers
updated 18 months ago by 45k • written 18 months ago by ★ 2.6k
0
votes
0
replies
402
views
News: Online course: Analysis of Prokaryotic Pangenomes (Last call)
Prokaryotic Bacterial-Genomics Pangenomics
updated 18 months ago by 45k • written 18 months ago by ★ 2.6k
0
votes
1
reply
648
views
Annotation of Complex genomes using NCBI Genome Workbench
Genome-Workbench MAKER
updated 18 months ago by • 0 • written 18 months ago by • 0
0
votes
1
reply
704
views
Looking for RPIP Illumina kit probe sequences
Sequences Illumina
updated 18 months ago by 154k • written 18 months ago by • 0
0
votes
9
replies
1.6k
views
Low number of both surviving reads after trimming
trimmomatic trimming
18 months ago by Jay • 0
0
votes
2
replies
717
views
ONT direct RNA sequencing
DRS ONT
updated 18 months ago by 45k • written 18 months ago by • 0
2
votes
4
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914
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP CPU transcriptomics
18 months ago by Mohamed Abderrahmane ▴ 20
3
votes
2
replies
777
views
Gene reads all zero for each sample
R salmon DE DESeq2
18 months ago by DYLAN NICO • 0
3
votes
2
replies
847
views
Can I have multiple active GDC download tokens?
TCGA
updated 18 months ago by ★ 1.3k • written 18 months ago by • 0
1
vote
0
replies
431
views
Contradictions in GSEA results
GSEA Enrichment-analysis PEA
updated 18 months ago by 45k • written 18 months ago by ▴ 30
0
votes
0
replies
376
views
Illumina Dna Prep kit for sequencing phage linear genomes
tagmentation transposons phagetermini sequencing
18 months ago by alenew.am ▴ 10
0
votes
3
replies
2.8k
views
FindAllMarkers not working (Error (data layers are not joined. Please run JoinLayersWarning: When testing 1 versus all)
FindAllMarkers seurat re-clustering
18 months ago by Nitin • 0
0
votes
1
reply
483
views
(sc)RNA-seq dataset for these cell lines: HEK293, HeLa, PC3 and U2OS
scRNA-seq
updated 18 months ago by 45k • written 18 months ago by • 0
0
votes
1
reply
542
views
Normalization strategies for comparing mapped reads across samples in RNA-seq
normalization rnaseq
updated 18 months ago by 45k • written 18 months ago by ▴ 690
0
votes
0
replies
408
views
How to change alternate conformation occupancy ?
Bio.PDB.Atom Bio.PDB biopython
updated 18 months ago by 45k • written 18 months ago by • 0
1
vote
2
replies
734
views
Issue with making local BLAST database
R mitochodrial diatoms blast ncbi
updated 18 months ago by 45k • written 18 months ago by ▴ 210
1
vote
1
reply
837
views
diffbind : killed dba.count(DBsample, bParallel=FALSE)
diffbind r atacseq
18 months ago by Pierre Lindenbaum 166k
1
vote
2
replies
888
views
Flow Cytometry Data Analysis by Seurat
Seurat
updated 18 months ago by 45k • written 18 months ago by • 0
3
votes
12
replies
1.9k
views
struggle to get fasta files from ucsc goldenPath
ucsc getfasta fasta
18 months ago by Lila M ★ 1.3k
3
votes
3
replies
1.1k
views
Volcano Plot Output Inquiry: Graphs Facing Down
rstudio volcano-plot
18 months ago by Pumla • 0
0
votes
2
replies
753
views
Merging featureCounts output for different species
featureCounts RNA-seq
updated 7 months ago by ★ 4.4k • written 18 months ago by • 0
3
votes
2
replies
986
views
Interproscan taking so much time
slurm interproscan transcriptomics
updated 18 months ago by ★ 3.1k • written 18 months ago by ▴ 20
0
votes
0
replies
527
views
Combine rename and rmdup in SeqKit to remove duplicate sequences and append N in header? Sort by occurence?
seqkit fasta
18 months ago by Broccoli • 0
0
votes
0
replies
357
views
Is there a good tool/reference for PBP and porin analysis
PBP porin
18 months ago by blur ▴ 280
0
votes
0
replies
379
views
meta-analysis on RNA seq AND microarray results
microarray RNA
18 months ago by liuyibin2005 • 0
59
votes
30
replies
16k
views
13 follow
Tutorial: How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation meta how-to
updated 18 months ago by 103k • written 9.6 years ago by 45k
0
votes
0
replies
354
views
SPOTlight deconvolution logFC, logFC cohen or mean.AUC
spatial-transcriptomics SPOTlight logFC deconvolution
updated 18 months ago by 45k • written 18 months ago by • 0
0
votes
5
replies
878
views
calculate mismatch rate from VCF file
mismatch-rate VCF
updated 18 months ago by 45k • written 18 months ago by ▴ 10
121,937 results • Page 130 of 2439
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Answer: Linear regression vs DESeq2 models for DEG analysis
A: What'S The Difference Between Cds And Orf?
A: What'S The Difference Between Cds And Orf?
A: What'S The Difference Between Cds And Orf?
A: What'S The Difference Between Cds And Orf?
Answer: Linear regression vs DESeq2 models for DEG analysis
Answer: Linear regression vs DESeq2 models for DEG analysis
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Comment: Linear regression vs DESeq2 models for DEG analysis by swbarnes2 15k
It is not clear to me that you can meaningfully correct for 5 different batches with two samples a piece.
Comment: Does this adapter content from fastqc to warrant trimming prior to alignment? by GenoMax 154k
> I am not sure if there would be primer dimer sequences I should have said adapter dimers above. Correction made.
Comment: Help understanding this per base sequence content failure fastqc plot by lieven.sterck 15k
that 151st base added is a base to ignore (it's a kind of random addition) ... if I recall correctly it's a consequence of the technology. …
Comment: Linear regression vs DESeq2 models for DEG analysis by FioG • 0
@swbarnes2 Thank you for your response! I am new to RNA seq analysis and was originally recommended to use the LM method following cqn and …
Comment: Does this adapter content from fastqc to warrant trimming prior to alignment? by curious ▴ 890
Thank you, the adapter sequences are specified as CTGTCTCTTATACACATCT+ATGTGTATAAGAGACA is the SampleSheet.csv file from the sequencing run.…
Comment: how to explain the sample size discrepancy in UCSC Xena by nuorain ▴ 40
Thank you very much.
Answer: how to explain the sample size discrepancy in UCSC Xena by i.sudbery 22k
Because there wasn't RNA-seq analysis performed for every sample in TCGA?
Answer: Linear regression vs DESeq2 models for DEG analysis by i.sudbery 22k
There are two main problems with using linear models for read-count based analyses. Firstly, linear models assume a normal distribution…
Comment: real time PCR data analysis by ATpoint 89k
I cannot guide on GraphPad. It's commercial software so better contact their support.
Answer: Linear regression vs DESeq2 models for DEG analysis by swbarnes2 15k
DE genes in bulk RNASeq is what DESeq2 was made for, so why wouldn't you use it? RPKM is not an appropriate normalization method here. M…
Comment: What happened to biomart.org by Jeremy Leipzig 23k
same with geuvadis.org
Comment: real time PCR data analysis by Gordon Smyth ★ 8.3k
Are you doing qPCR for one gene or for several genes?
Comment: protein interaction prediction by Fatemeh ▴ 50
Thanks very much for your help. I need to try it.
Comment: real time PCR data analysis by Fatemeh ▴ 50
Thanks a lot for the link. The problem is that with this repeated measures, if you put the data from different donors in different rows, fo…
Answer: What happened to biomart.org by GenoMax 154k
Looks like EBI let the domain registration expire. The domain was picked up by someone else and turned into the site OP mentions. There use…
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