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116,664 results • Page
1 of 2334
Sort: Rank
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Votes
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0
votes
1
reply
158
views
Extract protein sequence
fasta
alignment
blast
updated 1 hour ago by
Ram
43k • written 1 day ago by
anna
▴ 20
0
votes
0
replies
21
views
Post-imputation plot
michigan-imputation-server
quality-control
updated 2 hours ago by
Ram
43k • written 2 hours ago by
kl
▴ 10
0
votes
3
replies
152
views
Is it possible to bulk download files from GEO repository?
RNA-seq
Galaxy
Tanscriptomics
updated 2 hours ago by
noodle
▴ 570 • written 13 hours ago by
WUSCHEL
▴ 750
0
votes
4
replies
119
views
Programmatically retrieving positions of protein active site residues
Uniprot
PDB
Proteins
updated 8 minutes ago by
Wayne
★ 2.0k • written 5 hours ago by
Mariana
▴ 10
0
votes
1
reply
57
views
metagenomic virome quality assessment
viromics
metagenomics
updated 2 hours ago by
Ram
43k • written 5 hours ago by
Petchimuthu
• 0
795
votes
167
replies
142k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
3
votes
7
replies
99
views
FastQC Quality per tile and per sequence behaving strange after using Cutadapt
fastqc
cutadapt
illumina
tile
paired-end
1 hour ago by
salias
• 0
0
votes
0
replies
25
views
Using limma in methylation EM-seq?
EM-seq
methylation
3 hours ago by
Lluís R.
★ 1.2k
4
votes
7
replies
461
views
Very low RNA splicing rate for pulmonary AT2 cells
RNA-velocity
scVelo
scRNA-seq
splicing
2 hours ago by
e.r.zakiev
▴ 200
0
votes
0
replies
34
views
Synteny analysis
bacterial
synteny
genome
updated 3 hours ago by
GenoMax
141k • written 3 hours ago by
Namil
• 0
0
votes
2
replies
67
views
Genbank File Format
gbkformat
updated 2 hours ago by
Mensur Dlakic
★ 27k • written 3 hours ago by
alenew.am
• 0
0
votes
5
replies
256
views
How should I make kallisto indexes?
kallisto
updated 1 hour ago by
dsull
★ 5.9k • written 14 days ago by
bioinfo
▴ 150
0
votes
0
replies
53
views
Assist me in determining whether the analysis process using the limma package has been executed correctly
limma
ArrayExpress
DifferentialExpression
GEOquery
4 hours ago by
SSSJec
• 0
0
votes
3
replies
85
views
Software to separate reads from different individuals
software
development
nanopore
updated just now by
GenoMax
141k • written 4 hours ago by
njornet
▴ 20
0
votes
0
replies
31
views
Seeking Guidance on Identifying Mutations in DARs from ATAC Data in Cancer Genomes
Genomics
Bioinformatics
ATACseq
CancerResearch
5 hours ago by
David
• 0
3
votes
5
replies
164
views
High Malat-1 expression in single cell data
cell
analysis
single
updated 5 hours ago by
dsull
★ 5.9k • written 8 hours ago by
Kazo
▴ 10
0
votes
2
replies
85
views
How to update R on ubuntu
installation
update
R
apt-get
4 hours ago by
Bosberg
▴ 50
3
votes
0
replies
53
views
Herald:
The Biostar Herald for Monday, April 29, 2024
herald
6 hours ago by
Biostar
2.7k
0
votes
1
reply
79
views
Two references 1. genome 2. plasmid for bowtie2
WGS
BacterialGenomics
Alignment
updated 7 hours ago by
GenoMax
141k • written 11 hours ago by
Ruqaiya
• 0
0
votes
1
reply
231
views
Using Delly/Pindel/breakdancer for Identifying Transgene Insertion Sites in Mouse Genome
breakdancer
insertion
Pindel
variation
Delly
updated 7 hours ago by
trausch
★ 1.9k • written 16 days ago by
zec018
• 0
0
votes
0
replies
49
views
LEfSe
LEfSe
7 hours ago by
benkosta
• 0
0
votes
0
replies
39
views
How should I handle read counts derived from SGSeq when I want to build DEXSeqDataSet object
DEXSeq
DEXSeqDataSet
SGSeq
5 hours ago by
Sara
▴ 30
1
vote
4
replies
310
views
Limma Analysis Agilent Microarray Data (GPL1708)
Microarray
Limma
Agilent
5 hours ago by
hagl
▴ 10
0
votes
1
reply
81
views
RNAseq one control two conditions, shared and exclusive genes
conditions
RNAseq
multiple
Rstudio
updated 8 hours ago by
Mohamed Abderrahmane
▴ 20 • written 8 hours ago by
matteo.levorato
• 0
0
votes
2
replies
91
views
Longitudinal analysis of subpopulations: which approach is better?
differential-expression
DEG
model
8 hours ago by
Lluís R.
★ 1.2k
0
votes
1
reply
89
views
How to solve DESeq2 Error in checkFullRank(modelMatrix)?
RNA-Seq
DESeq2
updated 10 hours ago by
i.sudbery
19k • written 11 hours ago by
DOBI
• 0
0
votes
0
replies
54
views
Comparing peptide sequences with MS/MS peptide data using MaxQuant
Transcriptomics
Mass
Bioinformatics
spectrometry
Proteins
11 hours ago by
atharvakarkare14
▴ 10
5
votes
16
replies
547
views
How many reads for WGS Sequencing?
WGS
Bacterial-Genomics
11 hours ago by
Ruqaiya
• 0
0
votes
1
reply
87
views
What should I consider as FASTA for dataset?
PDB
FASTA
updated 7 hours ago by
GenoMax
141k • written 12 hours ago by
Nafi
• 0
34
votes
22
replies
1.5k
views
10 follow
NGS forensics: how to know if data is fabricated
fastq
STAR
NGS
Illumina
37 minutes ago by
noodle
▴ 570
0
votes
0
replies
47
views
Differential accessibility using DiffBinf
diffbind
13 hours ago by
Shloka
• 0
0
votes
0
replies
52
views
vg call vs vg surject
vg
variation
graphs
updated 7 hours ago by
GenoMax
141k • written 14 hours ago by
aliraza3119
• 0
0
votes
1
reply
80
views
Can I merge Hi-C fastq files from different lanes?
GenomeAssembly
BWA-MEM2
Hi-C
updated 6 hours ago by
GenoMax
141k • written 14 hours ago by
Winter
• 0
2
votes
7
replies
1.2k
views
6 follow
Differential Expression using Isoseq-supplemented reference transcriptome
RNA-Seq
Salmon
Isoseq
updated 18 hours ago by
Gordon Smyth
★ 7.1k • written 8 months ago by
Calum
▴ 10
0
votes
1
reply
161
views
Finding batch and outlayers
Pca
updated 10 hours ago by
christopher medway
▴ 450 • written 19 hours ago by
Tigran
• 0
2
votes
2
replies
149
views
PDB related issue
rcsb
pdb
updated 19 hours ago by
noodle
▴ 570 • written 22 hours ago by
Nafi
• 0
0
votes
0
replies
78
views
News:
Online course: Genome Annotation
Genome-Assembly
Genome-Annotation
9 hours ago by
carlopecoraro2
★ 2.5k
0
votes
7
replies
294
views
What does it mean single base resolution in sequencing?
SNP
sequencing
updated 5 hours ago by
dsull
★ 5.9k • written 1 day ago by
jinyu
▴ 10
0
votes
9
replies
2.5k
views
6 follow
Cannot process all the reads in a fast5 file?
metagenome
base-calling
fastq
nanopore
updated 13 hours ago by
Ram
43k • written 8 months ago by
Gio
• 0
2
votes
5
replies
403
views
Fetch table from clinvar database according to a list of rsid
python
clinvar
perl
1 day ago by
ashaneev07
▴ 20
0
votes
1
reply
126
views
Downloading full alignments from Pfam
pfam
updated 1 day ago by
GenoMax
141k • written 1 day ago by
bef1
• 0
0
votes
1
reply
718
views
Can I run cellassign on samples independently if there is batch effect present?
RNA-Seq
R
cellassign
batch-effect
updated 1 day ago by
Francesco
▴ 10 • written 3.3 years ago by
gt
▴ 30
2
votes
2
replies
247
views
ChIP-seq datasets: input samples omitted?
ChIP-seq
Normalization
NGS
updated 10 hours ago by
i.sudbery
19k • written 3 days ago by
vanbelj
▴ 40
0
votes
0
replies
90
views
adjusting for confounders in LMER in R
confounders
LMER
R
updated 3 hours ago by
dariober
14k • written 1 day ago by
rene.j.erhardt
▴ 20
1
vote
3
replies
244
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 1 day ago by
ATpoint
82k • written 1 day ago by
Francesco
▴ 10
0
votes
0
replies
114
views
STAR total splices (in Log.final) vs collapsed splice junctions (in SJ.out.tab)
STAR
1 day ago by
tnminh89
▴ 10
0
votes
0
replies
120
views
Filter low express genes in microarray data
microarray
1 day ago by
Chris
▴ 260
0
votes
1
reply
306
views
absolute path for symbolic links in Snakefile
Snakemake
updated 2 days ago by
Jesse
▴ 740 • written 10 days ago by
yifangt86
▴ 60
0
votes
2
replies
319
views
Hide positions in alignment with 99% "–" characters to ignore single sequence insertions?
alignment
gaps
updated 2 days ago by
Jesse
▴ 740 • written 4 days ago by
Broccoli
• 0
2
votes
3
replies
315
views
Source other conda environments in a nextflow pipeline when nextflow itself is in a conda environment?
hpc
conda
nextflow
updated 2 days ago by
Arup Ghosh
3.2k • written 3 days ago by
chaco001
▴ 40
116,664 results • Page
1 of 2334
Recent Votes
Comment: How to calculate identity percentage between proteins contained in a FASTA file?
Comment: How to calculate identity percentage between proteins contained in a FASTA file?
Answer: How to use limma to find differentially expressed genes in response to a continu
Answer: How to use limma to find differentially expressed genes in response to a continu
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
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Recent Replies
Comment: Programmatically retrieving positions of protein active site residues
by
Wayne
★ 2.0k
Wow, I was shocked at this AI answer that looks to be a good start for using UniProt. Sharing it in case it is useful. (It will work in ses…
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 570
> Can you clarify what you mean by "100% of reads pass cutadapt, even > though 70% of reads contain adapters and get trimmed. The header o…
Comment: Software to separate reads from different individuals
by
GenoMax
141k
If you have independent information available (e.g. genotype data for both individuals) then you *may* be able to assign reads based on the…
Answer: Extract protein sequence
by
Matthias Zepper
4.5k
You didn't mention what kind of organism you are working with and what kind of protein you are looking for? If it happens to be a microbia…
Comment: How to use limma to find differentially expressed genes in response to a continu
by
pairedttest
▴ 10
Thank you Gordon for your expertise. One question that I have now that I have a list of DE genes is how to interpret logFC of this continuo…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
• 0
Yes I plan to use QIIME2, but I'm Cutadapting outside QIIME so its easier to MultiQC without importing/unzipping QZAs (and QIIME2 importing…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
GenoMax
141k
> 3' adapters were removed aprox. 90 times each one, so there sohuld not be read-through at all here Then you have data that has poor Q sc…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
• 0
Following your advice, I checked the primer and adapters sequences and edited the question accordingly
Comment: How should I make kallisto indexes?
by
dsull
★ 5.9k
We can't phase them out per se, because ``kallisto index`` is still the engine responsible for creating the index under the hood (and there…
Comment: When to use .vcf or .gvcf files from GATK HaplotypeCaller?
by
Medhat
9.7k
"./." in your gVCF files typically indicates a lack of information or uncertainty about the genotype at that particular position. This co…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
• 0
Fungal ITS2 region (length is really variable so read-through can be an issue)
Answer: Is it possible to bulk download files from GEO repository?
by
noodle
▴ 570
Also try the NCBI AWS bucket transfer tool. It's extremely convenient, however it's not instantaneous and you need to have a (paid) AWS acc…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
GenoMax
141k
Check a random sampling of reads to make sure the adapters are being removed. You may also want to blast a few reads to ensure that there i…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
• 0
Many thanks for your comment. If read-through is happening, shouldn't Cutadapt being taking care of that there? I provided to the Cutadapt …
Comment: How should I make kallisto indexes?
by
bioinfo
▴ 150
Thank you. I will read the documentation you attached. Are you going to slowly phase out the not kb ref commands? Also, does the t2g.txt fi…
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