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110,335 results • Page
2 of 2207
Sort: Views
Rank
Views
Votes
Replies
43
votes
15
replies
86k
views
9 follow
How Do I Count The Number Values Less Than X In A Column Using R ?
rna
edger
r
counts
updated 8.5 years ago by
seidel
11k • written 12.1 years ago by
Jason
▴ 920
238
votes
176
replies
85k
views
73 follow
Tutorial:
Survival analysis of TCGA patients integrating gene expression (RNASeq) data
RNA-Seq
Survival
TCGA
updated 6 days ago by
Ram
39k • written 7.8 years ago by
TriS
★ 4.6k
101
votes
31
replies
84k
views
18 follow
Best Way To Merge A Many Thousand Small Bam Files Into One Big Bam File?
bam
picard
samtools
merge
updated 10 months ago by
Ram
39k • written 11.9 years ago by
2184687-1231-83-
★ 5.0k
79
votes
22
replies
83k
views
16 follow
How To Split One Big Sequence File Into Multiple Files With Less Than 1000 Sequences In A Single File
next-gen
sequencing
fasta
rna
r
perl
updated 6 months ago by
Ram
39k • written 11.6 years ago by
Hamilton
▴ 290
55
votes
11
replies
82k
views
8 follow
merge large amount of fastq files into a single one
ChIP-Seq
fastq
updated 12 months ago by
Ram
39k • written 8.2 years ago by
catherine
▴ 220
106
votes
45
replies
81k
views
30 follow
How To Extract A Sequence From A Big (6Gb) Multifasta File ?
fasta
updated 9 months ago by
aswinssoman
▴ 30 • written 10.9 years ago by
Mchimich
▴ 320
70
votes
20
replies
81k
views
15 follow
How To Get Ensembl Id (Gene, Transcript, Protein) Mapping Information?
ensembl
identifiers
mapping
updated 4 months ago by
Ram
39k • written 12.6 years ago by
Unode
▴ 180
185
votes
43
replies
79k
views
29 follow
Drawing Chromosome Ideograms With Data
chromosome
visualization
ideogram
updated 8 months ago by
Ram
39k • written 13.2 years ago by
Yuri
★ 1.6k
120
votes
8
replies
78k
views
GRCh37/38(NCBI) vs hg19/hg38(UCSC)
ncbi
ucsc
grch38
hg38
updated 17 months ago by
Ram
39k • written 8.7 years ago by
pwg46
▴ 520
136
votes
25
replies
77k
views
14 follow
Tutorial:
Piping With Samtools, Bwa And Bedtools
unix
samtools
bwa
bedtools
updated 14 months ago by
Ram
39k • written 11.1 years ago by
Ying W
★ 4.2k
33
votes
4
replies
77k
views
7 follow
Merging Bam Files
picard
samtools
updated 4.0 years ago by
abhijit.synl
▴ 60 • written 11.4 years ago by
Tryingtogetthere
▴ 180
95
votes
27
replies
77k
views
21 follow
Extract Sub-Set Of Regions From Vcf File
vcf
tabix
genome
filter
updated 11 days ago by
NIRJHAR
• 0 • written 11.0 years ago by
Rubal7
▴ 820
126
votes
24
replies
76k
views
18 follow
Sequence Length Distribution From A Fastq File
sequence
length
fastq
updated 4.1 years ago by
LuckyLuck
• 0 • written 10.0 years ago by
deepthithomaskannan
▴ 360
160
votes
62
replies
76k
views
37 follow
Selecting Random Pairs From Fastq?
random
fastq
sequence
illumina
code
updated 11 months ago by
erin.baschal
• 0 • written 12.2 years ago by
Ketil
4.1k
99
votes
16
replies
75k
views
15 follow
How Much Coverage Do We Need For An Rna-Seq Experiment?
rna-seq
coverage
8.5 years ago by
Obi Griffith
20k
17
votes
12
replies
75k
views
8 follow
BLAST Database error: No alias or index file found for nucleotide database
blast
blastn
sift
makeblastdb
updated 9 months ago by
Luke
• 0 • written 8.6 years ago by
arronslacey
▴ 320
82
votes
21
replies
74k
views
13 follow
Tutorial:
How to Install SamTools, HTSLib, and BCFTools on Ubuntu 18.04
bcftools
samtools
htslib
updated 3 months ago by
cmdcolin
★ 3.0k • written 4.8 years ago by
otwtgin2010
▴ 540
103
votes
12
replies
73k
views
7 follow
Bedtools Compare Multiple Bed Files?
bedtools
intersect
updated 7 months ago by
Ram
39k • written 11.6 years ago by
Bioscientist
★ 1.7k
20
votes
2
replies
72k
views
Subsetting in R based on row name?
R
updated 7.1 years ago by
Devon Ryan
103k • written 7.1 years ago by
hakimelakhrass
▴ 80
122
votes
17
replies
71k
views
8 follow
What Are Phased And Unphased Genotypes?
genotyping
genome
updated 14 months ago by
Ram
39k • written 12.1 years ago by
Nick
▴ 370
43
votes
25
replies
70k
views
10 follow
Will A Masters In Bioinformatics Help Me In Getting A Job?
career
education
updated 12.2 years ago by
Eric Fournier
★ 1.4k • written 12.2 years ago by
Bioinfo
▴ 50
22
votes
2
replies
70k
views
How Can I Make A Bed File?
bed
updated 11.7 years ago by
John M
▴ 260 • written 11.7 years ago by
Omid
▴ 580
67
votes
18
replies
70k
views
17 follow
Project For A Beginner Bioinformatics Student
project
java
updated 10 months ago by
Ram
39k • written 11.5 years ago by
And
▴ 230
235
votes
45
replies
69k
views
34 follow
What Is The Best Pipeline For Human Whole Exome Sequencing?
next-gen
sequencing
illumina
exome
human
updated 12.0 years ago by
User 9126
▴ 50 • written 13.0 years ago by
Biomed
4.9k
33
votes
17
replies
69k
views
14 follow
How To Draw A Heat Map For Gene Expression Data?
microarray
heatmap
updated 4 months ago by
Ram
39k • written 12.0 years ago by
Fahmida
▴ 80
59
votes
15
replies
69k
views
9 follow
BAM/SAM to FASTA conversion
sam
bam
updated 13 months ago by
Ram
39k • written 8.3 years ago by
biolab
★ 1.4k
42
votes
14
replies
69k
views
9 follow
Synonymous And Non-Synonymous Snps
snp
updated 13 months ago by
Ram
39k • written 12.4 years ago by
Elena
▴ 250
196
votes
44
replies
69k
views
33 follow
Tutorial:
Analysing Microarray Data In Bioconductor
microarray-analysis
bioconductor
updated 4 months ago by
Ram
39k • written 10.7 years ago by
Obi Griffith
20k
188
votes
50
replies
68k
views
41 follow
How To Remove The Same Sequences In The Fasta Files?
fasta
sequence
duplicates
updated 8 months ago by
Ram
39k • written 12.6 years ago by
Zhangleisdau
▴ 340
138
votes
21
replies
68k
views
18 follow
Database Of Tumor Suppressors And/Or Oncogenes
cancer
gene
database
oncogene
tumor
2.6 years ago by
Malachi Griffith
19k
54
votes
10
replies
68k
views
6 follow
Fold change - a final explanation
RNA-Seq
updated 2.7 years ago by
naloy
• 0 • written 4.6 years ago by
Sebastian Hesse
▴ 320
24
votes
23
replies
67k
views
7 follow
Multiple Histograms In One Plot
r
updated 17 months ago by
Ram
39k • written 9.9 years ago by
Assa Yeroslaviz
★ 1.8k
1
vote
2
replies
67k
views
"SyntaxError: unexpected EOF while parsing " IN PYTHON 3.7
PYTHON
updated 2.2 years ago by
Istvan Albert
98k • written 3.1 years ago by
geethus2009
• 0
51
votes
18
replies
66k
views
10 follow
Extract User Defined Region From An Fasta File
sequence
fasta
updated 5.8 years ago by
tuomastik
▴ 30 • written 11.9 years ago by
Palu
▴ 290
19
votes
8
replies
66k
views
6 follow
Pheatmap Annotation
r
heatmap
updated 7.8 years ago by
wm
▴ 550 • written 10.2 years ago by
James
▴ 80
40
votes
15
replies
66k
views
14 follow
Converting Affymetrix Probes To Gene Ids
affymetrix
conversion
entrez
updated 7 months ago by
Ram
39k • written 9.9 years ago by
Josh
▴ 140
74
votes
13
replies
65k
views
10 follow
Tutorial:
Machine Learning For Prediction of Relapse in Cancer - Part 2 - Building A Random Forest Classifier
r
cancer
classification
updated 7 months ago by
Ram
39k • written 9.5 years ago by
Nicholas Spies
★ 1.2k
114
votes
25
replies
65k
views
14 follow
Is My Bam File Sorted ?
bam
next-gen
sequencing
sort
updated 3 months ago by
Miguel
▴ 10 • written 12.3 years ago by
Pierre Lindenbaum
154k
45
votes
14
replies
65k
views
10 follow
Tutorial:
Using R in Conda
R
updated 11 weeks ago by
Ram
39k • written 2.9 years ago by
rohitsatyam102
▴ 690
35
votes
9
replies
65k
views
8 follow
Minor allele frequency calculation
SNP
next-gen
updated 12 months ago by
Ram
39k • written 8.6 years ago by
Tark
▴ 50
20
votes
15
replies
64k
views
12 follow
Python Script To Translate Rna Sequences To Protein Sequences
python
biopython
translation
updated 9.7 years ago by
viv_bio
▴ 50 • written 12.6 years ago by
Studentguy
▴ 70
51
votes
15
replies
64k
views
15 follow
Any Recommendations For Software For Drawing Plasmid Maps?
visualization
software
updated 13 months ago by
Ram
39k • written 12.0 years ago by
Michael Petronio
▴ 70
50
votes
15
replies
63k
views
12 follow
Sequence Number Count In Fastq.Gz File
counts
sequence
fastq
updated 3 months ago by
Ram
39k • written 11.9 years ago by
Bioscientist
★ 1.7k
53
votes
4
replies
63k
views
What is difference between soft-clipped and hard-clipped in SAM specification?
sequence
next-gen-sequencing
updated 7 months ago by
Ram
39k • written 8.5 years ago by
mangfu100
▴ 800
40
votes
7
replies
63k
views
Clustering Data (Rna-Seq) Using R To Produce A Heatmap
r
rna
heatmap
clustering
gene
updated 11 months ago by
Ram
39k • written 11.6 years ago by
Kanne
▴ 450
26
votes
10
replies
63k
views
9 follow
Tutorial:
Installing an updated R version (>=4.0) using conda
software-error
R
updated 11 weeks ago by
Ram
39k • written 2.2 years ago by
Nitin Narwade
★ 1.3k
25
votes
5
replies
61k
views
7 follow
Rpkm Calculation For Genes
rpkm
rna-seq
updated 10.6 years ago by
JC
13k • written 10.6 years ago by
siddharth.sethi5
▴ 270
8
votes
6
replies
61k
views
How To Calculate Coverage
fastq
coverage
updated 9.4 years ago by
r.follador
▴ 90 • written 9.4 years ago by
HG
★ 1.2k
361
votes
57
replies
61k
views
43 follow
Forum:
Recommend Your Favorite Bioinformatics Books
books
education
updated 8 months ago by
Kevin Blighe
85k • written 13.2 years ago by
Istvan Albert
98k
11
votes
9
replies
60k
views
7 follow
combining fasta files
windows
fasta
notepad
updated 6 months ago by
Devarakonda
• 0 • written 8.4 years ago by
vigneshprbh37
▴ 30
110,335 results • Page
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Recent Votes
Comment: Regular Expression for conversion
Answer: RNAseq Data and Pipeline
Comment: Salmon Index
Comment: Salmon Index
Resequencing data of pangenome
A: PolyPhen Humdiv vs HumVar discrepancies
A: How To Get Annotation For Bed File From Another Bed File
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Comment: How to make a dotplot for bulk RNA average expression ?
by
bhumm
▴ 40
I am not entirely clear on what you are trying to accomplish, but based on @Trivas answer, I would first transform the data relative to ave…
Answer: What is the best way to clean bulk RNA-seq data?
by
swbarnes2
13k
There really is no "best". Just find something reasonable, and document what you chose.
Comment: How to deal with duplicated gene IDs in TCGA RNA expression data?
by
Vincent Laufer
★ 2.8k
ok interesting. Do all, most, some, or a few of the genes have repeated lines. Is there a handy link to the exact file you are looking at?
Answer: Make a BedGraph file
by
bhumm
▴ 40
Bedtools window piped into genomecov? https://bedtools.readthedocs.io/en/latest/content/tools/window.html https://bedtools.readthedocs.io…
Answer: RNAseq Data and Pipeline
by
ATpoint
72k
STAR counts are the raw counts, that is appropriate for DESeq2.
Comment: RNAseq Data and Pipeline
by
swbarnes2
13k
STAR has a GeneCounts output that looks as you describe; three columns, depending on whether one thinks the prep is unstranded, or stranded…
Comment: RNAseq Data and Pipeline
by
Ram
39k
There is at least one more step that you haven't shared, isn't there? This line alone does not get you the data. You don't need to give us …
Comment: RNAseq Data and Pipeline
by
turcoa1
• 0
my entire code is not done. All I am trying to understand is if the STAR - counts downloaded from this query have raw reads. There are head…
Comment: RNAseq Data and Pipeline
by
Ram
39k
Please provide your entire code so those of us wishing to do a quick test can download and check for ourselves. Also, please use the format…
Comment: RNAseq Data and Pipeline
by
turcoa1
• 0
```r query <- GDCquery( project = "TCGA-THCA", data.category = "Transcriptome Profiling", data.type = "Gene Expression Quantificatio…
Comment: NCBI Common Taxonomy Tree
by
GenoMax
129k
It may be best to email NCBI help desk with these questions. Post their response here once you hear back from them.
Comment: RNAseq Data and Pipeline
by
Ram
39k
What data are you downloading? Can you show us your GDCquery commands please?
Comment: Salmon Index
by
ashleymb1116
• 0
I am working on cow, Bos Taurus. The Arabidopsis code was an example code that was on Salmon's github and an example for indexing they gave…
Comment: Salmon Index
by
GenoMax
129k
> I am using the Bos Taurus files Which organism are you actually working on? You have mentioned three so far, arabidopsis, human and now …
Comment: Salmon Index
by
ashleymb1116
• 0
Thank you, yes I am using the newer version of Ensembl. Above with Arabidopsis is the example Salmon gave, I am using the Bos Taurus files.…
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