Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Post does not exist.
Limit : all time
all time
today
this week
this month
this year
117,212 results • Page
1 of 2345
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
1
view
Mailing list for academic programs and positions
masters
phd
just now by
Eren
• 0
0
votes
1
reply
42
views
Batch correction prior to running DESEQ2
R
RNAseq
DESEQ2
DGE
transcriptomics
updated 2 hours ago by
ATpoint
83k • written 3 hours ago by
jayju
• 0
2
votes
2
replies
108
views
News:
Online - Autumn School in Bioinformatics
Singularity
NGS
Phylogenomics
RNA-seq
Docker
updated 4 hours ago by
Mensur Dlakic
★ 27k • written 10 hours ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
42
views
DMRs on subset of genes methylation array data
DMR
DMRs
methylation
EPIC
5 hours ago by
kaaz
• 0
0
votes
1
reply
68
views
Add Subscripts to labels in Seurat DimPlot
DimPlot
ggplot2
Seurat
RStudio
updated 5 hours ago by
bk11
★ 2.5k • written 6 hours ago by
Saran
▴ 50
4
votes
10
replies
315
views
RNA-seq differentially expressed genes (DEGs) with low fold change values
ComBat-seq
RNA-seq
DESeq2
DEGs
1 hour ago by
singlecell_bio
• 0
801
votes
167
replies
144k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 4 months ago by
Biostar
2.8k • written 7.5 years ago by
Istvan Albert
100k
0
votes
3
replies
107
views
find DEG of monocle 3 object using tradeSeq
tradeSeq
updated 3 hours ago by
Ram
44k • written 7 hours ago by
synat.keam
▴ 100
0
votes
1
reply
69
views
Are there different ways of calculating log2FC?/How do I manually calculate gene log2FCs for continuous variables?
scRNA-seq
updated 3 hours ago by
ATpoint
83k • written 7 hours ago by
j.parker1993
• 0
0
votes
2
replies
101
views
QC filtering for 10x Single Cell Gene Expression Flex Fixed RNA Profiling
10x
Seurat
scRNA-Seq
updated 3 hours ago by
ATpoint
83k • written 9 hours ago by
yura.grabovska
▴ 90
0
votes
2
replies
127
views
RNA seq
Deseq2
updated 6 hours ago by
Ram
44k • written 10 hours ago by
Sudip
• 0
0
votes
1
reply
108
views
InferCNV results interpretation
CNV
inferCNV
scRNA-seq
updated 9 hours ago by
jared.andrews07
★ 17k • written 1 day ago by
Yingying
• 0
1
vote
2
replies
130
views
Combining microarray, RNA Seq, whole & exome sequencing
Microarray
RNA
updated 12 hours ago by
i.sudbery
19k • written 15 hours ago by
Bine
▴ 60
8
votes
5
replies
392
views
PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while adj p < 0.05 does not.
RNA-seq
padj
p-value
updated 6 hours ago by
swbarnes2
14k • written 7 days ago by
May Ling
▴ 10
1
vote
1
reply
67
views
Alternative Database for Somatic Indel Realignment (COSMIC Alternative)
COSMIC
updated 12 hours ago by
DGTool
▴ 30 • written 12 hours ago by
ManuelDB
▴ 80
0
votes
2
replies
131
views
Minimap2 chimeric reads
minimap2
chimeric-reads
updated 9 hours ago by
cmdcolin
★ 3.8k • written 15 hours ago by
Fadwa
▴ 10
0
votes
1
reply
91
views
incomplete data after running CreateSeuratObject in Seurat for scRNAseq data
scRNAseq
updated 6 hours ago by
Ram
44k • written 13 hours ago by
Sara
▴ 260
0
votes
4
replies
145
views
small RNA-seq length distribution of 100nt?
small-rnaseq
fastp
mirnas
updated 12 hours ago by
GenoMax
142k • written 14 hours ago by
karlaarz
▴ 110
0
votes
1
reply
102
views
Exome sequencing dataset for SKAT
SKAT
VEP
exome-sequencing
updated 15 hours ago by
Pierre Lindenbaum
162k • written 21 hours ago by
aurina.arnatk
▴ 10
0
votes
0
replies
53
views
Estimate/calculate power for two-sample Mendelian randomization?
MR
15 hours ago by
huynguyenacademia
• 0
0
votes
0
replies
59
views
Blog:
Long-read single-cell Sequencing
RNASeq
Long-read
single-cell
scRNA-Seq
transcriptomics
17 hours ago by
Gabby
• 0
0
votes
1
reply
103
views
TSS sites annotation for hg38
tss
hg38
annotation
updated 11 hours ago by
GenoMax
142k • written 17 hours ago by
octpus616
▴ 100
0
votes
3
replies
147
views
Cut&Run replicates handling
peakcaller
replicates
SEACR
chipseq
updated 16 hours ago by
Asaf
10k • written 21 hours ago by
g.anande
• 0
0
votes
0
replies
70
views
kegg pathway analysis
_analysis
metabolic_pathway
Gibbs_energy_calculate
18 hours ago by
yuxiang
• 0
0
votes
1
reply
103
views
newbie question: why the length of adapter is not fixed?
Library
construction
Sequencing
updated 12 hours ago by
GenoMax
142k • written 21 hours ago by
Dora
▴ 10
1
vote
5
replies
188
views
CNVnator Installation
CNV
CNVnator
WGS
updated 8 hours ago by
Ram
44k • written 22 hours ago by
Wasim
• 0
0
votes
0
replies
79
views
deeptools Compute reference-point and scale-regions
deeptools
computeMatrix
plotprofile
1 day ago by
RD
▴ 10
1
vote
5
replies
253
views
Creating a Seurat object with Visium public available data
Spatial
Visium
10X
updated 1 day ago by
Ram
44k • written 1 day ago by
charms.charms
• 0
4
votes
2
replies
664
views
Using samtools with GCS (google cloud storage) on a docker container seems to give "Protocol Not Supported" error
samtools
gcs
updated 17 days ago by
aw7
▴ 330 • written 5 weeks ago by
abhishekghadge
▴ 30
1
vote
7
replies
322
views
Can I assemble chloroplast genome of plants using SRA data from NCBI and I can publish it ?
Chloroplast
genome
SRA
updated 1 day ago by
Istvan Albert
100k • written 1 day ago by
balajir24.smc
• 0
1
vote
1
reply
146
views
Herald:
The Biostar Herald for Wednesday, May 29, 2024
herald
updated 1 day ago by
LChart
3.9k • written 1 day ago by
Biostar
2.8k
0
votes
1
reply
110
views
enrichGO function produces NULL object
enrichGO
updated 1 day ago by
bk11
★ 2.5k • written 1 day ago by
rykers
• 0
1
vote
2
replies
149
views
Forum:
How Can BioJobs Lab Effectively Attract Top Talent and Employers in the Biotech Industry?
biotech
updated 1 day ago by
pairedttest
▴ 20 • written 1 day ago by
Darren
• 0
0
votes
1
reply
205
views
vg call vs vg surject
vg
variation
graphs
updated 1 day ago by
glenn.hickey
▴ 520 • written 4 weeks ago by
aliraza3119
• 0
2
votes
4
replies
2.1k
views
How do I create a gff(3) file from a genebank file?
gff
genebank
updated 1 day ago by
Istvan Albert
100k • written 3.2 years ago by
torkel.loman
▴ 10
0
votes
0
replies
91
views
visualization of centromere
visualization
centromere
human
1 day ago by
kidpopaye
• 0
1
vote
1
reply
180
views
Job:
Seeking remote internship in genomic analyses
NGS
genomics
variant-calling
1 day ago by
SlowSD
• 0
5
votes
9
replies
368
views
VG view and VG chunk failed when using SequenceTubeMap
vg
vgteam
1 day ago by
gforg34
▴ 20
4
votes
2
replies
138
views
cannot conda install macs3 on linux
atac
macs3
1 day ago by
QX
• 0
2
votes
5
replies
307
views
NCBI vs Ensembl - Ortholog genes Information
Genomics
Orthologs
NCBI
Ensembl
updated 1 day ago by
tdmurphy
▴ 200 • written 2 days ago by
José
▴ 10
0
votes
2
replies
129
views
Chromosomal distribution of the trancscripts count
chrosome_location
updated 1 day ago by
ATpoint
83k • written 1 day ago by
Varsha
• 0
0
votes
4
replies
156
views
How to Split FASTQ File by UMI Indices into Multiple Files
SeqKit
UMI
split
fastq
updated 1 day ago by
GenoMax
142k • written 1 day ago by
LDT
▴ 340
0
votes
1
reply
97
views
Alternatives RAST annotation
database
alternative
RAST
annotation
updated 1 day ago by
GenoMax
142k • written 1 day ago by
emma.vankerckhove
• 0
0
votes
0
replies
92
views
Job:
Postdoctoral Fellow in Machine Learning for Neuroimaging Genomics at University of Otago – Dunedin, New Zealand
human-genomics
machine-learning
neuroimaging
1 day ago by
narun.pat
• 0
7
votes
7
replies
293
views
Constructing single sequence genome assembly out of short reads
short
reads
assembly
5 hours ago by
analyst
▴ 50
5
votes
8
replies
404
views
How do i calculate the mean of triplicates in a data.frame based on pattern?
tidyverse
r
updated 1 day ago by
ATpoint
83k • written 3 days ago by
Assa Yeroslaviz
★ 1.9k
3
votes
3
replies
213
views
RNA-Seq analysis
rna-seq
updated 1 day ago by
carlopecoraro2
★ 2.5k • written 1 day ago by
zahrakhanyousufzai5
• 0
1
vote
5
replies
218
views
bcl-convert cannot create output directory structure at the path specified
illumina
reads
bcl-convert
index
demultiplexing
updated 1 day ago by
GenoMax
142k • written 1 day ago by
giulia.trauzzi
▴ 10
0
votes
5
replies
239
views
Filtering reads that map to both references
bwa
insertion
alignment
reference
updated 1 day ago by
Trivas
★ 1.8k • written 1 day ago by
michael.flower.14
▴ 180
0
votes
0
replies
89
views
Results of NOT FOUND encountered when running SCENIC
SCENIC
1 day ago by
smilewang997700
• 0
117,212 results • Page
1 of 2345
Recent Votes
Comment: RNA-seq pipeline for degraded RNA
Comment: RNA-seq pipeline for degraded RNA
RNA-seq pipeline for degraded RNA
Consultant to update a Snakemake workflow with the new Kubernetes executor plugin for Snakemake 8
Answer: RNA-seq differentially expressed genes (DEGs) with low fold change values
Answer: RNA-seq differentially expressed genes (DEGs) with low fold change values
Answer: RNA-seq differentially expressed genes (DEGs) with low fold change values
Recent Locations •
All
United States,
1 minute ago
United States,
3 minutes ago
Türkiye,
5 minutes ago
Brazil,
5 minutes ago
San Diego,
10 minutes ago
United States,
16 minutes ago
Canada,
20 minutes ago
Recent Awards •
All
Popular Question
to
giammafer
▴ 20
Popular Question
to
ManuelDB
▴ 80
Popular Question
to
eebloom
▴ 80
Commentator
to
karlaarz
▴ 110
Supporter
to
QX
• 0
Popular Question
to
egascon
• 0
Popular Question
to
Gabby
• 0
Recent Replies
Comment: RNA-seq differentially expressed genes (DEGs) with low fold change values
by
singlecell_bio
• 0
Libraries were not prepped at the same time. The batches were 4 months apart.
Comment: RNA-seq differentially expressed genes (DEGs) with low fold change values
by
swbarnes2
14k
You know that merely putting a library on the instrument at a different time doesn't cause a batch effect, right? If the libraries were pr…
Comment: RNA-seq differentially expressed genes (DEGs) with low fold change values
by
singlecell_bio
• 0
Thank you for the context! Do you recommend including the different tissue types and timepoints in the same analysis to yield better result…
Comment: RNA-seq differentially expressed genes (DEGs) with low fold change values
by
swbarnes2
14k
For context, when I have different related tissues in the same RNASeq experiment, the first PC usually represents about 90% of the variance…
Comment: RNA-seq differentially expressed genes (DEGs) with low fold change values
by
singlecell_bio
• 0
Thank you for this insight! This is a great point and I totally agree. If this is a common trend with cell line based RNA-seq experiments, …
Comment: RNA-seq differentially expressed genes (DEGs) with low fold change values
by
singlecell_bio
• 0
Thank you for the suggestion! I did initially try it without combat-seq by simply including batch in the design, but the results weren't co…
Comment: InferCNV results interpretation
by
Kay
• 0
Does inferCNV tell you the number of CNVs at a location and what is the resolution for detecting CNVs?
Comment: Batch correction prior to running DESEQ2
by
ATpoint
83k
Please post the colData (a meaningful subset of it), so one can have a look. Almost certainly there is a suboptimal encoding of your variab…
Comment: QC filtering for 10x Single Cell Gene Expression Flex Fixed RNA Profiling
by
ATpoint
83k
Flex definitely includes mitochondrial genes (https://www.10xgenomics.com/support/software/cell-ranger/downloads#probe-set-downloads). Is t…
Comment: Are there different ways of calculating log2FC?/How do I manually calculate gene
by
ATpoint
83k
I would not bother and export the logcounts, and test with limma which supports any design of arbitrary complexity. It will give (moderated…
Comment: find DEG of monocle 3 object using tradeSeq
by
Ram
44k
N I don't - it's not my area of expertise.
Comment: find DEG of monocle 3 object using tradeSeq
by
synat.keam
▴ 100
Thanks, Ram. Ill follow your instruction next time. Do you have any idea with my question?
Comment: Online - Autumn School in Bioinformatics
by
Mensur Dlakic
★ 27k
How come this is not called Autumn School in NGS? The title seems to be a bit misleading.
Comment: Online - Autumn School in Bioinformatics
by
Nat.Nataren
▴ 30
Will there be any recordings of this workshop or at least the material made available online after? Looks really great, but the time zone …
Comment: Constructing single sequence genome assembly out of short reads
by
analyst
▴ 50
Which tool or approach should I use to perform reference guided assembly?
Traffic: 1453 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6