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121,935 results • Page
124 of 2439
Sort: Rank
Rank
Views
Votes
Replies
0
votes
3
replies
1.6k
views
10x GEX and ADT data analysis in Seurat
Seurat
scRNA-seq
ADT
Cite-seq
GEX
updated 17 months ago by
ATpoint
89k • written 17 months ago by
gdfsnkfns
• 0
2
votes
6
replies
1.6k
views
Correcting coordinates in gff3 file
awk
unix
gff3
python
updated 17 months ago by
Matthias Zepper
5.1k • written 17 months ago by
hulu
• 0
0
votes
4
replies
850
views
Questions about transfering wgs file to reference genome file
fasta
cram
genome
17 months ago by
me
• 0
1
vote
2
replies
1.0k
views
WGCNA
PCA
WGCNA
EIGENGENE
17 months ago by
rajasekargutha
▴ 60
4
votes
6
replies
2.4k
views
CompressedGRangesList don't have seqLengths: BiocParallel errors: Error in covRleFromGR(x, weight = weight, ignore.strand = ignore.strand): Seqlength…
gff3
GenomicRanges
ORFik
GenomicFeatures
updated 17 months ago by
GenoMax
154k • written 2.7 years ago by
Estefania
▴ 30
1
vote
2
replies
1.1k
views
Error when i use EVidenceModeler to do the genome annotation
EVidenceModeler
annotation
genome
16 months ago by
Wren
• 0
0
votes
3
replies
877
views
BLASTP web- <20aa peptides- interpreting results/ minimum E value?
blast
peptide
blastp
alignment
updated 17 months ago by
GenoMax
154k • written 17 months ago by
neish
• 0
3
votes
5
replies
1.6k
views
Having trouble building VG in Ubuntu
vg
updated 17 months ago by
Ram
45k • written 17 months ago by
ayushm64
• 0
1
vote
5
replies
3.7k
views
Shannon vs Simpson for TCR diversity estimation
TCR
shannon
simpson
updated 17 months ago by
ATpoint
89k • written 6.5 years ago by
CY
▴ 750
1
vote
0
replies
487
views
News:
Online Live Course: Introduction to Machine Learning in R
Rstats
TScourses
Rlang
Rproject
updated 17 months ago by
Ram
45k • written 17 months ago by
Transmitting Science communication
▴ 70
1
vote
0
replies
484
views
DESeq2 comparing timepoints within treatment and account for background control change
RNAseq
DEG
DESeq2
updated 17 months ago by
Ram
45k • written 17 months ago by
ian.will
▴ 30
2
votes
0
replies
447
views
News:
Online Course Generalised Additive Mixed Models (GAMM): modelling space-time correlation structures
GAMM
Rlang
Rstats
Rproject
updated 17 months ago by
Ram
45k • written 17 months ago by
Transmitting Science communication
▴ 70
2
votes
0
replies
468
views
News:
Online Course Introduction to Network Analysis and Modelling
Python
Network-Analysis
updated 17 months ago by
Ram
45k • written 17 months ago by
Transmitting Science communication
▴ 70
1
vote
3
replies
1.1k
views
Merging vcf files with bcftools ([E::get_intv] Failed to parse TBX_VCF, was wrong -p [type] used?)
vcf
tabix
bcftools
updated 17 months ago by
Pierre Lindenbaum
166k • written 17 months ago by
giulia.cosenza
▴ 20
0
votes
0
replies
460
views
Surrogate variable analysis for paired RNA seq experiment
sva
rnaseq
ruvseq
17 months ago by
nhaus
▴ 420
1
vote
3
replies
714
views
True variants selection
vcf
bcftools
updated 17 months ago by
dthorbur
★ 3.1k • written 17 months ago by
maevalefeuvre
• 0
0
votes
1
reply
505
views
How to reveal real AF of variant if duplicates can't be removed (such as in amplicon-seq)
allele-frequency
amplicon-seq
updated 17 months ago by
Ram
45k • written 17 months ago by
CY
▴ 750
0
votes
0
replies
429
views
Creating a BSgenome data package from a NCBI assembly - Virus
Virus
BSgenomeForge
BSgenome
R
17 months ago by
JirMan
▴ 40
2
votes
7
replies
3.2k
views
KEGG analysis in R
KEGG
clusterProfiler
Zea-mays
updated 16 months ago by
letizia.ottaviani
• 0 • written 17 months ago by
san96
▴ 190
0
votes
0
replies
687
views
BETA whether on the same scale when performing rank-based inverse normal transformation on the same type of phenotype data for two cohorts separately…
normal
inverse
transformation
17 months ago by
Isaac
• 0
2
votes
3
replies
1.3k
views
Why is DESeq2 normalization making my top feature have identical values across samples?
DESEQ2
WGCNA
17 months ago by
DNAngel
▴ 260
2
votes
2
replies
1.0k
views
Creating a cytoscape bubble figure
cytoscape
network
GO
17 months ago by
Dave Carlson
★ 2.2k
3
votes
3
replies
1.3k
views
Disrupting a gene interaction network
cytohubba
cytoscape
sequences
mRNA
17 months ago by
george
• 0
3
votes
1
reply
761
views
ClueGO is not showing custom data
ClueGo
Cytoscape
updated 17 months ago by
Scooter
▴ 310 • written 18 months ago by
Nicolas
• 0
2
votes
7
replies
7.6k
views
9 follow
problem with HOMER findMotifsGenome.pl
HOMER
ATAC-Seq
updated 17 months ago by
kaaz
• 0 • written 7.0 years ago by
ste.lu
▴ 80
5
votes
3
replies
1.2k
views
Best genome browser for manual curation of gene structure(s)?
structure
gene
updated 17 months ago by
cmdcolin
★ 4.3k • written 17 months ago by
William
★ 5.4k
1
vote
7
replies
7.3k
views
How do I plot several sns.clustermap heatmaps in a single figure?
python
seaborn
updated 17 months ago by
Wubin
▴ 50 • written 4.9 years ago by
roy.granit
▴ 890
0
votes
1
reply
599
views
Human Methylation Infinum Bead - probe to gene mapping
TCGA
pancan
data
updated 17 months ago by
Zhenyu Zhang
★ 1.3k • written 17 months ago by
shrutidesai0208
• 0
2
votes
5
replies
4.6k
views
Is it just me or has www.ensembl.org been broken for a while?
ensembl
updated 15 months ago by
joshua.theisen
▴ 30 • written 17 months ago by
gene_biostars
▴ 10
0
votes
0
replies
408
views
Installing SALSA and HIRise scafolding software
scafolding
SALSA
genome-assembly
HIRise
updated 17 months ago by
Ram
45k • written 17 months ago by
Lissa Cruz Saavedra
• 0
0
votes
1
reply
564
views
Extract Di-nucleotide/Tri-Nucleotide substitution frequencies from MSA/VCF file
MSA
substitution
VCFstats
Evolution
VCF
updated 17 months ago by
Pierre Lindenbaum
166k • written 17 months ago by
Saran
▴ 50
0
votes
2
replies
807
views
Abundance Calculation in MetaPhlAn 3: Methodology, TPM or RPKM, and the Treatment of Unclassified Reads
MetaPhlAn
Normalization
RPKM
TPM
17 months ago by
ramin.k2013
• 0
0
votes
0
replies
424
views
Normalization in Metagenomics Sequencing By Total Number of Reads: Pre- or Post-Filtering and the Role of Unclassified Reads
Normalization
Metagenomics
updated 17 months ago by
Ram
45k • written 17 months ago by
ramin.k2013
• 0
2
votes
3
replies
1.0k
views
Finding variants within a subset of a BAM file
variant
calling
16 months ago by
ramiro.barrantes
▴ 60
2
votes
1
reply
1.6k
views
convert h5ad to rds
single-cell
transcriptome
updated 17 months ago by
bk11
★ 3.1k • written 17 months ago by
lucas.aleixoleal17
• 0
1
vote
2
replies
1.3k
views
Error during demultiplexing by bcl2fastq
bcl2fastq
updated 17 months ago by
GenoMax
154k • written 17 months ago by
daffodil
▴ 10
0
votes
2
replies
850
views
Help with generating annotation database for dog genome to use in ANNOVAR for variant annotation of dog tumor sequences
ANNOVAR
16 months ago by
sainavyav22
• 0
0
votes
1
reply
1.0k
views
Clair3 very low F1 INDEL score
ont
clair3
nanopore
wgs
updated 17 months ago by
yussab
▴ 100 • written 3.2 years ago by
Satrio
• 0
0
votes
9
replies
2.0k
views
99.9999% of Q30 bases is normal?
RNA-seq
fastp
updated 17 months ago by
LauferVA
4.8k • written 17 months ago by
Aki
▴ 20
0
votes
0
replies
492
views
Issue while using circle_dat function from GOplot in R
GOplot
R
17 months ago by
ckelly96
• 0
1
vote
7
replies
4.4k
views
fastp - can I trim specific adapters only?
trimming
fastp
adapter
17 months ago by
liorglic
★ 1.5k
0
votes
2
replies
818
views
Can't figure out plink --sample-diff
plink
17 months ago by
curious
▴ 890
0
votes
1
reply
6.2k
views
How to compute a one tail (one way) log rank test for survival?
R
survival
hypothesis-testing
log-rank
updated 17 months ago by
jack
• 0 • written 9.0 years ago by
denalitastic
▴ 30
0
votes
1
reply
767
views
Geneious Prime (Consense Sequence)
Consense
Sequence
updated 17 months ago by
GenoMax
154k • written 17 months ago by
Reno
• 0
1
vote
2
replies
803
views
convert a seurat object to expressionset
Seurat
17 months ago by
Nona
▴ 90
2
votes
0
replies
414
views
News:
Course Functional Programming with R
Rstats
TScourses
Rproject
KeepLearning
Rlang
17 months ago by
Transmitting Science communication
▴ 70
1
vote
1
reply
504
views
Shared pathways up in disease blood and tissue relative to healthy blood (scRNA-seq)
scRNA-seq
updated 17 months ago by
ATpoint
89k • written 17 months ago by
Lucy
▴ 170
2
votes
4
replies
1.2k
views
Per Base Sequence Content - continuous raise of G%
fastqc
updated 17 months ago by
GenoMax
154k • written 17 months ago by
pl.terzian
• 0
0
votes
0
replies
501
views
Inferring cell identity/genotype in single cells with missing information
cell
seurat
single
score
17 months ago by
txema.heredia
▴ 250
0
votes
2
replies
832
views
fmols for nanopore library loading
nanopore
sequencing
libraryprep
fmol
LSK114
updated 17 months ago by
GenoMax
154k • written 17 months ago by
njornet
▴ 20
121,935 results • Page
124 of 2439
Recent Votes
What happened to biomart.org
Answer: allele frequency in VCF
Understanding terms, specifically relating to GT:PL in a VCF file
Comment: Looking to learn practical bioinformatics
Answer: WGCNA package in R: dendogram plot error
Answer: Does this adapter content from fastqc to warrant trimming prior to alignment?
Answer: Does this adapter content from fastqc to warrant trimming prior to alignment?
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Recent Replies
Comment: real time PCR data analysis
by
ATpoint
89k
I cannot guide on GraphPad. It's commercial software so better contact their support.
Answer: Linear regression vs DESeq2 models for DEG analysis
by
swbarnes2
15k
DE genes in bulk RNASeq is what DESeq2 was made for, so why wouldn't you use it? RPKM is not an appropriate normalization method here. M…
Comment: What happened to biomart.org
by
Jeremy Leipzig
23k
same with geuvadis.org
Comment: real time PCR data analysis
by
Gordon Smyth
★ 8.3k
Are you doing qPCR for one gene or for several genes?
Comment: protein interaction prediction
by
Fatemeh
▴ 50
Thanks very much for your help. I need to try it.
Comment: real time PCR data analysis
by
Fatemeh
▴ 50
Thanks a lot for the link. The problem is that with this repeated measures, if you put the data from different donors in different rows, fo…
Answer: What happened to biomart.org
by
GenoMax
154k
Looks like EBI let the domain registration expire. The domain was picked up by someone else and turned into the site OP mentions. There use…
Comment: Hifiasm is getting killed while trying to assemble a genome
by
GenoMax
154k
> I want to reconstruct the assembly using the pacbio reads only. Is there a paper associated with this data? If the original publication/…
Comment: real time PCR data analysis
by
ATpoint
89k
I think the keyword to go after here is repeated measures https://www.graphpad.com/guides/prism/latest/statistics/repeated_measures.htm
Answer: What happened to biomart.org
by
ATpoint
89k
Maybe I am missing sth because I've never used the website but only the API and R interface, but I think the website has always been https:…
Comment: protein interaction prediction
by
Fatemeh
▴ 50
It is most probably binding to a protein. Its function is somehow known though, which is good . Thanks very much for the link. I will have …
Comment: Looking to learn practical bioinformatics
by
Upesh
▴ 20
ATpoint, you are absolutely bang on. In fact, my intention is not to switch careers but to connect with this field and build tools/systems …
Comment: Hifiasm is getting killed while trying to assemble a genome
by
pranavdatar01
• 0
I am trying to reproduce the assembly from the raw pacbio reads. I want to reconstruct the assembly using the pacbio reads only.
Comment: Does this adapter content from fastqc to warrant trimming prior to alignment?
by
ATpoint
89k
I would trim it. Two percent is not much but with WGS, say you have 400mio reads per sample or so, it's still millions of reads that theore…
Comment: Looking to learn practical bioinformatics
by
ATpoint
89k
> How do I connect the dots to a grand vision of drug discovery, DNA analysis, disease etc. Despite that computational-driven data analys…
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