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121,445 results • Page
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GrCh37 or GrCh38? That is the question.
GrCh37
sequence
human
genome
GrCh38
5 minutes ago by
Anjan
▴ 840
0
votes
3
replies
55
views
Error Correction with Tadpole and BBMerge
BBmerge
tadpole
error
correction
updated just now by
Mensur Dlakic
★ 29k • written 3 hours ago by
Jon
• 0
0
votes
2
replies
281
views
Scvi - Integration
integration
singlecell
scvi
written 6 days ago by
t.foskolos
• 0
840
votes
170
replies
180k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 8 weeks ago by
Biostar
3.6k • written 8.6 years ago by
Istvan Albert
102k
3
votes
2
replies
125
views
Bootstrapping in RNA-seq
bootstrapping
RNAseq
updated 6 hours ago by
swbarnes2
15k • written 12 hours ago by
littlebioinformatician
• 0
0
votes
1
reply
137
views
Computing ka/ks ratio for individual genes.
ks
ka
evolution
selection
updated 7 hours ago by
shelkmike
★ 1.6k • written 2 days ago by
Shakunthala Natarajan
• 0
2
votes
4
replies
149
views
Log2FC track Visualization in IGV
visualization
IGV
RNASeq
6 hours ago by
SomeOne
▴ 200
0
votes
0
replies
76
views
Job:
2-3 Permanent Positions as Bioinformatician/Computational Biologist at Swedish national research infrastructures
Sweden
SciLifeLab
Genomics
Uppsala
Proteomics
10 hours ago by
Matthias Zepper
5.1k
2
votes
2
replies
92
views
Obtaining a BED/GTF from Ensembl
rna-seq
ensembl
bcftools
rna
10 hours ago by
joe_genome
▴ 60
0
votes
0
replies
57
views
News:
New Bioconductor and Physalia Collaboration!
Bioconductor
DataAnalysis
Genomics
13 hours ago by
Physalia-courses
★ 2.6k
1
vote
1
reply
152
views
Read1 and read2 asymmetry in stranded, paired-end rnaseq data aligned by STAR
stranded
STAR
RNAseq
antisense
sense
updated 16 hours ago by
rfran010
★ 1.6k • written 1 day ago by
yampolsk
▴ 10
0
votes
1
reply
144
views
Does the order of SplitNCigarReads and MarkDuplicates affect RNA-seq variant calling results?
variantcalling.
rnaseq
gatk
updated 16 hours ago by
rfran010
★ 1.6k • written 2 days ago by
iamsmor
• 0
0
votes
0
replies
55
views
Should I standardize age and age2 as covariates in GWAS models?
GWAS
covariates
17 hours ago by
yu
• 0
3
votes
5
replies
381
views
Venn Diagram
VennDiagram
updated 19 hours ago by
jianhua.mert
• 0 • written 3 days ago by
Nasim Gandomdoust
• 0
0
votes
1
reply
97
views
PhageTerm error
phage
genome
phageterm
assamble
updated 10 hours ago by
GenoMax
152k • written 20 hours ago by
NIkita
• 0
0
votes
1
reply
96
views
WGCNA Workflow from GEO FASTQ Files – Expression Type, Sample Inclusion, and Preprocessing
fastq
wgcna
rna
updated 20 hours ago by
GenoMax
152k • written 22 hours ago by
tutorwhizzz
• 0
0
votes
0
replies
69
views
small RNA-Seq pipeline Standstill
NGS
miRNA
23 hours ago by
anthony.santana.703.j
▴ 30
2
votes
2
replies
152
views
comparison interpretation
model
design
contrast
matrix
updated 16 hours ago by
rfran010
★ 1.6k • written 1 day ago by
jkim
▴ 190
0
votes
0
replies
69
views
piRNAs Analysis Pipelines
piRNAs
smallRNA
1 day ago by
GA
• 0
1
vote
1
reply
106
views
Beagle PATH (partially) recognised on Ubuntu 22.04
Ubuntu
Beagle
Beast
MCMC
updated 1 day ago by
GenoMax
152k • written 1 day ago by
Luca Arbore
▴ 10
3
votes
2
replies
148
views
custom BED to UCSC genome browser
browser
ucsc
ucsc-genome-browser
genome
methylation
bed
updated 1 day ago by
Maximilian Haeussler
★ 1.7k • written 1 day ago by
npont
▴ 10
0
votes
0
replies
96
views
CNV Computation with HoneyBadger
CNV
HoneyBADGER
updated 1 day ago by
GenoMax
152k • written 1 day ago by
mete.han.celebi
• 0
0
votes
0
replies
89
views
How to predict programatically TFBS and calculate PWM Score as JASPAR
PWMScan
TFBS
JASPAR
1 day ago by
t.kajiyama
• 0
0
votes
0
replies
139
views
News:
Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introduction (September 10-12, 2025 in Berlin, Germany)
Workshop
RNA-Seq
Illumina
VariantCalling
DNA-Seq
1 day ago by
ecSeq Bioinformatics
▴ 20
0
votes
14
replies
550
views
Host Removal Issues Human/Dog with samples containing Eukaryotic species
Eukarotic
Mapping
Host
updated 1 day ago by
Mensur Dlakic
★ 29k • written 2 days ago by
Jon
• 0
1
vote
1
reply
187
views
Pharokka Error
error
annotating
viral
pharokka
genome
updated 2 days ago by
Mensur Dlakic
★ 29k • written 2 days ago by
NIkita
• 0
0
votes
0
replies
119
views
Combining Metatranscriptomics (Mtx) and Metagenomics (Mgx)
metagenomics
reference-based
Metatranscriptomics
alignment
updated 2 days ago by
GenoMax
152k • written 2 days ago by
rk009
• 0
1
vote
1
reply
177
views
how to make a circular plot of variants
circular
plot
updated 2 days ago by
Pierre Lindenbaum
166k • written 2 days ago by
analyst
▴ 70
2
votes
6
replies
323
views
GSEA analysis
RNA-seq
RNA
analysis
updated 2 days ago by
GenoMax
152k • written 2 days ago by
Ahmed
• 0
0
votes
0
replies
117
views
how to make go term plot and kegg pathway plot using InterProScan output
interproscan
2 days ago by
analyst
▴ 70
1
vote
3
replies
470
views
Why little difference in RNAseq alignment when flipping forward and reverse strand with paired end reads
mRNA
rna-seq
bowtie
alignment
updated 2 days ago by
swbarnes2
15k • written 3 days ago by
JourneyToAbyss
▴ 250
0
votes
2
replies
238
views
DESeq2 design for differential expression with 2 timepoints and 2 controls
Transcriptomic
Feeding
DESeq2
RNAseq
updated 1 day ago by
jkim
▴ 190 • written 3 days ago by
Cristina
• 0
0
votes
1
reply
204
views
Questions about sample_accession_id and file_accession_id from EGA files
EGA
open
WGS
data
updated 3 days ago by
GenoMax
152k • written 3 days ago by
askif4
▴ 20
4
votes
3
replies
332
views
Example of ClinVar pathogenic variant where the reference allele is pathogenic?
snp
variant
pathogenic
clinvar
updated 3 days ago by
GenoMax
152k • written 4 days ago by
a615ebfb
▴ 60
0
votes
1
reply
218
views
Functional Analysis Using EMU Output – Has Anyone Tried This?
emu
updated 4 days ago by
andres.firrincieli
3.9k • written 4 days ago by
henrique.mouradias
• 0
1
vote
2
replies
244
views
featureCounts -t option not working in v2.0.8?
subread
featureCounts
RNAseq
updated 2 hours ago by
GenoMax
152k • written 4 days ago by
atan
• 0
0
votes
1
reply
226
views
mm10 blacklist regions detailed information
sequencing
DNA
blacklist
updated 4 days ago by
GenoMax
152k • written 4 days ago by
annaA
▴ 10
0
votes
2
replies
259
views
identify unmapped regions
human
illumina
libraries
1 day ago by
María José
▴ 10
0
votes
1
reply
353
views
martCheck - Error - You must provide a valid Mart object - for running HoneyBADGER for CNV computation
CNV
biomaRt
HoneyBADGER
4 days ago by
mete.han.celebi
• 0
0
votes
1
reply
270
views
How to Specify Power Parameter for Adjacency in modulePreservation() (WGCNA)
module
analysis
WGCNA
preservation
updated 4 days ago by
andres.firrincieli
3.9k • written 4 days ago by
Berkan
• 0
0
votes
1
reply
241
views
filters used in mutation discovery from RNAseq data
filter
RNAseq
mutation
updated 4 days ago by
GenoMax
152k • written 4 days ago by
htchd
▴ 20
2
votes
4
replies
405
views
Trouble Using GEO Files with Seurat
seurat
GEO
scRNA
updated 14 hours ago by
antonioggsousa
3.4k • written 5 days ago by
Jamie
• 0
0
votes
0
replies
222
views
Knowledge Graph for AI
KnowledgeGraph
biotech
AI
SPOKE
Bioinformatician
5 days ago by
Cornelio
• 0
2
votes
2
replies
951
views
How to convert from .spec to .bam?
bam
file-formatting
spec
updated 5 days ago by
Daniel Lai
▴ 10 • written 4.8 years ago by
gmora
▴ 30
2
votes
1
reply
275
views
Insert size is zero
rna-seq
fastp
insert
size
updated 5 days ago by
GenoMax
152k • written 5 days ago by
SpliceAndScript
• 0
0
votes
3
replies
383
views
Should matched samples (not paired) be included in the DESeq2 design model?
edgeR
limma
DESeq2
updated 4 days ago by
i.sudbery
21k • written 5 days ago by
marieke
• 0
0
votes
4
replies
388
views
Trajectory Analysis scRNA-seq with one cell type and two developmental stages
cellrank
palantir
pseudotime-trajectory-analysis
scrna-seq
trajectory-analysis
4 days ago by
npont
▴ 10
0
votes
1
reply
615
views
How to run trajectory analysis with only one cell type using palantir?
palantir
scanpy
updated 5 days ago by
npont
▴ 10 • written 11 months ago by
bioinfo
▴ 160
0
votes
1
reply
262
views
should I remove the transcripts from annotate peak region in ATAC-seq
ATAC-seq
updated 4 days ago by
rfran010
★ 1.6k • written 5 days ago by
QX
▴ 70
3
votes
2
replies
359
views
DESeq2 LRT test with multifactorial design
test
design
multifactorial
LRT
5 days ago by
lessismore
★ 1.4k
121,445 results • Page
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Recent Votes
Understanding MarkDuplicates and featureCounts results
METAL-Random
Answer: custom BED to UCSC genome browser
Cluster sub-set analysis using Seurat
Subclustering work-flow suggestions in Seurat
Technical replicates in RNAseq
Comment: Log2FC track Visualization in IGV
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Mensur Dlakic
★ 29k
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Recent Replies
Comment: Error Correction with Tadpole and BBMerge
by
Mensur Dlakic
★ 29k
Not exactly an answer to your question, but it still may be useful. If you are going to assemble with SPAdes in the end, it does error cor…
Comment: Error Correction with Tadpole and BBMerge
by
Jon
• 0
So of all of the sets that I'm working with, they are all 150x2 reads. I have 2 sets that were done on Novaseq 6000, I believe these are…
Answer: Error Correction with Tadpole and BBMerge
by
GenoMax
152k
> Can I use BBduk for example against the reference to pull reads out that match specific species, and error correct them separately Filte…
Comment: featureCounts -t option not working in v2.0.8?
by
atan
• 0
Unfortunately, I'm working on a managed cluster, so I can't test with the older version easily. But it's the same GTF as before. In both ca…
Comment: Log2FC track Visualization in IGV
by
SomeOne
▴ 200
Noted, THank you for hilighting this
Answer: Bootstrapping in RNA-seq
by
swbarnes2
15k
You could try to figure out what PC2 represents. If it's something meaningful, like tissue contamination. You could also include PC2 a…
Comment: Log2FC track Visualization in IGV
by
rfran010
★ 1.6k
Yes, that is a valid and common way to generate the track. There's also bamCompare to make the comparison in one step. Note, with RNA-seq…
Answer: Computing ka/ks ratio for individual genes.
by
shelkmike
★ 1.6k
It's better to use PAML to calculate Ka/Ks. With PAML, you can use the so-called "branch model", which allows you to calculate Ka/Ks for ea…
Comment: Log2FC track Visualization in IGV
by
SomeOne
▴ 200
the bars are going Up and Down the axis, and labeled as Log2 kmt6/wt, my guess is that its based on the foldchange ? With chatGpt i was ab…
Comment: Log2FC track Visualization in IGV
by
GenoMax
152k
From the figure legend: > Changes in transcription for low nitrogen conditions are shown in > purple as log2 value of the **RPKM** ratio …
Comment: PhageTerm error
by
GenoMax
152k
Is `python v.3.x` default python on your machine? It looks like someone else had reported an identical error last year ( https://sourcefor…
Comment: Obtaining a BED/GTF from Ensembl
by
joe_genome
▴ 60
I'll take a look, looking for Alu repeats specifically.
Comment: Obtaining a BED/GTF from Ensembl
by
GenoMax
152k
There is a prior solution available, if you are able to make it work (requires Perl) --> https://www.biostars.org/p/9549836/#9549985
Answer: Bootstrapping in RNA-seq
by
ATpoint
88k
Your PC1 explains 75% of variance, driven by the group separation, I would expect lots of DEGs. Rather than home-cooked approaches like boo…
Comment: Trouble Using GEO Files with Seurat
by
antonioggsousa
3.4k
I do not know if they are exactly the same, but I think they are equivalent from its description ([link](https://anndata.readthedocs.io/en/…
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