updated 5 hours ago by
Akash D
▴
50
•
written 6.5 years ago by
rob.costa1234
▴
310
7
votes
3
replies
13k
views
22
votes
15
replies
10k
views
12 follow
updated 8 hours ago by
Thanujay S
•
0
•
written 12.5 years ago by
woemler
▴
170
9
votes
9
replies
6.5k
views
6 follow
3
votes
4
replies
6.3k
views
updated 12 hours ago by
pramach1
▴
40
•
written 9.8 years ago by
tptacek3050
▴
70
5
votes
4
replies
5.1k
views
2
votes
4
replies
1.1k
views
2 hours ago by
Aspire
▴
330
1
vote
3
replies
902
views
updated 11 hours ago by
David-walson
•
0
•
written 16 months ago by
Apprentice
▴
160
1
vote
8
replies
834
views
updated 16 hours ago by
a.penatauber
•
0
•
written 3 months ago by
Lucas R.F.
▴
10
1
vote
4
replies
384
views
3
votes
6
replies
324
views
updated 18 hours ago by
Ram
44k
•
written 1 day ago by
anasjamshed
▴
120
2
votes
6
replies
258
views
7 hours ago by
anikcropscience
▴
230
2
votes
4
replies
250
views
17 hours ago by
Arton
▴
10
0
votes
6
replies
247
views
updated 16 hours ago by
ATpoint
82k
•
written 1 day ago by
machaalani29
•
0
1
vote
6
replies
243
views
updated 3 hours ago by
aw7
▴
310
•
written 1 day ago by
LucisTheFather
•
0
0
votes
1
reply
219
views
updated 4 hours ago by
韩雨
•
0
•
written 5 weeks ago by
solomoncharles77
▴
90
1
vote
3
replies
201
views
0
votes
4
replies
181
views
16 hours ago by
reza
▴
300
0
votes
2
replies
179
views
5
votes
3
replies
170
views
updated 22 hours ago by
GenoMax
142k
•
written 22 hours ago by
Christopher
▴
10
1
vote
2
replies
169
views
19 hours ago by
Tuấn Anh
•
0
0
votes
3
replies
153
views
updated 15 hours ago by
GenoMax
142k
•
written 16 hours ago by
Shicheng Guo
★
9.4k
1
vote
1
reply
151
views
0
votes
1
reply
141
views
updated 18 hours ago by
Ram
44k
•
written 1 day ago by
He11oJe11o
•
0
0
votes
1
reply
113
views
1
vote
1
reply
113
views
updated 12 hours ago by
Jeremy
▴
910
•
written 14 hours ago by
Mohamed Samir
▴
30
0
votes
1
reply
110
views
0
votes
0
replies
108
views
0
votes
0
replies
96
views
0
votes
1
reply
95
views
updated 16 hours ago by
Pierre Lindenbaum
162k
•
written 17 hours ago by
avelarbio46
▴
30
1
vote
2
replies
84
views
0
votes
0
replies
85
views
updated 18 hours ago by
Ram
44k
•
written 23 hours ago by
letizia.ottaviani
•
0
1
vote
4
replies
80
views
50 minutes ago by
NTerway
•
0
0
votes
0
replies
82
views
8 hours ago by
Tim
•
0
0
votes
0
replies
75
views
1
vote
2
replies
69
views
0
votes
1
reply
70
views
updated 2 hours ago by
GenoMax
142k
•
written 6 hours ago by
mohamadzare6022
▴
10
0
votes
0
replies
70
views
0
votes
0
replies
64
views
0
votes
0
replies
61
views
14 hours ago by
Jacob
•
0
0
votes
1
reply
55
views
0
votes
0
replies
57
views
0
votes
0
replies
38
views
4 hours ago by
emmanouil.a
▴
120
0
votes
0
replies
35
views
0
votes
0
replies
29
views
2 hours ago by
adarsh_pp
▴
40
0
votes
0
replies
27
views
Recent Votes
Recent Locations •
All
Oviedo, just now
United Kingdom, just now
Philadelphia, just now
Bologna, just now
Milan, Italy, 1 minute ago
UK, 2 minutes ago
UK, 5 minutes ago
Recent Awards •
All
Popular Question to
adarsh_pp
▴
40
Popular Question to
liyong
▴
80
Popular Question to
Shicheng Guo
★
9.4k
Popular Question to
reza
▴
300
Recent Replies
Comment: Error with BiocParallel. No barcodes files found
by
NTerway
•
0
Thank you for your help! I will try this out. I am trying to implement a collaborator's pipeline on my data and they only use SCE, scran an…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
ATpoint
82k
Once you have the counts any n is possible on a regular analysis-grade laptop or workstation. Even hundreds of samples. It's really the pre…
Comment: Error with BiocParallel. No barcodes files found
by
ATpoint
82k
You just tell the function to remove the prefix for you. Not on the actual file. Then you can add this information to the SCE, like `sce$da…
Comment: log2 fold change in RNA-seq analysis
by
May Ling
•
0
Thank you for your response. I apologize if my understanding is incomplete. I would like to ask: if I have a fold change of -2, how can I c…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
Yeah, a standard 3x3 bulk experiement is very analysable on pretty much most good consumer laptops these days.
Comment: Error with BiocParallel. No barcodes files found
by
NTerway
•
0
Thanks for the explanation! If I remove the prefix (GSM3972009_69_) then I will lose the sample annotation and cannot track them. I am more…
Answer: Error with BiocParallel. No barcodes files found
by
ATpoint
82k
The function assumes simply barcodes.tsv(.gz), genes.tsv(.gz) and matrix.mtx(.gz), without additional pre- or suffixes by default. You can …
Comment: How to interpret this plotMDS of three disease clusters?
by
ATpoint
82k
arrayWeights is imo always a good idea with human (or generally large) cohorts. What you can also do is to use something like sva to estima…
Comment: Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Po
by
GenoMax
142k
Not what you want to hear but it is possible that the Ab data is not good quality as the cellranger message says (assuming everything ran p…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
ATpoint
82k
Bulk RNA-seq is way less intensive in every regard than single-cell. Whatever you buy, be sure it's upgradable, meaning, no Mac :)
Answer: Unexpected read length from NGS
by
GenoMax
142k
If the provider does scanning/trimming of the data before delivery then it is possible that some of the reads were trimmed due to short ins…
Comment: Is it possible to run CibersortX with a very small number of samples?
by
Aspire
▴
330
Thanks. I have checked that all files are tab delimited, with the first column named Gene_Symbols, with capitalized gene symbols, and no qu…
Answer: samtools write-index
by
aw7
▴
310
Someone, presumably you, opened an [issue][1] for the same problem. The answer was:
> This problem should be fixed in htslib 1.20 (incl…
Traffic: 2498 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6