2
votes
3
replies
759
views
3
votes
3
replies
1.4k
views
updated 2.5 years ago by
Gordon Smyth
★
8.3k
•
written 2.5 years ago by
alwayshope
▴
40
5
votes
3
replies
1.7k
views
2.5 years ago by
Confused_human
▴
30
1
vote
3
replies
998
views
2.5 years ago by
tang
•
0
2
votes
2
replies
1.2k
views
6
votes
7
replies
1.8k
views
2.5 years ago by
venkateshbiotech
•
0
1
vote
4
replies
10k
views
updated 2.5 years ago by
Abdullah K
▴
10
•
written 3.9 years ago by
adhamzul
▴
20
0
votes
0
replies
397
views
updated 2.5 years ago by
GenoMax
154k
•
written 2.5 years ago by
shivanit1235
•
0
2
votes
2
replies
1.4k
views
updated 2.5 years ago by
Eijy Nagai
▴
90
•
written 2.5 years ago by
wes
▴
90
1
vote
1
reply
815
views
4
votes
3
replies
1.4k
views
2
votes
2
replies
1.3k
views
2
votes
1
reply
673
views
0
votes
0
replies
599
views
2
votes
3
replies
1.3k
views
0
votes
1
reply
709
views
updated 2.5 years ago by
Jeremy Leipzig
23k
•
written 2.5 years ago by
Emily
•
0
0
votes
2
replies
1.9k
views
1
vote
5
replies
1.5k
views
3
votes
4
replies
1.3k
views
2.4 years ago by
plain_text
•
0
0
votes
3
replies
1.1k
views
updated 2.5 years ago by
Billy Rowell
▴
520
•
written 2.5 years ago by
aveadj
•
0
1
vote
1
reply
803
views
updated 2.5 years ago by
Istvan Albert
103k
•
written 2.5 years ago by
Alireza
•
0
0
votes
0
replies
653
views
2.5 years ago by
Sy80
▴
10
1
vote
1
reply
1.0k
views
0
votes
0
replies
1.3k
views
2.5 years ago by
yuzun
•
0
4
votes
3
replies
1.2k
views
1
vote
2
replies
846
views
2.5 years ago by
Lada
▴
40
0
votes
2
replies
887
views
updated 2.5 years ago by
Istvan Albert
103k
•
written 2.5 years ago by
Fatemeh
•
0
0
votes
4
replies
1.3k
views
updated 2.5 years ago by
Pierre Lindenbaum
166k
•
written 2.5 years ago by
berndmann
▴
10
0
votes
0
replies
538
views
updated 2.5 years ago by
Ram
45k
•
written 2.5 years ago by
malearimond
•
0
0
votes
4
replies
2.0k
views
3
votes
3
replies
1.8k
views
0
votes
0
replies
881
views
0
votes
0
replies
1.6k
views
5
votes
0
replies
1.1k
views
4
votes
6
replies
1.5k
views
5
votes
5
replies
1.3k
views
updated 2.5 years ago by
GenoMax
154k
•
written 2.5 years ago by
sakisugihara1403403
•
0
0
votes
0
replies
529
views
2
votes
3
replies
1.5k
views
updated 2.5 years ago by
Ram
45k
•
written 2.5 years ago by
colindaven
8.0k
7
votes
5
replies
1.9k
views
2.5 years ago by
rj.rezwan
▴
20
0
votes
1
reply
693
views
0
votes
2
replies
945
views
2.5 years ago by
Sau
•
0
0
votes
0
replies
518
views
2.5 years ago by
marina.wakid
▴
10
0
votes
0
replies
1.5k
views
updated 2.5 years ago by
Ram
45k
•
written 2.5 years ago by
Sergio Martínez Cuesta
▴
230
0
votes
1
reply
815
views
3
votes
7
replies
2.1k
views
15
votes
5
replies
4.0k
views
2.5 years ago by
Storm
▴
10
0
votes
1
reply
840
views
4
votes
5
replies
1.7k
views
0
votes
0
replies
533
views
2.5 years ago by
Binghong
▴
20
0
votes
0
replies
418
views
Recent Votes
Recent Locations •
All
United States, 3 minutes ago
UCLA, 7 minutes ago
Germany, 31 minutes ago
Cuba, 32 minutes ago
United Kingdom, 33 minutes ago
Bologna, 50 minutes ago
United States, 52 minutes ago
Recent Awards •
All
Teacher to
andres.firrincieli
3.9k
Teacher to
Aleksandra
▴
180
Popular Question to
vernonlim98
•
0
Popular Question to
EagleEye
7.6k
Scholar to
saamhasan55
▴
30
Recent Replies
Comment: DESeq2 on metagenome KO counts
by
ATpoint
89k
This statement makes zero sense in response to what I wrote.
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
I focused on the fact that DESeq2 models overdispersion, but overlooked its implicit assumption: normalisation works when the main source o…
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
Thank you very much. I mistakenly drew a superficial analogy. I equated KO counters with gene counters.
Comment: DESeq2 on metagenome KO counts
by
ATpoint
89k
Both vst or logcpm will favor expression level (magnitude of counts) rather than differences in a hclust/heatmap. For differences you need …
Comment: DESeq2 on metagenome KO counts
by
ATpoint
89k
The DESeq2 developer has advised many times against DESeq2 for metagenomics. Please search for related posts over at support.bioconductor.o…
Answer: DESeq2 on metagenome KO counts
by
andres.firrincieli
3.9k
I wouldn’t use DESeq2 on aggregated raw counts per KO, because doing so means accepting two pretty big assumptions:
1. All genes with t…
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
(I apologise for my previous reply; I wanted to be as detailed as possible)
DESeq2 handles KO counts perfectly well. For a time-series des…
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
180
I apologise for how that sounded; I wanted to be as detailed as possible.
Comment: GUI commercial software for 10x single cell gene expression analysis
by
Istvan Albert
103k
A comment on: "*Wonky analysis is possible even with open-source R libraries.*"
True - and arguably, the risk of a wonky analysis is actua…
Comment: GUI commercial software for 10x single cell gene expression analysis
by
GenoMax
154k
`Trailmaker` likely only supports Parse's evercode data. <br>
`BioTuring` does not appear to have published pricing. <br>
`Rosalind` appear…
Answer: GUI commercial software for 10x single cell gene expression analysis
by
firestar
★
1.7k
Found a few more when I asked around. I have no idea how good or bad these are. If anyone has used these or knows more about these, feel fr…
Comment: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
GenoMax
154k
> I performed a standard proteomics screening workflow
What database did you use to search against? Perhaps you are using an old (not so c…
Answer: FACS quality control based on size and doublet detection in scRNA-seq
by
ATpoint
89k
No, it's unrelated. Doublets in 10x happen if two cells get enclosed into the same GEM droplet. This has nothing to do with how flow cytome…
Answer: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
Elisabeth Gasteiger
★
2.4k
Apparently you are working with human proteins? I would strongly recommend looking only at proteins that are part of the human reference pr…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
colindaven
8.0k
Wow, thats too long. How much RAM does your machine have, it is likely swapping
Traffic: 3157 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6