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1,000 results • Page
4 of 20
Sort: replies
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Votes
Replies
21
votes
17
replies
9.2k
views
7 follow
Forum:
In YOUR personal opinion, who are the top 5 bioinformatics researchers in NGS in 21st century?
next-gen
updated 13 months ago by
Ram
43k • written 7.4 years ago by
AptRank
▴ 10
26
votes
17
replies
2.3k
views
9 follow
Forum:
GalaxyProject, why and why not?
galaxy
sequencing
pipeline
updated 13 months ago by
Ram
43k • written 8.2 years ago by
support
▴ 50
42
votes
17
replies
38k
views
15 follow
Forum:
List Of Genome Sequencing Centers/Institutes
genomics
institutes
updated 14 months ago by
Ram
43k • written 11.2 years ago by
Malachi Griffith
20k
13
votes
17
replies
4.9k
views
8 follow
Forum:
A Universal/Common Biorep System
reputation
updated 14 months ago by
Ram
43k • written 10.4 years ago by
Obi Griffith
20k
15
votes
17
replies
3.4k
views
10 follow
Forum:
What does a recruiter expect from a Bioinformatician ?
expectations
recruitment
skills
updated 22 months ago by
Ram
43k • written 6.2 years ago by
NB
▴ 960
38
votes
17
replies
3.2k
views
7 follow
Forum:
Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam
bam
samtools
updated 11 months ago by
Ram
43k • written 3.7 years ago by
kojix2
▴ 250
33
votes
17
replies
3.5k
views
7 follow
Forum:
What bioinformatics tools/software do not get enough recognition?
software
updated 14 months ago by
Ram
43k • written 9.5 years ago by
brentp
24k
22
votes
16
replies
7.2k
views
9 follow
Forum:
Large Tables In Supplementary Pdf In Journal Articles
publication
updated 14 months ago by
Ram
43k • written 10.9 years ago by
dario.garvan
▴ 520
21
votes
16
replies
3.2k
views
9 follow
Forum:
Limit Upvotes Over A Certain Amount Of Hours
meta
biostars
updated 15 months ago by
Ram
43k • written 11.3 years ago by
14134125465346445
★ 3.6k
6
votes
16
replies
7.1k
views
6 follow
Forum:
Immune repertoire sequencing community
sequencing
repseq
immunology
tcr
ig
updated 2.2 years ago by
Ram
43k • written 9.8 years ago by
mikhail.shugay
3.5k
51
votes
16
replies
5.9k
views
11 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2011?
papers
updated 15 months ago by
Ram
43k • written 12.4 years ago by
Farhat
★ 2.9k
55
votes
16
replies
6.0k
views
8 follow
Forum:
Mission Impossible: you have 1 minute to analyze the Ebola Genome
ebola
mission-impossible
updated 19 months ago by
Ram
43k • written 9.5 years ago by
Istvan Albert
100k
43
votes
16
replies
4.6k
views
10 follow
Forum:
Need Advice On How To Become A Senior Bioinformatician
advice
career
updated 14 months ago by
Ram
43k • written 11.0 years ago by
calicruz206
▴ 30
61
votes
16
replies
8.1k
views
14 follow
Forum:
Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 16 months ago by
Samuel Lampa
★ 1.3k • written 3.6 years ago by
shenwei356
8.5k
37
votes
16
replies
4.0k
views
9 follow
Forum:
Sanger sequencing is no longer the gold standard?
Sanger
NGS
updated 11 months ago by
Ram
43k • written 6.5 years ago by
lamteva.vera
▴ 220
15
votes
16
replies
1.9k
views
Forum:
Modernising the FASTQ Format
FASTA
Efficiency
FASTQ
updated 13 months ago by
Ram
43k • written 7.2 years ago by
dario.garvan
▴ 520
31
votes
16
replies
2.2k
views
8 follow
Forum:
What is in your opinion the current state-of-the-art sequencing platform for genomic assembly (Nov 2019)
next-gen-sequencing
assembly
genome
updated 11 months ago by
Ram
43k • written 4.5 years ago by
Antonio R. Franco
★ 5.1k
29
votes
16
replies
3.2k
views
Forum:
Making bioinformatics help and tutorials open
ensembl
updated 14 months ago by
Ram
43k • written 9.7 years ago by
Emily
23k
14
votes
16
replies
5.0k
views
8 follow
Forum:
Yet Another Programming Language
Rust
Julia
updated 12 months ago by
Ram
43k • written 6.8 years ago by
Medhat
9.7k
24
votes
16
replies
2.3k
views
6 follow
Forum:
#saveNCBI - it's a joke, right?
ncbi
updated 22 months ago by
Ram
43k • written 8.2 years ago by
5heikki
11k
10
votes
16
replies
2.2k
views
Forum:
Guide for posting better questions
meta
updated 13 months ago by
Ram
43k • written 7.7 years ago by
Medhat
9.7k
18
votes
16
replies
1.8k
views
Forum:
Why are people not working on gene prioritization and candidate gene selection for new experiment design?
gene-prioritization
updated 11 months ago by
Ram
43k • written 5.6 years ago by
prasadhendre
▴ 20
32
votes
16
replies
12k
views
9 follow
Forum:
How to switch from bench research to computational biology?
career
updated 22 months ago by
Ram
43k • written 9.2 years ago by
yuggnurug
▴ 70
30
votes
16
replies
2.3k
views
9 follow
Forum:
Which programming langauge shall I start as a beginner in Bioinformatics
programming-language
single-cell
updated 11 months ago by
Ram
43k • written 4.0 years ago by
nkg.bb
• 0
54
votes
16
replies
5.7k
views
13 follow
Forum:
The new era of bioinformatics: simple and fast tools, fewer and more informative data
kallisto
computational-cost
updated 15 months ago by
Ram
43k • written 9.0 years ago by
Istvan Albert
100k
20
votes
16
replies
3.5k
views
Forum:
Blacklist of questionable genbank entries
genbank
gi
stealth-virus
blacklist
updated 14 months ago by
Ram
43k • written 10.0 years ago by
Michael
54k
23
votes
15
replies
3.7k
views
6 follow
Forum:
Explaining purpose of using FastQC to a colleague
fastqc
updated 13 months ago by
Ram
43k • written 7.3 years ago by
ropolocan
▴ 810
13
votes
15
replies
3.4k
views
8 follow
Forum:
Biostar under spam attack - restrictions added to post content and title
meta
biostars
updated 15 months ago by
Ram
43k • written 8.9 years ago by
Istvan Albert
100k
12
votes
15
replies
2.3k
views
6 follow
Forum:
Opinion: Bioinformatician - What comes first? Biology vs Informatics.
informatics
biology
knowledge
updated 13 months ago by
Ram
43k • written 7.3 years ago by
st.ph.n
★ 2.7k
28
votes
15
replies
4.5k
views
8 follow
Forum:
Anyone Use R For Their Bioinformatics Work?
r
updated 14 months ago by
Ram
43k • written 10.5 years ago by
arronslacey
▴ 320
16
votes
15
replies
6.3k
views
Forum:
Would You Be Interested In Having A Usesthis Like Site For The Bioinformatics Community?
hardware
software
updated 14 months ago by
Ram
43k • written 10.1 years ago by
Drio
▴ 920
22
votes
15
replies
9.0k
views
11 follow
Forum:
RNASeq user experience with Novogene?
RNA-Seq
updated 11 months ago by
Ram
43k • written 5.9 years ago by
ivanarg2
▴ 80
19
votes
15
replies
2.0k
views
6 follow
Forum:
Cross out line in Biostars
meta
updated 11 months ago by
Ram
43k • written 6.0 years ago by
Bastien Hervé
5.3k
6
votes
15
replies
1.7k
views
Forum:
PhD Bioinformatics on GWAS
SNP
GWAS
genome
PHD
updated 11 months ago by
Ram
43k • written 4.3 years ago by
davidenoma
▴ 50
23
votes
15
replies
5.1k
views
6 follow
Forum:
On the usage of Golang
assembly
next-gen
alignment
updated 11 months ago by
Ram
43k • written 5.6 years ago by
Lucas Peres
▴ 80
57
votes
15
replies
6.3k
views
9 follow
Forum:
Good practices while dealing with very large datasets
Big-Data
Programming
updated 12 months ago by
Ram
43k • written 7.0 years ago by
VHahaut
★ 1.2k
8
votes
15
replies
2.4k
views
7 follow
Forum:
How to identify your analytic data is true?
RNA-Seq
ChIP-Seq
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.8 years ago by
Hughie
▴ 80
20
votes
15
replies
6.8k
views
Forum:
How To Analyze Your Own Exome
exome
filtering
analysis
vcf
variant
updated 3.1 years ago by
Ram
43k • written 10.7 years ago by
alexej.knaus
▴ 130
21
votes
15
replies
2.1k
views
9 follow
Forum:
Working abroad without a PhD
career
updated 11 months ago by
Ram
43k • written 4.6 years ago by
Rox
★ 1.4k
24
votes
15
replies
2.0k
views
Forum:
Users of 0 rep required to read etiquette guides before first post?
meta
updated 13 months ago by
Ram
43k • written 8.2 years ago by
John
13k
13
votes
15
replies
2.2k
views
Forum:
Posting questions on Biostars without Googling or a preliminary Literature search
meta
biostars
updated 13 months ago by
Ram
43k • written 7.5 years ago by
Rohit
★ 1.5k
24
votes
15
replies
11k
views
11 follow
Forum:
PhD in bioinformatics or biostatistics as a wet-lab scientist?
biology
statistics
PhD
updated 14 months ago by
Ram
43k • written 9.7 years ago by
benjamin.hebisch
▴ 70
10
votes
15
replies
2.1k
views
Forum:
How should reference genome fasta files be distributed by UCSC?
reference-genome
fasta
freeze
ucsc-genome-browser
updated 11 months ago by
Jeremy Leipzig
22k • written 14 months ago by
Maximilian Haeussler
★ 1.6k
23
votes
15
replies
2.5k
views
10 follow
Forum:
I'm in a Masters of Bioinformatics Program and wanted to ask everyone for advice
career
updated 13 months ago by
Ram
43k • written 8.1 years ago by
seekheart2009
▴ 20
42
votes
15
replies
7.8k
views
8 follow
Forum:
Directions In Bioinformatics And Web-Based Data Visualization: Revisiting Javascript?
data-visualization
python
r
updated 14 months ago by
Ram
43k • written 11.5 years ago by
Josh Herr
5.8k
8
votes
15
replies
4.1k
views
Forum:
Can Galaxy Be Used Under The Hood? How About The Galaxy Appliance?
galaxy
updated 14 months ago by
Ram
43k • written 10.8 years ago by
seidel
11k
57
votes
15
replies
4.8k
views
9 follow
Forum:
Entice Biologists To Learn R
r
updated 15 months ago by
Ram
43k • written 12.6 years ago by
Madelaine Gogol
5.3k
28
votes
15
replies
2.3k
views
12 follow
Forum:
Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 11 months ago by
Ram
43k • written 5.8 years ago by
ivan.radonov
▴ 10
7
votes
15
replies
2.9k
views
Forum:
Masters in Bioinformatics
ChIP-Seq
genome
next-gen
R
RNA-Seq
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
gs.shagy
• 0
19
votes
14
replies
2.2k
views
Forum:
What is the amount of sequencing data produced annually?
data
research
sequencing
updated 7 months ago by
Jeremy Leipzig
22k • written 7 months ago by
vincenthus
▴ 70
1,000 results • Page
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Recent Votes
Comment: Question about methylation location
Comment: Question about methylation location
Comment: Question about methylation location
How to use minimap2?
Comment: RNA seq analysis
Comment: Filtering Multi-sample VCF file for all except one Genotype
Truncated metadata file report from ENA Portal API
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Comment: cellranger error message
by
Max
• 0
I think the problem is your fastq_id. This should also read "RabhiN_PHLGEX". Hope this helps!
Comment: How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
by
Giulio Genovese
▴ 390
@yokofakun is correct. Also, do notice that the GATK option `--RECOVER_SWAPPED_REF_ALT True` does not work with indels. In general, if your…
Comment: How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
by
Pierre Lindenbaum
161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: How to format "I" and "D" in vcf version 4.2 for liftover analysis in GATK
by
Pierre Lindenbaum
161k
I think this is useless, in the gatk doc: > For each variant, the tool will look for the target coordinate, reverse-complement and left-al…
Comment: Too many unpaired forward reads found by Trimmomatic
by
GenoMax
142k
Ideally you will know which adapters to use but if you don't then programs like `fastp` can auto-detect them. You could also use `bbduk.sh`…
Comment: UMI-Tools knee-method has great influence on the results of white list
by
i.sudbery
19k
With the `--read2-only` option (which isn't available yet on the bioconda version), this will complain about a lack of `--bc-pattern`. How…
Comment: samtools tview symbols
by
Ruqaiya
• 0
What does the star mean then ?
Comment: RNA seq analysis
by
prifa
▴ 10
it's raw data **fastq.gz**, so for example I have 4 different samples from different tissue, sample A with 3 replicates, samples B,C and D …
Answer: Filtering Multi-sample VCF file for all except one Genotype
by
Pierre Lindenbaum
161k
> The goal is to remove all the variants which are unique to the KWSBambina sample. using jvarkit vcffilterjdk https://jvarkit.readthedo…
Comment: Too many unpaired forward reads found by Trimmomatic
by
SilhouetteQ
• 0
The first example used the adapter file NexteraPE-PE.fa, but it generated more than 50% unpaired data...... The content of NexteraPE-PE.fa…
Comment: Filtering Multi-sample VCF file for all except one Genotype
by
schmince
• 0
Ok so I definitely didn't understand at first what my goal was. The goal is to remove all the variants which are unique to the KWSBambina…
Comment: Question about methylation location
by
dthorbur
★ 1.9k
What data do you have? Generally, you start with sequencing data, which you need to clean and then map to a reference genome. Then dependin…
Comment: Too many unpaired forward reads found by Trimmomatic
by
GenoMax
142k
> when I didn't provide the adapter file Still see the file here `ILLUMINACLIP:./NexteraPE-PE.fa:2:30:10 `, unless that is an empty file.
Comment: Too many unpaired forward reads found by Trimmomatic
by
SilhouetteQ
• 0
Interestingly, when I didn't provide the adapter file, the number of unpaired forward reads was significantly reduced: TrimmomaticPE…
Comment: Alignment of samples with spike-in
by
GenoMax
142k
> but then if you only allow to map uniquely mapped reads, you are losing spike-in information, How so? Spike-in's are generally unique s…
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