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Answer: RNA-Seq Data Analysis Workshop (March 11 - 14, 2024 in Berlin, Germany)
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We already have an incredible number of registrations for this time. If you still want to be there, then you should register soon!
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Can you please ask a precise question rather than posting a wall of code? What is the main problem you have, what did you try? Did you foll…
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sevda
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Hello,
Thank you for your attention.
It ran successfully and I got no errors or warnings but no new files were created.
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The system that we used was [Aviti][1]. Our sequencing core was the one who quantified the loading/library concentration. Sorry I don't hav…
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I modified the answer with an approach for that use case.
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OP is looking for overlapping genes - genes with presumably different gene IDs that share some loci. I think the `genes.bed` creation logic…
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Note: The file `snps.bed` will be very large. You'll need sufficient disk space for this step.
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Given files `genes.bed` and `snps.bed`, you could do something like:
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You can use [AGAT][1] to process your annotation file to add the missing exons. I believe `agat_convert_sp_gxf2gxf.pl -g no_exons.gff -o wi…
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and you got answer that should be validate (green tick), or people asked you questions but got no answers.
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Could you tell us which sequencer you used, how you quantified your library size and concentration, and what your loading concentration was…
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I should have mentioned, we ran both Read 1 and 2 well past the points of interest. For each read we ran 75 cycles.
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