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Showing :
questions
0
votes
2
replies
624
views
Phylotranscriptomics approach understanding evolution from museum specimens ?
genomics
RNA
alignment
3.1 years ago by
sunnykevin97
▴ 980
0
votes
1
reply
747
views
BlastN - Optimal recipocal blast command line options ?
alignment
blastn
updated 3.1 years ago by
lieven.sterck
15k • written 3.1 years ago by
sunnykevin97
▴ 980
4
votes
2
replies
951
views
Finding genes under positive selection in non-model species - Phylotranscriptomics approach ?
gene
ortholog
assembly
rna-seq
updated 5 months ago by
Ram
43k • written 3.1 years ago by
sunnykevin97
▴ 980
6
votes
3
replies
1.2k
views
Running blastn to get best hits match using (-max_target_seqs 1 -max_hsps 1 ) ?
blast
alignment
3.1 years ago by
sunnykevin97
▴ 980
10
votes
3
replies
2.8k
views
Blast hits output to fasta format ?
alignment
assembly
blast
updated 3.1 years ago by
5heikki
11k • written 3.1 years ago by
sunnykevin97
▴ 980
2
votes
1
reply
728
views
Converting Aminoacid MSA alignment to Nucleotide MSA alignment ?
nucleotide
dna
alignment
protein
rna
updated 3.1 years ago by
Mensur Dlakic
★ 27k • written 3.1 years ago by
sunnykevin97
▴ 980
2
votes
6
replies
1.5k
views
How to find orthologus sequences between the different species of non-model organisms?
RNA-Seq
Transcript
Assembly
ORF
3.1 years ago by
sunnykevin97
▴ 980
0
votes
5
replies
2.0k
views
bam2cfg.pl not able to find statistics : Breakdancer
gen
Assembly
software error
updated 3.1 years ago by
Biostar
20 • written 5.6 years ago by
sunnykevin97
▴ 980
2
votes
2
replies
1.8k
views
not able to run Fastqc in Centos server
fastqc
genomics
updated 3.2 years ago by
Biostar
20 • written 4.4 years ago by
sunnykevin97
▴ 980
2
votes
3
replies
737
views
What are the reasons to find so few Differentially expressed genes (DEGs)?
RNA-Seq
updated 8 months ago by
Manuel Sokolov Ravasqueira
▴ 100 • written 3.3 years ago by
sunnykevin97
▴ 980
1
vote
5
replies
2.0k
views
Which pipeline is the best for differentinal gene expression analysis (edgeR, DESEQ2 or HISAT2 - stringtie - ballgown)?
RNA-Seq
updated 3.6 years ago by
Manuel Mendoza
▴ 10 • written 3.6 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
389
views
Is their any tool tell us about housekeeping genes ?
RNA-Seq
3.6 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
1.1k
views
PCA using ggplot2/ggfortify autoplot function ?
RNA-Seq
3.6 years ago by
sunnykevin97
▴ 980
5
votes
3
replies
986
views
Using glMDPlot function how to show the DGE's in color ?
RNA-Seq
3.6 years ago by
sunnykevin97
▴ 980
5
votes
5
replies
683
views
How to make a connection b/w behavioral data Vs. gene expression data ?
RNA-Seq
updated 3.6 years ago by
Kevin Blighe
87k • written 3.6 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
549
views
How do I compare ortholog protein sequences using domain information ?
RNA-Seq
protein
3.6 years ago by
sunnykevin97
▴ 980
8
votes
6
replies
1.5k
views
rnaQUAST-2.1.0/BUSCO, blast-2.10.0 multithreading problem?
RNA-Seq
updated 3.6 years ago by
h.mon
35k • written 3.6 years ago by
sunnykevin97
▴ 980
5
votes
3
replies
770
views
The gene names ending with *like suffix ?
RNA-Seq
3.6 years ago by
sunnykevin97
▴ 980
7
votes
4
replies
1.7k
views
How to sum the genewise counts in edgeR ?
RNA-Seq
r
R
updated 3.7 years ago by
rpolicastro
13k • written 3.7 years ago by
sunnykevin97
▴ 980
0
votes
3
replies
744
views
Voom Unormalised vs Normalised logCPM ?
RNA-Seq
3.8 years ago by
sunnykevin97
▴ 980
1
vote
1
reply
3.9k
views
How to Interpreting voom : Mean-variance trend ?
RNA-Seq
limma
voom
updated 3.8 years ago by
h.mon
35k • written 3.8 years ago by
sunnykevin97
▴ 980
3
votes
11
replies
3.9k
views
how to format the featurecounts output ?
rna
RNA-Seq
3.8 years ago by
sunnykevin97
▴ 980
4
votes
8
replies
1.6k
views
looping through PE files ?
RNA-Seq
updated 3.8 years ago by
antonioggsousa
3.2k • written 3.8 years ago by
sunnykevin97
▴ 980
3
votes
5
replies
3.6k
views
How to generate dotplot for gene expression data differential gene expression analysis ?
RNA-Seq
updated 3.8 years ago by
antonioggsousa
3.2k • written 3.8 years ago by
sunnykevin97
▴ 980
1
vote
1
reply
590
views
Gene ontology for differential genes (easy manual)?
RNA-Seq
annotation
KEGG
updated 3.8 years ago by
jared.andrews07
★ 16k • written 3.8 years ago by
sunnykevin97
▴ 980
0
votes
1
reply
564
views
Kallisto -s parameter ?
RNA-Seq
3.9 years ago by
sunnykevin97
▴ 980
1
vote
11
replies
2.1k
views
How to make a gene counts matrix ? (input edgeR/DESeq2)
RNA-Seq
rna-seq
Assembly
gene
updated 3.9 years ago by
Alex Nesmelov
▴ 200 • written 3.9 years ago by
sunnykevin97
▴ 980
2
votes
1
reply
519
views
Differential gene expression analysis ?
RNA-Seq
assembly
updated 3.9 years ago by
Kevin Blighe
87k • written 3.9 years ago by
sunnykevin97
▴ 980
7
votes
4
replies
1.7k
views
How important is normalization for RNA_Seq data ?
RNA-Seq
assembly
R
updated 3.9 years ago by
i.sudbery
19k • written 3.9 years ago by
sunnykevin97
▴ 980
1
vote
3
replies
952
views
Transcript quantification/Differential gene expression analysis ?
RNA-Seq
Assembly
updated 3.9 years ago by
h.mon
35k • written 3.9 years ago by
sunnykevin97
▴ 980
2
votes
4
replies
863
views
How to start Differential gene expression analysis ?
RNA-Seq
updated 3.9 years ago by
ATpoint
82k • written 3.9 years ago by
sunnykevin97
▴ 980
1
vote
3
replies
829
views
KEGG mapping for larger sample size (100) ?
RNA-Seq
KEGG
updated 3.9 years ago by
young_bioinformatician
▴ 230 • written 3.9 years ago by
sunnykevin97
▴ 980
0
votes
2
replies
1.2k
views
How to generate GTF file ?
RNA-Seq
3.9 years ago by
sunnykevin97
▴ 980
0
votes
2
replies
529
views
Unable to run Trans-ABySS on RNA_seq data?
assembly
RNA-Seq
updated 4.0 years ago by
Biostar
20 • written 4.1 years ago by
sunnykevin97
▴ 980
1
vote
3
replies
1.1k
views
Cutdapt for batch processing ?
genome
RNA-Seq
sequencing
updated 4.2 years ago by
Asaf
10k • written 4.2 years ago by
sunnykevin97
▴ 980
4
votes
7
replies
3.4k
views
MultiQC not generating ouput ?
next-gen
rna-seq
updated 4.2 years ago by
ATpoint
82k • written 4.2 years ago by
sunnykevin97
▴ 980
13
votes
5
replies
1.5k
views
Difference b/w primary and alternate genome assembly ?
genome
Assembly
updated 4.3 years ago by
GenoMax
142k • written 4.3 years ago by
sunnykevin97
▴ 980
3
votes
1
reply
862
views
Trinity showing up fastq entry error ?
RNA-Seq
Denovo assembly
updated 4.4 years ago by
h.mon
35k • written 4.4 years ago by
sunnykevin97
▴ 980
0
votes
2
replies
1.4k
views
How to combine and convert fastq PE files into fasta files as batch?
fastq
fasta
denovoassembly
updated 4.4 years ago by
ashish
▴ 680 • written 4.4 years ago by
sunnykevin97
▴ 980
0
votes
4
replies
822
views
How to construct Pantranscriptome from RNA_Seq data ?
RNA-Seq
pantgenomics
pantranscriptomics
updated 4.4 years ago by
JC
13k • written 4.4 years ago by
sunnykevin97
▴ 980
6
votes
7
replies
4.3k
views
how to extract coding sequences (CDS) from a bam file or vcf file ?
alignment
SNP
updated 4.6 years ago by
anna11
▴ 20 • written 5.3 years ago by
sunnykevin97
▴ 980
1
vote
16
replies
7.0k
views
After sorting bam file not able to index ?
alignment
next-gen
updated 5.2 years ago by
Biostar
20 • written 5.3 years ago by
sunnykevin97
▴ 980
0
votes
0
replies
1.2k
views
How to perform Null and Alternative models using PAML (estimation of dN/dS) ?
alignment
snp
sequence
5.3 years ago by
sunnykevin97
▴ 980
1
vote
2
replies
2.3k
views
how to convert a fasta file to a PAML input file (nuc format) ?
alignment
SNP
5.3 years ago by
sunnykevin97
▴ 980
0
votes
3
replies
1.0k
views
Multiple sequence alignment tools for bigdata genomics ?
genome
alignment
5.3 years ago by
sunnykevin97
▴ 980
4
votes
6
replies
2.2k
views
Running PAML without Tree file?
sequence
alignment
updated 5.3 years ago by
Joe
21k • written 5.3 years ago by
sunnykevin97
▴ 980
0
votes
3
replies
1.9k
views
vcf tools (vcf-stats) not able to generate stats ?
alignment
sequence
SNP
5.3 years ago by
sunnykevin97
▴ 980
16
votes
10
replies
7.4k
views
How to split a multi fasta file into individual chromosomes
alignment
sequence
updated 5.3 years ago by
finswimmer
16k • written 5.3 years ago by
sunnykevin97
▴ 980
0
votes
2
replies
1.4k
views
MUGSY not able to perform alignment ?
alignment
snp
updated 5.4 years ago by
WouterDeCoster
47k • written 5.4 years ago by
sunnykevin97
▴ 980
2
votes
2
replies
2.3k
views
What is the best tool for generating Multiple sequence alignment (MSA) of fasta sequences ?
sequence
alignment
updated 5.4 years ago by
Jean-Karim Heriche
27k • written 5.4 years ago by
sunnykevin97
▴ 980
107 results • Page
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