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778 results • Page
4 of 16
Sort: Views
Rank
Views
Votes
Replies
0
votes
3
replies
853
views
Mapping fastq files to reference genome
reference-genome
mapping
fastq
updated 4 days ago by
Ram
40k • written 2.3 years ago by
A_heath
▴ 140
4
votes
12
replies
845
views
bedops alternative for bedtools -unionbedg ?
bedtools
bam
coverage
bedops
bed
updated 10 days ago by
Alex Reynolds
35k • written 26 days ago by
Agastya
▴ 10
2
votes
11
replies
843
views
Extracting regions around sites in VCF file and writing them as fasta sequences/files
VCF
fasta
updated 13 days ago by
GenoMax
134k • written 27 days ago by
Aiswarya
▴ 10
8
votes
9
replies
839
views
What does a gene's "aligned length" represent?
alignment
25 days ago by
Ethan Lee
• 0
5
votes
12
replies
824
views
Converting custom VCF to standard VCF
VCF
11 days ago by
avelarbio46
▴ 30
1
vote
3
replies
817
views
using scanpy to merge objects
scanpy
scRNA-seq
single-cell
updated 11 days ago by
yl759
▴ 40 • written 3 months ago by
Andy
▴ 90
2
votes
4
replies
804
views
gosling.js and React 1.7+
react
gosling
updated 3 days ago by
Ram
40k • written 13 months ago by
Bin
▴ 10
0
votes
1
reply
802
views
Fossil calibration in PAML software?
Evolution
updated 24 days ago by
Lada
▴ 30 • written 4.2 years ago by
860101959
▴ 10
8
votes
4
replies
786
views
BLASTn: extract unmatched regions only
BLASTn
updated 4 days ago by
Ram
40k • written 22 months ago by
A_heath
▴ 140
0
votes
7
replies
786
views
Mugsy error -directory must be a directory
Mugsy
updated 4 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
11
votes
8
replies
761
views
6 follow
Forum:
How to write a review article in bioinformatics
article
review
updated 25 days ago by
Ram
40k • written 6 weeks ago by
joey
▴ 10
0
votes
2
replies
761
views
How to decrypt EGA .crypt files?
EGA
updated 16 days ago by
vinayjrao
▴ 220 • written 21 months ago by
Sammy
▴ 20
0
votes
1
reply
756
views
How to identify specific region using Mauve?
Mauve
primers
updated 4 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
6
votes
4
replies
751
views
How to rename file titles that contain underscores?
shell
bash
updated 4 days ago by
Ram
40k • written 3.1 years ago by
A_heath
▴ 140
4
votes
5
replies
750
views
Getting Intron Positions from Amino Acid Sequences
acid
positions
amino
sequences
intron
17 days ago by
fafad046
• 0
1
vote
11
replies
746
views
Quantification after transcriptome assembly with Trinity
De-novo-transcriptome-assembly
Trinity
RNA-seq-analysis
updated 1 day ago by
GenoMax
134k • written 28 days ago by
langziv
▴ 50
2
votes
2
replies
746
views
How to calculate foldchange for log2'd expression data?
fold-change
t-test
updated 10 days ago by
Ram
40k • written 3.0 years ago by
SupBioInformatics
▴ 60
4
votes
9
replies
745
views
Which minimap2 parameters to set while performing mapping to avoid such mismatches
minimap2
23 days ago by
Mo
▴ 40
5
votes
4
replies
738
views
Primer designing for full length gene amplification
gene-amplification
primer-design
offtopic
updated 6 days ago by
Ram
40k • written 16 months ago by
Fizzah
▴ 30
0
votes
7
replies
737
views
Converting string to numerical in bcftools
bcftools
updated 28 days ago by
AMARU
• 0 • written 10 weeks ago by
pjuge
• 0
1
vote
1
reply
729
views
GO enrichment and KEGG output empty
enrichment
GO
KEGG
clusterprofiler
NCBI
updated 27 days ago by
cardon.chc
• 0 • written 24 months ago by
estpallag
▴ 10
0
votes
3
replies
727
views
How to add translation of CDS in Artemis?
translation
Artemis
updated 4 days ago by
Ram
40k • written 2.7 years ago by
A_heath
▴ 140
0
votes
11
replies
725
views
Normalization for microarrays >1000+ samples?
microarray
normalization
oligo
5 days ago by
evmae
• 0
4
votes
7
replies
724
views
How to subtract a number from protein IDs?
blast
updated 4 days ago by
Ram
40k • written 3.1 years ago by
A_heath
▴ 140
6
votes
10
replies
720
views
Making pairwise matrix using ChIP-Seq peak binding matrix in R
R
matrix
binding
Chip-seq
20 days ago by
Ankit
▴ 390
1
vote
2
replies
719
views
Job:
Scientist Position, Data Science AI/ML for Biology (JRD DS)
Data-Science
JNJ
Janssen
updated 11 hours ago by
Ram
40k • written 13 months ago by
Shicheng Guo
★ 9.3k
7
votes
3
replies
713
views
blastn with short sequences gives no results in the corresponding genome
primers
blastn
updated 4 days ago by
Ram
40k • written 2.7 years ago by
A_heath
▴ 140
8
votes
10
replies
713
views
Inflated GWAS test statistic after merging multiple batches of genotype dataset imputed differently
genotype
imputation
gwas
updated 5 days ago by
LauferVA
3.7k • written 6 days ago by
Amy
▴ 20
4
votes
8
replies
711
views
SRA-toolkit installation: "fastq-dump not found"
fastq
SRA-toolkit
updated 17 days ago by
river
• 0 • written 12 weeks ago by
BioGuy
• 0
7
votes
13
replies
711
views
Help with Error: Required version of NumPy not available: installation of Numpy >= 1.6 not found
seurat
leiden
25 days ago by
Chris
▴ 180
0
votes
4
replies
706
views
Is there a way to get a list of all homogygous sites for each sample with plink (regardless if they are in a run)
plink
updated 1 day ago by
chrchang523
10k • written 16 days ago by
curious
▴ 730
3
votes
6
replies
705
views
6 follow
Forum:
Is Bioinformatics a Professional Degree?
career
degree
26 days ago by
anasjamshed
▴ 120
0
votes
1
reply
696
views
Is there a way to subsample Hi-C at Hi-C matrix .cool file?
Hi-C
loops
updated 21 days ago by
kalavattam
▴ 180 • written 2.3 years ago by
sckinta
▴ 730
0
votes
0
replies
689
views
Is it possible to combine count gene expression data coming from one sequencing technology (RNA seq) with intensity gene expression data coming from …
Differential-expression
Normalization
updated 25 days ago by
Ram
40k • written 25 days ago by
Sep
• 0
0
votes
0
replies
685
views
Job:
Research Scientist 5 – Bioinformatics and Computer Biology, Center for Dementia Research (CDR), Nathan Kline Institute for Psychiatric Research
Neuroscience
Biostatistics
updated 5 days ago by
Ram
40k • written 5 days ago by
cdrinfo
• 0
0
votes
2
replies
685
views
pal2nal -nogap -nomismatch options not working
pal2nal
updated 10 days ago by
wangjb702
• 0 • written 10 months ago by
pkfsantos
• 0
2
votes
7
replies
681
views
VG mapping paired-end reads: error [xg]: multiple hits for XXX
variation-graph
reads
pangenome
vg
paired-end
22 days ago by
nkls063408
• 0
1
vote
2
replies
680
views
What is an appropriate cut off for p-values and log fold changes during the analysis of a time course global gene expression experiment using DESeq2?
LFC
Deseq2
p-value
updated 28 days ago by
kalavattam
▴ 180 • written 4 weeks ago by
mgranada3
▴ 20
6
votes
5
replies
679
views
ChIP-seq visualization: Is it valid to do a coverage normalization in addition to applying a spike-in-derived scaling factor?
ChIP-seq
normalization
scaling-factor
coverage
spike-in
updated 20 days ago by
Ram
40k • written 26 days ago by
kalavattam
▴ 180
0
votes
2
replies
677
views
How do I use PLINK to filter out SNPs?
PLINK
r
SNPS
updated 19 days ago by
karthivalarmathi99
▴ 10 • written 19 months ago by
melissachua90
▴ 40
1
vote
4
replies
677
views
Tumor-infiltrating immune cells
cell
TIMER
immune
Tumor-infiltrating
TPM-normalized
updated 11 days ago by
Ram
40k • written 14 months ago by
J.farideh76
• 0
1
vote
3
replies
672
views
How to determine plasmid or chromosomal origin?
plasmid
chromosomal-origin
updated 4 days ago by
Ram
40k • written 3.0 years ago by
A_heath
▴ 140
0
votes
7
replies
668
views
build error with vg tools
ubuntu
vg
vgtools
updated 22 days ago by
z911741
• 0 • written 11 weeks ago by
rimo
▴ 10
1
vote
5
replies
667
views
Mugsy percentage of identity
Mugsy
Genome-alignment
updated 4 days ago by
Ram
40k • written 2.5 years ago by
A_heath
▴ 140
3
votes
2
replies
661
views
How to download Time Calibration Data for Phylogenetic tree estimation ?
gene
DNA
RNA
updated 24 days ago by
Lada
▴ 30 • written 2.4 years ago by
sunnykevin97
▴ 970
2
votes
9
replies
657
views
Forum:
open source projects looking for contributors
open-source
updated 13 days ago by
vincenthus
▴ 70 • written 27 days ago by
claudiofr
▴ 20
0
votes
1
reply
655
views
How to get enrichment of ERVs in differentially expressed peaks from ATAC-seq data?
ChIP-Seq
ERVs
ATAC-seq
updated 10 days ago by
pb11
▴ 10 • written 2.8 years ago by
fdemiguelsdp
• 0
0
votes
13
replies
651
views
Dealing with transcriptome sequences that are smaller than their respective genes
blastn
Trinity
Transcriptome
RNA-seq-analysis
De-novo-transcriptome-assembly
3 days ago by
langziv
▴ 50
5
votes
9
replies
649
views
RNA-seq for differential gene expression analysis with only one biological replicates
RNA-seq
11 days ago by
WMA
• 0
1
vote
3
replies
646
views
What are the definitions of the BBMerge outinsert table file?
bbmerge
outinsert
updated 28 days ago by
Dave Carlson
★ 1.4k • written 2.9 years ago by
Tawny
▴ 180
778 results • Page
4 of 16
Recent Votes
Comment: Quantification after transcriptome assembly with Trinity
Snakemake: output folder a as dependency to a different rule
A: Snakemake: output folder a as dependency to a different rule
A: In Seurat, How Do nCount_RNA Differ from nFeature_RNA?
How to identify 16s sequences from binning data(contigs)?
geom_signif() uses t-test to compare between more than 3 groups... Isn't this wrong?
Answer: Filter transcription factors
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Recent Replies
Answer: Gene enrichment analysis of prokaryotes gene
by
dthorbur
▴ 550
[Here](https://github.com/golden75/prokaryote_RNASeq) is a tutorial on how to use the R package DESeq2 for prokaryotic RNAseq analyses. [H…
Comment: Read block operation failed with BAM file
by
John Marshall
3.0k
Indeed, though it is a seek issue when accessing files over HTTP and other network protocols. So, unless there's something you didn't menti…
Comment: geom_signif() uses t-test to compare between more than 3 groups... Isn't this wr
by
RM123
▴ 10
Thank you for your help. I am comparing 4 groups, but as I have gene expression data I have a considerable number of boxplots to make. I fo…
Comment: gene correlations in between two groups
by
ATpoint
76k
And how should I know this? I cannot read minds, nor see your screen, data or anything. Please ask a good question with necessary details. …
Comment: gene correlations in between two groups
by
edus_bioinfo
▴ 40
Thanks. But they are already analyzed. Afaik it analyzes raw data. In my dataset for each gene there is score. I want to interpret the data…
Answer: GWAS phenotype
by
dthorbur
▴ 550
Hello again. Yes, you can conduct GWAS using binary trait data, but it comes with a whole suite of complications. See [this](https://ww…
Comment: geom_signif() uses t-test to compare between more than 3 groups... Isn't this wr
by
RM123
▴ 10
Hello Dariober, That seems like a good solution, however I'm pretty new to R. Could you tell me how I can do that?
Comment: how to create a loop in R
by
Nicolas Rosewick
10k
Not really a bioinformatics related question. Start by looking at R tutorial, there is plenty of them online e.g. : https://www.statmethods…
Comment: Violin plot (Monocle 3) - Troubleshooting
by
fracarb8
★ 1.2k
You did not add the screenshot.
Answer: BED files
by
Alex Reynolds
35k
Generically via `bedmap`: ``` bedmap --echo --echo-map --count exome.bed annotations.bed > answer.bed ``` The `annotations.bed` fil…
Comment: geom_signif() uses t-test to compare between more than 3 groups... Isn't this wr
by
dariober
14k
It seems to me that anova followed by HSD is a more sensible approach than applying independent t-tests. Granted this is the first time I s…
Comment: BED files
by
barslmn
★ 1.8k
I am guessing you want the annotation files. https://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/annotation/GRCh38_latest/refseq_identifiers/
Comment: Filter human transcription factors
by
Alex Reynolds
35k
Curious how redundant these TFs are. Jeff Vierstra has done some analysis on this to simplify model sets: https://www.vierstra.org/resource…
Comment: Read block operation failed with BAM file
by
Alex Reynolds
35k
https://github.com/samtools/htslib/pull/1676 patches a seek issue in `htslib` (upon which `samtools` and `pysam` depend)
Comment: Merging the filename with tsv files for master file
by
barslmn
★ 1.8k
Could you add samples from your files and your expected output.
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