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121,425 results • Page
1 of 2429
Sort: Rank
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0
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0
replies
4
views
Computing ka/ks ratio for individual genes.
ks
ka
evolution
selection
19 minutes ago by
Shakunthala Natarajan
• 0
0
votes
5
replies
179
views
Host Removal Issues Human/Dog with samples containing Eukaryotic species
Eukarotic
Mapping
Host
36 minutes ago by
Jon
• 0
0
votes
1
reply
65
views
Pharokka Error
error
annotating
viral
pharokka
genome
updated 27 minutes ago by
Mensur Dlakic
★ 29k • written 4 hours ago by
NIkita
• 0
0
votes
0
replies
39
views
Combining Metatranscriptomics (Mtx) and Metagenomics (Mgx)
metagenomics
reference-based
Metatranscriptomics
alignment
updated 3 hours ago by
GenoMax
152k • written 4 hours ago by
rk009
• 0
1
vote
1
reply
82
views
how to make a circular plot of variants
circular
plot
updated 10 hours ago by
Pierre Lindenbaum
166k • written 10 hours ago by
analyst
▴ 70
840
votes
170
replies
180k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 7 weeks ago by
Biostar
3.6k • written 8.5 years ago by
Istvan Albert
102k
2
votes
6
replies
188
views
GSEA analysis
RNA-seq
RNA
analysis
updated 8 hours ago by
GenoMax
152k • written 12 hours ago by
Ahmed
• 0
0
votes
0
replies
51
views
how to make go term plot and kegg pathway plot using InterProScan output
interproscan
12 hours ago by
analyst
▴ 70
1
vote
3
replies
311
views
Why little difference in RNAseq alignment when flipping forward and reverse strand with paired end reads
mRNA
rna-seq
bowtie
alignment
updated 21 hours ago by
swbarnes2
15k • written 1 day ago by
JourneyToAbyss
▴ 250
0
votes
1
reply
118
views
DESeq2 design for differential expression with 2 timepoints and 2 controls
Transcriptomic
Feeding
DESeq2
RNAseq
updated 21 hours ago by
swbarnes2
15k • written 1 day ago by
Cristina
• 0
1
vote
2
replies
165
views
Venn Diagram
VennDiagram
updated 22 hours ago by
WouterDeCoster
48k • written 1 day ago by
Nasim Gandomdoust
• 0
0
votes
1
reply
136
views
Questions about sample_accession_id and file_accession_id from EGA files
EGA
open
WGS
data
updated 1 day ago by
GenoMax
152k • written 1 day ago by
askif4
▴ 20
4
votes
3
replies
248
views
Example of ClinVar pathogenic variant where the reference allele is pathogenic?
snp
variant
pathogenic
clinvar
updated 1 day ago by
GenoMax
152k • written 2 days ago by
a615ebfb
▴ 60
0
votes
1
reply
146
views
Functional Analysis Using EMU Output – Has Anyone Tried This?
emu
updated 1 day ago by
andres.firrincieli
3.9k • written 2 days ago by
henrique.mouradias
• 0
1
vote
1
reply
154
views
featureCounts -t option not working in v2.0.8?
subread
featureCounts
RNAseq
updated 2 days ago by
rfran010
★ 1.5k • written 2 days ago by
atan
• 0
0
votes
1
reply
156
views
mm10 blacklist regions detailed information
sequencing
DNA
blacklist
updated 2 days ago by
GenoMax
152k • written 2 days ago by
annaA
▴ 10
0
votes
1
reply
155
views
identify unmapped regions
human
illumina
libraries
updated 2 days ago by
GenoMax
152k • written 2 days ago by
María José
▴ 10
0
votes
1
reply
254
views
martCheck - Error - You must provide a valid Mart object - for running HoneyBADGER for CNV computation
CNV
biomaRt
HoneyBADGER
2 days ago by
mete.han.celebi
• 0
0
votes
1
reply
197
views
How to Specify Power Parameter for Adjacency in modulePreservation() (WGCNA)
module
analysis
WGCNA
preservation
updated 2 days ago by
andres.firrincieli
3.9k • written 2 days ago by
Berkan
• 0
0
votes
1
reply
175
views
filters used in mutation discovery from RNAseq data
filter
RNAseq
mutation
updated 2 days ago by
GenoMax
152k • written 2 days ago by
htchd
▴ 20
2
votes
3
replies
298
views
Trouble Using GEO Files with Seurat
seurat
GEO
scRNA
2 days ago by
Jamie
• 0
0
votes
0
replies
153
views
Knowledge Graph for AI
KnowledgeGraph
biotech
AI
SPOKE
Bioinformatician
2 days ago by
Cornelio
• 0
2
votes
2
replies
892
views
How to convert from .spec to .bam?
bam
file-formatting
spec
updated 3 days ago by
Daniel Lai
▴ 10 • written 4.8 years ago by
gmora
▴ 30
2
votes
1
reply
205
views
Insert size is zero
rna-seq
fastp
insert
size
updated 3 days ago by
GenoMax
152k • written 3 days ago by
SpliceAndScript
• 0
0
votes
3
replies
312
views
Should matched samples (not paired) be included in the DESeq2 design model?
edgeR
limma
DESeq2
updated 2 days ago by
i.sudbery
21k • written 3 days ago by
marieke
• 0
0
votes
4
replies
307
views
Trajectory Analysis scRNA-seq with one cell type and two developmental stages
cellrank
palantir
pseudotime-trajectory-analysis
scrna-seq
trajectory-analysis
2 days ago by
npont
▴ 10
0
votes
1
reply
554
views
How to run trajectory analysis with only one cell type using palantir?
palantir
scanpy
updated 3 days ago by
npont
▴ 10 • written 11 months ago by
bioinfo
▴ 160
0
votes
1
reply
191
views
should I remove the transcripts from annotate peak region in ATAC-seq
ATAC-seq
updated 2 days ago by
rfran010
★ 1.5k • written 3 days ago by
QX
▴ 70
3
votes
2
replies
274
views
DESeq2 LRT test with multifactorial design
test
design
multifactorial
LRT
3 days ago by
lessismore
★ 1.4k
1
vote
0
replies
768
views
Tool:
Created a better alternative to clunky gene databases, with functions that are consolidated from various gene databases. It's your one-stop shop for …
browser
export
data
search
gene
3 days ago by
Krish
• 0
1
vote
0
replies
180
views
Tool:
Containerized NGS Data Processing Pipelines
rna-seq
atac-seq
pipeline
chip-seq
docker
3 days ago by
Noah
▴ 10
0
votes
1
reply
209
views
Scvi - Integration
integration
singlecell
scvi
updated 3 days ago by
antonioggsousa
3.3k • written 4 days ago by
t.foskolos
• 0
0
votes
0
replies
178
views
Genotype Imputations Question
vcf
imputations
genomics
4 days ago by
Jonathan
• 0
0
votes
1
reply
223
views
Clarification of options --keep-dup: all vs. -f FRAG in MACS3
human
libraries
illumina
callpeak
macs3
updated 4 days ago by
ATpoint
88k • written 4 days ago by
María José
▴ 10
6
votes
2
replies
515
views
Should I perform integration to correct batch effects?
batch
effect
updated 4 days ago by
antonioggsousa
3.3k • written 8 days ago by
han
▴ 20
2
votes
1
reply
239
views
Batch correction in TNBC epithelial cells
tnbc
seurat
epithelialCells
scRNAseq
BatchEffect
updated 4 days ago by
ATpoint
88k • written 4 days ago by
TNF_alpha
• 0
2
votes
5
replies
482
views
7 follow
Best way to install R packages on an HPC?
R
HPC
updated 4 days ago by
Charles Plessy
★ 2.9k • written 5 days ago by
ChumBucket2024
▴ 10
2
votes
2
replies
306
views
how to use filter_vep to filter vcf file
vcf
filter
3 days ago by
htchd
▴ 20
1
vote
2
replies
300
views
How to interpret genomescope to help remove duplicates
assembly
hifi
genomescope
4 days ago by
mohamadelian1996
• 0
0
votes
2
replies
275
views
Is it valid to stack brightfield and fluorescence channels in a CNN input?
fluorescent-microscopy
imaging
microscopy
updated 5 days ago by
LChart
5.0k • written 5 days ago by
Antonio
• 0
0
votes
0
replies
195
views
FusionCatcher cases where gene1 and gene 2 have 2 matches
fusions
fusioncatcher
5 days ago by
ieie
▴ 20
0
votes
2
replies
280
views
mgl tool index error
docking
molecular
error
4 days ago by
Mahesh
• 0
2
votes
3
replies
377
views
convert raw counts to TPM
R
updated 5 days ago by
dthorbur
★ 3.0k • written 5 days ago by
pinheirofabiano
▴ 130
1
vote
7
replies
436
views
Creating kraken2 custom database
kraken2
updated 5 days ago by
GenoMax
152k • written 5 days ago by
ramiro.barrantes
▴ 60
0
votes
0
replies
199
views
How to determine the coordinate of the transposon insertion on the reverse reading?
Tn-seq
transposon
blastn
5 days ago by
Елизавета
• 0
0
votes
6
replies
434
views
Single-cell RNA Sequencing - transcript abundance estimation using Alevin-Fry
Alevin-Fry
single-cell
sequencing
Salmon
RNA
2 days ago by
wooh
• 0
0
votes
0
replies
203
views
News:
Conservation Genomics course
PopulationGenomics
Ne
ConservationGenomics
5 days ago by
Physalia-courses
★ 2.6k
0
votes
3
replies
352
views
Snakemake Module Error: string indices must be integers
GATK
Snakemake
updated 5 days ago by
Jesse
▴ 870 • written 5 days ago by
andean
• 0
1
vote
3
replies
357
views
How do I generate a Manhattan plot like this?
data-visualization
manhattan-plot
5 days ago by
jianhua.mert
• 0
0
votes
2
replies
355
views
ATAC seq: from peaks to differential analysis ??
MACS2
DESeq2
ATACseq
updated 5 days ago by
rfran010
★ 1.5k • written 6 days ago by
Picasa
▴ 680
121,425 results • Page
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Answer: When and why is bwa aln better then bwa mem?
Comment: how to make a circular plot of variants
Comment: GSEA analysis
Answer: GSEA analysis
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Example of ClinVar pathogenic variant where the reference allele is pathogenic?
Answer: Example of ClinVar pathogenic variant where the reference allele is pathogenic?
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Recent Replies
Answer: Host Removal Issues Human/Dog with samples containing Eukaryotic species
by
Jon
• 0
Ideally yes, recover reads that are NOT host. Yet the Naegleri Fowleri has high similarity to human, so when I assemble the reads I don't…
Answer: Pharokka Error
by
Mensur Dlakic
★ 29k
Your problem seems to be with `phanotate` version. Generally speaking, that will be best resolved if you directly communicate with the auth…
Comment: Host Removal Issues Human/Dog with samples containing Eukaryotic species
by
GenoMax
152k
What is the main aim here? To recover reads that are NOT host. Using some % cutoff is not a great strategy and reads will map by chance be…
Comment: GSEA analysis
by
Ahmed
• 0
I believe you are right. My mistake! I am confusing NES with upregulation/downregulation, I think. It is a little confusing.
Comment: how to make a circular plot of variants
by
Pierre Lindenbaum
166k
https://circos.ca/tutorials/lessons/
Comment: GSEA analysis
by
nux
▴ 50
try log2FoldChange * -10logpvalue and if possible can you cross check it with the library in the link I sent earlier. How can you tell that…
Comment: GSEA analysis
by
Ahmed
• 0
I used featureCounts to get gene-level counts from RNA-seq BAMs, then ran DESeq2 to get log2FoldChanges and adjusted p-values. Then I took …
Comment: GSEA analysis
by
nux
▴ 50
no, walk me through your process and let's check how did you end up with different regulation. For visualization, you can check some ideas …
Comment: GSEA analysis
by
Ahmed
• 0
So, are you saying that I use something like ORA?
Answer: GSEA analysis
by
nux
▴ 50
Ca you elaborate the steps that you did for your analysis. For all just to make sense or to establish the context of your analysis, make su…
Comment: Host Removal Issues Human/Dog with samples containing Eukaryotic species
by
Jon
• 0
What are your thoughts of first Mapping my target reads at say 95% or higher first, then doing host removal of the remaining reads, and the…
Comment: Host Removal Issues Human/Dog with samples containing Eukaryotic species
by
Jon
• 0
Yes, I have read about that tool, as it appeared to be what I was looking for. I tried installing the BBMap on my Windows computer, but c…
Comment: Host Removal Issues Human/Dog with samples containing Eukaryotic species
by
GenoMax
152k
> I can only work with the tools available on Galaxy.org That requirement is likely going to make this very difficult to accomplish, ther…
Answer: Why little difference in RNAseq alignment when flipping forward and reverse stra
by
swbarnes2
15k
Bowtie doesn't know what read should go in what direction. It's aligning to genome, it will just flip things to make the alignment work. …
Answer: DESeq2 design for differential expression with 2 timepoints and 2 controls
by
swbarnes2
15k
Adding interactions is not correct for most of what you are doing. Time + treatment is what you want for 1 and 2. For questions like 3, m…
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