Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Post does not exist.
Limit : all time
all time
today
this week
this month
this year
121,440 results • Page
1 of 2429
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
16
views
News:
New Bioconductor and Physalia Collaboration!
Bioconductor
DataAnalysis
Genomics
1 hour ago by
Physalia-courses
★ 2.6k
1
vote
1
reply
117
views
Read1 and read2 asymmetry in stranded, paired-end rnaseq data aligned by STAR
stranded
STAR
RNAseq
antisense
sense
updated 3 hours ago by
rfran010
★ 1.5k • written 1 day ago by
yampolsk
▴ 10
0
votes
1
reply
114
views
Does the order of SplitNCigarReads and MarkDuplicates affect RNA-seq variant calling results?
variantcalling.
rnaseq
gatk
updated 3 hours ago by
rfran010
★ 1.5k • written 1 day ago by
iamsmor
• 0
0
votes
0
replies
30
views
Should I standardize age and age2 as covariates in GWAS models?
GWAS
covariates
4 hours ago by
yu
• 0
3
votes
5
replies
340
views
Venn Diagram
VennDiagram
updated 6 hours ago by
jianhua.mert
• 0 • written 2 days ago by
Nasim Gandomdoust
• 0
840
votes
170
replies
180k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 8 weeks ago by
Biostar
3.6k • written 8.6 years ago by
Istvan Albert
102k
0
votes
0
replies
50
views
PhageTerm error
phage
genome
phageterm
assamble
8 hours ago by
NIkita
• 0
0
votes
1
reply
72
views
WGCNA Workflow from GEO FASTQ Files – Expression Type, Sample Inclusion, and Preprocessing
fastq
wgcna
rna
updated 8 hours ago by
GenoMax
152k • written 10 hours ago by
tutorwhizzz
• 0
0
votes
0
replies
53
views
small RNA-Seq pipeline Standstill
NGS
miRNA
10 hours ago by
anthony.santana.703.j
▴ 30
0
votes
2
replies
110
views
comparison interpretation
model
design
contrast
matrix
updated 3 hours ago by
rfran010
★ 1.5k • written 15 hours ago by
jkim
▴ 190
0
votes
0
replies
51
views
piRNAs Analysis Pipelines
piRNAs
smallRNA
13 hours ago by
GA
• 0
0
votes
1
reply
64
views
ATAC-seq analysis
samtools
ATAC-seq
bowtie2
deeptools
updated 2 hours ago by
ATpoint
88k • written 14 hours ago by
SEJAL
• 0
1
vote
1
reply
85
views
Beagle PATH (partially) recognised on Ubuntu 22.04
Ubuntu
Beagle
Beast
MCMC
updated 16 hours ago by
GenoMax
152k • written 17 hours ago by
Luca Arbore
▴ 10
2
votes
2
replies
127
views
custom BED to UCSC genome browser
browser
ucsc
ucsc-genome-browser
genome
methylation
bed
updated 19 hours ago by
Maximilian Haeussler
★ 1.7k • written 23 hours ago by
npont
▴ 10
0
votes
0
replies
77
views
CNV Computation with HoneyBadger
CNV
HoneyBADGER
updated 16 hours ago by
GenoMax
152k • written 23 hours ago by
mete.han.celebi
• 0
0
votes
0
replies
73
views
How to predict programatically TFBS and calculate PWM Score as JASPAR
PWMScan
TFBS
JASPAR
1 day ago by
t.kajiyama
• 0
0
votes
0
replies
115
views
News:
Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introduction (September 10-12, 2025 in Berlin, Germany)
Workshop
RNA-Seq
Illumina
VariantCalling
DNA-Seq
1 day ago by
ecSeq Bioinformatics
▴ 20
0
votes
14
replies
517
views
Host Removal Issues Human/Dog with samples containing Eukaryotic species
Eukarotic
Mapping
Host
updated 1 day ago by
Mensur Dlakic
★ 29k • written 2 days ago by
Jon
• 0
0
votes
0
replies
85
views
Computing ka/ks ratio for individual genes.
ks
ka
evolution
selection
1 day ago by
Shakunthala Natarajan
• 0
1
vote
1
reply
165
views
Pharokka Error
error
annotating
viral
pharokka
genome
updated 1 day ago by
Mensur Dlakic
★ 29k • written 1 day ago by
NIkita
• 0
0
votes
0
replies
100
views
Combining Metatranscriptomics (Mtx) and Metagenomics (Mgx)
metagenomics
reference-based
Metatranscriptomics
alignment
updated 1 day ago by
GenoMax
152k • written 1 day ago by
rk009
• 0
1
vote
1
reply
159
views
how to make a circular plot of variants
circular
plot
updated 1 day ago by
Pierre Lindenbaum
166k • written 1 day ago by
analyst
▴ 70
2
votes
6
replies
296
views
GSEA analysis
RNA-seq
RNA
analysis
updated 1 day ago by
GenoMax
152k • written 2 days ago by
Ahmed
• 0
0
votes
0
replies
101
views
how to make go term plot and kegg pathway plot using InterProScan output
interproscan
2 days ago by
analyst
▴ 70
1
vote
3
replies
434
views
Why little difference in RNAseq alignment when flipping forward and reverse strand with paired end reads
mRNA
rna-seq
bowtie
alignment
updated 2 days ago by
swbarnes2
15k • written 3 days ago by
JourneyToAbyss
▴ 250
0
votes
2
replies
213
views
DESeq2 design for differential expression with 2 timepoints and 2 controls
Transcriptomic
Feeding
DESeq2
RNAseq
updated 14 hours ago by
jkim
▴ 190 • written 2 days ago by
Cristina
• 0
0
votes
1
reply
188
views
Questions about sample_accession_id and file_accession_id from EGA files
EGA
open
WGS
data
updated 2 days ago by
GenoMax
152k • written 3 days ago by
askif4
▴ 20
4
votes
3
replies
314
views
Example of ClinVar pathogenic variant where the reference allele is pathogenic?
snp
variant
pathogenic
clinvar
updated 3 days ago by
GenoMax
152k • written 3 days ago by
a615ebfb
▴ 60
0
votes
1
reply
202
views
Functional Analysis Using EMU Output – Has Anyone Tried This?
emu
updated 3 days ago by
andres.firrincieli
3.9k • written 3 days ago by
henrique.mouradias
• 0
1
vote
1
reply
206
views
featureCounts -t option not working in v2.0.8?
subread
featureCounts
RNAseq
updated 3 days ago by
rfran010
★ 1.5k • written 3 days ago by
atan
• 0
0
votes
1
reply
208
views
mm10 blacklist regions detailed information
sequencing
DNA
blacklist
updated 3 days ago by
GenoMax
152k • written 3 days ago by
annaA
▴ 10
0
votes
2
replies
242
views
identify unmapped regions
human
illumina
libraries
1 day ago by
María José
▴ 10
0
votes
1
reply
338
views
martCheck - Error - You must provide a valid Mart object - for running HoneyBADGER for CNV computation
CNV
biomaRt
HoneyBADGER
3 days ago by
mete.han.celebi
• 0
0
votes
1
reply
254
views
How to Specify Power Parameter for Adjacency in modulePreservation() (WGCNA)
module
analysis
WGCNA
preservation
updated 3 days ago by
andres.firrincieli
3.9k • written 3 days ago by
Berkan
• 0
0
votes
1
reply
226
views
filters used in mutation discovery from RNAseq data
filter
RNAseq
mutation
updated 3 days ago by
GenoMax
152k • written 4 days ago by
htchd
▴ 20
2
votes
4
replies
371
views
Trouble Using GEO Files with Seurat
seurat
GEO
scRNA
updated 2 hours ago by
antonioggsousa
3.4k • written 5 days ago by
Jamie
• 0
0
votes
0
replies
207
views
Knowledge Graph for AI
KnowledgeGraph
biotech
AI
SPOKE
Bioinformatician
4 days ago by
Cornelio
• 0
2
votes
2
replies
937
views
How to convert from .spec to .bam?
bam
file-formatting
spec
updated 4 days ago by
Daniel Lai
▴ 10 • written 4.8 years ago by
gmora
▴ 30
2
votes
1
reply
258
views
Insert size is zero
rna-seq
fastp
insert
size
updated 4 days ago by
GenoMax
152k • written 4 days ago by
SpliceAndScript
• 0
0
votes
3
replies
367
views
Should matched samples (not paired) be included in the DESeq2 design model?
edgeR
limma
DESeq2
updated 3 days ago by
i.sudbery
21k • written 5 days ago by
marieke
• 0
0
votes
4
replies
367
views
Trajectory Analysis scRNA-seq with one cell type and two developmental stages
cellrank
palantir
pseudotime-trajectory-analysis
scrna-seq
trajectory-analysis
4 days ago by
npont
▴ 10
0
votes
1
reply
600
views
How to run trajectory analysis with only one cell type using palantir?
palantir
scanpy
updated 5 days ago by
npont
▴ 10 • written 11 months ago by
bioinfo
▴ 160
0
votes
1
reply
246
views
should I remove the transcripts from annotate peak region in ATAC-seq
ATAC-seq
updated 3 days ago by
rfran010
★ 1.5k • written 5 days ago by
QX
▴ 70
3
votes
2
replies
339
views
DESeq2 LRT test with multifactorial design
test
design
multifactorial
LRT
5 days ago by
lessismore
★ 1.4k
1
vote
0
replies
981
views
Tool:
Created a better alternative to clunky gene databases, with functions that are consolidated from various gene databases. It's your one-stop shop for …
browser
export
data
search
gene
5 days ago by
Krish
• 0
1
vote
0
replies
224
views
Tool:
Containerized NGS Data Processing Pipelines
rna-seq
atac-seq
pipeline
chip-seq
docker
5 days ago by
Noah
▴ 10
0
votes
1
reply
257
views
Scvi - Integration
integration
singlecell
scvi
updated 5 days ago by
antonioggsousa
3.4k • written 5 days ago by
t.foskolos
• 0
0
votes
0
replies
223
views
Genotype Imputations Question
vcf
imputations
genomics
5 days ago by
Jonathan
• 0
0
votes
1
reply
270
views
Clarification of options --keep-dup: all vs. -f FRAG in MACS3
human
libraries
illumina
callpeak
macs3
updated 5 days ago by
ATpoint
88k • written 5 days ago by
María José
▴ 10
10
votes
4
replies
608
views
Should I perform integration to correct batch effects?
batch
effect
updated 2 hours ago by
antonioggsousa
3.4k • written 10 days ago by
han
▴ 30
121,440 results • Page
1 of 2429
Recent Votes
Find structural variants comparing two assemblies
Answer: Read1 and read2 asymmetry in stranded, paired-end rnaseq data aligned by STAR
Answer: custom BED to UCSC genome browser
How to use FindSubCluster in Seurat?
How to combine multiple pheatmap figures and use common legend?
A: How to combine multiple pheatmap figures and use common legend?
A: Sequence length from Fasta
Recent Locations •
All
Oviedo,
11 minutes ago
UCLA,
12 minutes ago
Pakistan,
15 minutes ago
Sheffield, UK,
27 minutes ago
United Kingdom,
28 minutes ago
Canada,
31 minutes ago
Italy,
31 minutes ago
Recent Awards •
All
Popular Question
to
Jeremy Leipzig
23k
Popular Question
to
questionaire
• 0
Teacher
to
LChart
5.0k
Popular Question
to
ja569116
• 0
Popular Question
to
Apprentice
▴ 170
Commentator
to
lieven.sterck
15k
Student
to
han
▴ 30
Recent Replies
Comment: Trouble Using GEO Files with Seurat
by
antonioggsousa
3.4k
I do not know if they are exactly the same, but I think they are equivalent from its description ([link](https://anndata.readthedocs.io/en/…
Comment: Should I perform integration to correct batch effects?
by
antonioggsousa
3.4k
You are welcome. I have to be completely honest and say that I do not have experience with other modalities rather than scRNA-seq. Though c…
Comment: ATAC-seq analysis
by
ATpoint
88k
https://www.biostars.org/p/357878/ Mind the details and effort part.
Answer: Read1 and read2 asymmetry in stranded, paired-end rnaseq data aligned by STAR
by
rfran010
★ 1.5k
A. may be the most correct, but I think you are making some incorrect assumptions about READ1 and READ2, so that I don't think any of these…
Comment: comparison interpretation
by
rfran010
★ 1.5k
To maybe add more context, Drug at 0h and Placebo at 0h should be equivalent samples, since they are effectively both "untreated". However…
Answer: Does the order of SplitNCigarReads and MarkDuplicates affect RNA-seq variant cal
by
rfran010
★ 1.5k
I have not compared results as you suggest, but logically, there is a functional difference. Whether this has a great effect depends on the…
Answer: Venn Diagram
by
jianhua.mert
• 0
stop using venn diagram. Use Upset plot instead. There are some upset plots in journals at https://plottie.art/search?q=upset&loaded_pages=…
Comment: WGCNA Workflow from GEO FASTQ Files – Expression Type, Sample Inclusion, and Pre
by
GenoMax
152k
> Use of fastp: Even if no adapters are detected, would you still recommend using fastp for consistency across datasets? No harm in using …
Comment: Venn Diagram
by
jared.andrews07
★ 19k
7 is the max I've seen, though even [6 sets usually starts to look pretty stupid](https://www.nature.com/articles/nature11241/figures/4). I…
Answer: comparison interpretation
by
Mensur Dlakic
★ 29k
Both the drug and placebo must be brought to the common frame of reference (0h) before they can be compared to each other. If you did `Drug…
Comment: DESeq2 design for differential expression with 2 timepoints and 2 controls
by
jkim
▴ 190
Have a look at [the contrast and interaction section](https://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#in…
Answer: Beagle PATH (partially) recognised on Ubuntu 22.04
by
GenoMax
152k
> I can run BEAST only in the same terminal window if I have have executed the export PATH command first. If I open a new terminal and try …
Answer: custom BED to UCSC genome browser
by
Maximilian Haeussler
★ 1.7k
Hi, we just added bedMethyl support! Try it at https://genome-test.gi.ucsc.edu/cgi-bin/hgTables. type must be "bedMethyl". Please let us kn…
Answer: custom BED to UCSC genome browser
by
barslmn
★ 2.4k
BED files have specific format. https://genome.ucsc.edu/FAQ/FAQformat.html#format1 UCSC uses these columns to display the tracks. Your fil…
Comment: Should I perform integration to correct batch effects?
by
han
▴ 30
Thank you very much for your detailed and thoughtful response! It was very helpful and clarified several important points regarding integra…
Traffic: 3668 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6