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41 results • Page
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0
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1
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14
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Source other conda environments in a nextflow pipeline when nextflow itself is in a conda environment?
hpc
conda
nextflow
updated 11 minutes ago by
ATpoint
82k • written 16 minutes ago by
chaco001
▴ 40
0
votes
0
replies
10
views
Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
edgeR
RNA-Seq
comparisons
multiple
26 minutes ago by
Guille
• 0
0
votes
0
replies
28
views
ChIP-seq datasets: input samples omitted?
ChIP-seq
Normalization
NGS
updated 2 hours ago by
Ram
43k • written 2 hours ago by
vanbelj
▴ 40
0
votes
0
replies
35
views
Limma Analysis Agilent Microarray Data (GPL1708)
Microarray
Limma
Agilent
updated 1 hour ago by
GenoMax
141k • written 3 hours ago by
hagl
▴ 10
0
votes
4
replies
353
views
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 4 hours ago by
pinheirofabiano
▴ 10 • written 3 days ago by
qudrat.nii
▴ 10
0
votes
5
replies
262
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 2 hours ago by
Pierre Lindenbaum
161k • written 13 hours ago by
J
• 0
19
votes
11
replies
14k
views
10 follow
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated 3 hours ago by
cmdcolin
★ 3.8k • written 10.8 years ago by
J.F.Jiang
▴ 920
2
votes
2
replies
169
views
what is the purpose of indexing the reference genome (Kallisto)
indexing
Kallisto
updated 7 hours ago by
dsull
★ 5.8k • written 13 hours ago by
Aaliya
▴ 10
3
votes
0
replies
79
views
News:
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
Scanpy
scRNA
Machinelearning
GenerativeAI
8 hours ago by
Claire Watson
▴ 60
0
votes
2
replies
150
views
Rare Disease Variant Pathway Analysis
Pathway
analysis
8 hours ago by
The_PyPanda
▴ 10
0
votes
0
replies
74
views
Phasing VCF Files and Analyzing Reads with Multiple Variants
haplotypes
vcf
phasing
9 hours ago by
HarperReed
• 0
3
votes
3
replies
134
views
genome assembly records not present in assembly_summary.txt
ncbi
bacteria
assembly
updated 6 hours ago by
GenoMax
141k • written 10 hours ago by
sapuizait
▴ 10
1
vote
0
replies
53
views
Simulation of label-free bottom-up proteomics expression dataset
label-free
bottom-up
lc-ms
proteomics
11 hours ago by
KABILAN
▴ 50
0
votes
0
replies
52
views
Running Phylogenetic Analysis With NCBI Genome
population
genetics
phylogenetic
species
sequencing
12 hours ago by
SineWave
• 0
0
votes
0
replies
53
views
RNA-seq: full length gene
RNA-seq
updated 2 hours ago by
Ram
43k • written 12 hours ago by
Nargis
• 0
0
votes
1
reply
80
views
gvcf joint calling
WES
GATK
VCF
gVCF
updated 14 hours ago by
Jeremy Leipzig
22k • written 14 hours ago by
zihanss
• 0
0
votes
0
replies
51
views
Haplotype Phased Assembly Contigs to Chromosome Annotations
Assembly
phased
Haplotype
Annotation
14 hours ago by
turcoa1
• 0
3
votes
3
replies
180
views
Sequence read length shorter than flow cell specification
illumina
NGS
sequencing
updated 20 hours ago by
swbarnes2
14k • written 1 day ago by
M
• 0
0
votes
0
replies
66
views
Designing single-stable RNA molecules
structure
RNA
21 hours ago by
Edna
• 0
0
votes
0
replies
77
views
How to visualize/predict the final transcript from Delly output?
WGS
DELLY
21 hours ago by
simplitia
▴ 130
0
votes
1
reply
115
views
Normalize scRNAseq data to housekeeping genes to compare several datasets
RNA-sequencing
housekeeping
Single-cell
normalization
updated 22 hours ago by
ATpoint
82k • written 23 hours ago by
AaronJaime
• 0
0
votes
2
replies
171
views
BLAST using both nucleotides and taxonomic local databases
blast
ncbi
taxid
taxonomy
updated 23 hours ago by
GenoMax
141k • written 1 day ago by
Begonia_pavonina
▴ 150
0
votes
2
replies
200
views
why renaming Idents in Seurat object doesn't work?
Seurat
RenameIdents
R
updated 20 hours ago by
Ram
43k • written 1 day ago by
Assa Yeroslaviz
★ 1.8k
1
vote
1
reply
137
views
ScRNAseq-How to correctly choose cell type marker genes
cellAssign
cell-markers
updated 22 hours ago by
Ram
43k • written 1 day ago by
Francesco
▴ 10
1
vote
5
replies
227
views
ScRNA data question
scRNA
Vlnplot
Samples
updated 3 hours ago by
bk11
★ 2.4k • written 1 day ago by
starswillfade
▴ 10
0
votes
0
replies
75
views
News:
Master Meta-analysis with R (May 13-16) - Online!
Meta-Analysis
Statistics
R
updated 22 hours ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
3
votes
4
replies
235
views
Why gatk VariantAnnotator required bam and coverage files
gatk
VariantAnnotator
23 hours ago by
QX
• 0
2
votes
4
replies
237
views
Fetch table from clinvar database according to a list of rsid
python
clinvar
perl
updated 22 hours ago by
Ram
43k • written 1 day ago by
ashaneev07
▴ 20
0
votes
2
replies
168
views
Highest variable features in single cell data
single-cell
updated 22 hours ago by
Ram
43k • written 1 day ago by
Kazo
• 0
0
votes
2
replies
208
views
PCA plot
DESeq2
PCAplot
updated 9 hours ago by
ATpoint
82k • written 1 day ago by
Aaliya
▴ 10
2
votes
2
replies
186
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 55 minutes ago by
BioinfGuru
★ 1.7k • written 1 day ago by
Ming Tommy Tang
★ 3.9k
0
votes
2
replies
218
views
Annovar using R package
Annovar
gnomAD
R
22 hours ago by
DKA
▴ 40
0
votes
0
replies
112
views
News:
8th Berlin Summer School in NGS Data Analysis - Apply Now
DNA-seq
variant-calling
RNA-seq
illumina
transcriptomics
updated 22 hours ago by
Ram
43k • written 2 days ago by
David Langenberger
11k
0
votes
11
replies
475
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
18 hours ago by
atowns21
• 0
2
votes
5
replies
278
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
11 hours ago by
Lada
▴ 30
8
votes
16
replies
774
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 3 hours ago by
bk11
★ 2.4k • written 7 weeks ago by
Sofia
• 0
2
votes
3
replies
318
views
How to establish haplotype-specific gene expression levels
RNA-seq
Haplotype
updated 5 hours ago by
dsull
★ 5.8k • written 17 days ago by
javanokendo
▴ 60
0
votes
7
replies
263
views
Low mapping rate with Salmon
RNA-seq
Salmon
Quantification
updated 2 hours ago by
GenoMax
141k • written 4 days ago by
Patadu94
• 0
4
votes
2
replies
367
views
Missing protein (VEGF-A) in String db
VGFA
stringdb
updated 18 hours ago by
shalespringer
• 0 • written 11 weeks ago by
Phenylananin
▴ 20
2
votes
6
replies
3.6k
views
Treatment VS Control in Single Cell RNAseq analysis
Single Cell
RNAseq
DE Analysis
Treatment
Control
updated 22 hours ago by
ATpoint
82k • written 3.9 years ago by
grayapply2009
▴ 280
10
votes
6
replies
9.8k
views
6 follow
CDS vs cDNA vs transcript for mapping RNA-Seq reads
Assembly
rna-seq
alignment
updated 9 hours ago by
Antonio R. Franco
★ 5.1k • written 6.0 years ago by
williamsbrian5064
▴ 510
41 results • Page
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Comment: Ideal PC configurations and operating system for bioinformatics laboratory
Comment: Convert SAM to BAM
Comment: Should I use unpaired reads from trimmomatic
Answer: Should I use unpaired reads from trimmomatic
A: Mean Variance Relationship single cell RNA-Seq Data
Answer: Blasting two protein sequences vs two nucleotide sequences
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Recent Replies
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i
by
ATpoint
82k
You can make each process use a dedicated environment, see https://www.nextflow.io/docs/latest/conda.html#use-existing-conda-environments …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★ 1.7k
Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon
by
i.sudbery
19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
Comment: genome assembly records not present in assembly_summary.txt
by
sapuizait
▴ 10
jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
Comment: ScRNA data question
by
bk11
★ 2.4k
I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
Comment: Low mapping rate with Salmon
by
GenoMax
141k
> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes? Reads are likely aligning in regions wh…
Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
cmdcolin
★ 3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq
by
pinheirofabiano
▴ 10
install.packages("pheatmap") library(pheatmap) setwd("/Users/data_analysis/results") data <- read.table(file…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
bedtools intersect \ -a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \ -b <(awk '{X=250000;P=int($2);printf("%s\t%d\…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
• 0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question
by
starswillfade
▴ 10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
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