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121,765 results • Page
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0
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248
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bamCoverage with DNAse-seq shift
bigwig
dnase-seq
visualization
deeptools
14 hours ago by
JMR
▴ 160
0
votes
0
replies
414
views
Job:
Assistant, Associate, or Full Project Scientist in Cell & Developmental Biology
Biology
UCSD
Cell
18 hours ago by
UCSDBioSci
▴ 30
0
votes
1
reply
722
views
RNA-Seq - Strand-specific coverage for a paired library
Bowtie2
bam
RNA-Seq
alignment
updated 19 hours ago by
cmdcolin
★ 4.3k • written 22 hours ago by
npb27
• 0
4
votes
3
replies
883
views
Tool:
Resource: A searchable library of 100,000+ scientific figures for visualization inspiration
visualization
11 hours ago by
mert
▴ 20
845
votes
170
replies
187k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
3.6k • written 8.7 years ago by
Istvan Albert
103k
0
votes
1
reply
743
views
vg gbwt missing sample and haplotype metadata when created using minigraph-cactus with vg construct
construct
gbwt
vg
minigraph-cactus
updated 21 hours ago by
Jouni Sirén
▴ 750 • written 22 hours ago by
cassiwatt
▴ 10
0
votes
1
reply
713
views
trouble: getting gene names from chromosome location using intersect function in bedtools
intersect
chromosome
location
geneIDs
bedtools
updated 19 hours ago by
GenoMax
153k • written 22 hours ago by
Sky
▴ 10
1
vote
0
replies
545
views
Job:
Resident Academic Full-Time Post in Bioinformatics (University of Malta)
bioinformatics
tenure
academia
23 hours ago by
jp
▴ 130
0
votes
0
replies
537
views
GO Terms significance Scoring
go
semantic
annotation
23 hours ago by
Marek
• 0
0
votes
0
replies
575
views
GSEA vs GO enrichment
go
kegg
gsea
1 day ago by
HarperReed
• 0
2
votes
6
replies
1.8k
views
Human WGS analysis system requirements
Human
NGS
WGS
analysis
Server
updated 1 day ago by
GenoMax
153k • written 1 day ago by
genomics_buddy
• 0
0
votes
0
replies
1.2k
views
Help with Error in cellchat
cellchat
updated 1 day ago by
GenoMax
153k • written 1 day ago by
Chris
▴ 360
0
votes
0
replies
1.4k
views
questioning about PCA graph in RNASeq analysis
bioinformatics-RNAseq
1 day ago by
Alaa
• 0
0
votes
6
replies
3.1k
views
Fungal Genome Assembly: In a messed up situation
Illumina
Pacbio
Nanopore
assembly
HiFi
1 day ago by
SomeOne
▴ 240
0
votes
1
reply
2.0k
views
statistical analysis
scRNA
statistics
updated 1 day ago by
ATpoint
89k • written 1 day ago by
archanaverma433
▴ 10
0
votes
0
replies
1.4k
views
News:
online course: Introduction to Python Programming for Biologists
Python
BioPython
Coding
1 day ago by
Physalia-courses
★ 2.6k
1
vote
1
reply
2.0k
views
Can I use raw read counts from the GTEx data for cross-tissue comparison?
rnaseq
gtex
statistics
normalization
updated 2 days ago by
GenoMax
153k • written 2 days ago by
mukherjeeliana24
• 0
4
votes
2
replies
2.7k
views
Forum:
FDR correction can sometimes lie to you!
correction
statistics
FDR
testing
multiple
updated 2 days ago by
LChart
5.0k • written 3 days ago by
Chakri
▴ 40
4
votes
9
replies
4.4k
views
Running metaSPAdes with 71M paired-end reads: RAM and swap memory concerns
metaspades
assembly
metagenomics
2 days ago by
sumah.1si21bt017
▴ 30
0
votes
1
reply
2.7k
views
Mageck RuntimeWarning overflow encountered in multiply
crispr
python
mageckmle
mageck
linux
updated 2 days ago by
ATpoint
89k • written 2 days ago by
liz.b
• 0
1
vote
5
replies
3.2k
views
Phage whole genome sequence submission by Bankit
phage
submission
updated 2 days ago by
acvill
▴ 350 • written 3 days ago by
Parto
• 0
0
votes
1
reply
2.3k
views
Any alternative way to do ASAP (Assemble Species by Automatic Partitioning)?
delimitation
Species
test
updated 3 days ago by
Mark
★ 1.7k • written 3 days ago by
Chatchapon
• 0
0
votes
1
reply
2.8k
views
How to determine the relative abundance
Abundance
Relative
Genomics
Metagenomics
updated 3 days ago by
sumah.1si21bt017
▴ 30 • written 3.4 years ago by
serene.s
• 0
0
votes
1
reply
2.4k
views
How to correctly report exon number for CRISPR-Cas9 knockout when genes have multiple isoforms?
numbering
exon
CRISPR-Cas9
updated 3 days ago by
ATpoint
89k • written 3 days ago by
jakob.vanek
• 0
0
votes
0
replies
2.1k
views
Read- and Assembly-based taxonomic classification of mixed (Prokaryote + Eukaryote) metagenomes
Shotgun
Taxonomy
metagenomic
3 days ago by
sumah.1si21bt017
▴ 30
0
votes
2
replies
2.4k
views
Refcall in VCF
pass
vcf
phasing
pipeline
Refcall
updated 3 days ago by
prasundutta87
▴ 720 • written 10 days ago by
Mahdi&Christ
▴ 20
1
vote
3
replies
316
views
Huge discrepancy between Pipseeker & DRAGEN for Pipseq data
analysis
sequencing
scRNA
seurat
updated 3 days ago by
GenoMax
153k • written 4 days ago by
Arindam
▴ 10
5
votes
7
replies
2.6k
views
how to download gene annotation for NCBI GENE ID
R
entrez
NCBI
updated 4 days ago by
josev.die
▴ 70 • written 7 days ago by
Bioinfonext
▴ 470
0
votes
0
replies
2.1k
views
News:
Genome Assembly using long-reads PacBio data
HIC
LongReads
GenomeAssembly
PacBio
4 days ago by
Physalia-courses
★ 2.6k
0
votes
0
replies
2.1k
views
Problem creating STRUCTURE inputs with PLINK
vcf
population
plink
structure
genetics
4 days ago by
Begonia_pavonina
▴ 210
2
votes
2
replies
2.5k
views
Taxonomic classification of shotgun metagenomic data of rhizospheric soil (bacteria + fungi)
Shotgun
classification
Taxonomic
metagenomics
5 days ago by
sumah.1si21bt017
▴ 30
0
votes
2
replies
2.2k
views
I encountered an error while using R Diffbind for data analysis
Diffbind
R
4 days ago by
Pallondyle
• 0
0
votes
1
reply
2.4k
views
News:
Single-Cell RNA-Seq Data Analysis: A Practical Introduction (October 6-8 in Berlin, Germany)
scRNAseq
transcriptomics
singleCell
5 days ago by
ecSeq Bioinformatics
▴ 20
0
votes
1
reply
2.2k
views
Single cell multiomics data i.e, sc(RNA+ATAC) seq data tar file
single
omics
cell
multi
updated 5 days ago by
ATpoint
89k • written 6 days ago by
Rupanjali
• 0
5
votes
4
replies
2.4k
views
Rust-HTSlib Script Only Outputs BAM Header, Records Are Missing
Rust
BAM
rust_htslib
5 days ago by
plusone
▴ 50
4
votes
2
replies
2.3k
views
galaxy and RNA seq
seq
RNA
5 days ago by
nmozdoori
▴ 20
0
votes
0
replies
2.1k
views
DIABLO Multi-omics Integration
How
results
interpret
DIABLO
to
updated 5 days ago by
GenoMax
153k • written 6 days ago by
Haya
• 0
0
votes
1
reply
3.2k
views
Which metric is best to represent cell-level counts/features in Seurat: raw/log-normalized (nCount_RNA/nFeature_RNA) or SCTransform (nCount_SCT/nFeat…
R
visualization
updated 7 days ago by
ATpoint
89k • written 14 days ago by
solo.albif
• 0
0
votes
2
replies
2.7k
views
Job:
Help with bioinformatics/genetics content of my CV
terminology
CV
updated 7 days ago by
Anchal
• 0 • written 15 days ago by
mairena.hirschberg
• 0
0
votes
3
replies
2.4k
views
Where can I get Human microbiome dataset
microbiome
human
data
updated 4 days ago by
Arup Ghosh
3.4k • written 7 days ago by
Emily
▴ 70
0
votes
11
replies
2.6k
views
chimeraTE GTF format error
chimeraTE
updated 3 days ago by
lieven.sterck
15k • written 7 days ago by
frarodmar17
• 0
6
votes
6
replies
2.6k
views
Estimating copy number of heterologous genes from WGS data in Yarrowia lipolytica
wgs
CNVs
copy-number
updated 6 days ago by
colindaven
7.8k • written 9 days ago by
Giorgia
▴ 10
0
votes
0
replies
2.2k
views
News:
online course (4th edition): Structural Variant Detection & Comparison
LongReads
Nanopore
PacBio
StructuralVariations
7 days ago by
Physalia-courses
★ 2.6k
1
vote
0
replies
2.2k
views
News:
Cellosaurus release 53 + new SPARQL/RDF functionalities
database
biocuration
line
knowledgebase
cell
8 days ago by
Amos Bairoch
▴ 140
0
votes
2
replies
2.4k
views
Relative viral load estimation from NGS data – is it possible?
virology
updated 3 days ago by
Mark
★ 1.7k • written 8 days ago by
omicon
▴ 40
0
votes
0
replies
2.2k
views
Best Practice for Group Composition Plots in Phyloseq
phyloseq
microbiome
relative-abundance
8 days ago by
bioinfo
▴ 60
1
vote
1
reply
2.3k
views
primer
primer
updated 8 days ago by
GenoMax
153k • written 8 days ago by
elahe
• 0
0
votes
0
replies
2.2k
views
Help installing and running PITA & PicTar for miRNA target prediction
microRNA
PITA
PicTar
updated 8 days ago by
Ram
45k • written 8 days ago by
Andie
• 0
1
vote
3
replies
2.4k
views
Splitting VCF using phase information
phasing
bcftools
vcf
updated 4 days ago by
cmdcolin
★ 4.3k • written 8 days ago by
Kyra
• 0
0
votes
0
replies
2.2k
views
Generating VCF file from Illumina MEGA array
strand
VCF
SNP
illumina
microarray
8 days ago by
112233
• 0
121,765 results • Page
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Resource: A searchable library of 100,000+ scientific figures for visualization inspiration
Comment: Resource: A searchable library of 100,000+ scientific figures for visualization
Comment: Resource: A searchable library of 100,000+ scientific figures for visualization
Resource: A searchable library of 100,000+ scientific figures for visualization inspiration
Resident Academic Full-Time Post in Bioinformatics (University of Malta)
Answer: Comparison between scRNA and bulk RNA
Answer: Human WGS analysis system requirements
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Comment: Resource: A searchable library of 100,000+ scientific figures for visualization
by
mert
▴ 20
I trained a model using yolo.
Comment: Low percentage of 'Fraction Antibody Reads Usable' in Feature Barcode Cell Range
by
octopuslegs11
▴ 10
I don't want to piggyback on this but did you figure out what the issue was? I'm also experiencing this issue!
Comment: Resource: A searchable library of 100,000+ scientific figures for visualization
by
jared.andrews07
★ 19k
How did you manage the plot extraction? This is indeed very useful.
Answer: RNA-Seq - Strand-specific coverage for a paired library
by
cmdcolin
★ 4.3k
I think you got the idea with the "4 separate files" and combining them first two and second two. A good point of reference is the IGV "fir…
Comment: Resource: A searchable library of 100,000+ scientific figures for visualization
by
Jeremy Leipzig
23k
ok this is actually very cool. good job
Answer: trouble: getting gene names from chromosome location using intersect function in
by
GenoMax
153k
If you just need gene names added to the BED file of interest then this will require conversion of the GTF to BED to extract things of inte…
Answer: vg gbwt missing sample and haplotype metadata when created using minigraph-cactu
by
Jouni Sirén
▴ 750
If you want to build a graph from a reference and a VCF file, we recommend using `vg autoindex`. The best practices depend on the data you …
Comment: Human WGS analysis system requirements
by
GenoMax
153k
> It is a for only sample. You also need to tell us how much data you have. If you had a billion (or two) reads then it may be a different…
Comment: Fungal Genome Assembly: In a messed up situation
by
SomeOne
▴ 240
Hi, thank you for the suggestions. Technically, with Nanopore I got reads between 8000-9000 bp long (median length of reads in sample) but…
Comment: Human WGS analysis system requirements
by
colindaven
7.8k
Theres heaps of IO so SSD speed is very important. You can monitor IO with a tool like bottom https://github.com/ClementTsang/bottom
Comment: Human WGS analysis system requirements
by
genomics_buddy
• 0
Thank you for the reply. I do have an other HDD of 4 TB. So, storage i can manage. My primary concern is RAM and threads, only due to heavy…
Comment: Human WGS analysis system requirements
by
genomics_buddy
• 0
Thanks a lot for your suggestions. Yes, It is a for only sample. I do have a extra 4 TB HDD. So, storage i can manage i think. My only prim…
Answer: Human WGS analysis system requirements
by
colindaven
7.8k
For one sample at a time that should be enough, although the storage looks very slim. I'd get an extra much bigger SSD and external backups…
Answer: Human WGS analysis system requirements
by
ATpoint
89k
If with "WGS" you mean mapping and variant calling then yes. Threads and RAM are sufficient (for a single sample). Question is how many sam…
Comment: statistical analysis
by
ATpoint
89k
Why not sticking to established analysis? Each meta-program is essentially a geneset. I would test for differential expression via limma (o…
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