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I'm thinking ideally there would be UMIs. Since it's targeting an isolated, repetitive region, it's hard to determine if all the duplicates…
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Thanks for responding.
I am going to try it again soon.
I am using a Mac but I suppose I can still use those path descriptions in the…
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I don't think batch correction is necessary here, especially since you just want to compare gene expression within a condition.
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Please ask `Galaxy` related questions on their help forum: https://help.galaxyproject.org/
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