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116,970 results • Page
1 of 2340
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13
views
Blog:
The Current State and Future Prospects of Long-Read Sequencing
LRS
25 minutes ago by
Novogene
▴ 420
0
votes
0
replies
12
views
Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
build
genome
29 minutes ago by
Ali
• 0
0
votes
0
replies
16
views
qPCR gene expression data analysis
qPCR
geneexpression
anova
1 hour ago by
ebertomeup
• 0
0
votes
3
replies
236
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
just now by
egascon
• 0
0
votes
1
reply
31
views
Sam file Header problem
Sam
Header
problem
file
updated 1 hour ago by
Pierre Lindenbaum
161k • written 2 hours ago by
saifulislam99121
• 0
0
votes
4
replies
143
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
Degrees
python
Degree
offtopic
2 hours ago by
kuttibiotech2009
▴ 30
0
votes
1
reply
485
views
scRNAseq quality control weird double curve in nFeature vs nCount plot
scRNA-seq
QC
single
cell
BDRhapsody
analysis
updated 3 hours ago by
valdirbarth
▴ 20 • written 12 months ago by
Salomé
• 0
0
votes
0
replies
38
views
select set of intervals that cover a genomic region
GRanges
bed
4 hours ago by
ntsopoul
▴ 60
1
vote
0
replies
43
views
Tool:
Collect dispersed executable files and run them by using bingo.
bingo
4 hours ago by
dwpeng
▴ 10
0
votes
0
replies
36
views
BiomartException: Query ERROR for existing dataset in BioMart
biomart
ensembl
wheat
python
pybiomart
6 hours ago by
Luqman
• 0
797
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.8k • written 7.4 years ago by
Istvan Albert
100k
0
votes
2
replies
79
views
ggrepel function: geom_text_repel()
ggrepel
ggplo2
R
updated 58 minutes ago by
zx8754
11k • written 6 hours ago by
sooni
▴ 20
0
votes
2
replies
83
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
1 hour ago by
mavy
▴ 10
0
votes
1
reply
80
views
How to extract cells of different species after mapping with combined genome?
snrnaseq
scrnaseq
updated 6 hours ago by
GenoMax
142k • written 10 hours ago by
vk
▴ 10
1
vote
1
reply
163
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
11 hours ago by
ramiro.barrantes
• 0
1
vote
1
reply
125
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
updated 11 hours ago by
marco.barr
▴ 120 • written 16 hours ago by
diqixiaoyaoer
▴ 10
2
votes
6
replies
1.8k
views
How can you *consistently* download BioProject IDs from NCBI's BioSample database using Entrez Direct?
XML
ncbi
entrez-direct
updated 11 hours ago by
Ram
43k • written 3.9 years ago by
millere
• 0
0
votes
0
replies
64
views
What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
12 hours ago by
mgranada3
▴ 30
0
votes
0
replies
59
views
Add line under stat test
stats
R
12 hours ago by
Ghada
• 0
1
vote
5
replies
748
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
written 6 months ago by
Daniel
▴ 30
0
votes
0
replies
60
views
DOCK6 installation on MacOS M1
installation
troubleshooting
DOCK6
updated 14 hours ago by
GenoMax
142k • written 14 hours ago by
Rodolfo Adrián
• 0
1
vote
3
replies
147
views
Genotyping sites with N in reference genome
GATK
UnifiedGenotyper
updated 14 hours ago by
Zhenyu Zhang
★ 1.2k • written 15 hours ago by
shpak.max
▴ 50
0
votes
1
reply
106
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 15 hours ago by
yura.grabovska
▴ 70 • written 15 hours ago by
ev97
▴ 20
0
votes
6
replies
176
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
meta
how-to
ncbi
forum
19 minutes ago by
hophuquy0944
• 0
0
votes
4
replies
175
views
Sequencing Depth (Read Depth) Calculations
depth
5 hours ago by
LucisTheFather
• 0
0
votes
1
reply
95
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
15 hours ago by
asalimih
▴ 60
0
votes
0
replies
69
views
Is it okay to assemble my 12 metatranscriptome samples with Trinity separately?
trinity
metatranscriptome
16 hours ago by
jway
• 0
2
votes
2
replies
132
views
Cannot install bwa-mem2 via conda
conda
mamba
python
code
updated 16 hours ago by
Istvan Albert
100k • written 17 hours ago by
jsmith120f
• 0
2
votes
2
replies
155
views
genome finishing
finishing
genome
updated 17 hours ago by
nd48
▴ 20 • written 21 hours ago by
trezini
• 0
1
vote
5
replies
173
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 17 hours ago by
Pierre Lindenbaum
161k • written 18 hours ago by
biology_inform
▴ 50
1
vote
4
replies
238
views
Add stats to boxplot in R
stats
R
14 hours ago by
Ghada
• 0
0
votes
0
replies
82
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 17 hours ago by
ATpoint
82k • written 18 hours ago by
yura.grabovska
▴ 70
0
votes
0
replies
73
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
18 hours ago by
Lélé
▴ 10
0
votes
3
replies
169
views
calculating genomic coverage/ base overlap in R
bioinoformatics
genomics
updated 14 hours ago by
marco.barr
▴ 120 • written 20 hours ago by
Sayantani
• 0
0
votes
3
replies
142
views
Annotating file using bcftools
annotation
plink
bcftools
9 hours ago by
kl
▴ 10
0
votes
9
replies
284
views
Duplicated reads (IDs) from nanopore sequencing
bam
nanopore
sequencing
1 hour ago by
njornet
▴ 20
0
votes
3
replies
128
views
Problem with Calling Variants from RNA-Seq data
vcf
gatk
benchmark
giab
rna-seq
21 hours ago by
Esraa
• 0
1
vote
1
reply
119
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
updated 22 hours ago by
ATpoint
82k • written 23 hours ago by
Alexandra
• 0
1
vote
1
reply
195
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 23 hours ago by
dariober
14k • written 2 days ago by
Holly
▴ 10
1
vote
4
replies
243
views
Filtering VCF files based on VAF giving incorrect results
bcftools
GATK
Bioinformatics
picard
VCF
10 hours ago by
Arton
▴ 10
0
votes
0
replies
80
views
ReactomeGSA
ReactomeGSA
1 day ago by
Shaimaa Gamal
▴ 10
0
votes
0
replies
75
views
News:
New course: Manipulation of NGS Data for Genomic and Population Genetics Analyses
KeepLearning
TScourses
1 day ago by
Transmitting Science communication
▴ 70
0
votes
5
replies
254
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 6 hours ago by
GenoMax
142k • written 1 day ago by
chrisk
• 0
0
votes
1
reply
122
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 18 hours ago by
Joe
21k • written 1 day ago by
iqra
• 0
0
votes
0
replies
88
views
Why the shape of RCS is contradictory to the p value for nonlinearity.
RCS
ggplot2
ggplot
R
updated 1 day ago by
GenoMax
142k • written 1 day ago by
Dude
• 0
0
votes
0
replies
84
views
NEURON, Brian2, NEST, or PyNN
NEST
NEURON
PyNN
Brian2
1 day ago by
brunofelicianodeomena
• 0
1
vote
2
replies
181
views
Alphafold 3 pLDDT coloring scheme in chimerax?
ChimeraX
Alphaphold
Alphaphold3
updated 1 day ago by
colindaven
6.4k • written 1 day ago by
mbrav005
• 0
0
votes
1
reply
137
views
Polish a large genome with Pilon
Pilon
Pacbio
Polish
Illumina
1 day ago by
sansan_96
▴ 90
1
vote
2
replies
201
views
Doubt about the process of annotation, detection, identification and classification of transposable elements
annotation
TransposableElements
11 hours ago by
MarcosCosta
• 0
2
votes
2
replies
191
views
Genome Visualization Tools
bacterial
genome
updated 1 day ago by
GenoMax
142k • written 1 day ago by
dlera.lozano
▴ 10
116,970 results • Page
1 of 2340
Recent Votes
Comment: PyWGCNA
PyWGCNA
Collect dispersed executable files and run them by using bingo.
A: ensembl gene id
A: How to deal with the case that one gene symbol matches multiple ensembl ids?
Answer: DESeq2 error
Answer: Using ggplotly in R
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Recent Replies
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
hophuquy0944
• 0
the nt database was extracted, and this is latest version of nt database in ncbi and blastn with version 2.15. I used many query file.fast…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
hophuquy0944
• 0
they don't have clear answer for this problem. I run all subset of this data to find out error subsets database but it seem not any error i…
Comment: ggrepel function: geom_text_repel()
by
zx8754
11k
There is a dedicated package for this, see - https://bioconductor.org/packages/release/bioc/html/EnhancedVolcano.html
Comment: Sam file Header problem
by
Pierre Lindenbaum
161k
-R "@RG\tID:sample\tSM:sample\tPL:Illumina" where is this '@RG' in the header anyway ? are you sure you're handling the correct fil…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
Pierre Lindenbaum
161k
it looks like the same error.
Comment: how to treat the replicates while performing WGCNA
by
mavy
▴ 10
Thankyou so much Andres for your reply. I think I got your point, just have a couple of quick questions to make it more clear . 1) I do n…
Comment: Duplicated reads (IDs) from nanopore sequencing
by
njornet
▴ 20
I've looked into it and one of the reads with a supplementary alignment has the representative alignment aligning to the + strand and being…
Answer: Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
by
kuttibiotech2009
▴ 30
Hello friends, Thank you for your feedback. I understand that the post may not directly relate to bioinformatics. My aim was to share …
Comment: how to treat the replicates while performing WGCNA
by
andres.firrincieli
3.6k
You do not merge the MEs. It is quite normal that some modules have noisy eigengenes (MEs); WGCNA is an unsupervised analysis overall. By …
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
hophuquy0944
• 0
my blast is latest version 2.15. I don't know what you want me to see in this link.
Answer: scRNAseq quality control weird double curve in nFeature vs nCount plot
by
valdirbarth
▴ 20
Is it possible that the cells that have their VDJ sequenced, those VDJ reads are not contributing to new genes detected? So you might be …
Comment: ggrepel function: geom_text_repel()
by
ntsopoul
▴ 60
The plot looks confusing, chek the documentation there is an attribute called force. If you reduce the value you should get better results.…
Comment: Sequencing Depth (Read Depth) Calculations
by
LucisTheFather
• 0
Understood! Gonna try those two!
Comment: Sequencing Depth (Read Depth) Calculations
by
LucisTheFather
• 0
Thank u very much!
Comment: How to extract cells of different species after mapping with combined genome?
by
GenoMax
142k
You have used the tag `snRNAseq`. I am not familiar with singleron tech but if you have sequence data you may be able to use `xenome` or `b…
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