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116,887 results • Page
1 of 2338
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Rank
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Votes
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1
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414
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Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 3 hours ago by
arctic
▴ 40 • written 9 days ago by
M.
▴ 30
0
votes
0
replies
32
views
Manual Assembly and Protein Translation, HELP, assignment revision
sequence
university
assembly
protein
genomics
5 hours ago by
rackbersingh
• 0
0
votes
1
reply
95
views
How to remove multiple batch effects from RNA-seq data before limma differential gene expression analysis?
batch
ComBat-seq
limma
RNA-seq
removeBatchEffect
updated 1 hour ago by
ATpoint
82k • written 5 hours ago by
t.fortunato.asquini
• 0
0
votes
1
reply
149
views
Practical Haplotype Graph v2 not finding correct paths
Pangenome
PHG
graph
updated 6 hours ago by
pjb39
▴ 200 • written 2 days ago by
beantkapoor16
• 0
795
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
0
votes
2
replies
115
views
Output file of satools flagstat empty
samtools-flagstat
updated 7 hours ago by
Mathew
▴ 100 • written 8 hours ago by
ramendra.sarma
• 0
0
votes
0
replies
44
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
eigencorplot
PCAtools
deseq2
pca
8 hours ago by
BioinfGuru
★ 1.7k
2
votes
8
replies
204
views
joint callset and vcf sorting, unknown TAG issue
sort
bcftools
GLNexus
merge
VCF
updated 7 hours ago by
Pierre Lindenbaum
161k • written 11 hours ago by
Matteo Ungaro
▴ 100
0
votes
0
replies
64
views
News:
Online course: An Introduction to Nanopore Direct RNA Sequencing
Nanopore
RNAseq
14 hours ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
167
views
input file in rmats
rmats
updated 16 hours ago by
Mathew
▴ 100 • written 2 days ago by
Lambodarswain316
• 0
0
votes
1
reply
172
views
Kraken2 database
kraken
microbialdb
database
krakendb
kraken2
updated 23 hours ago by
Mathew
▴ 100 • written 1 day ago by
Christopher
• 0
6
votes
7
replies
255
views
Longest transcript variant per gene
transcript
longest
variant
orthofinder
2 hours ago by
sansan_96
▴ 90
2
votes
2
replies
156
views
error in fun(x((i)), ...) : only defined on a data frame with all numeric alike variables
studio
Cibersort
R
updated 1 day ago by
Mathew
▴ 100 • written 1 day ago by
Azra
▴ 10
2
votes
1
reply
164
views
Failed to open VCF file
GATK
sentieon
BWA-MEM
updated 1 day ago by
Pierre Lindenbaum
161k • written 1 day ago by
melissachua90
▴ 70
9
votes
7
replies
7.1k
views
Align paired and unpaired reads simultaneously using Bowtie2?
alignment
updated 1 day ago by
Ruqaiya
• 0 • written 5.7 years ago by
xiaozhongzhiping
▴ 20
0
votes
2
replies
219
views
Adata.raw.X in LIANA, something wrong with conversion from Seurat to adata in python.
k
10 hours ago by
beginner123
• 0
6
votes
4
replies
13k
views
Trimmomatic: What is the difference between paired and unpaired output files in paired-end mode
trimmomatic
paired-end
updated 1 day ago by
Ruqaiya
• 0 • written 6.4 years ago by
dllopezr
▴ 130
0
votes
0
replies
93
views
imputation through beagle
panel
beagle
reference
imputation
1 day ago by
analyst
▴ 50
0
votes
1
reply
132
views
java.nio.bufferunderflowexception haplotypecaller error for bqsr reads
deducplicated
java.nio.bufferunderflowexception
haplotypecaller
bqsr
updated 1 day ago by
Pierre Lindenbaum
161k • written 1 day ago by
analyst
▴ 50
0
votes
6
replies
299
views
Is it Possible to "Merge" data from Replicates within a VCF SNP File
snp
vcf
plink
bioinformatics
genomics
updated 4 hours ago by
Pierre Lindenbaum
161k • written 1 day ago by
ajbarrett98
• 0
0
votes
11
replies
397
views
In IGV is this a heterogeneous mutation or false call?
mutations
IGV
heterogeneous
2 hours ago by
Tuck898
• 0
1
vote
3
replies
194
views
Tassel 5 GBS: Is there something wrong with my fastqs?
tassel
fastq
gbs
updated 1 day ago by
GenoMax
142k • written 1 day ago by
meck
• 0
3
votes
3
replies
206
views
what is another word for a BLAST "hit"?
blast
alignment
2 days ago by
dec986
▴ 380
1
vote
2
replies
203
views
Genome Assembly task + Protein Translation, assignment advice on a question
sequence-analysis
protein-synthesis
1 day ago by
rackbersingh
• 0
0
votes
1
reply
152
views
extract viral protein of interest from 10k whole viral genomes
nBLAST
viral-genome
updated 2 days ago by
Ram
43k • written 2 days ago by
Shwetha
• 0
0
votes
0
replies
120
views
Marker Features variance by cluster, sample, and treatment group
Seurat
scATAC-seq
ArchR
updated 2 days ago by
Ram
43k • written 2 days ago by
naomiboldon
• 0
1
vote
3
replies
224
views
How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
gatk
vcf
liftover
updated 2 days ago by
Ram
43k • written 2 days ago by
Omics data mining
▴ 260
9
votes
6
replies
12k
views
samtools tview symbols
samtools
updated 8.8 years ago by
Ashutosh Pandey
12k • written 8.8 years ago by
biolab
★ 1.4k
1
vote
7
replies
535
views
Filtering Multi-sample VCF file for all except one Genotype
variant
SNP
VCF
updated 2 days ago by
Pierre Lindenbaum
161k • written 5 days ago by
schmince
• 0
0
votes
5
replies
254
views
Too many unpaired forward reads found by Trimmomatic
Trimmomatic
2 days ago by
SilhouetteQ
• 0
0
votes
1
reply
143
views
Alignment of samples with spike-in
alignment
multimapping
RNA-seq
ChIP-seq
spike-in
updated 2 days ago by
GenoMax
142k • written 2 days ago by
maria.soler
• 0
0
votes
2
replies
230
views
How are score_weights calculated in this code?
single-cell
1 day ago by
carolofharvest
▴ 40
0
votes
1
reply
182
views
How to convert Haps file to vcf file?
pre-phasing
GWAS
imputation
updated 2 days ago by
curious
▴ 750 • written 2 days ago by
SeoGyun
• 0
2
votes
12
replies
2.7k
views
6 follow
CNVKIT - unable to produce scatter and diagram pdfs
cnvkit
updated 4 days ago by
Anitha
• 0 • written 4.8 years ago by
ww22runner
▴ 60
0
votes
0
replies
124
views
Trouble converting tfam and tped to map and ped files
plink
ped
tfam
tped
map
2 days ago by
Samantha
• 0
0
votes
1
reply
158
views
Microbial Signal Transduction Database
MiST
updated 2 days ago by
zx8754
11k • written 2 days ago by
Shravani
• 0
0
votes
1
reply
171
views
DESeq2 error
DESeq2
updated 2 days ago by
ATpoint
82k • written 2 days ago by
sooni
▴ 20
0
votes
0
replies
116
views
Allele specific expression of imprinted gens from 10x scRNA-seq data
scrna-seq
imprinted
10x
genes
2 days ago by
singcell
• 0
0
votes
1
reply
180
views
When should I use R-MarkDown over R-Script ?
r
rscript
rmarkdown
updated 2 days ago by
ATpoint
82k • written 2 days ago by
Amr
▴ 160
0
votes
2
replies
190
views
LncRNA Nomenclature
ENST
nomenclature
lncrna
GBB
ENSG
2 days ago by
jain72744
▴ 10
0
votes
0
replies
123
views
Nomalization - TCGA, RNA-seq and Microarray
TCGA
RNA-seq
Nomalization
Microarray
2 days ago by
jain72744
▴ 10
6
votes
2
replies
194
views
EdgeR - relationship between logFC and coefficients
differential-expression
r
edger
updated 2 days ago by
Gordon Smyth
★ 7.1k • written 2 days ago by
gBioStar5
▴ 10
2
votes
5
replies
352
views
Importing a fastq file
Fastq
updated 2 days ago by
size_t
▴ 120 • written 3 days ago by
oumo
• 0
3
votes
1
reply
169
views
Question about methylation location
Methylation
WGBS
bisulfide
updated 2 days ago by
dthorbur
★ 1.9k • written 2 days ago by
Eren
• 0
0
votes
0
replies
131
views
Krona Pie-chart taxanomy IDs were not found warning. Taxonomy Classification Metagenomics
Classification
Metagenomics
Krona
Kraken2
2 days ago by
Aytaç
• 0
0
votes
0
replies
144
views
How do I calculate SE or P value if I only have BETA
prscsx
beta
se
pvalue
updated 2 days ago by
zx8754
11k • written 2 days ago by
curious_butterfly
• 0
0
votes
0
replies
137
views
supervised admixture
supervised
admixture
3 days ago by
RT
▴ 10
0
votes
0
replies
139
views
Two-Sample Mendelian Randomization: Association between Instrumental Variable and Outcome
Randomization
Mendelian
two-sample
3 days ago by
Nikki
• 0
2
votes
0
replies
139
views
News:
course on Landscape Genomics at the EPFL in Lausanne (June 17-21)
LandscapeGenomics
SNPs
LocalAdaptation
GIS
3 days ago by
carlopecoraro2
★ 2.5k
0
votes
8
replies
398
views
Different output for read length
samtools
BAM
3 days ago by
marco.barr
▴ 100
116,887 results • Page
1 of 2338
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Answer: joint callset and vcf sorting, unknown TAG issue
Answer: joint callset and vcf sorting, unknown TAG issue
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6.4k
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Recent Replies
Comment: How to remove multiple batch effects from RNA-seq data before limma differential
by
ATpoint
82k
Include confounders into the limma design. No need for upfront removal. Note that libsize is taken care of by normalizing usually. Are you …
Comment: Longest transcript variant per gene
by
sansan_96
▴ 90
Thank you very much, success in your projects.
Comment: In IGV is this a heterogeneous mutation or false call?
by
Tuck898
• 0
Thanks for the input :)
Comment: install package: package ‘gmwm’ is not available for this version of R
by
Pine
▴ 20
This seems to indicate that the R verison you are using (or packages it depends on) are not compatible with the R version you are using any…
Answer: Tissue-specific DEG analysis with DEseq2
by
arctic
▴ 40
Not my area of expertise but GTEx portal seems to offer visual comparison of expression across tissues, maybe checking their pipeline could…
Comment: Is it Possible to "Merge" data from Replicates within a VCF SNP File
by
Pierre Lindenbaum
161k
> I think I am mostly struggling with what would need to be coded to my dataset here and how to run this loop on my set in general. i do…
Comment: Is it Possible to "Merge" data from Replicates within a VCF SNP File
by
ajbarrett98
• 0
Pierre, I am a novice at all of this in general and I have never used Java-based programming before. I may not be able to properly wrangl…
Answer: Practical Haplotype Graph v2 not finding correct paths
by
pjb39
▴ 200
There have been a couple of bugs found that affect imputation accuracy, one in build-kmer-index and one in find-paths. The find-paths bug o…
Comment: Output file of satools flagstat empty
by
Mathew
▴ 100
Hi, In your samtools flagstat, -o specifies the output format. This can be set to 'default', 'json', or 'tsv'. If -o is not specified , t…
Comment: Tissue-specific DEG analysis with DEseq2
by
BioinfGuru
★ 1.7k
I wrote an R package (see [rrdr.io][1], and [github][2]) some years ago to calculate tissue specificity from bulk RNAseq based on [this pap…
Comment: joint callset and vcf sorting, unknown TAG issue
by
Pierre Lindenbaum
161k
> set the Number= to 0 instead of 1; I'm not sure it's a problem. > probably I should also change the Type to Flag ah yes, my bad ! (f…
Comment: Output file of satools flagstat empty
by
GenoMax
142k
If you posted the entire command line then you did not include `-O $MAPPING/${i}.mapping_stats.txt` in that.
Comment: joint callset and vcf sorting, unknown TAG issue
by
Matteo Ungaro
▴ 100
@Pierre Lindenbaum I see. I looked up and, although present, the TAG goes like this in both files: `##INFO=<ID=1000gALT,Number=0,Type=Flag,…
Comment: joint callset and vcf sorting, unknown TAG issue
by
Matteo Ungaro
▴ 100
@Pierre Lindenbaum much appreciated, thanks for the explanation and possible approaches to solve this. I'm not in the position to run `U…
Comment: Tophat multiple or unique mapping criteria
by
Ruqaiya
• 0
> The --b2-mp defines the mismatch penalty: the first number is used when the quality of the base is good, the second when it's bad. If you…
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