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121,901 results • Page
623 of 2439
Sort: Rank
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Votes
Replies
0
votes
4
replies
1.9k
views
Converting multiple cram files to fastq files.
cram
fatsq
updated 4.2 years ago by
Ram
45k • written 4.2 years ago by
Rose
• 0
1
vote
3
replies
2.4k
views
igvtools not running, issue with java
igv
jdk
igvtools
java
4.2 years ago by
williamtmills
▴ 20
2
votes
3
replies
1.6k
views
Convert BAM files to FastQ
fastq
bedtools
samtools
bam
updated 4.2 years ago by
swbarnes2
15k • written 4.2 years ago by
nrk_02
▴ 10
7
votes
11
replies
3.2k
views
6 follow
how to convert to computational biology from bioinformatics
software
computational-biology
algorithm
updated 2.5 years ago by
Ram
45k • written 4.2 years ago by
biohubx
• 0
0
votes
0
replies
708
views
HTseq low counts
snakemake
RNASeq
htseq
4.2 years ago by
aka
▴ 10
0
votes
0
replies
1.4k
views
Split Y-Axis of a manhattan plot in qqman (using R)
manhattan
R
genomics
4.2 years ago by
tacrolimus
▴ 150
3
votes
3
replies
1.1k
views
Why split fastq files?
fastq
alignments
ngs
bwa
updated 4.2 years ago by
GenoMax
153k • written 4.2 years ago by
shpak.max
▴ 60
0
votes
0
replies
1.2k
views
How to get transcript level estimates in TPM from TCGA BRCA
RNA-Seq
BRCA
TCGA
4.2 years ago by
StartR
▴ 30
0
votes
3
replies
2.3k
views
GNU plot problem
psmc
bash
4.2 years ago by
gubrins
▴ 350
0
votes
2
replies
1.3k
views
CPM and FPKM for machine learning
RNA-seq
updated 4.2 years ago by
seidel
11k • written 4.2 years ago by
bioinfo
▴ 60
1
vote
6
replies
2.7k
views
biomaRt does not convert entrez gene id to ensembl gene id
biomaRt
ensembl
entrez
NCBI
updated 3.9 years ago by
Mike Smith
★ 2.1k • written 4.2 years ago by
Bane
▴ 30
2
votes
8
replies
2.1k
views
GC content of specific regular expression
GC-content
updated 13 months ago by
Ram
45k • written 4.2 years ago by
isha.lily20
▴ 10
0
votes
5
replies
1.5k
views
Samtools Flagstat Comments
Samtools
BAM
Rna-Seq
Alignment
updated 4.2 years ago by
GenoMax
153k • written 4.2 years ago by
santos48
▴ 40
0
votes
0
replies
1.8k
views
Job:
PhD scholarship in Yeast Metabolic Engineering
Metabolic
RNAseq
Engineering
proteomics
DNAseq
4.2 years ago by
info
▴ 80
1
vote
5
replies
2.3k
views
Install Kent Source Archive Binaries
faToTwoBit
4.2 years ago by
kacollier
▴ 30
0
votes
1
reply
898
views
Single-cell atlas
rnaseq
singlecell
seurat
updated 4.2 years ago by
jared.andrews07
★ 19k • written 4.2 years ago by
barix
▴ 20
0
votes
4
replies
3.4k
views
the relationship of ATAC-seq and H3K27ac
and
ChIP-seq
ATAC-seq
4.2 years ago by
biohubx
• 0
0
votes
0
replies
710
views
Visualization of the cell cycle for ESC (or iPSC) from scRNA-seq data
cell
cycle
ESC
4.2 years ago by
Alexander
▴ 220
0
votes
0
replies
1.8k
views
How to index a genome.gff file? SNPcalling
snp
artemis
tabix
prokka
gff
updated 4.2 years ago by
ATpoint
89k • written 4.2 years ago by
BATMAN
• 0
3
votes
2
replies
1.6k
views
gVCF size human genome
variant
Gatk
gvcf
4.2 years ago by
quentin54520
▴ 120
0
votes
1
reply
939
views
Adding insect abundance data to SparCC Analysis
Microbiome
metagenomics
16S
microbiome
SparCC
updated 4.2 years ago by
Kevin Blighe
89k • written 4.2 years ago by
GlycineMax
▴ 10
4
votes
4
replies
2.2k
views
setting genotypes to missing
genotype
vcf
impute
4.2 years ago by
raalsuwaidi
▴ 110
2
votes
2
replies
1.3k
views
Unknown trimmer, paired end, other solutions not working
trimmomatic
software
error
updated 4.2 years ago by
GenoMax
153k • written 4.2 years ago by
Archan
• 0
0
votes
2
replies
1.0k
views
Needle error - cannot get second filename
needle
bash
4.2 years ago by
kaixas
• 0
3
votes
1
reply
8.0k
views
What is the difference between covariate with fixed effect and random effect?
covariate
mlm
componentsvariance
gwas
updated 4.2 years ago by
atorreso
▴ 130 • written 4.2 years ago by
Star:)
▴ 50
2
votes
3
replies
1.9k
views
Converting .delta file from NUCMER to format accepted by IGV? (.bam/.vcf)
NUCMER
IGV
SAMtools
MUMMER
4.2 years ago by
Joanna
• 0
2
votes
4
replies
2.9k
views
16S rRNA merge forward and reverse primers
16S
taxonomy
rRNA
microbiology
primers
3.9 years ago by
ddowlin
▴ 70
5
votes
5
replies
9.3k
views
7 follow
Mirror Manhattan Plot
qqman
manhattan
GWAS
plot
R
updated 4.2 years ago by
Jimbou
▴ 960 • written 8.7 years ago by
jennysjaarda
▴ 10
0
votes
1
reply
1.0k
views
Flagging Blacklisted regions instead of deleting them.
regions
Blacklisted
updated 4.2 years ago by
seidel
11k • written 4.2 years ago by
akshitiz09
▴ 20
0
votes
12
replies
7.2k
views
DiffBind 3.2 error
DiffBind
R
ChIP-seq
updated 4.2 years ago by
Learner
• 0 • written 4.3 years ago by
Huang Zhihao
• 0
1
vote
0
replies
1.3k
views
What does gene ratio and count mean as output of the gseGO function?
clusterProfiler
RNAseq
4.2 years ago by
Wocher33
▴ 10
5
votes
6
replies
2.9k
views
issue with visualising cladogram/phylogenetic tree with multiple sequence alignment data in R?
r
bioconductor
alignment
R
sequencing
updated 4.2 years ago by
Biodeer
▴ 50 • written 6.3 years ago by
Kumar
▴ 120
0
votes
1
reply
629
views
Sample size required
statistics
updated 4.2 years ago by
ATpoint
89k • written 4.2 years ago by
Lital
• 0
0
votes
2
replies
1.0k
views
Comparing two ratios between two experiments.
comparisons
phosphoproteomics
ratios
4.2 years ago by
Vanish007
▴ 50
1
vote
2
replies
1.3k
views
Metatrancriptomics : Pfam Database for viralVerify after rnaviralspades running
Viralverify
database
Pfam
HMMer
4.2 years ago by
bioAddict
• 0
2
votes
1
reply
935
views
Why is age and sex added as covariates in GWAS regressions?
GWAS
updated 4.2 years ago by
Kevin Blighe
89k • written 4.2 years ago by
FeedMeDumplings
• 0
1
vote
2
replies
2.4k
views
[R] UpSetR - How to make UpSetPlot only show the highest level interactions?
r
upsetr
upsetplots
updated 4.2 years ago by
Basti
★ 2.1k • written 4.2 years ago by
Michael
▴ 10
7
votes
4
replies
1.3k
views
How to extract variants falling in a genomic window from a VCF?
vcf
updated 4.2 years ago by
GenoMax
153k • written 4.2 years ago by
francois
▴ 90
1
vote
2
replies
1.4k
views
missing REF and ALT in vcf
REF
SNP
VCF
ALT
4.2 years ago by
raalsuwaidi
▴ 110
1
vote
0
replies
930
views
plot Roary results for oan genome analysis
R
Roary
updated 2.2 years ago by
Ram
45k • written 4.2 years ago by
sharmatina189059
▴ 110
0
votes
1
reply
1.5k
views
Tool:
Compi: Application Framework for Portable Computational Pipelines
pipeline
workflow-management-system
docker
updated 2.3 years ago by
Ram
45k • written 5.9 years ago by
Hugo
▴ 400
0
votes
2
replies
1.3k
views
Wobbly average GC profile spanning entire R1 read length; what the cause and is it bad
fastq
qc
4.2 years ago by
William
★ 5.4k
0
votes
1
reply
750
views
somatic variant calling
Variant
calling
updated 4.2 years ago by
ATpoint
89k • written 4.2 years ago by
Elisa
• 0
6
votes
7
replies
3.8k
views
Extract CDS from maker gff
CDS
maker2
annotation
gff
updated 2.5 years ago by
lieven.sterck
15k • written 4.2 years ago by
Sarah
▴ 60
0
votes
0
replies
1.0k
views
News:
FINAL CALL :: Online Course - A Practical Introduction to NGS Data Analysis (July 14-16, 2021)
NGS
workshop
updated 2.5 years ago by
Ram
45k • written 4.2 years ago by
David Langenberger
11k
1
vote
8
replies
3.5k
views
unable to impute with beagle after filtering VCF table using vcftools
genomics
bioingormatics
vcftools
beagle
updated 4.2 years ago by
Nai
▴ 50 • written 4.4 years ago by
ziv_attia
• 0
5
votes
4
replies
1.4k
views
orthology enrichment
orthology
updated 13 months ago by
Ram
45k • written 4.2 years ago by
isha.lily20
▴ 10
0
votes
1
reply
1.2k
views
import PCA data into R as dataset to plot
PCA
updated 4.2 years ago by
Ram
45k • written 4.2 years ago by
sara_johem
• 0
0
votes
1
reply
1.2k
views
How can I use a short sequence as a probe to fish out sequences have the same part out from raw illumina paired reads files?
rna
paired
updated 4.2 years ago by
swbarnes2
15k • written 4.2 years ago by
wang-yanfang
• 0
3
votes
2
replies
2.4k
views
Flow cytometry and Cytof data analysis
Cytof
data
analysis
flow
cytometry
4.2 years ago by
rashmirao962
▴ 10
121,901 results • Page
623 of 2439
Recent Votes
Shift in RNA 3' position, Term-Seq vs RNASeq
Comment: STARlong for pacbio Isoseq reads
Comment: Automatically annotating a feature using a genbank file
Answer: Is this PCA plot correct??
Answer: Is this PCA plot correct??
Comment: Is this PCA plot correct??
Subsetting before QC in Spatial Transcriptomics
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okiedokie1208
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Answer: STARlong for pacbio Isoseq reads
by
YuZJ
• 0
Hello, you may try deSALT (DOI: 10.1186/s13059-019-1895-9, GitHub: ydLiu-HIT/deSALT) or uLTRA (DOI: 10.1093/bioinformatics/btab540, GitHub:…
Comment: Shift in RNA 3' position, Term-Seq vs RNASeq
by
acvill
▴ 350
Without knowing the details of the Term-seq method, could the 38 nt shift be accounted for by the difference in the position of the ribosom…
Comment: STARlong for pacbio Isoseq reads
by
Buffo
★ 2.4k
Thanks, I'll give it a try.
Comment: STARlong for pacbio Isoseq reads
by
GenoMax
153k
You could give `mapPacBio.sh` from BBMap suite a try while you wait for other answers. That works for reads up to 6 kb. BBMap has a new hom…
Comment: STARlong for pacbio Isoseq reads
by
Buffo
★ 2.4k
The average is 1.8kb. I was planning to post it on GitHub, but it seems like the repository has been unattended for the last months, so I d…
Comment: Getting just fastqc.zip file?
by
GenoMax
153k
Yes, You can either use it on command line or use the graphical user interface.
Comment: STARlong for pacbio Isoseq reads
by
GenoMax
153k
What is the average length of your reads? That error sounds like a bug, so you may want to post that on `STAR` repo as an issue. I assume …
Comment: Getting just fastqc.zip file?
by
pourhangleila95
• 0
I want to perform fastQC on my data.Do you use fastQC software for doing it?
Comment: Whole Genome Sequencing IG regions
by
719
• 0
Sorry for the poor wording of the original post, also should say I really appreciate people taking a moment to look at my post. My main con…
Comment: Is total miRNA in EV constant? Implications for library size normalization.
by
Thomas.H.Hampton
▴ 10
Thanks again for your carefully considered reply. You asked what I am hoping to learn from a DE analysis. Autologous stem cells are well …
Comment: Subsetting before QC in Spatial Transcriptomics
by
Kent
▴ 30
Hi ATpoint, thank you very much for the helpful insight! I'm still doing some literature reading on this myself, but have you come across a…
Comment: Whole Genome Sequencing IG regions
by
ATpoint
89k
The question is what you are after. Is this a disease setup, or...well, what is this all about. Generally, these regions are hotspots of hy…
Comment: Whole Genome Sequencing IG regions
by
Pierre Lindenbaum
166k
please define "interesting"
Comment: Hifiasm is getting killed while trying to assemble a genome
by
pranavdatar01
• 0
These are PacBio HiFi reads (total reads 5,237,005), and the total data size is 255 GB. I am the only user on the system.
Comment: Subsetting before QC in Spatial Transcriptomics
by
ATpoint
89k
Yes, absolutely. It is even (to me) a good practice to do (automated) crude celltype assignment first because QC metrics, such as number of…
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