updated 3 days ago by
cwwong13
▴
40
•
written 6.7 years ago by
lessismore
★
1.3k
40
votes
10
replies
42k
views
8 follow
9
votes
10
replies
6.9k
views
1
vote
10
replies
4.0k
views
9
votes
10
replies
403
views
6 follow
updated 6 hours ago by
Zamin Iqbal
▴
20
•
written 1 day ago by
nicole.kavanagh
•
0
0
votes
10
replies
483
views
2
votes
10
replies
3.4k
views
8
votes
10
replies
750
views
9
votes
10
replies
4.4k
views
updated 15 days ago by
Ram
43k
•
written 7.4 years ago by
denalitastic
▴
30
1
vote
10
replies
972
views
8 days ago by
DKA
▴
40
6
votes
10
replies
1.7k
views
7
votes
10
replies
2.7k
views
6 follow
3
votes
10
replies
8.5k
views
6 follow
6
votes
10
replies
2.3k
views
4
votes
10
replies
1.5k
views
6
votes
10
replies
1.9k
views
13
votes
10
replies
6.4k
views
11
votes
9
replies
11k
views
10 follow
2
votes
9
replies
865
views
1
vote
9
replies
4.7k
views
0
votes
9
replies
2.5k
views
6 follow
11
votes
9
replies
7.0k
views
0
votes
9
replies
613
views
1
vote
9
replies
554
views
27 days ago by
Assa Yeroslaviz
★
1.8k
4
votes
9
replies
2.1k
views
3 days ago by
anna
▴
20
3
votes
9
replies
496
views
updated 8 days ago by
Istvan Albert
100k
•
written 10 days ago by
Κοσμάς
•
0
2
votes
9
replies
567
views
updated 28 days ago by
Pierre Lindenbaum
161k
•
written 29 days ago by
emilydolivo97
▴
10
3
votes
9
replies
874
views
updated 14 days ago by
Elisabeth Gasteiger
★
2.4k
•
written 14 days ago by
Jacky
•
0
1
vote
9
replies
1.2k
views
4
votes
9
replies
593
views
updated 29 days ago by
Ram
43k
•
written 4 weeks ago by
dalibenam64
•
0
4
votes
9
replies
2.7k
views
Tool:
CANEapp, a user-friendly application for automated next generation transcriptomic data analysis
written 8.3 years ago by
dvelmeshev
•
0
0
votes
9
replies
1.2k
views
5
votes
8
replies
3.7k
views
7
votes
8
replies
681
views
3
votes
8
replies
4.9k
views
1
vote
8
replies
852
views
updated 7 days ago by
Pierre Lindenbaum
161k
•
written 14 days ago by
Maria
•
0
6
votes
8
replies
592
views
updated 15 days ago by
dariober
14k
•
written 17 days ago by
manaswiniparija3
▴
40
4
votes
8
replies
637
views
0
votes
8
replies
5.3k
views
8 follow
1
vote
8
replies
439
views
2 days ago by
marco.barr
▴
90
17
votes
8
replies
13k
views
9 follow
updated 29 days ago by
AshleeThomson
▴
80
•
written 6.1 years ago by
lauren.wahyudi
▴
50
8
votes
8
replies
624
views
3
votes
8
replies
6.4k
views
7 follow
updated 3 hours ago by
Sd
•
0
•
written 5.1 years ago by
Nicolas Rosewick
11k
6
votes
8
replies
2.1k
views
updated 24 days ago by
mflab
•
0
•
written 3.9 years ago by
m.sadman.sakib
▴
120
0
votes
8
replies
419
views
3
votes
8
replies
1.3k
views
3
votes
8
replies
4.1k
views
0
votes
8
replies
573
views
updated 27 days ago by
Michael
54k
•
written 28 days ago by
emilydolivo97
▴
10
1
vote
8
replies
3.5k
views
0
votes
8
replies
364
views
Recent Votes
Recent Locations •
All
United States, just now
Australia, 2 minutes ago
Philippines, 3 minutes ago
Berlin, 4 minutes ago
United States, 5 minutes ago
United States, 5 minutes ago
San Francisco, 6 minutes ago
Recent Awards •
All
Popular Question to
Buffo
★
2.4k
Popular Question to
yvonnehhe
•
0
Popular Question to
curious
▴
750
Scholar to
jared.andrews07
★
16k
Popular Question to
hafiz.talhamalik
▴
350
Popular Question to
Hayler Edu
▴
40
Popular Question to
eesiribloom
▴
80
Recent Replies
Answer: Bedtools merge minimum overlap?
by
harold.smith.tarheel
★
4.9k
Bedtools [intersect][1] allows you to specify the fraction of overlap between two BED (or BAM) files using the F/f/r flags. You could split…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴
40
Sorry, I should have been more specific. By "that list" I mean what's populated in the Therapeutic Agents tab when no filters are applied. …
Comment: Bedtools merge minimum overlap?
by
bk11
★
2.4k
Not sure if you wanting to do like this-
cat your_input.bed
chr1 100 200 region1 +
chr1 180 300 regi…
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
If you know what the boundaries of your construct look like then trim the left-end of the read using the tag on that end (`ktrim=l`). Then …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> Not every read in your data is going to match a guide.
That is also true for sure. Let me clarify what I mean. For every read you should…
Answer: VG : No reference-sense paths available in the graph; falling back to generic pa
by
anovak
▴
120
If your GFA has paths in it that are P lines with names that don't include a sample name, contig name, and separators, then those are what …
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
> if you look at a fastq file you should be able to tell for every read definitely, if it has a perfect match for a guide in it and what gu…
Answer: DiffBind: no peaks in DBA
by
jared.andrews07
★
16k
Because `dba.analyze` is not meant to be run directly on a peaks file. Have you read the documentation?
In addition, a ChIP-seq experiment…
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as yo…
Comment: Converting CRAM to FastQ
by
GenoMax
142k
Use `samtools view ` for this conversion. See discussion in https://www.biostars.org/p/9592860/
Specifically @jkbonfield's comment here --…
Comment: How to access TCGA samples that were treated with a specific drug?
by
GenoMax
142k
No that was only from Breast cancer. You could try selecting all data and see if you are able to see all treatments in the set.
No idea a…
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as you …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> Can you try lowering the k to k=9?
Wouldn't that mess with the results since all my kmers are 20bp?
> You don't have any duplicate sequ…
Comment: Provean help
by
Mensur Dlakic
★
27k
> Else, I will happy to send over the data and kindly perform the analysis for me.
This website is meant to provide advice, not service. I…
Traffic: 2541 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6