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121,954 results • Page
161 of 2440
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0
votes
0
replies
486
views
KEGG PATHWAY ANALYSIS
Transcriptome
20 months ago by
nesanemashudu
• 0
4
votes
5
replies
1.8k
views
Featurecounts has more reads than star assigned reads ?
featurecounts
STAR
RNA-seq
20 months ago by
PK
▴ 130
2
votes
3
replies
1.3k
views
Job:
Bio-informatics Scientist at BASF | Nunhems (Near Eindhoven, The Netherlands)
BASF
20 months ago by
WS_jobs
• 0
0
votes
2
replies
890
views
Specific error in R
gene-annotation
updated 20 months ago by
Ram
45k • written 20 months ago by
e.fajeiii
• 0
5
votes
8
replies
1.7k
views
Multiple threads on ubuntu server
Ubuntu
updated 20 months ago by
colindaven
8.0k • written 20 months ago by
oghzzang
▴ 50
0
votes
1
reply
616
views
Understanding SNP Coordinates for .bed File Creation
VCF
bed
updated 20 months ago by
Ram
45k • written 20 months ago by
iarmir
▴ 10
2
votes
1
reply
834
views
Combination of genomic datasets genotyped using different arrays
genotyping
GWAS
imputation
inflation
updated 20 months ago by
Juliana
▴ 50 • written 2.7 years ago by
desicasares
▴ 40
2
votes
2
replies
2.0k
views
How to create a metagene profile plot
EU-seq
metagene-profile
RNA-seq
20 months ago by
rebeccadistefano8
• 0
0
votes
3
replies
1.4k
views
WGCNA input genes - greatest variance?
WGCNA
RNA-Seq
updated 20 months ago by
andres.firrincieli
3.9k • written 20 months ago by
fluentin44
▴ 20
0
votes
1
reply
553
views
SSR marker data
marker
SSR
updated 20 months ago by
Ram
45k • written 20 months ago by
m.esmaeilpour
▴ 10
0
votes
2
replies
1.5k
views
Representation of SV(genomic structural variation) with Circos plots
Circos
SVs
20 months ago by
Domi
• 0
1
vote
2
replies
1.1k
views
filtering lowly expressed reads
RNA-Seq
Differential-Expression
limma
updated 20 months ago by
Gordon Smyth
★ 8.3k • written 20 months ago by
Shaimaa Gamal
▴ 10
0
votes
0
replies
440
views
Classify Phage Taxonomy Scaffold Data (Genome Fractions)
Phages
PhaBOX
Bacteria
Genome
PhaGCN
20 months ago by
Bioinformatics_begginner
▴ 20
6
votes
7
replies
1.5k
views
NF-Core ampliseq - Rscript markdown error
nf-core
ampliseq
updated 20 months ago by
Michael
56k • written 20 months ago by
nermze
• 0
0
votes
3
replies
670
views
Reference transcriptome
Reference-transcriptome
updated 20 months ago by
Ram
45k • written 20 months ago by
ag17007
• 0
1
vote
1
reply
769
views
how arrange samples columns according to row names from another file in R
statistics
r
biostatistics
updated 20 months ago by
rpolicastro
13k • written 20 months ago by
Bioinfonext
▴ 480
0
votes
2
replies
2.1k
views
cd-hit-est total seq value doesn't match number of sequences being compared?
metagenomics
cd-hit-est
CD-HIT
binning
filtering
updated 20 months ago by
Sriram
• 0 • written 6.9 years ago by
c.e.chong
▴ 60
3
votes
3
replies
868
views
Blastx -max_target_seqs
max_target_seqs
Blastx
updated 20 months ago by
GenoMax
154k • written 20 months ago by
sansan
• 0
0
votes
0
replies
518
views
Question on Unexpected Strandness Results in RNA-seq Analysis Using Leafcutter and Regtools
leafcutter
regtools
strandness
20 months ago by
manota
• 0
0
votes
0
replies
559
views
What is the appropricate model in GAPIT?
GAPIT
linear-model
phenotype
updated 20 months ago by
Ram
45k • written 20 months ago by
dragon
• 0
1
vote
4
replies
1.8k
views
how to convert using sratoolkit or fast-dump
single
fasterq-dump
sratoolkit
cell
fastq-dump
updated 20 months ago by
GenoMax
154k • written 20 months ago by
shersky
• 0
1
vote
1
reply
890
views
eQTL analysis using GWAS summary statistics only
R
SNP
eQTL
updated 20 months ago by
LChart
5.1k • written 20 months ago by
Sebastian
▴ 10
0
votes
0
replies
1.1k
views
cellchat analysis from from a Scanpy h5ad file
scanpy
r
h5ad
CellChat
cellchat
20 months ago by
cgp09741
▴ 10
4
votes
9
replies
1.9k
views
Correlate number of cells with gene expression of one gene in scRNA Seq
Seurat
updated 20 months ago by
Zhenyu Zhang
★ 1.3k • written 21 months ago by
Nona
▴ 90
1
vote
4
replies
1.4k
views
How to replace the sequence in the genome.fasta with reverse complement in specific chromosome and location.
genome
awk
edditing
bash
python
updated 20 months ago by
GenoMax
154k • written 20 months ago by
Ap1438
▴ 50
0
votes
1
reply
886
views
How can I calculate the percent of bases covered in the query assembly relative to the reference assembly?
samtools
updated 20 months ago by
dthorbur
★ 3.1k • written 20 months ago by
O.rka
▴ 750
1
vote
2
replies
793
views
Qualimap assigns more reads to exonic regions than total reads in the sample
rnaseq
qualimap
20 months ago by
Cris Tuñí
• 0
3
votes
2
replies
1.0k
views
how to merge two files without duplicating same column
R
statistics
biostatistics
updated 20 months ago by
DBScan
▴ 530 • written 20 months ago by
Bioinfonext
▴ 480
1
vote
1
reply
1.0k
views
DNA sequencing workflow involving UMIs
dna
umi
sequencing
updated 20 months ago by
Matthias Zepper
5.1k • written 20 months ago by
ww22runner
▴ 60
0
votes
1
reply
1.8k
views
MinKnow installation on Ubuntu 22.01
Minknow
Ubuntu
updated 20 months ago by
Ram
45k • written 20 months ago by
peer.schmitz
• 0
2
votes
1
reply
2.4k
views
Tutorial:
Single-cell RNA-seq: Preprocessing: Clustering space and evaluation
scRNA-seq
space
clustering
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
2
votes
3
replies
1.6k
views
DiffBind dba.normalize error using spikein reads
DiffBind
ChIP-seq
updated 20 months ago by
Yaqin
• 0 • written 3.6 years ago by
adrian.rodriguez
▴ 10
0
votes
1
reply
1.3k
views
Tutorial:
Single-cell RNA-seq: Annotation: Celltype auto annotation with MetaTiME
scRNA-seq
MetaTiME
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
1
vote
0
replies
813
views
Herald:
The Biostar Herald for Monday, January 15, 2024
herald
20 months ago by
Biostar
3.6k
0
votes
0
replies
925
views
Tutorial:
Single-cell RNA-seq: Annotation: Celltype auto annotation with SCSA-3
scRNA-seq
SCSA
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
0
votes
0
replies
880
views
Tutorial:
Single-cell RNA-seq: Annotation: Celltype auto annotation with SCSA-2
scRNA-seq
SCSA
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
1
vote
0
replies
1.0k
views
Tutorial:
Single-cell RNA-seq: Annotation: Celltype auto annotation with SCSA
scRNA-seq
SCSA
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
0
votes
0
replies
1.1k
views
Tutorial:
Single-cell RNA-seq: Preprocessing: Data integration and batch correction-3
scRNA-seq
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
1
vote
0
replies
1.2k
views
Tutorial:
Single-cell RNA-seq: Preprocessing: Data integration and batch correction
scRNA-seq
updated 20 months ago by
Ram
45k • written 20 months ago by
Julia Ma
▴ 140
0
votes
0
replies
420
views
cross-correlation peak at position 0 when calculating ChIp-Seq
cross-correlation
peak
chip-seq
phantompeak
ChIPseeker
20 months ago by
Assa Yeroslaviz
★ 1.9k
7
votes
7
replies
9.0k
views
6 follow
Variant calling on Nanopore reads
genome
alignment
updated 20 months ago by
colindaven
8.0k • written 5.7 years ago by
felix
▴ 20
0
votes
1
reply
752
views
How to annotate phage genomes?
genome
phage
annotation
updated 20 months ago by
Ram
45k • written 20 months ago by
marongiu.luigi
▴ 760
7
votes
2
replies
2.2k
views
News:
NGS Data Analysis Workshops 2024
scRNA-seq
dna-seq
rna-seq
nextflow
20 months ago by
David Langenberger
11k
0
votes
1
reply
661
views
RNA Type Detection
rna-seq
rna
types
20 months ago by
kyildizn
• 0
0
votes
5
replies
1.7k
views
Stampy make fails
Stampy
updated 20 months ago by
randolphquek
• 0 • written 4.2 years ago by
shpak.max
▴ 60
0
votes
0
replies
388
views
How can I do GWAS with SNP array result in plink?
plink
SNP
GWAS
array
20 months ago by
y1310
• 0
0
votes
0
replies
796
views
Tutorial:
How to Label the Y Axis with percentage sign and Order Bars in ggplot2 using a single cell dataset
seurat
single-cell
ggplot2
20 months ago by
Ming Tommy Tang
★ 4.7k
2
votes
4
replies
1.6k
views
How to get only polymorphic genotypes from combined vcf file filtering out homozygous, heterozygous and missing genotypes
polymorphic
combinedVCF
genotypes
updated 20 months ago by
dthorbur
★ 3.1k • written 20 months ago by
analyst
▴ 70
22
votes
7
replies
21k
views
6 follow
GFA to Fasta file
sequence
updated 20 months ago by
Adam Taranto
▴ 40 • written 9.8 years ago by
marcela.uliano
▴ 90
4
votes
2
replies
1.0k
views
How can I know if a gene mutation is somatic or germline?
somatic
mutation
germline
updated 20 months ago by
LauferVA
4.8k • written 20 months ago by
Fares
• 0
121,954 results • Page
161 of 2440
Recent Votes
Modernized RNA-MuTect pipeline for tumor-only RNA-seq somatic variant calling
Dictys refuses to use CUDA supported pytorch during installation
Answer: Dictys refuses to use CUDA supported pytorch during installation
Comment: Looking to learn practical bioinformatics
Answer: MAPQ algorithms of Giraffe
Answer: MAPQ algorithms of Giraffe
Answer: Illumina Instrument Type from fastq?
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Recent Awards •
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Popular Question
to
Ibrahim Tanyalcin
★ 1.2k
Teacher
to
colindaven
8.0k
Popular Question
to
m90
▴ 30
Popular Question
to
PolenP
• 0
Popular Question
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xiaoleiusc
▴ 140
Teacher
to
cfos4698
★ 1.2k
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sacha
★ 2.5k
Recent Replies
Answer: X chromosome male and female imputation
by
barslmn
★ 2.4k
I think it's better to use the original 900 variants. If you have genotype information, you can get the fraction of heterozygous variants t…
Comment: Slam-Seq Conversion Rate per Read
by
michael.ante
★ 4.0k
Try to switch to percentage view of this graph. Stacked bar charts with different heights can sometimes be misleading.
Comment: Functional prediction for proteins of bacteria.
by
m90
▴ 30
Biochemical changes
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
PolenP
• 0
Sorry about that. It's when I align the paired reads together like the left and righ, they should align with common sequence at some ends, …
Answer: Dictys refuses to use CUDA supported pytorch during installation
by
zwjiang
▴ 30
I fixed my problem by ``` conda create -y -p /project/spott/zwjiang/conda_envs/dictys_gpu -c conda-forge python=3.9 mamba conda activat…
Comment: HIV : gtf or gff annotation
by
Ales
▴ 50
Thank you for your response! We have just posted the full preprint to BioRxiv (https://www.biorxiv.org/content/10.1101/2025.09.24.675449v1)…
Comment: Does this adapter content from fastqc to warrant trimming prior to alignment?
by
curious
▴ 890
Just adding some detail here that may help draw some connections with other posts, I did a bunch of sleuthing with selectively trimming dif…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
cmdcolin
★ 4.3k
It's a little unclear what you mean by "I was able to use wgsim, but when I tried aligning the paired-reads, it's not aligning together."
Comment: using packages with conda
by
ATpoint
89k
Just to be sure: You ssh into a remote server, correct? Die you setup the conda environment on your local machine or the remote? Must be th…
Answer: pySCENIC aucell matrix is all 0s
by
Tripfantasy
• 0
Sorry to those of you having the same issue. I haven't been on here in quite some time! I've documented my fix on pySCENIC's github page be…
Comment: [vg giraffe] Unable to add read group to BAM file
by
anovak
▴ 180
I think this is still not quite right because I think your tags need to be tab-separated in the output to really be parseable, and here the…
Answer: MAPQ algorithms of Giraffe
by
anovak
▴ 180
`vg giraffe` does not use the exact same MAPQ algorithm as bwa-mem. However, its mapping qualities are still meant to be Phred-scale probab…
Comment: using packages with conda
by
raquel
• 0
Yes, I'm using SSH session
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
Mensur Dlakic
★ 30k
I think only a fraction of sequencing errors can be specified. If a mix of a reference and a mutated genome is included, setting sequencing…
Comment: using packages with conda
by
ATpoint
89k
Cannot reproduce. Is this run via some sort of server/HPC/scheduler/something?
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