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519 results • Page
2 of 11
Sort: Votes
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Views
Votes
Replies
2
votes
18
replies
1.6k
views
error when running phg create-maf-vcf to build a built practical haplotype graph (v2)
pangenome
phg_v2
plants
PHG
24 days ago by
b.contreras.moreira
▴ 170
2
votes
3
replies
1.5k
views
Duplicate row name not allowed while reading the file in R
RNA-Seq
deseq2
Differential-Gene-expression
R
updated 16 days ago by
Ram
43k • written 4.9 years ago by
imanbh
• 0
2
votes
10
replies
824
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 29 days ago by
Ram
43k • written 9 weeks ago by
Sky
▴ 10
2
votes
5
replies
1.6k
views
Adding functional annotation and meta-data of MAKER/BRAKER GFF
metadata
functional-annotation
BRAKER
MAKER
updated 24 days ago by
andorjkiss
▴ 40 • written 15 months ago by
javanokendo
▴ 60
2
votes
5
replies
685
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 5 days ago by
Jesse
▴ 740 • written 6 days ago by
Ann
★ 2.4k
2
votes
7
replies
815
views
Removing duplicates
duplicates
ONT
minimap2
updated 7 days ago by
joe
▴ 510 • written 9 days ago by
quentinperriere
• 0
2
votes
4
replies
293
views
Addmetadata to seurat obj
seurat
15 days ago by
synat.keam
▴ 100
2
votes
2
replies
259
views
Removing adapters in all the RNAseq samples regardless if they have or not, is it correct?
fastqc
trimming
adapters
cutadapt
RNAseq
updated 22 days ago by
dsull
★ 5.8k • written 22 days ago by
ev97
▴ 20
2
votes
4
replies
261
views
interpretartion of a vcf file
calling
genotype
ONT
variant
diploid
2 days ago by
samuelkalandarov2002
▴ 10
2
votes
4
replies
316
views
SnpEff annotates coding duplication as intronic?
snpeff
9 days ago by
kirill.zaslavsky
• 0
2
votes
9
replies
820
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 2 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
2
votes
10
replies
3.4k
views
RepeatMasker: createLib() Error
RepeatMasker
updated 9 days ago by
Ram
43k • written 7.4 years ago by
linda
• 0
2
votes
3
replies
254
views
P-value Threshold Consideration in Multi-Sample RNA-Seq Experiment
statistics
RNA-Seq
p-value
updated 23 days ago by
Ram
43k • written 24 days ago by
Netanel
• 0
2
votes
0
replies
205
views
What are good Python models/packages to predict small molecula (SMILES) binding to proteins ?
SMILES
11 days ago by
Alexander
▴ 220
2
votes
5
replies
669
views
samtools mpileup taking too long
Bam
samtools
sam
alignment
updated 20 days ago by
Ram
43k • written 6 weeks ago by
K
• 0
2
votes
6
replies
632
views
Overlapping and merging ChIP-seq peaks
MACS2
HOMER
ChIP-seq
peak-calling
updated 23 days ago by
Ram
43k • written 4 months ago by
zea
• 0
2
votes
1
reply
953
views
How to evaluate the performance of an RNA-seq pipeline
Differential-gene-expression
RNA-Seq
R
DESeq2
updated 16 days ago by
Ram
43k • written 4.0 years ago by
nhaus
▴ 310
1
vote
1
reply
181
views
Do I need to go back and filter my long-reads?
alignment
nanopore
filtering
QC
ONT
updated 11 days ago by
GenoMax
141k • written 11 days ago by
eesiribloom
▴ 80
1
vote
0
replies
539
views
The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
Primers
TRINITY
6 days ago by
mathavanbioinfo
▴ 90
1
vote
2
replies
1.7k
views
Batch effects and MDSPlot in limma
limma
Batch-effect
MDS
Galaxy
updated 9 days ago by
Ram
43k • written 4.8 years ago by
sonayuv
• 0
1
vote
3
replies
205
views
Filter BAM to keep only alignments at an interval, ignoring reads spliced over
samtools
bam
splicing
3 days ago by
WouterDeCoster
47k
1
vote
3
replies
2.5k
views
Problem to remove subset of patients with plink
plink
PED
updated 3 days ago by
chrchang523
10k • written 6.1 years ago by
Ginevra
▴ 10
1
vote
0
replies
149
views
Contradictions in GSEA results
GSEA
Enrichment-analysis
PEA
updated 28 days ago by
Ram
43k • written 28 days ago by
Tolga
▴ 30
1
vote
4
replies
389
views
what is the normal range of htseq-count output for homo sapiens RNA-Seq
Transcriptomics
htseq-count
RNA-Seq
NGS
hisat2
updated 13 days ago by
BioinfGuru
★ 1.7k • written 14 days ago by
Morteza
• 0
1
vote
0
replies
82
views
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
dnds
kaks
VCF
22 hours ago by
rohitsatyam102
▴ 850
1
vote
5
replies
264
views
Downsampling fastq file
downsample
fastq
updated 3 days ago by
Ram
43k • written 3 days ago by
marco.barr
▴ 80
1
vote
1
reply
2.7k
views
Plotting a PCA Plot from SVASeq results
pca
ruvseq
sva
svaseq
batch-effect
updated 9 days ago by
Ram
43k • written 6.2 years ago by
jjrin
▴ 40
1
vote
1
reply
175
views
Failed to download data from EBI with ascp
EBI
aspera
updated 15 days ago by
GenoMax
141k • written 15 days ago by
biock
▴ 60
1
vote
2
replies
209
views
Flow Cytometry Data Analysis by Seurat
Seurat
updated 28 days ago by
Ram
43k • written 29 days ago by
gdfsnkfns
• 0
1
vote
1
reply
462
views
PLINK multivariable GLM output: p-value without BETA and SE
linear-model
glm
plink
updated 17 days ago by
chrchang523
10k • written 18 days ago by
dsbusiness135
▴ 10
1
vote
1
reply
414
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
updated 5 days ago by
Gordon Smyth
★ 7.0k • written 8 days ago by
Chris
▴ 260
1
vote
4
replies
2.8k
views
removing confounding batch effect with RUVseq
RUVseq
RNA-Seq
batch-effect
DESeq2
updated 8 days ago by
Ram
43k • written 5.1 years ago by
grant.hovhannisyan
★ 2.6k
1
vote
3
replies
1.7k
views
Using Nanoplot to asses quality of ONT data
ONT
Long-read-sequencing
NanoPlot
updated 20 days ago by
Ram
43k • written 2.3 years ago by
matt81rd
▴ 10
1
vote
2
replies
287
views
Tumour purity and ploidy estimation
Copy-number
Whole-exome-sequencing
10 days ago by
bp22
▴ 80
1
vote
1
reply
821
views
Question for 3 prime bias in rna short read and long read (illumina, ont, pacbio)
rna-seq
short-read
long-read
updated 20 days ago by
Ram
43k • written 11 months ago by
shinyjj
▴ 50
1
vote
3
replies
6.1k
views
Error for converting from gene symbol to Entrez IDs
Entrez-ID
org.Dr.eg.db
updated 9 days ago by
Ram
43k • written 5.5 years ago by
modarzi
▴ 170
1
vote
4
replies
2.4k
views
Batch effect control in GWAS data.
Batch-effect
omniexpress
GWAS
GSA
updated 8 days ago by
Ram
43k • written 4.8 years ago by
saksis.rihards
▴ 10
1
vote
1
reply
225
views
Retrieve a % coverage for each transcript
RNA-seq
updated 8 days ago by
Ram
43k • written 8 days ago by
jammydodger123456
▴ 40
1
vote
2
replies
315
views
Extracting information from gff3 file produced by augustus
augustus
gff3
updated 10 days ago by
Istvan Albert
100k • written 12 days ago by
Jiang
• 0
1
vote
4
replies
812
views
I want to calculate if the sample has whole genome duplication event or not?
sequencing
wgs
16 days ago by
Hyper_Odin
▴ 310
1
vote
3
replies
255
views
CCA with random effects model
CCA
updated 24 days ago by
dariober
14k • written 24 days ago by
leranwangcs
▴ 120
1
vote
1
reply
179
views
Help/Direction Needed for Data Analysis of RNA
Sequencing
RNA-seq
DNA-Seq
updated 9 days ago by
dsull
★ 5.8k • written 9 days ago by
niruf
• 0
1
vote
3
replies
248
views
some error in building kraken2 database
metagenome
kraken2
2 days ago by
Art1ess
• 0
1
vote
2
replies
327
views
BAM to CRAM and BAM recover with smaller size
BAM
samtools
CRAM
updated 23 days ago by
jkbonfield
★ 1.2k • written 5 weeks ago by
geocarvalho
▴ 360
1
vote
6
replies
1.6k
views
How can Differentially Expressed Genes be found by limma package after removing batch effect by ComBat function in sva package?
limma
sva
batch-effect
R
updated 10 days ago by
Ram
43k • written 3.8 years ago by
amirmehrgou
▴ 10
1
vote
0
replies
47
views
Simulation of label-free bottom-up proteomics expression dataset
label-free
bottom-up
lc-ms
proteomics
8 hours ago by
KABILAN
▴ 50
1
vote
0
replies
155
views
DESeq2 comparing timepoints within treatment and account for background control change
RNAseq
DEG
DESeq2
updated 14 days ago by
Ram
43k • written 15 days ago by
ian.will
▴ 30
1
vote
2
replies
922
views
vg surject for long reads
long-reads
vg
variation-graph
surject
updated 20 days ago by
Ram
43k • written 23 months ago by
ved_vyas
▴ 10
1
vote
0
replies
368
views
Differential expression analysis on multiple integrated datasets
microarray
Z-score
limma
differential-gene-expression
meta-analysis
updated 16 days ago by
Ram
43k • written 2.4 years ago by
michael.s
▴ 10
1
vote
3
replies
347
views
Ensembl gene id conversion
biomart
ensembl
updated 10 days ago by
ATpoint
82k • written 12 days ago by
naveedhasan2000
• 0
519 results • Page
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Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
cmdcolin
★ 3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq
by
pinheirofabiano
▴ 10
install.packages("pheatmap") library(pheatmap) setwd("/Users/data_analysis/results") data <- read.table(file…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
bedtools intersect \ -a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \ -b <(awk '{X=250000;P=int($2);printf("%s\t…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
• 0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question
by
starswillfade
▴ 10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
Comment: ScRNA data question
by
starswillfade
▴ 10
![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
Comment: genome assembly records not present in assembly_summary.txt
by
GenoMax
141k
Can you clarify what `metainfo` you are referring to? The accession that you refer to does exist in [**GenBank assembly_summary file**][1…
Comment: what is the purpose of indexing the reference genome (Kallisto)
by
i.sudbery
19k
Specifically: If we want to look up all the places where a 31mer is in a genome without and index, we have to compare that 31 to the first…
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
benformatics
3.9k
This is way easier to do solely in R now 10 years later. library(GenomicFeatures) library(rtracklayer) ## import GTF…
Answer: what is the purpose of indexing the reference genome (Kallisto)
by
dsull
★ 5.8k
I'll see if I can explain things in super simple terms (albeit with some abuse of the actual technical details) to provide a conceptual und…
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
Jalil Sharif
▴ 80
The updated code in R using rtracklayer for reading a gtf file. https://github.com/jalilsharif/gtf_utr_fix_r/blob/main/gencode_utr_fix_d…
Comment: Rare Disease Variant Pathway Analysis
by
The_PyPanda
▴ 10
Thank you LauferVA, Your advice has been useful and I now a lot to think about and read. Also, I just wanted to clarify I do not ha…
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