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121,953 results • Page
160 of 2440
Sort: Rank
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Votes
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1
vote
0
replies
1.2k
views
Tutorial:
How to convert sra files to fastq files using parallelized fastq-dump
fastq
sra
20 months ago by
Ming Tommy Tang
★ 4.7k
1
vote
3
replies
2.2k
views
Is it necessary to integrate again samples when subclustering
scRNA-seq
integration
Single-cell
updated 6 months ago by
S
• 0 • written 20 months ago by
mropri
▴ 170
7
votes
5
replies
6.0k
views
How to get consensus FASTA sequence from GFA assembly graph?
gfa
consensus
updated 20 months ago by
Adam Taranto
▴ 40 • written 6.8 years ago by
shenwei356
8.7k
3
votes
5
replies
8.3k
views
Creating assembly graphs .gfa
assembly
gfa
graph
bandage
visualization
updated 20 months ago by
Adam Taranto
▴ 40 • written 5.7 years ago by
ricardoguerreiro2121
▴ 80
1
vote
3
replies
1.2k
views
Re-format sample columns in VCF file
vcf
updated 20 months ago by
Ram
45k • written 20 months ago by
phinguyen
• 0
0
votes
3
replies
875
views
Trying to have just one legend for many pie charts
R
updated 20 months ago by
Trivas
★ 1.9k • written 20 months ago by
peavy
• 0
0
votes
0
replies
531
views
How to use --chr flag (vcftools --LROH) with a vcf file that uses scaffold notation
vcftools
scaffold
ROH
20 months ago by
ekirsch
• 0
1
vote
3
replies
1.1k
views
Extracting taxonomic information for species from NCBI using command line
NCBI
taxid
taxonomy
updated 20 months ago by
GenoMax
154k • written 20 months ago by
Mani
• 0
1
vote
4
replies
2.0k
views
Question on the best blast search strategy against NCBI nucleotide database or downloaded genomes through Datasets portal
Blast
NCBI
20 months ago by
Mani
• 0
1
vote
3
replies
1.4k
views
Formatting issues using DADA2 with the database EukRIbo
DADA2
Silva
fasta
metabarcoding
EukRibo
19 months ago by
Begonia_pavonina
▴ 210
4
votes
9
replies
2.6k
views
--normalizeUsing RPGC
bamcompare
ChIP-Seq
deeptools
20 months ago by
Allan
• 0
0
votes
3
replies
1.1k
views
Running Deseq2 with all samples vs samples for each comparison separately
Deseq2
DGE
updated 20 months ago by
swbarnes2
15k • written 20 months ago by
manuelmourato25
• 0
0
votes
3
replies
1.1k
views
Enrichement with clusterProfiler and biomartr
clusterProfiler
biomartr
Zea-mays
updated 20 months ago by
jv
★ 1.9k • written 20 months ago by
san96
▴ 190
0
votes
2
replies
758
views
Change in the identified Gene3D domain
HMMER
InterPro
Gene3D
Domain
20 months ago by
GenesisBio
• 0
2
votes
3
replies
985
views
VEP - select consequences version
VEP
updated 20 months ago by
GenoMax
154k • written 20 months ago by
gernophil
▴ 130
6
votes
23
replies
4.7k
views
How to speed Salmon index up
metagenome
genome
salmon
20 months ago by
young_bioinformatician
▴ 240
2
votes
3
replies
1.7k
views
Alignment of RNASeq with STAR for variant calling
STAR
variant-calling
RNA-seq
alignment
updated 20 months ago by
Ram
45k • written 20 months ago by
Gilles
▴ 10
5
votes
5
replies
1.7k
views
VEP versions
genomics
variants
vep
updated 20 months ago by
GenoMax
154k • written 20 months ago by
Sammy
▴ 30
2
votes
1
reply
887
views
EnhancedVolcano not plotting any dots
volcano-plot
enhancedvolcano
ggplot2
updated 20 months ago by
Ram
45k • written 20 months ago by
Bonita
▴ 30
4
votes
3
replies
1.3k
views
Double counts with bedtools intersect?
bedtools
mapping
alignment
19 months ago by
Zeng Hao
▴ 40
1
vote
1
reply
698
views
Gene expression of microarray data
Microarray
updated 20 months ago by
Gordon Smyth
★ 8.3k • written 20 months ago by
CTLong
▴ 140
2
votes
4
replies
1.2k
views
Bacterial genome analysis - how the check if newly sequenced genome has errors
genome
next-gen
alignment
20 months ago by
Veselina
• 0
2
votes
2
replies
1.1k
views
Microarray analysis using Limma
microarray
voom
limma
20 months ago by
mrashad
▴ 80
0
votes
1
reply
589
views
MGnify_metagenomics-Biopython
Biopython
updated 20 months ago by
Mensur Dlakic
★ 30k • written 20 months ago by
Kominist Asmamaw
• 0
0
votes
2
replies
829
views
Cross-referencing multiple databses with PDB
genbank
PDB
uniprot
embl-ebi
refseq
updated 20 months ago by
Mensur Dlakic
★ 30k • written 20 months ago by
Mariana
▴ 50
0
votes
10
replies
3.3k
views
ONT Raw Read Sequencing Pipeline
remora
dorado
ONT
Basecall
20 months ago by
turcoa1
• 0
2
votes
2
replies
3.6k
views
Is MACS3 the same as MACS2?
MACS
MACS3
MACS2
updated 20 months ago by
GenoMax
154k • written 20 months ago by
cwwong13
▴ 40
1
vote
5
replies
2.3k
views
Tutorial:
Single-cell RNA-seq: Annotation: Celltype annotation migration(mapping) with TOSICA
scRNA-seq
TOSICA
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
1
vote
3
replies
1.3k
views
Problems going from biopython motif to logo
logo
biopython
motif
20 months ago by
Emilio
• 0
1
vote
9
replies
1.7k
views
Problem with ONT sequencing pipeline. Probably in the Samtools part
bam
samtools
ONT
updated 20 months ago by
GenoMax
154k • written 20 months ago by
Emilio
• 0
5
votes
3
replies
2.1k
views
salmon with long reads
salmon
updated 20 months ago by
Rob
7.1k • written 20 months ago by
shinyjj
▴ 60
3
votes
3
replies
1.1k
views
Is there an archive for Ensembl Metazoa releases?
ensembl
metazoa
20 months ago by
Michael
56k
0
votes
3
replies
826
views
Low number of reads - demultiplex issue? scRNAseq on NextSeq500
scRNA-seq
illumina
nextseq500
updated 20 months ago by
Ram
45k • written 20 months ago by
Berina
• 0
0
votes
9
replies
1.7k
views
output same result using different imputed data and same reference panel
PHG
updated 20 months ago by
pjb39
▴ 220 • written 21 months ago by
sh
• 0
0
votes
1
reply
1.4k
views
News:
LAST CALL: Online Course - A Practical Introduction to NGS Data Analysis (February 28 - March 1, 2024)
NGS
Online-Workshop
updated 20 months ago by
Ram
45k • written 20 months ago by
David Langenberger
11k
13
votes
7
replies
6.7k
views
Forum:
How to learn bioinformatics?
learning
updated 20 months ago by
Samuel Lampa
★ 1.3k • written 3.4 years ago by
sakichwan
▴ 20
2
votes
5
replies
1.5k
views
batch effect correction in 10x genomics single cell sequencing
scRNA-seq
updated 20 months ago by
QX
▴ 80 • written 20 months ago by
Sara
▴ 280
0
votes
7
replies
2.0k
views
UMI-tools no tsv files at deduplication
deduplication
NGS
UMI
DNA-seq
UMI-tools
updated 20 months ago by
Ram
45k • written 20 months ago by
Sarah
• 0
2
votes
4
replies
1.1k
views
tools for closing gaps and construct a complete chromosome in Galaxy
Galaxy
SPADEs
updated 20 months ago by
Ram
45k • written 20 months ago by
Bioinformática
▴ 10
1
vote
3
replies
1.8k
views
Bacterial Synteny Tools
comparative
bacteria
synteny
updated 8 months ago by
Will
• 0 • written 20 months ago by
John
• 0
2
votes
2
replies
1.1k
views
number of homozygotes with phenotype
plink
gwas
20 months ago by
ccagg
▴ 60
0
votes
4
replies
1.9k
views
cell ranger low correct barcodes and failed
scRNA-seq
cellranger
sratoolkit
single-cell
updated 20 months ago by
Ram
45k • written 20 months ago by
shersky
• 0
0
votes
1
reply
908
views
homer findMotifsGenome.pl
chip-seq
atac-seq
homer
updated 20 months ago by
Trivas
★ 1.9k • written 20 months ago by
biology_inform
▴ 60
1
vote
7
replies
1.6k
views
both log2 and log2fc giving a completely huge difference of a gene in subpopulations
loupe-browser
single-cell-sequencing
20 months ago by
am-cell
• 0
3
votes
2
replies
2.2k
views
Tutorial:
Single-cell RNA-seq: Preprocessing: Data integration and batch correction-2
scRNA-seq
updated 20 months ago by
ATpoint
89k • written 20 months ago by
Julia Ma
▴ 140
2
votes
8
replies
1.8k
views
Problem in Salmon when indexing Rattus_norvegicus
salmon
updated 20 months ago by
dsull
★ 7.7k • written 20 months ago by
dzisis1986
▴ 70
1
vote
1
reply
642
views
How to quantify gaps in multiple sequence alignments
blast
alignment
updated 20 months ago by
Dunois
★ 2.9k • written 20 months ago by
dec986
▴ 380
26
votes
27
replies
6.1k
views
10 follow
Forum:
Determining computer time required
computer
hpc
time
updated 20 months ago by
dsull
★ 7.7k • written 20 months ago by
Rozita
▴ 40
0
votes
1
reply
506
views
Parameter bSubControl with no effect in dba.count?
DiffBind
updated 20 months ago by
Ram
45k • written 20 months ago by
frueher
• 0
0
votes
0
replies
485
views
KEGG PATHWAY ANALYSIS
Transcriptome
20 months ago by
nesanemashudu
• 0
121,953 results • Page
160 of 2440
Recent Votes
Modernized RNA-MuTect pipeline for tumor-only RNA-seq somatic variant calling
Dictys refuses to use CUDA supported pytorch during installation
Answer: Dictys refuses to use CUDA supported pytorch during installation
Comment: Looking to learn practical bioinformatics
Answer: MAPQ algorithms of Giraffe
Answer: MAPQ algorithms of Giraffe
Answer: Illumina Instrument Type from fastq?
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Recent Awards •
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Popular Question
to
Ibrahim Tanyalcin
★ 1.2k
Teacher
to
colindaven
8.0k
Popular Question
to
m90
▴ 30
Popular Question
to
PolenP
• 0
Popular Question
to
xiaoleiusc
▴ 140
Teacher
to
cfos4698
★ 1.2k
Popular Question
to
sacha
★ 2.5k
Recent Replies
Answer: X chromosome male and female imputation
by
barslmn
★ 2.4k
I think it's better to use the original 900 variants. If you have genotype information, you can get the fraction of heterozygous variants t…
Comment: Slam-Seq Conversion Rate per Read
by
michael.ante
★ 4.0k
Try to switch to percentage view of this graph. Stacked bar charts with different heights can sometimes be misleading.
Comment: Functional prediction for proteins of bacteria.
by
m90
▴ 30
Biochemical changes
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
PolenP
• 0
Sorry about that. It's when I align the paired reads together like the left and righ, they should align with common sequence at some ends, …
Answer: Dictys refuses to use CUDA supported pytorch during installation
by
zwjiang
▴ 30
I fixed my problem by ``` conda create -y -p /project/spott/zwjiang/conda_envs/dictys_gpu -c conda-forge python=3.9 mamba conda activat…
Comment: HIV : gtf or gff annotation
by
Ales
▴ 50
Thank you for your response! We have just posted the full preprint to BioRxiv (https://www.biorxiv.org/content/10.1101/2025.09.24.675449v1)…
Comment: Does this adapter content from fastqc to warrant trimming prior to alignment?
by
curious
▴ 890
Just adding some detail here that may help draw some connections with other posts, I did a bunch of sleuthing with selectively trimming dif…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
cmdcolin
★ 4.3k
It's a little unclear what you mean by "I was able to use wgsim, but when I tried aligning the paired-reads, it's not aligning together."
Comment: using packages with conda
by
ATpoint
89k
Just to be sure: You ssh into a remote server, correct? Die you setup the conda environment on your local machine or the remote? Must be th…
Answer: pySCENIC aucell matrix is all 0s
by
Tripfantasy
• 0
Sorry to those of you having the same issue. I haven't been on here in quite some time! I've documented my fix on pySCENIC's github page be…
Comment: [vg giraffe] Unable to add read group to BAM file
by
anovak
▴ 180
I think this is still not quite right because I think your tags need to be tab-separated in the output to really be parseable, and here the…
Answer: MAPQ algorithms of Giraffe
by
anovak
▴ 180
`vg giraffe` does not use the exact same MAPQ algorithm as bwa-mem. However, its mapping qualities are still meant to be Phred-scale probab…
Comment: using packages with conda
by
raquel
• 0
Yes, I'm using SSH session
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
Mensur Dlakic
★ 30k
I think only a fraction of sequencing errors can be specified. If a mix of a reference and a mutated genome is included, setting sequencing…
Comment: using packages with conda
by
ATpoint
89k
Cannot reproduce. Is this run via some sort of server/HPC/scheduler/something?
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