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460 results • Page
3 of 10
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Views
Votes
Replies
36
votes
34
replies
2.8k
views
9 follow
Forum:
New to bioinformatics.....
gene
updated 11 months ago by
Ram
43k • written 6.1 years ago by
sms.00196
• 0
3
votes
4
replies
2.8k
views
Forum:
hands on resources for high school students with advanced CS skills
informatics
education
high-school
updated 11 months ago by
Ram
43k • written 5.8 years ago by
LauferVA
4.2k
1
vote
13
replies
2.8k
views
Forum:
Bioinformatics computer upgrade help
computer
updated 10 months ago by
Ram
43k • written 3.8 years ago by
truebeliever24
▴ 50
2
votes
3
replies
2.8k
views
Forum:
What is the most popular programming language used in bioinformatics?
programming-languages
updated 11 months ago by
Ram
43k • written 6.9 years ago by
inkprs
▴ 70
7
votes
6
replies
2.8k
views
7 follow
Forum:
Should I specialise in bioinformatics?
career
updated 11 months ago by
Ram
43k • written 5.5 years ago by
saulpierotti
▴ 10
31
votes
41
replies
2.7k
views
12 follow
Forum:
Question regarding journal publications
publications
journals
updated 10 months ago by
Ram
43k • written 4.2 years ago by
K.Gee
▴ 40
8
votes
4
replies
2.7k
views
Forum:
SNP calling in 2018
variant
gatk
SNP
updated 11 months ago by
Ram
43k • written 6.2 years ago by
Picasa
▴ 640
34
votes
8
replies
2.7k
views
9 follow
Forum:
Bioinformatician, a jack of all trades, but in the eye of the CV-beholder a master of none? How can your CV reflect the diverse skills that you have …
cv
industry
career
updated 10 months ago by
Ram
43k • written 4.5 years ago by
'
▴ 300
29
votes
18
replies
2.7k
views
12 follow
Forum:
what are the big questions in bioinformatics field nowadays
genome
updated 10 months ago by
Ram
43k • written 4.2 years ago by
t4192
▴ 20
12
votes
5
replies
2.6k
views
Forum:
We Need Your Input! Help Get Information For The Informatics Community And Get A Free Gift Card!
algorithm
ngs
updated 10 months ago by
Ram
43k • written 10.9 years ago by
BioPlanet
▴ 30
2
votes
2
replies
2.6k
views
Forum:
What are the challenges faced in bioinformatics research
sequencing
alignment
updated 11 months ago by
Ram
43k • written 6.9 years ago by
Sundeep Kumar
▴ 10
15
votes
24
replies
2.6k
views
7 follow
Forum:
Airport.bio: First class ticket to any biological database in the world
RNA-Seq
ChIP-Seq
SNP
updated 11 months ago by
Ram
43k • written 6.1 years ago by
Bohdan Khomtchouk
▴ 350
0
votes
4
replies
2.6k
views
Forum:
Quantum Computing in Genomics
genome
next-gen
RNA-Seq
updated 11 months ago by
Ram
43k • written 6.2 years ago by
hafiz.talhamalik
▴ 350
3
votes
4
replies
2.6k
views
Forum:
How long does it take to build a pipeline?
next-gen
self-learning
updated 11 months ago by
Ram
43k • written 6.6 years ago by
lamteva.vera
▴ 220
3
votes
27
replies
2.6k
views
7 follow
Forum:
What server do you use?
HPC
AWS
updated 11 months ago by
Ram
43k • written 5.8 years ago by
caggtaagtat
★ 1.9k
2
votes
3
replies
2.5k
views
Forum:
R books with Quantitative Genetics examples?
R
updated 11 months ago by
Ram
43k • written 6.2 years ago by
thegreatelector
• 0
14
votes
6
replies
2.5k
views
6 follow
Forum:
Bioinformatics experience in programming
python
R
updated 11 months ago by
Ram
43k • written 5.7 years ago by
lucas_
▴ 20
21
votes
11
replies
2.5k
views
6 follow
Forum:
Rust bioinformatics projects ideas
projects
scRNA-seq
RNA-seq
Rust
updated 5 months ago by
Jeremy Leipzig
22k • written 14 months ago by
bompipi95
▴ 150
43
votes
37
replies
2.5k
views
7 follow
Forum:
Checking the quality and reliability in bioinformatics
reproducibility
updated 11 months ago by
Ram
43k • written 6.6 years ago by
zizigolu
★ 4.3k
18
votes
12
replies
2.5k
views
6 follow
Forum:
why GATK makes things more complicated ?
genomics
updated 11 months ago by
Ram
43k • written 5.2 years ago by
Learner
▴ 280
6
votes
6
replies
2.5k
views
Forum:
Trouble finding job with MS in Bioinformatics
next-gen
updated 11 months ago by
Ram
43k • written 5.9 years ago by
Moneeb Bajwa
▴ 10
6
votes
4
replies
2.5k
views
Forum:
Ru/Read Until in Nanopore sequencers
Oxford-Nanopore
alignment
sequencing
updated 10 months ago by
Ram
43k • written 4.2 years ago by
jean.elbers
★ 1.7k
8
votes
6
replies
2.4k
views
Forum:
How do you organize sequence data (FASTQs)?
RNA-Seq
sequencing
updated 11 months ago by
Ram
43k • written 6.0 years ago by
Daniel E Cook
▴ 280
11
votes
9
replies
2.4k
views
7 follow
Forum:
Feature Selection and Dimensionality Reduction in 10X scRNA-seq data
software-error
pca
scRNA-seq
updated 11 months ago by
Ram
43k • written 4.3 years ago by
ATpoint
82k
12
votes
14
replies
2.4k
views
8 follow
Forum:
Recovering bam files after unknown deletion in the storage
photorec
bam
sequencing
written 4.9 years ago by
Gabriel Wajnberg
▴ 70
8
votes
15
replies
2.4k
views
7 follow
Forum:
How to identify your analytic data is true?
RNA-Seq
ChIP-Seq
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.7 years ago by
Hughie
▴ 80
3
votes
1
reply
2.4k
views
Forum:
Pipeline suggestions for GO & pathway analysis for species without reference genome
next-gen
RNA-Seq
de-novo
updated 11 months ago by
Ram
43k • written 6.9 years ago by
Whoknows
▴ 960
18
votes
11
replies
2.4k
views
8 follow
Forum:
Create a File format to store Variant
hgvs
json
variant
updated 11 months ago by
Ram
43k • written 5.8 years ago by
sacha
★ 2.4k
5
votes
5
replies
2.4k
views
Forum:
BCL files as deliverables from a sequencing center
core-facility
service-provider
updated 11 months ago by
Ram
43k • written 4.9 years ago by
Ido Tamir
5.2k
9
votes
14
replies
2.4k
views
Forum:
MS Bioinformatics Project
assembly
sequencing
alignment
R
SNP
updated 11 months ago by
Ram
43k • written 6.1 years ago by
Moneeb Bajwa
▴ 10
7
votes
8
replies
2.4k
views
Forum:
Virus Genome Length and GC Content of SARS-COV-2 causing COVID-19
sequence
genome
updated 10 months ago by
Ram
43k • written 4.1 years ago by
dulankak
▴ 30
15
votes
10
replies
2.3k
views
6 follow
Forum:
help identifying bioinformatics operations tools
database
next-gen
operations
genomics
updated 11 months ago by
Ram
43k • written 5.4 years ago by
jsmedmar
▴ 80
11
votes
9
replies
2.3k
views
Forum:
Survey: help define Gencode and NCBI primary transcripts
refseq
ensembl
gencode
ncbi
transcript
updated 11 months ago by
Ram
43k • written 6.1 years ago by
Emily
23k
34
votes
5
replies
2.3k
views
Forum:
what we should reply when we are asked do you have any question?
career
interview
updated 11 months ago by
Ram
43k • written 6.9 years ago by
zizigolu
★ 4.3k
6
votes
5
replies
2.3k
views
Forum:
Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by
tshtatland
▴ 190
6
votes
1
reply
2.3k
views
Forum:
How to become employable in Bioinformatics, starting with degree and experience in CS/Software Engineering?
computer-scientist
software-engineer
updated 11 months ago by
Ram
43k • written 5.7 years ago by
Fanta
• 0
28
votes
15
replies
2.2k
views
12 follow
Forum:
Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 11 months ago by
Ram
43k • written 5.8 years ago by
ivan.radonov
▴ 10
30
votes
16
replies
2.2k
views
9 follow
Forum:
Which programming langauge shall I start as a beginner in Bioinformatics
programming-language
single-cell
updated 10 months ago by
Ram
43k • written 4.0 years ago by
nkg.bb
• 0
0
votes
1
reply
2.2k
views
Forum:
How's the bioinformatics engineering path? How to become a bioinformatics engineer?
machine-learning
Bioinformatics-engineer
updated 10 months ago by
Ram
43k • written 4.0 years ago by
SupBioInformatics
▴ 60
4
votes
2
replies
2.2k
views
Forum:
wet-lab vs dry-lab scientists
cv
language
updated 10 months ago by
Ram
43k • written 4.2 years ago by
igor
13k
10
votes
7
replies
2.2k
views
Forum:
How to learn bioinformatics?
learning
updated 3 months ago by
Samuel Lampa
★ 1.3k • written 23 months ago by
sakichwan
▴ 20
10
votes
6
replies
2.2k
views
Forum:
How to acknowledge in the paper a reference genome that hasn't been published yet?
reference
paper
updated 11 months ago by
Ram
43k • written 5.7 years ago by
ciemanek
▴ 140
31
votes
16
replies
2.2k
views
8 follow
Forum:
What is in your opinion the current state-of-the-art sequencing platform for genomic assembly (Nov 2019)
next-gen-sequencing
assembly
genome
updated 10 months ago by
Ram
43k • written 4.5 years ago by
Antonio R. Franco
★ 5.1k
0
votes
4
replies
2.2k
views
Forum:
Multiple Sequence Alignment Applications
snp
updated 11 months ago by
Ram
43k • written 4.8 years ago by
manimphil89
• 0
15
votes
9
replies
2.2k
views
6 follow
Forum:
[Opinion] - What alignment software do you use?
next-gen
alignment
updated 11 months ago by
Ram
43k • written 6.1 years ago by
Andrew_MacGregor
▴ 30
7
votes
4
replies
2.2k
views
Forum:
How hard it is to get a bioinformatics degree in one country but find a career in another country?
career
Masters
updated 11 months ago by
Ram
43k • written 5.9 years ago by
dominic.dong
▴ 10
1
vote
6
replies
2.1k
views
Forum:
shotgun sequencing and clone-based sequencing
sanger-sequencing
updated 11 months ago by
Ram
43k • written 6.6 years ago by
mm521
• 0
14
votes
10
replies
2.1k
views
Forum:
WGBS alignment with BitMapperBS
BitMapperBS
bismark
updated 11 months ago by
Ram
43k • written 4.8 years ago by
Shicheng Guo
★ 9.4k
3
votes
6
replies
2.1k
views
Forum:
Choosing the appropriate test for differential analysis: Limma or Mann-Whitney (with BH correction)? Test for normality
R
Shapiro-Wilk
Limma
Mann-Whitney
updated 10 months ago by
Ram
43k • written 4.0 years ago by
innyus
▴ 40
24
votes
26
replies
2.1k
views
6 follow
Forum:
Organizing a Bioinformatics Conference ?
meeting
conference
updated 11 months ago by
Ram
43k • written 6.5 years ago by
Pierre Lindenbaum
161k
460 results • Page
3 of 10
Recent Votes
Comment: NGS forensics: how to know if data is fabricated
Comment: How many reads for WGS Sequencing?
Comment: How many reads for WGS Sequencing?
Comment: How many reads for WGS Sequencing?
Comment: How many reads for WGS Sequencing?
Comment: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
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Recent Replies
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 540
Working on it - it's actually a bit difficult. Low coverage of ribosomal proteins. Low coverage of housekeeping genes ...I'm trying to find…
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 540
I'm a bit against pubpeer - have you ever posted there? My gripe with them is that the posts are heavily moderated and EDITED to the point…
Comment: NGS forensics: how to know if data is fabricated
by
Philipp Bayer
8.4k
Thanks Mensur! I'd add that in 9 out of 10 cases, you won't receive a reply to a request for raw data. That's just how scientists are, does…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
I had downloaded the two files in a similar manner actually.
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
ok, i'll try to use fastp. I have only used trimmomatic and cutadapt till now and they dont identify on its own. Except the graph in fastqc…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
got it! even if i'm doing the assembly for paired end with both the files do i have set the threshold to 1000bp ?
Comment: NGS forensics: how to know if data is fabricated
by
Philipp Bayer
8.4k
i've been in our org trying to push such a detector, our use-case is much more limited: I want to make simulated eDNA libraries as real as …
Answer: Differential Expression using Isoseq-supplemented reference transcriptome
by
Gordon Smyth
★ 7.0k
> What is the best method to test for differential abundance at the isoform level? Salmon returns TPM values instead of the counts which Li…
Comment: NGS forensics: how to know if data is fabricated
by
benformatics
3.9k
Can you post an IGV screenshot of what this looks like?
Comment: NGS forensics: how to know if data is fabricated
by
benformatics
3.9k
Yeah some of the Illumina machines pre-trim adapters (not sure if this is still the standard). I've also definitely also seen quality-trimm…
Comment: PDB related issue
by
noodle
▴ 540
You can typically find much more useful info in the cif file, for example what you've found is called '_atom_site.pdbx_PDB_ins_code ' and d…
Comment: PDB related issue
by
Mensur Dlakic
★ 27k
The best way to find out is to read the original paper. My guess is that the residue has been crystallized in alternative conformations, wh…
Answer: NGS forensics: how to know if data is fabricated
by
Mensur Dlakic
★ 27k
I share the opinion by @philippbayer I have seen enough questionable things in life and in science to keep me permanently jaded, but there…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
I downloaded the data in two files from the link you provided. With this command: megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
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