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116,974 results • Page
1 of 2340
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Votes
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1
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3
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77
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Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
build
genome
updated 13 minutes ago by
GenoMax
142k • written 4 hours ago by
Ali
• 0
0
votes
3
replies
121
views
How to extract cells of different species after mapping with combined genome?
snrnaseq
scrnaseq
updated 16 minutes ago by
GenoMax
142k • written 14 hours ago by
vk
▴ 10
0
votes
3
replies
51
views
Can I perform a correlation test with 3 biological replicates per condition?
deseq2
RNA-Seq
correlation
gene
replicate
updated 6 minutes ago by
ATpoint
82k • written 2 hours ago by
manuelmourato25
• 0
1
vote
1
reply
180
views
finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame (uORF)"
UTR
masspec
uORF
peptide
1 hour ago by
Pierre Lindenbaum
161k
0
votes
1
reply
49
views
Details on salmon index
Salmon
updated 2 hours ago by
ATpoint
82k • written 3 hours ago by
Lorenzo
• 0
1
vote
8
replies
347
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
updated 47 minutes ago by
ATpoint
82k • written 1 day ago by
egascon
• 0
0
votes
0
replies
33
views
API kegg - IndexError: list index out of range
kegg
API
Error
3 hours ago by
mirwa.zidi93
• 0
0
votes
0
replies
32
views
Blog:
The Current State and Future Prospects of Long-Read Sequencing
LRS
4 hours ago by
Novogene
▴ 420
0
votes
0
replies
36
views
qPCR gene expression data analysis
qPCR
geneexpression
anova
5 hours ago by
ebertomeup
• 0
797
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.8k • written 7.4 years ago by
Istvan Albert
100k
0
votes
1
reply
70
views
Sam file Header problem
Sam
Header
problem
file
updated 5 hours ago by
Pierre Lindenbaum
161k • written 6 hours ago by
saifulislam99121
• 0
0
votes
4
replies
162
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
Degrees
python
Degree
offtopic
6 hours ago by
kuttibiotech2009
▴ 30
0
votes
1
reply
500
views
scRNAseq quality control weird double curve in nFeature vs nCount plot
scRNA-seq
QC
single
cell
BDRhapsody
analysis
updated 8 hours ago by
valdirbarth
▴ 20 • written 12 months ago by
Salomé
• 0
0
votes
0
replies
47
views
select set of intervals that cover a genomic region
GRanges
bed
8 hours ago by
ntsopoul
▴ 60
1
vote
0
replies
56
views
Tool:
Collect dispersed executable files and run them by using bingo.
bingo
8 hours ago by
dwpeng
▴ 10
0
votes
0
replies
47
views
BiomartException: Query ERROR for existing dataset in BioMart
biomart
ensembl
wheat
python
pybiomart
10 hours ago by
Luqman
• 0
1
vote
3
replies
131
views
ggrepel function: geom_text_repel()
ggrepel
ggplo2
R
3 hours ago by
sooni
▴ 20
0
votes
2
replies
113
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
5 hours ago by
mavy
▴ 10
1
vote
1
reply
172
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
15 hours ago by
ramiro.barrantes
• 0
1
vote
2
replies
157
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
1 hour ago by
diqixiaoyaoer
▴ 10
2
votes
6
replies
1.9k
views
How can you *consistently* download BioProject IDs from NCBI's BioSample database using Entrez Direct?
XML
ncbi
entrez-direct
updated 15 hours ago by
Ram
43k • written 3.9 years ago by
millere
• 0
0
votes
1
reply
96
views
What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
updated 3 hours ago by
Michael
54k • written 16 hours ago by
mgranada3
▴ 30
0
votes
0
replies
67
views
Add line under stat test
stats
R
16 hours ago by
Ghada
• 0
1
vote
5
replies
756
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
written 6 months ago by
Daniel
▴ 30
0
votes
0
replies
67
views
DOCK6 installation on MacOS M1
installation
troubleshooting
DOCK6
updated 18 hours ago by
GenoMax
142k • written 18 hours ago by
Rodolfo Adrián
• 0
1
vote
3
replies
154
views
Genotyping sites with N in reference genome
GATK
UnifiedGenotyper
updated 18 hours ago by
Zhenyu Zhang
★ 1.2k • written 19 hours ago by
shpak.max
▴ 50
0
votes
1
reply
114
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 19 hours ago by
yura.grabovska
▴ 70 • written 20 hours ago by
ev97
▴ 20
0
votes
7
replies
244
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
meta
how-to
ncbi
forum
updated 19 minutes ago by
GenoMax
142k • written 20 hours ago by
hophuquy0944
• 0
0
votes
4
replies
189
views
Sequencing Depth (Read Depth) Calculations
depth
9 hours ago by
LucisTheFather
• 0
0
votes
1
reply
101
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
20 hours ago by
asalimih
▴ 60
0
votes
0
replies
75
views
Is it okay to assemble my 12 metatranscriptome samples with Trinity separately?
trinity
metatranscriptome
20 hours ago by
jway
• 0
2
votes
2
replies
139
views
Cannot install bwa-mem2 via conda
conda
mamba
python
code
updated 20 hours ago by
Istvan Albert
100k • written 21 hours ago by
jsmith120f
• 0
2
votes
2
replies
164
views
genome finishing
finishing
genome
updated 21 hours ago by
nd48
▴ 20 • written 1 day ago by
trezini
• 0
1
vote
5
replies
180
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 21 hours ago by
Pierre Lindenbaum
161k • written 22 hours ago by
biology_inform
▴ 50
1
vote
4
replies
250
views
Add stats to boxplot in R
stats
R
18 hours ago by
Ghada
• 0
0
votes
1
reply
97
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 1 hour ago by
Papyrus
★ 2.9k • written 22 hours ago by
yura.grabovska
▴ 70
0
votes
0
replies
83
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
22 hours ago by
Lélé
▴ 10
0
votes
4
replies
202
views
calculating genomic coverage/ base overlap in R
bioinoformatics
genomics
2 hours ago by
Sayantani
• 0
1
vote
4
replies
182
views
Annotating file using bcftools
annotation
plink
bcftools
updated 3 hours ago by
Pierre Lindenbaum
161k • written 1 day ago by
kl
▴ 10
0
votes
9
replies
311
views
Duplicated reads (IDs) from nanopore sequencing
bam
nanopore
sequencing
6 hours ago by
njornet
▴ 20
0
votes
3
replies
135
views
Problem with Calling Variants from RNA-Seq data
vcf
gatk
benchmark
giab
rna-seq
1 day ago by
Esraa
• 0
3
votes
3
replies
184
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
updated 3 hours ago by
ATpoint
82k • written 1 day ago by
Alexandra
• 0
1
vote
1
reply
202
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 1 day ago by
dariober
14k • written 2 days ago by
Holly
▴ 10
1
vote
4
replies
249
views
Filtering VCF files based on VAF giving incorrect results
bcftools
GATK
Bioinformatics
picard
VCF
14 hours ago by
Arton
▴ 10
0
votes
0
replies
88
views
ReactomeGSA
ReactomeGSA
1 day ago by
Shaimaa Gamal
▴ 10
0
votes
0
replies
81
views
News:
New course: Manipulation of NGS Data for Genomic and Population Genetics Analyses
KeepLearning
TScourses
1 day ago by
Transmitting Science communication
▴ 70
0
votes
5
replies
264
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 11 hours ago by
GenoMax
142k • written 1 day ago by
chrisk
• 0
0
votes
2
replies
138
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 1 hour ago by
DGTool
▴ 10 • written 1 day ago by
iqra
• 0
0
votes
0
replies
95
views
Why the shape of RCS is contradictory to the p value for nonlinearity.
RCS
ggplot2
ggplot
R
updated 1 day ago by
GenoMax
142k • written 1 day ago by
Dude
• 0
0
votes
0
replies
90
views
NEURON, Brian2, NEST, or PyNN
NEST
NEURON
PyNN
Brian2
1 day ago by
brunofelicianodeomena
• 0
116,974 results • Page
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Recent Votes
Answer: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
A: How to process (seems) Agilent microarrry data?
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame (uORF)"
A: How To Merge Two Fastq.Gz Files?
Answer: Annotating file using bcftools
Answer: Getting differential ChIP-seq peaks between conditions after calling consensus p
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Recent Replies
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
I think you're misunderstanding some concepts here. WGCNA is a framework that defines modules based on how and which genes are correlated …
Comment: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
GenoMax
142k
> most up-to-date build Major build of the genome remains GRCh38. What you are looking at is the latest patch release for that build. Only…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
manuelmourato25
• 0
Thank you @atpoint . My apologies, maybe I should rephrase the question. I read the WGCNA docs already, and for the Pearson correlation tes…
Comment: How to extract cells of different species after mapping with combined genome?
by
GenoMax
142k
Can you confirm that this answer is specific for `singleron` technology and associated bioinformatics software.
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
What OS are you using? Are you able to run `blastn --help` and does it produce the in-line help? > I used many query file.fasta And tho…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
> However, even though 3 biological replicates per condition seems to be the norm in these experiments, many papers discuss that we need at…
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
ATpoint
82k
> I have curiosity, why do you make a desing with interceptor and without contrast matrix? Because for a 2-group comparison you do not nee…
Comment: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
ATpoint
82k
If you have a one-click workflow ready and the necessary computation power to casually run it, then go for it. If it is any greater effort …
Comment: Seurat v5 and how to correctly integrate across multiple experiments
by
Papyrus
★ 2.9k
In my experience integration methods are also often used for different samples/batches across the same technology. The crucial thing is to …
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
by
egascon
• 0
Thank you very much! I have repeated the code adding arrayWeights() and it's works! In this case, I have a desing without intercept and c…
Comment: Is there any way to modify this pie chart ?
by
ohtang7
▴ 40
Thank you, I've tried but I see only some appear in the chart in wrong segments. Could you help me to solve this how ? ![enter image desc…
Comment: perseus software (version 1.5.5)
by
DGTool
▴ 10
If the tool mentioned relates to the Perseus application from the Cox lab ([here][1]), it should be free to download after accepting the li…
Answer: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
DGTool
▴ 10
At least so far to my knowledge, I don't think it would be worth re-mapping to GRCh38.p14, if it is too much trouble or with what you're lo…
Answer: How to extract cells of different species after mapping with combined genome?
by
Tony
• 0
From the marker genes of each cluster, we can determine whether the cluster belongs to human or mouse. Human genes are all capitalized, whi…
Comment: In one PCA plot, can I calculate the percentage of different factors that contri
by
diqixiaoyaoer
▴ 10
Thanks a lot. It is almost what I need but not all. From your script, I can get information that how much degree different samples contri…
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