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Forum: Finding batch and outlayers
Pca plot
3 minutes ago by Tigran • 0
5
votes
3
replies
256
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 5 days ago by 141k • written 5 days ago by • 0
0
votes
0
replies
162
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
5 days ago by yxwucq • 0
4
votes
2
replies
291
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 12 days ago by 43k • written 12 days ago by ▴ 10
7
votes
7
replies
1.3k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 13 days ago by ★ 2.5k • written 14 days ago by • 0
1
vote
2
replies
343
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
11 days ago by Javier • 0
16
votes
10
replies
1.1k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 4 weeks ago by 5.3k • written 4 weeks ago by ★ 2.4k
1
vote
1
reply
222
views
Forum: Can I use this community for software development?
software-development
updated 4 weeks ago by 43k • written 4 weeks ago by • 0
5
votes
2
replies
389
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 4 weeks ago by 54k • written 4 weeks ago by • 0
0
votes
0
replies
175
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 5 weeks ago by 43k • written 5 weeks ago by • 0
1
vote
3
replies
478
views
Forum: Development plan for a bioinformatics database developer/coder
developer
6 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
689
views
Forum: Bioinformatics career transition
career forum
updated 6 weeks ago by ★ 16k • written 6 weeks ago by ▴ 20
4
votes
2
replies
480
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
7 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
770
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
7 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
162
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 7 weeks ago by 43k • written 7 weeks ago by ★ 1.2k
0
votes
2
replies
379
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
7 weeks ago by Dave Carlson ★ 1.7k
31
votes
12
replies
985
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
8 weeks ago by karlaarz ▴ 90
5
votes
4
replies
559
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 9 weeks ago by 141k • written 9 weeks ago by ▴ 10
1
vote
2
replies
370
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 9 weeks ago by 82k • written 9 weeks ago by ▴ 20
8
votes
7
replies
801
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 9 weeks ago by ▴ 120 • written 12 weeks ago by • 0
1
vote
0
replies
280
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 10 weeks ago by 161k • written 10 weeks ago by ★ 1.4k
4
votes
2
replies
377
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 11 weeks ago by ★ 5.9k • written 11 weeks ago by ▴ 10
8
votes
6
replies
2.8k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
203
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by 43k • written 3 months ago by ▴ 20
2
votes
3
replies
436
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
3 months ago by Ajay A Shettigar • 0
17
votes
11
replies
989
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.2k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 23 months ago by ▴ 20
26
votes
27
replies
2.0k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 3 months ago by ★ 5.9k • written 3 months ago by ▴ 40
7
votes
6
replies
734
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 3 months ago by 43k • written 3 months ago by ▴ 20
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
2
votes
1
reply
427
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
3 months ago by LauferVA 4.2k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
4
votes
3
replies
766
views
Forum: How to get started in bioinformatics
students
updated 3 months ago by 43k • written 3 months ago by ▴ 10
2
votes
2
replies
583
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 3 months ago by 43k • written 4 months ago by ▴ 40
0
votes
2
replies
3.2k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by 141k • written 4 months ago by ▴ 10
5
votes
3
replies
609
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 4 months ago by ★ 27k • written 4 months ago by ▴ 20
6
votes
2
replies
663
views
Forum: how to start Bioinformatics?
basics
updated 4 months ago by ★ 27k • written 4 months ago by • 0
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
928
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 4 months ago by 43k • written 4 months ago by • 0
1
vote
1
reply
490
views
Forum: want to work in open source project/project in bioinformatics
project
updated 4 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.0k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 4 months ago by 43k • written 5 months ago by • 0
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
624
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 5 months ago by ▴ 10 • written 5 months ago by ▴ 40
0
votes
0
replies
370
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 5 months ago by 43k • written 5 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
455
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
6 months ago by ishwarvh • 0
0
votes
0
replies
356
views
Forum: Automated Checks And QA
retrospective
6 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
1,000 results • Page 1 of 20
Recent Votes
Comment: NGS forensics: how to know if data is fabricated
A: Hard time trying to calculate Allele Frequency and DP from Platypus
Answer: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
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Recent Replies
Answer: Finding batch and outlayers by Tigran • 0
![enter image description here][1] [1]: /media/images/aa60b9ef-b08c-4093-9f0d-fb656a5c
Comment: NGS forensics: how to know if data is fabricated by benformatics 3.9k
Can you post an IGV screenshot of what this looks like?
Comment: NGS forensics: how to know if data is fabricated by benformatics 3.9k
Yeah some of the Illumina machines pre-trim adapters (not sure if this is still the standard). I've also definitely also seen quality-trimm…
Comment: PDB related issue by noodle ▴ 530
You can typically find much more useful info in the cif file, for example what you've found is called '_atom_site.pdbx_PDB_ins_code ' and d…
Comment: PDB related issue by Mensur Dlakic ★ 27k
The best way to find out is to read the original paper. My guess is that the residue has been crystallized in alternative conformations, wh…
Answer: NGS forensics: how to know if data is fabricated by Mensur Dlakic ★ 27k
I share the opinion by @philippbayer I have seen enough questionable things in life and in science to keep me permanently jaded, but there…
Comment: How many reads for WGS Sequencing? by Mensur Dlakic ★ 27k
I downloaded the data in two files from the link you provided. With this command: megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing? by Mensur Dlakic ★ 27k
If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
Comment: How many reads for WGS Sequencing? by Mensur Dlakic ★ 27k
I think you might be getting stuck on less relevant parts of my exercise. The most important point was that nothing is wrong with the data.…
Comment: What does it mean single base resolution in sequencing? by dsull ★ 5.9k
Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
Answer: What does it mean single base resolution in sequencing? by dsull ★ 5.9k
I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
Comment: Cannot process all the reads in a fast5 file? by chujie • 0
Hi, I met the same problem, have you found the solution?
Answer: What does it mean single base resolution in sequencing? by LauferVA 4.2k
Hi @fe3e6f65 , In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
Comment: PCA plot by LauferVA 4.2k
Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
Comment: NGS forensics: how to know if data is fabricated by Jeremy Leipzig 22k
i have the name ready: out**liar**
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