Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Post does not exist.
Limit : all time
all time
today
this week
this month
this year
121,535 results • Page
1 of 2431
Sort: Rank
Rank
Views
Votes
Replies
2
votes
13
replies
473
views
Which Reference Hg38 or T2T?? Both??
Host
Removal
updated 35 minutes ago by
GenoMax
152k • written 1 day ago by
Jon
• 0
0
votes
2
replies
57
views
Can I transduce HEK293T with Cas9 lentivirus followed by transfection with sgRNAs?
lentivirus
Transduction
CRISPR
transfection
offtopic
updated 1 hour ago by
jared.andrews07
★ 19k • written 3 hours ago by
louisflower1999
• 0
0
votes
0
replies
33
views
Preranked GSEA permutation
GSEA
4 hours ago by
as823jk
• 0
1
vote
2
replies
125
views
Mitochondrial annotation tool for algae
barcoding
annotation
updated 10 hours ago by
Juke34
9.2k • written 12 hours ago by
firefox91
• 0
0
votes
1
reply
104
views
Identifying gene outliers in a genome using composition analysis.
RSCU
composition
GC
outliers
updated 10 hours ago by
GenoMax
152k • written 13 hours ago by
Shakunthala Natarajan
• 0
843
votes
170
replies
182k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 10 weeks ago by
Biostar
3.6k • written 8.6 years ago by
Istvan Albert
102k
1
vote
3
replies
150
views
Mapping to a template sequece
sequencing
updated 9 hours ago by
GenoMax
152k • written 13 hours ago by
QX
▴ 70
0
votes
2
replies
205
views
quality control for 16s metagenome reads
quality
metagneome
control
16s
fastp
updated 11 hours ago by
GenoMax
152k • written 2 days ago by
m90
▴ 30
0
votes
2
replies
192
views
Loss of strandedness in the Illumina Strandedness Total RNA protocol
Illumina
RNA-seq
reads
stranded
updated 16 hours ago by
ATpoint
88k • written 21 hours ago by
Anjan
▴ 840
1
vote
3
replies
890
views
Problem with Mirdeep2 and Randfold output
mirdeep2
Randfold
updated 11 hours ago by
GenoMax
152k • written 14 months ago by
otieno43
▴ 40
0
votes
0
replies
136
views
Help using ShinyCell2 and Seurat v5 object.
shinycell
seurat
shiny
shinycell2
scRNA
1 day ago by
Jamie
• 0
0
votes
0
replies
91
views
News:
Machine Learning for Multi-Omics Integration at the University of Barcelona– Dec 2025
Machine-Learning
Sulti-Omics
Depp-Learning
Data-Integration
BigData
1 day ago by
Physalia-courses
★ 2.6k
0
votes
0
replies
125
views
Compare alternative splicing patterns between human and mouse?
orthologs
rna-seq
splicing
alternative
1 day ago by
aeb
▴ 20
2
votes
4
replies
229
views
Trouble getting representatives from clustered_nr database
database
blast
updated 6 hours ago by
GenoMax
152k • written 1 day ago by
Adolfo
• 0
0
votes
0
replies
107
views
FindTransferAnchors (reference prediction) vs Manual Annotations
Seurat
1 day ago by
reddyornah
• 0
3
votes
4
replies
253
views
NCBI Datasets CLI Question
ncbi-datasets
2 days ago by
Bjorn
• 0
0
votes
0
replies
128
views
Incomplete assembly in HPRC dataset
pangenome
HPRC
reference
2 days ago by
ohell
• 0
3
votes
3
replies
254
views
Joint Calling for Large Germline WGS Cohort
NGS
RAM
cohort
Genomics
WGS
updated 2 days ago by
Jeremy Leipzig
23k • written 2 days ago by
j.k3096
▴ 20
0
votes
4
replies
260
views
how shoud the phred score be intepreted?
sequencing
updated 2 days ago by
GenoMax
152k • written 2 days ago by
QX
▴ 70
0
votes
3
replies
368
views
Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be external SNPs?
WASP
updated 1 day ago by
i.sudbery
21k • written 5 days ago by
jonas.andersson
▴ 40
0
votes
0
replies
126
views
Identifying RBP motifs overlapping exons
motifs
bedtools
RBP
2 days ago by
RK
• 0
0
votes
0
replies
130
views
WGCNA analysis for Proteomics data
Proteomics
WGCNA
2 days ago by
Sumit Paliwal
▴ 40
3
votes
8
replies
6.6k
views
7 follow
Cellranger count pipestance failed: The read lengths are incompatible with all the chemistries
10x
cellranger
single-cell
updated 2 days ago by
zhang
• 0 • written 3.0 years ago by
firestar
★ 1.7k
0
votes
1
reply
600
views
News:
Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introduction (September 10-12, 2025 in Berlin, Germany)
Workshop
RNA-Seq
Illumina
VariantCalling
DNA-Seq
2 days ago by
ecSeq Bioinformatics
▴ 20
0
votes
0
replies
134
views
How to merge multiple pangenome graph files (generated by MC and PGGB)?
vg
pangenome
Minigraph-Cactus
PGGB
2 days ago by
Yao
▴ 30
1
vote
0
replies
201
views
Can iHS and XP-EHH be applied to targeted sequencing data?
sequencing
targeted
ngs
ihs
4 days ago by
slzr_
▴ 10
1
vote
0
replies
211
views
Identify SNP, Indel variants from a List of FASTA sequence
SNP
4 days ago by
Trinh
▴ 10
2
votes
3
replies
385
views
PacBio amplicon reads partially aligned using minimap2 – library or analysis issue?
PacBio
minimap2
alignment
variants
pbmm2
2 days ago by
louisflower1999
• 0
0
votes
0
replies
707
views
Job:
Biomedical Data Scientist / Systems Biologist / Omics Data Analyst / Bioinformatician / Computational Biologist (Tulane University, New Orleans, LA)
TUPulm
5 days ago by
yzhou40
• 0
0
votes
0
replies
260
views
Batch effect in codominant microsatellite data – how to correct
population-genetics
batch-effect
R
microsatellite
genotyping
5 days ago by
shervin
• 0
2
votes
3
replies
393
views
UCSC genome browser negative strand positions
Genomic
Data
Browser
UCSC
Visualization
Genome
updated 5 days ago by
Maximilian Haeussler
★ 1.8k • written 6 days ago by
ijarne
▴ 20
0
votes
1
reply
278
views
Error when computing bedtools bamtobed -bedpe
human
bedtools
libraries
illumina
bamtobed
updated 5 days ago by
ATpoint
88k • written 5 days ago by
María José
▴ 10
0
votes
9
replies
802
views
Ligand-Receptor analysis using LIANA - question about specificity
scRNA-seq
communication
cell-cell
updated 5 days ago by
Muhammad
▴ 10 • written 9 days ago by
abedkurdi10
▴ 190
2
votes
4
replies
1.2k
views
PRS calculation from two sample genotype results
prs
calculation
updated 5 days ago by
Muhammad
▴ 10 • written 6 months ago by
Arun
• 0
1
vote
16
replies
6.0k
views
PRS in UK Biobank - no covariate file and no phenotype file
UK
Biobank
PRS
3.8 years ago by
Jalil Sharif
▴ 80
40
votes
14
replies
2.7k
views
13 follow
Forum:
How do you estimate time to complete a project or task?
career
ngs
updated 5 days ago by
Анна
• 0 • written 5 months ago by
sviatoslav.kendall
▴ 990
0
votes
0
replies
215
views
News:
Introduction to Deep Learning for Biologists - online course, 3–7 November
MachineLearning
Python
deepLearning
ConvolutionalNeuralNetwork
5 days ago by
Physalia-courses
★ 2.6k
1
vote
2
replies
844
views
Running trajectory and velocity analysis with multiple samples
seurat
velocity
loom
trajectory
written 15 months ago by
gogeni5529
▴ 70
4
votes
7
replies
630
views
Weird p-value distribution on edgeR results
edgeR
pvalue-distribution
gene-expression
5 hours ago by
Guillermo
▴ 10
0
votes
1
reply
284
views
NMR modelling using xplor-NIH
peptide
xplor-NIH
NMR
5 days ago by
npk107
• 0
1
vote
5
replies
438
views
PCA interpretation
chip
seq
PCA
updated 15 hours ago by
ATpoint
88k • written 6 days ago by
Irving
• 0
0
votes
0
replies
244
views
News:
course: Reproducibility in Bioinformatics - Only 2 seats remain!
Singularity
Git
Reproducibility
Docker
Snakemake
6 days ago by
Physalia-courses
★ 2.6k
0
votes
0
replies
234
views
Annotate phylogenetic trees with bars or strips
otu
phylogenetics
R
taxonomy
metabarcoding
6 days ago by
Luca Arbore
▴ 10
2
votes
2
replies
443
views
Identifying Long Reads Spanning Chromosomal Breakpoints
ONT
samtools
Whole-Genome
updated 6 days ago by
trausch
★ 2.0k • written 8 days ago by
Noah
▴ 10
0
votes
2
replies
372
views
PAML output
PAML
Codeml
Selection
updated 2 days ago by
cfos4698
★ 1.1k • written 7 days ago by
Morjina
• 0
1
vote
0
replies
293
views
how to produce your own Nx plot
R
contiguity
plot
Nx
7 days ago by
Matteo Ungaro
▴ 130
1
vote
2
replies
425
views
Interpreting inconsistency between BLAST hits and phylogenetic clustering i
tree
phylogenetic
blast
6 days ago by
triplee0305
▴ 20
0
votes
0
replies
261
views
News:
Online course - Environmental Metagenomics - 13-17 October
Illumina
MAGs
Nanopore
Metagenomics
7 days ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
392
views
Deg Difference in Deseq2
Deg
updated 7 days ago by
swbarnes2
15k • written 7 days ago by
j.k.owaresat
• 0
0
votes
0
replies
308
views
No gene_trans_map file in CD-HIT-EST output
cd-hit-est
Trinity
RNAseq
Trinotate
updated 7 days ago by
GenoMax
152k • written 7 days ago by
ofarinas621
• 0
121,535 results • Page
1 of 2431
Recent Votes
Answer: Which Reference Hg38 or T2T?? Both??
Answer: Weird p-value distribution on edgeR results
Answer: Weird p-value distribution on edgeR results
Answer: Which Reference Hg38 or T2T?? Both??
Comment: Mapping to a template sequece
Comment: Mitochondrial annotation tool for algae
Answer: Picking optimal resolution for single cell in seurat pipeline
Recent Locations •
All
United States,
5 minutes ago
United States,
18 minutes ago
United States,
24 minutes ago
United States,
34 minutes ago
United States,
51 minutes ago
United Kingdom,
57 minutes ago
Memphis, TN,
59 minutes ago
Recent Awards •
All
Popular Question
to
Eduardo
▴ 20
Popular Question
to
firefox91
• 0
Popular Question
to
Anjan
▴ 840
Popular Question
to
asalimih
▴ 60
Teacher
to
Kevin Blighe
89k
Popular Question
to
Zev.Kronenberg
12k
Popular Question
to
zoepklein
• 0
Recent Replies
Comment: Which Reference Hg38 or T2T?? Both??
by
GenoMax
152k
BBMap will do fasta input. With ~4-5 kb contigs you should switch to using `minimap2` ([**LINK**][1]) for alignments. You can simply use th…
Comment: Which Reference Hg38 or T2T?? Both??
by
Jon
• 0
I have read that Eukaryotes most difficult to classify, I can see now the issue, they have high similarity to human dna. Amoebas, such as…
Answer: Can I transduce HEK293T with Cas9 lentivirus followed by transfection with sgRNA
by
jared.andrews07
★ 19k
You might be interested in the [MuLE system](https://www.addgene.org/kits/mule-system/), which I've had some success with in the past. It's…
Comment: Can I transduce HEK293T with Cas9 lentivirus followed by transfection with sgRNA
by
GenoMax
152k
While someone may answer, this post does not fit the theme of this forum. Please ask this question on biology stackexchange or a relevant r…
Comment: 2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
by
Simon
• 0
Hi is this position still available? I couldn’t not find it on the site.
Comment: Weird p-value distribution on edgeR results
by
Guillermo
▴ 10
Thanks! Limma-Voom lmFit approach fixed the problem
Comment: Trouble getting representatives from clustered_nr database
by
GenoMax
152k
> get-cluster-representatives-for-taxid.sh that comes with clustered_nr and got a list of accessions of viridiplantae Was that list huge? …
Comment: Which Reference Hg38 or T2T?? Both??
by
GenoMax
152k
This is an interesting study. Assuming OP has done everything right there is a reasonable hypothesis here. OP needs to prove it, with an in…
Comment: Which Reference Hg38 or T2T?? Both??
by
cmdcolin
★ 4.2k
Thanks for the link. This is good background on what the OP is 'truly' asking here. The finding of N fowleri would be probably very serious…
Comment: Which Reference Hg38 or T2T?? Both??
by
GenoMax
152k
> So along that line, I was looking at the Accession number for the human identified reads, these are all VERY old, and mostly prior to hg3…
Comment: Which Reference Hg38 or T2T?? Both??
by
GenoMax
152k
While this tool looks interesting, things are not as straight forward as they sound based on the main question in this thread (please refer…
Answer: Which Reference Hg38 or T2T?? Both??
by
cmdcolin
★ 4.2k
There are existing specialized tools for host read removal such as https://github.com/bede/hostile (publication https://academic.oup.com/…
Comment: Mapping to a template sequece
by
GenoMax
152k
Not clear what you are trying to do here. Are you looking for quantify the "diverse" tags in your data or is it something else?
Comment: Mapping to a template sequece
by
QX
▴ 70
the template is look like this: -----[fix base ~100bp]-------[20bp varying/diverse]------[fix-remain]---[20bp varying/diverse]-------[fix b…
Comment: Mitochondrial annotation tool for algae
by
Juke34
9.2k
The last version of it is here => [https://juke34.github.io/awesome-genome-annotation/][1] [1]: https://juke34.github.io/awesome-genome…
Traffic: 2732 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6