Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
121,956 results • Page
164 of 2440
Sort: Rank
Rank
Views
Votes
Replies
2
votes
4
replies
1.4k
views
Sorted bam files have zero content
bam
picard
DNA-seq
sorted
updated 20 months ago by
Michael
56k • written 21 months ago by
mgranada3
▴ 60
2
votes
1
reply
1.6k
views
Forum:
Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist
transcriptomics
nanostring
xenium
visium
21 months ago by
LauferVA
4.8k
9
votes
12
replies
3.0k
views
BUSCO duplicated
BUSCO
Trinity
updated 20 months ago by
GenoMax
154k • written 21 months ago by
san96
▴ 190
2
votes
2
replies
787
views
0% Alignment of Reads to Herpesvirus Genome
alignment
hisat2
bwa
rnaseq
virus
21 months ago by
vajefferson77
• 0
4
votes
1
reply
1.3k
views
RNA-seq DE analysis: repeated measures design (options available, mixed models?)
repeated
RNA-seq
updated 21 months ago by
ATpoint
89k • written 21 months ago by
bio.infanatics
▴ 10
2
votes
1
reply
1.4k
views
Input for Deseq2 for downstream analysis
Rna-seq
updated 21 months ago by
Ram
45k • written 21 months ago by
وفاء
• 0
0
votes
1
reply
767
views
News:
Reminder: NCBI ClinVar XML update to accommodate somatic variants coming Jan 29, 2024
SNP
XML
ClinVar
NCBI
21 months ago by
PeterC_NCBI
▴ 520
1
vote
1
reply
630
views
Extract Domain Architectures from Pfam / Interpro
interpro
domain-architecture
pfam
updated 21 months ago by
bk11
★ 3.1k • written 21 months ago by
Jimpix
▴ 10
0
votes
4
replies
1.0k
views
Cyberduck installation issues
NGS
cyberduck
updated 21 months ago by
Ram
45k • written 21 months ago by
Panagiotis
• 0
0
votes
0
replies
447
views
GSEA for ChIP/ATAC-seq
ATAC-seq
GSEA
21 months ago by
Orange
▴ 30
4
votes
10
replies
2.9k
views
6 follow
Deciding if a study used Bulk or single-cell RNA sequencing using the GEO data
GEO
RNA-seq
updated 21 months ago by
Ram
45k • written 21 months ago by
AlexStar
▴ 200
0
votes
1
reply
610
views
insilico prediction of disulfide bonds in protein
disulfide-bond
updated 21 months ago by
CTLong
▴ 140 • written 21 months ago by
sindy_roby
• 0
0
votes
1
reply
722
views
Extracting LOF variants and incidence from GnomAD for a list of genes
Gnomad
LOF
genes
updated 21 months ago by
LauferVA
4.8k • written 21 months ago by
amy__
▴ 250
2
votes
1
reply
697
views
Statistical analysisi in DESeq2 with different condition
statistics
DESeq2
updated 21 months ago by
Shred
★ 1.6k • written 21 months ago by
michelafrancesconi9
▴ 20
1
vote
1
reply
578
views
Mgnify download
Comparative-analysis
updated 21 months ago by
Ram
45k • written 21 months ago by
bala
• 0
0
votes
2
replies
582
views
Issue with chromstaR ATACseq, unable to find genome?
ATAC-seq
updated 21 months ago by
Ram
45k • written 21 months ago by
Ronin
▴ 10
0
votes
0
replies
439
views
How to tackle with LRG symbols from peak annotation by ChIPpeakAnno?
ChIPpeakAnno
Bioconductor
ChIP-seq
20 months ago by
namck
• 0
4
votes
0
replies
604
views
News:
Transform Your Research with Our NGS Data Analysis Online Course!
DNAseq
NGS
Biologists
OnlineCourse
RNAseq
21 months ago by
David Langenberger
11k
0
votes
12
replies
2.8k
views
BWA Index Referencing Failed. Possible Reason and Solutions?
BWA
updated 20 months ago by
a.alnawfal.1992
▴ 360 • written 21 months ago by
Tundup
▴ 10
2
votes
1
reply
771
views
How to interpret Per sequence GC content in FastQC for ChIP-seq data?
FastQC
ChIP-seq
updated 21 months ago by
ATpoint
89k • written 21 months ago by
Logan
• 0
0
votes
0
replies
455
views
Long cDNA reads identification with Pinfish
long-reads
cDNA
nanopore
pinfish
updated 18 months ago by
Ram
45k • written 21 months ago by
wqqgenetics
• 0
0
votes
3
replies
1.2k
views
CPTAC RNA seq
raw
CPTAC
transcriptomics
count
20 months ago by
Rob
▴ 180
0
votes
0
replies
481
views
Perform mean imputation
Mean
Imputation
21 months ago by
Patrick
▴ 30
0
votes
2
replies
2.0k
views
problem with importing mtx file in scanpy
scanpy
scRNAseq
21 months ago by
QX
▴ 80
4
votes
8
replies
1.9k
views
DESeq2 heatmap with low baseMean values
DESeq2
20 months ago by
Ahmed
▴ 10
0
votes
0
replies
485
views
Loop and TAD calling from HiC reads using HOMER
HiC
HOMER
updated 21 months ago by
Ram
45k • written 21 months ago by
arsala521
▴ 60
0
votes
1
reply
625
views
Compute ld and genomic distance using clump function in plink
plink
updated 21 months ago by
bk11
★ 3.1k • written 21 months ago by
MAPK2
▴ 50
1
vote
1
reply
519
views
Transcriptome data analysis
transcriptome
updated 21 months ago by
dthorbur
★ 3.1k • written 21 months ago by
nesanemashudu
• 0
9
votes
7
replies
3.3k
views
Trying to wrap my head around Illumina paired end sequencing
paired-end
illumina
sequencing
updated 20 months ago by
Istvan Albert
103k • written 21 months ago by
ScottDansk
▴ 10
6
votes
6
replies
1.7k
views
Differential expression analysis in a short gene list
differential-expression
DESeq2
RNA-seq
21 months ago by
yixinzeng
• 0
0
votes
2
replies
1.2k
views
How to launch MAUVE on Ubuntu 22?
mauve
ubuntu
aligner
updated 21 months ago by
brian.fristensky
▴ 460 • written 21 months ago by
marongiu.luigi
▴ 760
0
votes
0
replies
394
views
How to identify structural variants (SV) breakpoints using alignment PAF file?
SV
minimap2
genome
alignment
21 months ago by
BioinfoBee
• 0
0
votes
3
replies
2.4k
views
Generate VCF from different .bam files with different chromosome names
bcftools
mpileup
vcf
updated 21 months ago by
Ram
45k • written 5.5 years ago by
evoclive
• 0
2
votes
0
replies
891
views
Herald:
The Biostar Herald for Monday, January 08, 2024
herald
21 months ago by
Biostar
3.6k
6
votes
7
replies
1.7k
views
Finding EntreZ IDs for refseq IDs
RNA-SEQ
20 months ago by
Pegasus
▴ 130
4
votes
9
replies
5.7k
views
9 follow
GEM index fatal error
GEM
gem-indexer
updated 21 months ago by
Ram
45k • written 7.4 years ago by
lyz10302012
▴ 490
4
votes
8
replies
3.5k
views
6 follow
phylogeny
phylogenetic-tree
diversity
21 months ago by
1509636876
▴ 10
0
votes
6
replies
1.3k
views
xCell different between R and website
xcell
21 months ago by
michelafrancesconi9
▴ 20
4
votes
4
replies
1.4k
views
Question about identification of DEGs in paired sample using edgeR
edgeR
DEG
21 months ago by
tujuchuanli
▴ 130
1
vote
1
reply
532
views
how can get NEB_barcode16.fasta?
Transcriptome
updated 21 months ago by
Ram
45k • written 21 months ago by
1174530821
• 0
0
votes
0
replies
388
views
Why does coverageBed give me 13 columns even though i use -count option
bedtools
coverage
coverageBed
21 months ago by
SangHyun
• 0
2
votes
7
replies
3.6k
views
How to retrieve the corresponding genome assembly accession ID from a nucleotide accession ID using NCBI eUtility command
eDirect
NCBI
eUtility
Entrez
Assembly
21 months ago by
Mani
• 0
0
votes
2
replies
739
views
GeneIDs to TAIR ids
TAIR
20 months ago by
Kutti
• 0
0
votes
0
replies
410
views
extracthifi outputs a file with a size larger than the input file
pbtk
PacBio
extracthifi
HiFi
21 months ago by
yh1126
• 0
3
votes
14
replies
3.8k
views
7 follow
Some lines are not well organized in BED file, how to correct them ? Thanks a lot !
BED
updated 20 months ago by
Alex Reynolds
36k • written 21 months ago by
Tom
• 0
1
vote
2
replies
785
views
dna data for HEK-293 cells
HEK-293
DNA
updated 21 months ago by
Ram
45k • written 21 months ago by
sona
• 0
0
votes
3
replies
1.1k
views
Remove Uncharacterized chromosomes before alignment in chipseq
fasta
Bowtie2
updated 21 months ago by
ATpoint
89k • written 21 months ago by
Chironex
▴ 50
0
votes
1
reply
572
views
R studio help - ROH
R
ROH
updated 14 months ago by
mmisalem
• 0 • written 21 months ago by
u19010843
• 0
2
votes
5
replies
1.4k
views
RNA-seq deconvolution algorithm that takes into account weight of marker gene
RNA-seq
updated 21 months ago by
Shred
★ 1.6k • written 21 months ago by
CTLong
▴ 140
0
votes
5
replies
1.4k
views
how to start DEG analysis after featurecounts
RNAseq
DEG
21 months ago by
kayah
▴ 20
121,956 results • Page
164 of 2440
Recent Votes
Comment: limmaFit error when comparing TCGA RNA-Seq data
A: The Biostar Handbook. A bioinformatics e-book for beginners.
Modernized RNA-MuTect pipeline for tumor-only RNA-seq somatic variant calling
Dictys refuses to use CUDA supported pytorch during installation
Answer: Dictys refuses to use CUDA supported pytorch during installation
Comment: Looking to learn practical bioinformatics
Answer: MAPQ algorithms of Giraffe
Recent Locations •
All
United States,
8 minutes ago
Canada,
18 minutes ago
France,
27 minutes ago
United States,
33 minutes ago
United States,
38 minutes ago
United States,
55 minutes ago
Norway,
1 hour ago
Recent Awards •
All
Popular Question
to
sacha
★ 2.5k
Popular Question
to
Guilherme
• 0
Commentator
to
GenoMax
154k
Popular Question
to
Ibrahim Tanyalcin
★ 1.2k
Teacher
to
colindaven
8.0k
Popular Question
to
m90
▴ 30
Popular Question
to
PolenP
• 0
Recent Replies
Comment: limmaFit error when comparing TCGA RNA-Seq data
by
vernonlim98
• 0
Thanks for the advice. Am still new to RNA seq and R programming in general, and will just do the analysis by myself.
Answer: The Biostar Handbook. A bioinformatics e-book for beginners.
by
Istvan Albert
103k
Update as of **August 2025**: The book now includes a new online course: [**FastTrack Bioinformatics 2025**][course] at no extra cost. Bio…
Comment: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
by
GenoMax
154k
Likely because the SRA programs are doing database look ups as they retrieve the data where as with `curl` you are simply getting pre-exist…
Comment: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
by
Guilherme
• 0
That's a great tool! I wonder why downloading through curl via SRA's ftp is SO MUCH faster than through sra toolkit programs (fastq-dump or…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
GenoMax
154k
> they should align with common sequence at some ends, making one longer consensus sequence. You don't want to simulate reads like this.…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
cmdcolin
★ 4.3k
I believe the pairs will only have common sequence (e.g. the pairs will "overlap") if the insert size is small. the wgsim program has a fla…
Answer: X chromosome male and female imputation
by
barslmn
★ 2.4k
I think it's better to use the original 900 variants. If you have genotype information, you can get the fraction of heterozygous variants t…
Comment: Slam-Seq Conversion Rate per Read
by
michael.ante
★ 4.0k
Try to switch to percentage view of this graph. Stacked bar charts with different heights can sometimes be misleading.
Comment: Functional prediction for proteins of bacteria.
by
m90
▴ 30
Biochemical changes
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
PolenP
• 0
Sorry about that. It's when I align the paired reads together like the left and righ, they should align with common sequence at some ends, …
Answer: Dictys refuses to use CUDA supported pytorch during installation
by
zwjiang
▴ 30
I fixed my problem by ``` conda create -y -p /project/spott/zwjiang/conda_envs/dictys_gpu -c conda-forge python=3.9 mamba conda activat…
Comment: HIV : gtf or gff annotation
by
Ales
▴ 50
Thank you for your response! We have just posted the full preprint to BioRxiv (https://www.biorxiv.org/content/10.1101/2025.09.24.675449v1)…
Comment: Does this adapter content from fastqc to warrant trimming prior to alignment?
by
curious
▴ 890
Just adding some detail here that may help draw some connections with other posts, I did a bunch of sleuthing with selectively trimming dif…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
cmdcolin
★ 4.3k
It's a little unclear what you mean by "I was able to use wgsim, but when I tried aligning the paired-reads, it's not aligning together."
Comment: using packages with conda
by
ATpoint
89k
Just to be sure: You ssh into a remote server, correct? Die you setup the conda environment on your local machine or the remote? Must be th…
Traffic: 3699 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6