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121,373 results • Page
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2
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6
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Different comparisons in RNASeq Analysis
DEGs
RNAseq
updated 2 hours ago by
mbyvcm
▴ 460 • written 7 hours ago by
SomeOne
▴ 200
0
votes
1
reply
60
views
Querying on non-canonical bacterial IDs
microbes
protein
accession
updated 2 hours ago by
Mensur Dlakic
★ 29k • written 3 hours ago by
benjamin.kellman
• 0
0
votes
2
replies
106
views
Distribution of assayed SNPs per sample
distribution
PLINK
missingness
updated 2 hours ago by
mbyvcm
▴ 460 • written 9 hours ago by
am29
▴ 60
0
votes
0
replies
64
views
News:
Learn Python for Biological Data Analysis – Online Course this September!
NGS
Python
Programming
10 hours ago by
Physalia-courses
★ 2.6k
839
votes
170
replies
179k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 weeks ago by
Biostar
3.6k • written 8.5 years ago by
Istvan Albert
102k
3
votes
0
replies
98
views
Tutorial:
How to calculate partial correlation controlling cancer types
CRISPR
biology
correlation
computational
bioinformatics
20 hours ago by
Ming Tommy Tang
★ 4.6k
0
votes
1
reply
121
views
SAIGE-GENE error
R
SAIGE
updated 1 day ago by
GenoMax
151k • written 1 day ago by
smyiz
▴ 30
2
votes
1
reply
295
views
IGV track display problem: interval rectangle from BED file appears above all tracks in white, overlapping each other
IGV
bed
1 day ago by
mcsimenc
▴ 30
2
votes
1
reply
153
views
Y-axis break in Volcano Plot
Volcano_Plot
Enhanced_Volcano
updated 1 day ago by
GenoMax
151k • written 1 day ago by
rajdeepboral00
▴ 70
1
vote
0
replies
107
views
How to rank a gene list using correlation coefficient and p-values for GSEA/clusterProfiler?
ClusterProfiler
GSEA
correlation
rank
genelist
1 day ago by
c-wes
▴ 10
2
votes
4
replies
287
views
Nanopore data analysis
nanopore
updated 1 day ago by
colindaven
7.6k • written 1 day ago by
sarahmanderni
▴ 130
6
votes
2
replies
1.4k
views
What is the difference between norm --multiallelics -any versus --atomize?
bcftools
updated 1 day ago by
ashotmarg2004
▴ 130 • written 21 months ago by
a615ebfb
▴ 50
0
votes
1
reply
144
views
checking the sequences that share origin
sequencing
updated 1 day ago by
GenoMax
151k • written 1 day ago by
QX
▴ 70
0
votes
0
replies
107
views
News:
Assembling Genomes with Oxford Nanopore: A Hands-On Guide to Long-Read Sequencing
Nanopore
GenomeAssembly
longreads
1 day ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
211
views
Bambu on illumina short reads sequencing
Bambu
updated 1 day ago by
Rob
7.1k • written 1 day ago by
Simone
• 0
0
votes
0
replies
119
views
Isoquant Major Exon Elongation
isoquant
nanopore
exon
2 days ago by
aico
• 0
0
votes
0
replies
125
views
bcftools run-roh error
roh
bcftools
updated 2 days ago by
GenoMax
151k • written 2 days ago by
Pratiksha
• 0
0
votes
0
replies
136
views
News:
Workshop: Virtual Single-Cell Transcriptomics, June 24–26
Transcriptomics
workshop
UConn
2 days ago by
zsc25001
▴ 10
1
vote
6
replies
306
views
How can I find KO IDs for ORF sequences from kofam_scan ?
biology
Computational
Bioinformatics
2 days ago by
Nikesh
• 0
0
votes
0
replies
141
views
VEP all genes are skipped due to invalid types
variants
GTF
VEP
annotation
2 days ago by
Titus
▴ 910
4
votes
3
replies
291
views
Isoform analysis after quantification with Salmon/Star
STAR
Salmon
Isoform
analysis
1 day ago by
rajdeepboral00
▴ 70
0
votes
0
replies
127
views
Confidence in CIBERSORT (LM22) Deconvolution Output for Rare Cell Types — Best Practices?
LM22
DECONVOLUTION
CIBERSORT
2 days ago by
Pumla
• 0
1
vote
7
replies
375
views
Significant reads loss after fastp
fastp
loss
reads
updated 1 day ago by
GenoMax
151k • written 2 days ago by
SpliceAndScript
• 0
1
vote
1
reply
195
views
Shinyapps Server Down?
Shinyapps
Posit
updated 2 days ago by
GenoMax
151k • written 2 days ago by
Arup Ghosh
3.3k
0
votes
2
replies
253
views
Data of microarray
Microarray
1 day ago by
Peter
• 0
0
votes
0
replies
166
views
How to run GSVA on scRNA-Seq analysis on multiple clusters and groups to compare per tumor sample?
GSVA
scRNA-Seq
2 days ago by
Minh-Anh
▴ 10
2
votes
4
replies
332
views
Best Tool for Fast RNA-Seq Transcriptome Alignment to Extract Unmapped Reads
mapping
rna-seq
2 days ago by
aeb
▴ 20
0
votes
2
replies
247
views
Time scaled tree with IQTree giving off looking branch length
bacteria
phylogeny
time
evolution
scaled
2 days ago by
SushiRoll
▴ 140
1
vote
2
replies
267
views
Signature Matrix Modifications/Subclustering
signature
matrix
scRNA-seq
seurat
cibersortx
3 days ago by
MitchT
▴ 10
4
votes
2
replies
256
views
Picard CollectRawWgsMetrics runs much slower on non-Apple chip machine
picard
updated 3 days ago by
Michael
55k • written 3 days ago by
adixon3
• 0
0
votes
0
replies
178
views
Reporting Imputed SNPs in GWAS
impute
GWAS
updated 3 days ago by
Ram
45k • written 3 days ago by
Aastha
• 0
0
votes
0
replies
156
views
Does Beagle use the reference genome for both phasing AND imputing genotypes?
beagle
genotypes
phasing
imputation
3 days ago by
giulia.trauzzi
▴ 30
0
votes
0
replies
156
views
In GERPplusplus, odd gerpelem outputs
GERP
evolutionary
gerpcol
substitution
gerpelem
rates
rejected
3 days ago by
Begonia_pavonina
▴ 210
6
votes
2
replies
260
views
required annotations for VQSR
VQSR
vcf
annotation
3 days ago by
Mahdi&Christ
▴ 20
4
votes
4
replies
374
views
Identify transgene insert loci/copy number in nanopore De-novo assembly
ont
nanopore
transgene
sequencing
wgs
3 days ago by
ctmfarland
▴ 20
9
votes
9
replies
557
views
Heatmap for visualizing the differentially expressed genes in RNA-seq data
R
DEseq2
RNA-seq
differentially-expressed
heatmap
3 days ago by
KABILAN
▴ 130
0
votes
1
reply
242
views
single-cell differential expression of unbalanced cell number
Seurat
scRNA-seq
updated 3 days ago by
ATpoint
88k • written 3 days ago by
Yingying
▴ 10
0
votes
1
reply
761
views
Differential Expression tests for cell-type clusters from a single-cell RNAseq dataset with no biological replicates
cell-type
DEG
DE
single-cell
updated 3 days ago by
Yingying
▴ 10 • written 5 months ago by
aa23871x
• 0
2
votes
10
replies
536
views
Annotation Issue using miRBASE gff
NGS
miRNA
updated 2 days ago by
GenoMax
151k • written 3 days ago by
anthony.santana.703.j
▴ 30
0
votes
0
replies
195
views
News:
Evo 2 webinar - 1 day left to register
AI.LLM.Biology.Genomics
4 days ago by
Claire Watson
▴ 60
1
vote
6
replies
382
views
Error Scaffolding with RagTag
scaffold
RagTag
updated 3 days ago by
Michael
55k • written 4 days ago by
Bipin
▴ 10
4
votes
0
replies
221
views
Tool:
biothings-mcp - MCP server to query genes, variants, chemicals, taxons
API
biothings
genes
AI
MCP
4 days ago by
Anton
▴ 100
2
votes
0
replies
196
views
Tool:
synergy-age-mcp - a database of synergistic and antagonistic genetic interactions in longevity
combination
longevity
MCP
ageing
4 days ago by
Anton
▴ 100
2
votes
0
replies
212
views
Tool:
opengenes-mcp - MCP server on the database for longevity genes
genes
databases
bioinformatics
longevity
MCP
4 days ago by
Anton
▴ 100
2
votes
0
replies
217
views
Tool:
gget-mcp - a bioinformatics toolkit for genomics queries and analysis (based on gget lbrary)
bioinformatics
libraries
MCP
AI
4 days ago by
Anton
▴ 100
0
votes
2
replies
257
views
Best pairwise alignment for insertion and deletion-containing sequences
sequence
python
biopython
pairwise
alignment
4 days ago by
rbpdee
▴ 50
2
votes
7
replies
486
views
How to deal with intermediate files
procedures
data
operations
updated 4 days ago by
GenoMax
151k • written 4 days ago by
ramiro.barrantes
▴ 60
1
vote
1
reply
234
views
Quality control metrics in mmCIF
PDB
protein
mmCIF
updated 4 days ago by
dthorbur
★ 3.0k • written 4 days ago by
Adrian
• 0
0
votes
1
reply
231
views
phage sequence no alignment to genome
prophage
bwa
phage
genome
alignment
updated 4 days ago by
GenoMax
151k • written 4 days ago by
Luwell
• 0
0
votes
1
reply
241
views
Mitochondrial Annotation Tool
Illumina
Bovine
Mitochondria
updated 4 days ago by
patrickdm
▴ 260 • written 4 days ago by
adarsh
▴ 60
121,373 results • Page
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Recent Replies
Comment: Distribution of assayed SNPs per sample
by
mbyvcm
▴ 460
> According to my logic, one could do this by filtering out SNPs with missing genotype calls (./.), however when I did this, I ended up hav…
Answer: Different comparisons in RNASeq Analysis
by
mbyvcm
▴ 460
For analysis 3, you could think about something like: (Plant Mut vs. Plant WT) vs (Flask Mut vs. Flask WT) In doing so you are internally…
Answer: Querying on non-canonical bacterial IDs
by
Mensur Dlakic
★ 29k
I don't think there are any other records, including what you call canonical, for this protein. Maybe this will help you, using [**Entrez …
Comment: Different comparisons in RNASeq Analysis
by
jkim
▴ 190
See [the post](https://www.biostars.org/p/403053/#473727).
Comment: Different comparisons in RNASeq Analysis
by
SomeOne
▴ 200
Hi. Thank you for replying. If i trim the PE data to SE and at 75bp length, and perform the analysis. Wouldn’t that effect the final co…
Comment: Different comparisons in RNASeq Analysis
by
SomeOne
▴ 200
Hi! Thank you for your response. Can you share some information on how to remove this batch effect ?
Answer: Different comparisons in RNASeq Analysis
by
swbarnes2
15k
> or the Different sequence-type (paired vs single), difference in > read-length (150bp-x2 vs 75bp-x1) will have any technical or batch …
Comment: Different comparisons in RNASeq Analysis
by
jkim
▴ 190
For analyses 3 and 4, you will need to account for batch effects, which I expect to be substantial given the differences between datasets—e…
Comment: Distribution of assayed SNPs per sample
by
1769mkc
★ 1.3k
if you can share some part of the data that would be helpful what you are trying and what the final output expected , that helps for people…
Comment: Trouble building combined pangenome graph with circularized genomes + embedded O
by
Jordan M Eizenga
▴ 740
That error is caused by a simple failure to open that file (which is created in the `vg prune`). Usually, that results from a typo in the f…
Comment: SAIGE-GENE error
by
GenoMax
151k
Segfaults are related to memory, which is what part of the message is telling you. If you are running this job under a job scheduler make s…
Answer: IGV track display problem: interval rectangle from BED file appears above all tr
by
mcsimenc
▴ 30
The problem was the filename suffix, it's meaningful in how IGV treats the file. It is .pk here, but changed to .bed and it works.
Comment: Y-axis break in Volcano Plot
by
GenoMax
151k
> Any help is appreciated You should consider upvoting and validating (accepting, green check mark) comments/answers you have received on …
Comment: Isoform analysis after quantification with Salmon/Star
by
rajdeepboral00
▴ 70
Thank you for your detailed reply
Answer: Nanopore data analysis
by
colindaven
7.6k
- Merge bams - yes. (why do you have so many ?). Merging fastqs before creating bams is easier (cat *.fastq.gz ) than bam merging. - 150…
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