updated 3 months ago by
Biostar
2.7k
•
written 7.4 years ago by
Istvan Albert
100k
795
votes
167
replies
143k
views
109 follow
122
votes
44
replies
11k
views
24 follow
updated 6 months ago by
Ram
43k
•
written 11.5 years ago by
Istvan Albert
100k
46
votes
16
replies
5.9k
views
12 follow
updated 11 months ago by
Ram
43k
•
written 6.4 years ago by
Hussain Ather
▴
990
38
votes
7
replies
2.3k
views
38
votes
8
replies
1.6k
views
6 follow
updated 10 months ago by
Alex Reynolds
35k
•
written 10 months ago by
David Langenberger
11k
24
votes
21
replies
7.6k
views
9 follow
14
votes
3
replies
3.7k
views
updated 11 months ago by
Ram
43k
•
written 6.0 years ago by
lakhujanivijay
5.8k
14
votes
11
replies
3.0k
views
6 follow
updated 11 months ago by
Ram
43k
•
written 6.4 years ago by
anicet.ebou
▴
170
13
votes
2
replies
1.5k
views
News:
Thank you Biostars!
updated 11 months ago by
Ram
43k
•
written 5.0 years ago by
Glory Basumata
▴
140
13
votes
9
replies
2.7k
views
7 follow
12
votes
4
replies
2.0k
views
updated 11 months ago by
Ram
43k
•
written 5.9 years ago by
kzkedzierska
▴
290
12
votes
2
replies
495
views
11
votes
11
replies
1.8k
views
8 follow
updated 11 months ago by
Ram
43k
•
written 5.5 years ago by
carlopecoraro2
★
2.5k
11
votes
9
replies
7.7k
views
News:
NovaSeq quality analysis
updated 12 months ago by
Ram
43k
•
written 6.9 years ago by
Brian Bushnell
20k
10
votes
4
replies
1.8k
views
updated 11 months ago by
Ram
43k
•
written 5.3 years ago by
David Langenberger
11k
10
votes
0
replies
2.1k
views
9
votes
3
replies
4.8k
views
9
votes
4
replies
2.5k
views
7 follow
9
votes
4
replies
1.6k
views
8
votes
6
replies
17k
views
updated 11 months ago by
Ram
43k
•
written 6.4 years ago by
Ahmed Youssef
▴
70
8
votes
8
replies
2.9k
views
updated 11 months ago by
Ram
43k
•
written 4.2 years ago by
Istvan Albert
100k
8
votes
0
replies
1.3k
views
updated 11 months ago by
Ram
43k
•
written 4.9 years ago by
David Langenberger
11k
8
votes
2
replies
2.4k
views
8
votes
2
replies
828
views
8
votes
6
replies
1.5k
views
updated 11 months ago by
Ram
43k
•
written 5.5 years ago by
Istvan Albert
100k
8
votes
4
replies
2.0k
views
updated 11 months ago by
Ram
43k
•
written 4.6 years ago by
Erik Garrison
★
2.4k
7
votes
2
replies
1.2k
views
3 months ago by
David Langenberger
11k
7
votes
0
replies
1.0k
views
7
votes
5
replies
2.6k
views
7
votes
3
replies
3.9k
views
7
votes
8
replies
1.9k
views
updated 11 months ago by
Ram
43k
•
written 6.4 years ago by
tiagoantao
▴
690
7
votes
11
replies
2.0k
views
updated 12 months ago by
Ram
43k
•
written 6.8 years ago by
Paul Kutzner
▴
100
6
votes
7
replies
2.2k
views
6
votes
1
reply
2.9k
views
updated 11 months ago by
Ram
43k
•
written 5.0 years ago by
Pierre Lindenbaum
161k
6
votes
13
replies
2.1k
views
6
votes
4
replies
1.3k
views
6
votes
2
replies
2.1k
views
updated 12 months ago by
Ram
43k
•
written 6.8 years ago by
David Langenberger
11k
6
votes
0
replies
977
views
updated 11 months ago by
Ram
43k
•
written 4.6 years ago by
Michael R. Crusoe
★
1.9k
6
votes
2
replies
1.1k
views
updated 11 months ago by
Ram
43k
•
written 5.3 years ago by
Ben_Ensembl
★
2.4k
6
votes
0
replies
2.3k
views
6
votes
0
replies
1.5k
views
updated 12 months ago by
Ram
43k
•
written 6.8 years ago by
patriciareneecarter
▴
90
6
votes
1
reply
1.0k
views
updated 11 months ago by
Ram
43k
•
written 6.4 years ago by
Elisabeth Gasteiger
★
2.4k
5
votes
1
reply
1.6k
views
updated 11 months ago by
Ram
43k
•
written 5.9 years ago by
carlopecoraro2
★
2.5k
5
votes
2
replies
929
views
updated 11 months ago by
Ram
43k
•
written 4.8 years ago by
hollystephens723
▴
70
5
votes
0
replies
1.8k
views
updated 11 months ago by
Ram
43k
•
written 5.4 years ago by
dashabalashova
▴
50
5
votes
2
replies
1.7k
views
5
votes
2
replies
1.5k
views
News:
Google Dataset Search
updated 11 months ago by
Ram
43k
•
written 5.7 years ago by
Pierre Lindenbaum
161k
5
votes
3
replies
1.5k
views
updated 11 months ago by
Ram
43k
•
written 5.3 years ago by
field.cady
▴
50
5
votes
0
replies
1.5k
views
Recent Votes
Recent Locations •
All
UCLA, just now
France, 1 minute ago
Canada, 2 minutes ago
United States, 2 minutes ago
France/Nantes/Institut du Thorax - INSERM UMR1087, 8 minutes ago
United States, 9 minutes ago
United Kingdom, 10 minutes ago
Recent Awards •
All
Popular Question to
Lélé
•
0
Commentator to
dthorbur
★
1.9k
Popular Question to
maria.soler
•
0
Popular Question to
melissachua90
▴
70
Popular Question to
Lila M
★
1.2k
Popular Question to
Amr
▴
160
Commentator to
rpolicastro
13k
Recent Replies
Comment: cellranger error message
by
Max
•
0
I think the problem is your fastq_id. This should also read "RabhiN_PHLGEX". Hope this helps!
Comment: How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
by
Giulio Genovese
▴
390
@yokofakun is correct. Also, do notice that the GATK option `--RECOVER_SWAPPED_REF_ALT True` does not work with indels. In general, if your…
Comment: How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
by
Pierre Lindenbaum
161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
by
Pierre Lindenbaum
161k
I think this is useless, in the gatk doc:
> For each variant, the tool will look for the target coordinate, reverse-complement and left-al…
Comment: Too many unpaired forward reads found by Trimmomatic
by
GenoMax
142k
Ideally you will know which adapters to use but if you don't then programs like `fastp` can auto-detect them. You could also use `bbduk.sh`…
Comment: UMI-Tools knee-method has great influence on the results of white list
by
i.sudbery
19k
With the `--read2-only` option (which isn't available yet on the bioconda version), this will complain about a lack of `--bc-pattern`.
How…
Comment: RNA seq analysis
by
prifa
▴
10
it's raw data **fastq.gz**, so for example I have 4 different samples from different tissue, sample A with 3 replicates, samples B,C and D …
Answer: Filtering Multi-sample VCF file for all except one Genotype
by
Pierre Lindenbaum
161k
> The goal is to remove all the variants which are unique to the KWSBambina sample.
using jvarkit vcffilterjdk https://jvarkit.readthedo…
Comment: Too many unpaired forward reads found by Trimmomatic
by
SilhouetteQ
•
0
The first example used the adapter file NexteraPE-PE.fa, but it generated more than 50% unpaired data......
The content of NexteraPE-PE.fa…
Comment: Filtering Multi-sample VCF file for all except one Genotype
by
schmince
•
0
Ok so I definitely didn't understand at first what my goal was.
The goal is to remove all the variants which are unique to the KWSBambina…
Comment: Question about methylation location
by
dthorbur
★
1.9k
What data do you have? Generally, you start with sequencing data, which you need to clean and then map to a reference genome. Then dependin…
Comment: Too many unpaired forward reads found by Trimmomatic
by
GenoMax
142k
> when I didn't provide the adapter file
Still see the file here `ILLUMINACLIP:./NexteraPE-PE.fa:2:30:10 `, unless that is an empty file.
Comment: Too many unpaired forward reads found by Trimmomatic
by
SilhouetteQ
•
0
Interestingly, when I didn't provide the adapter file, the number of unpaired forward reads was significantly reduced:
TrimmomaticPE…
Comment: Alignment of samples with spike-in
by
GenoMax
142k
> but then if you only allow to map uniquely mapped reads, you are losing spike-in information,
How so? Spike-in's are generally unique s…
Traffic: 2934 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6