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975 results • Page
6 of 20
Sort: Rank
Rank
Views
Votes
Replies
1
vote
2
replies
266
views
alignment result
RNA-seq
samtools
hisat2
11 days ago by
ahmad.sajad4541
• 0
0
votes
0
replies
130
views
News:
hands-on introduction to generalized linear models (GLMs) using R
Generalized-Linear-Models
GLM
R
updated 12 days ago by
Ram
43k • written 12 days ago by
carlopecoraro2
★ 2.5k
0
votes
2
replies
257
views
Wilcox test using data slot (log normalized count values) of scRNA-seq between two groups
scRNAseq
12 days ago by
mropri
▴ 150
2
votes
2
replies
261
views
HaplotypeCaller - only SNPs
HaplotypeCaller
GATK
updated 12 days ago by
analyst
▴ 30 • written 12 days ago by
lorena9132
▴ 10
0
votes
0
replies
128
views
AttributeError: module 'scvelo' has no attribute 'pp
loomfile
scvelo
scipy
annData
numpy
12 days ago by
Kash
▴ 110
7
votes
2
replies
306
views
Gene set enrichment analysis differences between 2020 and 2024
Gene-ontology
updated 12 days ago by
geneontologyhelp
▴ 400 • written 14 days ago by
catherine.teyssier
• 0
1
vote
1
reply
234
views
Blasting two protein sequences vs two nucleotide sequences
gene-hunting
blast
updated 13 days ago by
Istvan Albert
100k • written 14 days ago by
Abeer
• 0
2
votes
4
replies
364
views
Should I use unpaired reads from trimmomatic
RNA-seq
QC
Trimmomatic
updated 12 days ago by
swbarnes2
14k • written 14 days ago by
dxj294
• 0
0
votes
1
reply
177
views
How to solve this RoseTTAFold colaboratory error?
modeling
Google
Colaboratory
RoseTTAFold
protein
updated 12 days ago by
Mensur Dlakic
★ 27k • written 13 days ago by
benguyarenbeyaz98
• 0
2
votes
2
replies
317
views
How to calculate identity percentage between proteins contained in a FASTA file?
protein
FASTA
alignment
updated 12 days ago by
Ram
43k • written 13 days ago by
v.berriosfarias
▴ 140
3
votes
1
reply
258
views
Seurat merge and batch correction
Seurat
updated 13 days ago by
Ram
43k • written 13 days ago by
sooni
▴ 20
1
vote
1
reply
227
views
Herald:
The Biostar Herald for Tuesday, April 23, 2024
herald
updated 13 days ago by
Pierre Lindenbaum
161k • written 13 days ago by
Biostar
2.7k
1
vote
1
reply
173
views
Including plasmid in alignment
Bacteria
Transcriptomics
BOWTIE2
STAR
6 days ago by
heelpPlease
• 0
2
votes
9
replies
914
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 12 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
0
votes
11
replies
645
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
10 days ago by
atowns21
• 0
0
votes
1
reply
190
views
Find subcluster under a cluster, find differential genes in one cluster between 2 samples??
clustering
subclustering
scRNAseq
updated 13 days ago by
bk11
★ 2.4k • written 13 days ago by
alphaflylizard
• 0
5
votes
4
replies
378
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
4 days ago by
Estevão
• 0
0
votes
3
replies
296
views
Difference between samtools "-f 9" and "-f 11"
samtools
sam
bam
updated 13 days ago by
Istvan Albert
100k • written 13 days ago by
Dylan
• 0
2
votes
5
replies
383
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
10 days ago by
Lada
▴ 30
4
votes
11
replies
828
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 12 days ago by
LauferVA
4.2k • written 13 days ago by
dominickd
• 0
8
votes
16
replies
1.1k
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 10 days ago by
bk11
★ 2.4k • written 8 weeks ago by
Sofia
• 0
0
votes
0
replies
110
views
The concept of co-occurrence network structure
co-occurence
microbiome
network
13 days ago by
ohtang7
▴ 40
3
votes
0
replies
140
views
News:
Interested in Spatial Omics?
Bioconductor
Spatial-Omics
Spatial-Transcriptomics
updated 13 days ago by
Ram
43k • written 13 days ago by
carlopecoraro2
★ 2.5k
2
votes
2
replies
464
views
Using vg gamsort with naive sorting algorithm
gamsort
vg
updated 13 days ago by
Pierre Lindenbaum
161k • written 8 months ago by
AshleeThomson
▴ 80
0
votes
1
reply
187
views
Plot DNA methylation level of DMR on the basis of certain genes
rrbs
methylkit
updated 13 days ago by
GenoMax
142k • written 13 days ago by
Srinka
▴ 20
8
votes
5
replies
6.0k
views
ISCN annotation for SV/CN VCF files
SV
written 8 months ago by
a.beggs
▴ 60
0
votes
5
replies
389
views
Should I remove unlocalized scaffols in reference genome before alignment?
qualimap
alignment
minimap2
GRCh38
assembly
12 days ago by
njornet
▴ 20
5
votes
4
replies
697
views
How to compare the quality of assemblies
nextdenovo
assembly
hifiasm
pacbio
12 days ago by
kirillkirilenko
▴ 40
0
votes
0
replies
112
views
Software to calculate RSCU, ENC other than codonw
rscu
codonw
updated 13 days ago by
Ram
43k • written 13 days ago by
SHREYA
• 0
0
votes
3
replies
418
views
How to identify gaps in a genome?
awk
seqkit
assembly
fasta
genome
updated 13 days ago by
colindaven
6.4k • written 21 days ago by
rj.rezwan
• 0
0
votes
0
replies
115
views
Merging replicates from Encode project
CHIP-seq
encode
13 days ago by
Nurken
• 0
1
vote
3
replies
273
views
Filter BAM to keep only alignments at an interval, ignoring reads spliced over
samtools
bam
splicing
13 days ago by
WouterDeCoster
47k
0
votes
3
replies
258
views
Help for coding: Trinity for differential gene expression studies
Gene
updated 13 days ago by
mchour
• 0 • written 13 days ago by
rhossen
• 0
1
vote
1
reply
184
views
WGCNA preservation analysis
WGCNA
preservation
updated 13 days ago by
Michael
54k • written 13 days ago by
michael.flower.14
▴ 180
1
vote
3
replies
313
views
some error in building kraken2 database
metagenome
kraken2
12 days ago by
Art1ess
• 0
0
votes
1
reply
185
views
Subset Seurat object from Xenium spatial data
Seurat
Xenium
Spatial
updated 13 days ago by
bk11
★ 2.4k • written 13 days ago by
Susmita Mandal
▴ 110
0
votes
1
reply
161
views
Plots for DNA methylation data
plots
DNA
methylation
updated 13 days ago by
GenoMax
142k • written 13 days ago by
sarahawan92
▴ 10
0
votes
3
replies
537
views
python file for coding potential calculator
cpc2.py
updated 13 days ago by
atharvakarkare14
▴ 30 • written 18 days ago by
Ashok
• 0
1
vote
1
reply
199
views
Failed kmer content
kmer
illumina
ngs
updated 13 days ago by
Ram
43k • written 13 days ago by
Kasturi
• 0
0
votes
0
replies
179
views
Forum:
Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation
scRNA
llm
13 days ago by
yxwucq
• 0
0
votes
0
replies
103
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
HMMcopy
Copy-number-analysis
R
updated 13 days ago by
Ram
43k • written 13 days ago by
SSSJec
• 0
0
votes
6
replies
400
views
How to slice a gvcf file with tabix?
Tabix
bed
gvcf
13 days ago by
Sd
• 0
0
votes
2
replies
206
views
Help with Base quality Score Recalibration (BQSR) using GATK
GATK
BQSR
14 days ago by
sainavyav22
• 0
0
votes
1
reply
165
views
Identification of Transgene insertion sites
Transgene
Whole-Genome-Sequencing
transgene-sites
updated 14 days ago by
Ram
43k • written 14 days ago by
Cameron.walker9900
• 0
0
votes
8
replies
404
views
Error in cnetplot enrichplot package
R
updated 14 days ago by
Ram
43k • written 14 days ago by
Farhad
• 0
3
votes
9
replies
549
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 12 days ago by
Istvan Albert
100k • written 14 days ago by
Κοσμάς
• 0
0
votes
0
replies
119
views
Pairwise alignment and Ka/Ks computation
Pairwise-Alignment
Genomics
Ka-Ks
updated 14 days ago by
Ram
43k • written 14 days ago by
maxime.policarpo
▴ 200
3
votes
3
replies
231
views
Genbank vs SRA
ncbi
genbank
genomics
sra
updated 14 days ago by
Mensur Dlakic
★ 27k • written 14 days ago by
c_u
▴ 520
0
votes
0
replies
105
views
Analysis of the gene family of a genome.
Genome
gene-family
updated 14 days ago by
Ram
43k • written 14 days ago by
sansan_96
▴ 80
0
votes
0
replies
108
views
File with NCBI summary or Uniprot description
annotation
visualisation
NCBI
Uniprot
RNA
updated 13 days ago by
Ram
43k • written 14 days ago by
Amélie
• 0
975 results • Page
6 of 20
Recent Votes
Comment: Which hg38 file?
Answer: Which hg38 file?
Answer: Where to get the following bed file?
How to save GRanges object to csv file
Answer: How to get proteins from GFF file resulted from MAKER annotation
Answer: Gene ontology and homologs
Comment: anRichment is missing
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Recent Replies
Comment: Where to get the following bed file?
by
GenoMax
142k
SeqCap kits are discontinued. If you don't find the said file your will need to email Roche Support per this page: https://sequencing.roche…
Answer: Where to get the following bed file?
by
LChart
3.9k
UCSC maintains a list of exome probe sets and targets. You could check there: https://hgdownload.soe.ucsc.edu/gbdb/hg19/exomeProbesets/
Comment: How to compute TPM normalized values for TCGA miRNA data?
by
Ngrin
• 0
Yes, I found some similar ideas on biostar repository. So you think the authors have done something wrong? And I should only consider CPM?
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
Chris Dean
▴ 390
It is difficult to answer how they were able to identify *S. hominis* without seeing the code or paper. To address your second question, i…
Comment: Problem with Seurat package in PercentageFeatureSet function
by
bk11
★ 2.4k
Did you check where the `features.tsv.gz` file had Gene IDs or Ensembl IDs?
Answer: How to compute TPM normalized values for TCGA miRNA data?
by
dsull
★ 6.0k
TPM is irrelevant for miRNA-seq; CPM works fine. TPM tries to adjust for length effects by dividing by gene length (e.g. reducing the im…
Comment: Applying the metacell2 algorithm using python
by
Wayne
★ 2.0k
"I'm also unsure what qualifies as a lateral gene." The [README where you reference](https://metacells.readthedocs.io/en/latest/readme.htm…
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
Ah, yes this makes sense! I knew this was an issue with, say, using kraken2 on shotgun metagenomic reads, but I don't have a lot of experi…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴ 40
Thank you. Maybe it's best to confirm with the authors about how they're getting responder/non-responder labels. I'll do that now.
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
Chris Dean
▴ 390
If you sequenced a short hypervariable region, most of your sequence reads will not be able to be assigned to the species level. One possi…
Comment: anRichment is missing
by
GenoMax
142k
Consider emailing Dr. Horvath about this. Until enough people make him aware of this issue WGCNA "problem" is unlikely to get fixed.
Comment: anRichment is missing
by
Tatyana
• 0
Unfortunately, there are only tutorials and codes for tutorials for WGCNA, not installation files for any their packages... :(
Comment: anRichment is missing
by
GenoMax
142k
See this link for the WGCNA documentation and code from Peter Langfelder : https://bioinformatics.stackexchange.com/questions/21885/where-t…
Comment: anRichment is missing
by
Tatyana
• 0
The problem is back, Horwath's lab web is down again. Is it possible to get anRichment istallation file somewhere? (The greater problem is …
Comment: 16S rRNA microbiome differential abundance analysis with DESeq2 finds same micro
by
andres.firrincieli
3.6k
1. Maribaculaceae is a family not a genus 2. Who said that ASV with the same taxonomic classification must have the same behaviour? 3. With…
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