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121,958 results • Page
178 of 2440
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
678
views
Population study based on metaT and MetaG
metagenome
metatranscriptome
study
population
updated 22 months ago by
GenoMax
154k • written 22 months ago by
PierreGT
• 0
9
votes
7
replies
2.2k
views
p-value combination methods
rnaseq
NB-GLM
p-value
meta-Analysis
updated 22 months ago by
Istvan Albert
103k • written 22 months ago by
sehriban.buyukkilic
▴ 10
0
votes
0
replies
576
views
sequencing deep mutational scanning library
mutagenesis
illumina
long-read
sequencing
22 months ago by
reaset41
• 0
0
votes
4
replies
1.4k
views
Making multiple box plots with relative abundance in R
R
Boxplots
22 months ago by
ohtang7
▴ 40
1
vote
1
reply
687
views
Two identical reads in my bam files but with a different in CIGAR are mapped in a different location in IGV
pysam
22 months ago by
ManuelDB
▴ 110
0
votes
1
reply
590
views
GMT file from eggnog annotation
gmt
annotation
Eggnog
updated 10 months ago by
leandrovb88
• 0 • written 22 months ago by
jorge.cortes.miranda
• 0
4
votes
7
replies
1.3k
views
regarding blast result interpretation
blast
updated 22 months ago by
dthorbur
★ 3.1k • written 22 months ago by
Raman
• 0
2
votes
3
replies
1.1k
views
Blastn DB issue
cgmlst
blastn
updated 22 months ago by
Philipp Bayer
8.9k • written 22 months ago by
hakhasan
• 0
1
vote
2
replies
1.0k
views
How can I amend the output of a DIAMOND python script?
Python
Diamond
COG_analysis
22 months ago by
eam-hmc
• 0
1
vote
2
replies
3.6k
views
Problem in installing 'magick' R package
R
installation
package
magick
updated 22 months ago by
DBScan
▴ 530 • written 22 months ago by
applepie
▴ 10
1
vote
1
reply
745
views
FVA on Sybil
R
Sybil
metabolic
fba
fva
updated 22 months ago by
Thomas
▴ 10 • written 3.7 years ago by
isMM
• 0
0
votes
3
replies
1.0k
views
Viral genes not showing up in combined mouse+virus alignment
RNAseq
22 months ago by
cook.675
▴ 250
2
votes
2
replies
1.1k
views
How to create a mutation frequency comparison plot?
R
data-visualization
plot
updated 22 months ago by
rpolicastro
13k • written 22 months ago by
saipra003
▴ 20
3
votes
5
replies
2.0k
views
CHIPSEQ : Cut AND Run , DiffBind Parameters
Cut-Run
DiffBind
ChIP-seq
updated 22 months ago by
Rory Stark
★ 2.1k • written 22 months ago by
RD
▴ 30
0
votes
0
replies
476
views
MUSCLE Protein Alignment of Distantly Related Proteins
muscle
protein
alignment
22 months ago by
Richard Fontaine
• 0
4
votes
6
replies
1.9k
views
bwa mem hangs after a few thousand reads
bwa
alignment
variant-calling
bwa-mem
updated 18 months ago by
Ram
45k • written 22 months ago by
cee28
▴ 30
2
votes
1
reply
1.3k
views
How to get unaligned reads and aligned reads into separate files from SAM/BAM?
bam
sam
reads
fastq
updated 22 months ago by
rpolicastro
13k • written 22 months ago by
O.rka
▴ 750
2
votes
2
replies
1.6k
views
Using ggplot2 to make barplots of RNASeq data - maintaining sample metadata when pivoting from wide to long format
rnaseq
pivot_longer
ggplot2
updated 22 months ago by
cmdcolin
★ 4.3k • written 22 months ago by
Dylan C-C
• 0
3
votes
4
replies
1.8k
views
Plotting a pvalue threshold for an FDR corrected pvalue <.05 when thats not present in R
FDR
volcano
plotting
R
plot
updated 22 months ago by
Nitin Narwade
★ 1.7k • written 22 months ago by
RNAseqer
▴ 280
0
votes
1
reply
817
views
bam or VCF files from GSE75010
bam
file
or
VCF
updated 22 months ago by
ATpoint
89k • written 22 months ago by
ahonya
• 0
0
votes
0
replies
550
views
Bcftools consensus when reference is a deletion
consensus
vcf
22 months ago by
selplat21
▴ 20
2
votes
4
replies
1.3k
views
How should I run ssgsea analysis ?
functional
TPM
enrichment
gsea
ssgsea
updated 22 months ago by
Ram
45k • written 22 months ago by
DareDevil
★ 4.4k
0
votes
5
replies
1.5k
views
How to deeply sequence long inserts
Long-read
phage
NGS
updated 18 months ago by
Ram
45k • written 22 months ago by
Ryan
• 0
0
votes
2
replies
1.2k
views
VCF conservation into Treemix
Treemix
Plink
VCF
21 months ago by
reza
▴ 300
1
vote
2
replies
1.2k
views
Comparing 3 Data Sets using DeSeq and Heatmaps
DESeq2
Normalization
updated 21 months ago by
Ram
45k • written 22 months ago by
Bio User
• 0
0
votes
0
replies
530
views
scVelo cell transitions from marker gene expressing cells
velocity
scvelo
RNA
22 months ago by
rbronste
▴ 420
3
votes
2
replies
1.1k
views
Moving AF tag to new column in a VCF file
mutect2
vcf
22 months ago by
Christian
▴ 40
1
vote
5
replies
1.3k
views
How can I map coordinates between two strains of yeast?
coordinates
yeast
genome
mapping
fasta
22 months ago by
Jimmy
▴ 30
0
votes
1
reply
689
views
The difference blastn output when using subject and db options
genome
blastn
mite
annotation
tracker
updated 22 months ago by
GenoMax
154k • written 22 months ago by
praasu
▴ 40
1
vote
8
replies
3.0k
views
featureCounts results: low rate of 'Successfully assigned alignments'
FeatureCounts
HISAT2
RNA-seq
updated 21 months ago by
Yang
• 0 • written 22 months ago by
Hamtaro
▴ 60
0
votes
1
reply
751
views
Python function to export the annotated records to genbank file using python and biopython
biopython
python
bioinformatic
programming
updated 22 months ago by
barslmn
★ 2.4k • written 22 months ago by
Saurabh
• 0
1
vote
6
replies
1.3k
views
Discrepancy in total number of bases in trimmed read1 and read2 files after BBDuk
bbduk
22 months ago by
CTLong
▴ 140
2
votes
1
reply
932
views
unicycler error : [Errno 13] Permission denied:
unicycler
updated 22 months ago by
barslmn
★ 2.4k • written 22 months ago by
jiyoung
▴ 20
2
votes
4
replies
1.5k
views
Protein Database
protein
course
database
updated 22 months ago by
Elisabeth Gasteiger
★ 2.4k • written 22 months ago by
Mery
• 0
0
votes
0
replies
493
views
Does breadth of coverage signify expression?
Coverage
breadth
Bedtools
Expression
count
22 months ago by
Smriti
▴ 40
0
votes
6
replies
1.7k
views
I made an error when using metawrap to binning
metagenome
binning
metawrap
22 months ago by
alice005201314
• 0
1
vote
4
replies
1.1k
views
Bowtie mapping for single_end read
bowtie
22 months ago by
Rita
• 0
0
votes
4
replies
831
views
MRI VBM issue
MRI
VBM
ROI
Rhesus
22 months ago by
Ethan Lee
• 0
5
votes
3
replies
3.3k
views
SPAdes error: finished abnormally, OS return value: 21
SPAdes
updated 22 months ago by
Brian Bushnell
20k • written 22 months ago by
jiyoung
▴ 20
0
votes
5
replies
2.0k
views
Lack of correspondance of GFA node IDs to giraffe/call node IDs
giraffe
pggb
vg
updated 22 months ago by
Wenhai
• 0 • written 23 months ago by
javierbelmor
• 0
0
votes
0
replies
469
views
missing region in the process of annotation
annotatr
minfi
22 months ago by
Aki
▴ 20
0
votes
3
replies
6.9k
views
pheatmap lengends are being cutoff
pheatmap
legends
rstudio
heatmaps
updated 22 months ago by
Chris S.
▴ 340 • written 4.5 years ago by
enh
• 0
0
votes
2
replies
1.1k
views
Help doing differential expression analysis -experimental design and gProfiler TF interpretation-
gProfiler
mRNA
Differential-expression
TCGA
updated 22 months ago by
rajabolacuan
• 0 • written 22 months ago by
Camilo Andres
▴ 40
0
votes
1
reply
1.2k
views
Diferences between TCGAbiolinks and cBioportal
R
TCGAbiolinks
cBioportal
updated 22 months ago by
Zhenyu Zhang
★ 1.3k • written 22 months ago by
jomagrax
▴ 40
1
vote
0
replies
578
views
Visualize and explore eventalign data against reference
igv
minimap
nanopolish
nanopore
ngs
22 months ago by
noodle
▴ 650
0
votes
6
replies
1.5k
views
Best practices for differential expression analysis with low-yield Nanopore/ONT direct cDNA data?
differential-expression
RNA-Seq
ONT
Nanopore
updated 18 months ago by
Ram
45k • written 22 months ago by
tw_140
• 0
3
votes
2
replies
1.7k
views
Running STAR on fastq file generated from a RNA-seq experiment
STAR
RNAseq
fastq
files
updated 22 months ago by
Darked89
4.7k • written 22 months ago by
achanda
• 0
3
votes
3
replies
1.9k
views
DEseq2 input
DEseq2
updated 22 months ago by
ATpoint
89k • written 22 months ago by
r.shiasi3897
▴ 10
0
votes
2
replies
833
views
Combine GEO ids
NCBI
Geo
updated 19 months ago by
Ram
45k • written 22 months ago by
anasjamshed
▴ 140
2
votes
2
replies
1.6k
views
Best practices for unstranded sequences in featureCounts
featurecounts
overlapping
unstranded
22 months ago by
Enrique
• 0
121,958 results • Page
178 of 2440
Recent Votes
sra-explorer : find SRA and FastQ download URLs in a couple of clicks
Comment: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
Answer: Tools to simulate Illumina short read sequences and ONT long reads with a refere
Answer: Gene Ontology visualization
A: For CreateSeuratObject, Where Do the Values for min.cells and min.features come
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Answer: With 10x Visum HD : How to map the barcode id to the barcode sequence ?
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Popular Question
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cmdcolin
★ 4.3k
Popular Question
to
yura.grabovska
▴ 820
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Ashwin
▴ 70
Popular Question
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sacha
★ 2.5k
Scholar
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zwjiang
▴ 30
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Guilherme
• 0
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GenoMax
154k
Recent Replies
Comment: Errors with RSEM/bowtie2
by
ofarinas621
• 0
Yes, I already made the transcriptome with these tools, and the CD-HIT-EST fasta file is the transcript file that I am using for RSEM. Then…
Comment: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
by
Guilherme
• 0
Oh, ok. It seems like NCBI stores the files in SRA format (thus needing conversion and database lookups by sra toolkit tools) and EBI store…
Comment: help with modkit on nanopore sequencing data.
by
andres.firrincieli
3.9k
On the DMR tutorial page, they retrieve Human CpG Islands from the UCSC Table Browser. You can do the same thing for Mouse CpG Islands. Hav…
Comment: After doublet detection in 10x scRNA-Seq data, there is still evidence of double
by
yura.grabovska
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Yes but the problem is that biological knowledge is never fully encompasing. While I can say that a cell expressing CD45 alongside astrocyt…
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by
yura.grabovska
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Human genome, ideally hg38
Comment: Is Newbler still available for download
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Hello, I am wondering if you got access to the software or not. I am looking to use Newbler for my research and I contacted Roche too but t…
Comment: Errors with RSEM/bowtie2
by
GenoMax
154k
> I have previously run Trinity with the Trimmomatic step as well as CD-HIT-EST. I assume you did the above to generate the transcriptome,…
Answer: With 10x Visum HD : How to map the barcode id to the barcode sequence ?
by
sacha
★ 2.5k
Got it https://kb.10xgenomics.com/hc/en-us/articles/115004506263-What-is-a-barcode-inclusion-list-formerly-barcode-whitelist
Comment: limmaFit error when comparing TCGA RNA-Seq data
by
vernonlim98
• 0
Thanks for the advice. Am still new to RNA seq and R programming in general, and will just do the analysis by myself.
Answer: The Biostar Handbook. A bioinformatics e-book for beginners.
by
Istvan Albert
103k
Update as of **August 2025**: The book now includes a new online course: [**FastTrack Bioinformatics 2025**][course] at no extra cost. Bio…
Comment: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
by
GenoMax
154k
Likely because the SRA programs are doing database look ups as they retrieve the data where as with `curl` you are simply getting pre-exist…
Comment: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
by
Guilherme
• 0
That's a great tool! I wonder why downloading through curl via SRA's ftp is SO MUCH faster than through sra toolkit programs (fastq-dump or…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
GenoMax
154k
> they should align with common sequence at some ends, making one longer consensus sequence. You don't want to simulate reads like this.…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
cmdcolin
★ 4.3k
I believe the pairs will only have common sequence (e.g. the pairs will "overlap") if the insert size is small. the wgsim program has a fla…
Answer: X chromosome male and female imputation
by
barslmn
★ 2.4k
I think it's better to use the original 900 variants. If you have genotype information, you can get the fraction of heterozygous variants t…
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