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187 results • Page
1 of 4
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76
votes
72
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
2 days ago by
Kevin Blighe
87k
8
votes
16
replies
814
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 12 hours ago by
bk11
★ 2.4k • written 7 weeks ago by
Sofia
• 0
5
votes
13
replies
1.2k
views
Tutorial:
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
R
Ubuntu
Linux
Bioconductor
Rstudio
updated 6 days ago by
ATpoint
82k • written 7 days ago by
BioinfGuru
★ 1.7k
0
votes
13
replies
3.5k
views
6 follow
Tax4Fun2 - error
tax4fun
silva
blast
updated 2 days ago by
drstalinantony28
• 0 • written 2.2 years ago by
ymj
▴ 10
0
votes
11
replies
486
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
1 day ago by
atowns21
• 0
19
votes
11
replies
14k
views
10 follow
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated 13 hours ago by
cmdcolin
★ 3.8k • written 10.8 years ago by
J.F.Jiang
▴ 920
4
votes
11
replies
529
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 3 days ago by
LauferVA
4.2k • written 4 days ago by
dominickd
• 0
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 3 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
1
vote
10
replies
918
views
Troubleshooting RNA-seq data with DNA contamination
RNA-seq
DESeq2
DNA-contamination
2 days ago by
DKA
▴ 40
3
votes
9
replies
433
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 2 days ago by
Istvan Albert
100k • written 4 days ago by
Κοσμάς
• 0
2
votes
9
replies
826
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 3 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
0
votes
8
replies
321
views
Error in cnetplot enrichplot package
R
updated 4 days ago by
Ram
43k • written 4 days ago by
Farhad
• 0
4
votes
8
replies
387
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 30 minutes ago by
Alex Reynolds
35k • written 23 hours ago by
J
▴ 10
1
vote
8
replies
777
views
Adding CB tag to bam file
samtools
bam
updated 1 day ago by
Pierre Lindenbaum
161k • written 8 days ago by
Maria
• 0
8
votes
8
replies
546
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 1 day ago by
swbarnes2
14k • written 2 days ago by
Aaliya
▴ 10
3
votes
8
replies
1.3k
views
Cellranger count error: Input FASTQ file ended prematurely
scRNA-seq
Cellranger
STARsolo
10X
updated 6 days ago by
swbarnes2
14k • written 9 months ago by
bp22
▴ 80
7
votes
7
replies
5.9k
views
6 follow
How to know synonymous and non-synonymous SNPs from transcriptome sequencing
RNA-Seq
SNP
updated 1 day ago by
rohitsatyam102
▴ 850 • written 6.1 years ago by
smallfish
▴ 10
4
votes
7
replies
421
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 1 day ago by
dsull
★ 5.8k • written 2 days ago by
VITALA
• 0
0
votes
7
replies
304
views
Low mapping rate with Salmon
RNA-seq
Salmon
Quantification
updated 12 hours ago by
GenoMax
141k • written 4 days ago by
Patadu94
• 0
10
votes
6
replies
9.8k
views
6 follow
CDS vs cDNA vs transcript for mapping RNA-Seq reads
Assembly
rna-seq
alignment
updated 18 hours ago by
Antonio R. Franco
★ 5.1k • written 6.0 years ago by
williamsbrian5064
▴ 510
0
votes
6
replies
310
views
How to slice a gvcf file with tabix?
Tabix
bed
gvcf
3 days ago by
Sd
• 0
2
votes
6
replies
3.6k
views
Treatment VS Control in Single Cell RNAseq analysis
Single Cell
RNAseq
DE Analysis
Treatment
Control
updated 1 day ago by
ATpoint
82k • written 3.9 years ago by
grayapply2009
▴ 280
3
votes
6
replies
650
views
Ciriquant not configuring hisat2 indexed files
ciriquant
5 days ago by
Atul K.
• 0
3
votes
6
replies
436
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 7 hours ago by
GenoMax
141k • written 3 days ago by
qudrat.nii
▴ 10
7
votes
6
replies
474
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
5 days ago by
analyst
▴ 30
0
votes
6
replies
431
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 2 days ago by
gv
• 0 • written 25 days ago by
Srinka
▴ 20
2
votes
6
replies
1.8k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 5 hours ago by
Ram
43k • written 2.3 years ago by
SYOSY
▴ 10
2
votes
5
replies
452
views
Marking duplicates using UMIs
Deduplication
UMI
updated 4 days ago by
i.sudbery
19k • written 6 days ago by
Lipika
• 0
1
vote
5
replies
329
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
3 days ago by
anasjamshed
▴ 120
2
votes
5
replies
10k
views
Find Corresponding Author: Doi Metadata?
updated 5 days ago by
Ram
43k • written 10.6 years ago by
Louis
▴ 150
0
votes
5
replies
300
views
Should I remove unlocalized scaffols in reference genome before alignment?
qualimap
alignment
minimap2
GRCh38
assembly
2 days ago by
njornet
▴ 20
3
votes
5
replies
548
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
4 days ago by
n_navy
• 0
2
votes
5
replies
282
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
20 hours ago by
Lada
▴ 30
1
vote
5
replies
273
views
Downsampling fastq file
downsample
fastq
updated 4 days ago by
Ram
43k • written 4 days ago by
marco.barr
▴ 80
8
votes
5
replies
5.2k
views
ISCN annotation for SV/CN VCF files
SV
written 7 months ago by
a.beggs
▴ 60
1
vote
5
replies
265
views
ScRNA data question
scRNA
Vlnplot
Samples
updated 13 hours ago by
bk11
★ 2.4k • written 1 day ago by
starswillfade
▴ 10
2
votes
5
replies
522
views
MEGA Analysis Issue on High-Performance Computer
phylogenetic-analysis
MEGA
updated 6 days ago by
Mensur Dlakic
★ 27k • written 7 days ago by
Giorgio
• 0
0
votes
5
replies
297
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 1 day ago by
GenoMax
141k • written 2 days ago by
Bertalan_Takacs
▴ 90
0
votes
5
replies
5.9k
views
Calculate Radius of Gyration [python 3]
biopython
PDB
Radius of Gyration
python
protein
updated 6 days ago by
ndm1096
• 0 • written 6.5 years ago by
ac.research
▴ 30
3
votes
5
replies
267
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 4 days ago by
GenoMax
141k • written 4 days ago by
ahmad.sajad4541
• 0
2
votes
5
replies
687
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 6 days ago by
Jesse
▴ 740 • written 7 days ago by
Ann
★ 2.4k
1
vote
5
replies
591
views
Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Series Library Preparation Set?
cell-barcode
MGI
DNBelab
single-cell
updated 3 days ago by
atowns21
• 0 • written 3 months ago by
benjamin.pyenson
• 0
2
votes
4
replies
307
views
What is the real meaning of relative enrichment/peak height of ChIP-seq tracks?
ChIP-seq
3 days ago by
HyperEvo
• 0
1
vote
4
replies
423
views
GTF file for Rhinolophus sinicus
Rhinolophus-sinicus
GTF
updated 3 days ago by
atowns21
• 0 • written 5 months ago by
ara
• 0
0
votes
4
replies
2.7k
views
Lositan freezing when generating selection table
LOSITAN
updated 2 days ago by
evawillms183
• 0 • written 8.3 years ago by
andrepleao
• 0
3
votes
4
replies
659
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
4 days ago by
rahu
• 0
3
votes
4
replies
251
views
Why gatk VariantAnnotator required bam and coverage files
gatk
VariantAnnotator
1 day ago by
QX
• 0
0
votes
4
replies
254
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
1 day ago by
sehriban.buyukkilic
▴ 10
2
votes
4
replies
297
views
Should I use unpaired reads from trimmomatic
RNA-seq
QC
Trimmomatic
updated 3 days ago by
swbarnes2
14k • written 4 days ago by
dxj294
• 0
2
votes
4
replies
271
views
interpretartion of a vcf file
calling
genotype
ONT
variant
diploid
2 days ago by
samuelkalandarov2002
▴ 10
187 results • Page
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Answer: A faidx-indexed FASTA format file or a FASTA format file
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Answer: Limma Analysis Agilent Microarray Data (GPL1708)
by
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There is an Agilent array case study in the limma User's Guide, which you might find helpful and somewhat simpler. I don't recommend col…
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The comparison you've done seems exactly right to test whether treatment 2 modifies or enhances the effect of treatment 1. Have you exam…
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There's no need for form a contrast because the score variable is already in the model. A continuous variable is its own contrast. Just run…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Oh wow, bedops closest-features, how did I overlook that!! I didn't know about it; thank you! Your awk script is beautiful. This worked per…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Thank you Pierre! I'll try this out with my full data set.
Answer: DNA methylation preprocessing
by
cao510927
▴ 30
I happend to see one paper doing the similar thing, share it with you guys, "A systematic evaluation of normalization methods and probe rep…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
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35k
I think Pierre's answer might be correct for your test input, but it doesn't account for the start position of the reference element and so…
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You need to be clear about how `Seurat` defines highly variable genes here. Highly variable genes are the genes that have very high express…
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by
GenoMax
141k
Please include a link when you are recommending a tool. There can be programs with similar names.
Answer: Heatmap and rna-seq
by
dsull
★ 5.8k
Check out clustergrammer from Avi Ma'ayan's lab.
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
Ram
43k
Simple: ```r data <- lapply(files, read_tsv) data <- Reduce(merge, data) # or Reduce(full_join, data) ``` <a href="" title="Text added bec…
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Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon
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i.sudbery
19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
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If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
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