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459 results • Page 2 of 10
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19
votes
15
replies
2.0k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 11 months ago by 43k • written 6.0 years ago by 5.3k
57
votes
15
replies
6.3k
views
9 follow
Forum: Good practices while dealing with very large datasets
Big-Data Programming
updated 11 months ago by 43k • written 7.0 years ago by ★ 1.2k
21
votes
15
replies
2.1k
views
9 follow
Forum: Working abroad without a PhD
career
updated 11 months ago by 43k • written 4.6 years ago by ★ 1.4k
6
votes
15
replies
1.7k
views
Forum: PhD Bioinformatics on GWAS
SNP GWAS genome PHD
updated 11 months ago by 43k • written 4.3 years ago by ▴ 50
23
votes
15
replies
5.1k
views
6 follow
Forum: On the usage of Golang
assembly next-gen alignment
updated 11 months ago by 43k • written 5.6 years ago by ▴ 80
28
votes
15
replies
2.3k
views
12 follow
Forum: Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 11 months ago by 43k • written 5.8 years ago by ▴ 10
22
votes
15
replies
8.9k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 11 months ago by 43k • written 5.9 years ago by ▴ 80
9
votes
14
replies
2.4k
views
Forum: MS Bioinformatics Project
assembly sequencing alignment R SNP
updated 11 months ago by 43k • written 6.1 years ago by ▴ 10
3
votes
14
replies
2.1k
views
6 follow
Forum: Search for gene variants
next-gen
updated 11 months ago by 43k • written 5.7 years ago by 8.3k
73
votes
14
replies
4.3k
views
12 follow
Forum: Things you wish you knew when entering the bioinformatics field
pitfalls advice
updated 10 months ago by 43k • written 3.6 years ago by ★ 16k
17
votes
14
replies
3.3k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 11 months ago by ▴ 220 • written 2.3 years ago by ▴ 60
17
votes
14
replies
1.9k
views
10 follow
Forum: What would be the trend in next few years in NGS era?
NGS sequencing
updated 11 months ago by 43k • written 5.5 years ago by ▴ 800
19
votes
14
replies
2.1k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 7 months ago by 22k • written 7 months ago by ▴ 70
5
votes
14
replies
2.1k
views
Forum: immediate help needed for genome sequence analysis of bacteria
genome miseq webinar paired-end
updated 11 months ago by 43k • written 7.1 years ago by • 0
12
votes
14
replies
2.5k
views
8 follow
Forum: Recovering bam files after unknown deletion in the storage
photorec bam sequencing
written 4.9 years ago by ▴ 70
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 7 months ago by ▴ 10
11
votes
13
replies
4.5k
views
6 follow
Forum: NGS Data storage solutions for small organisations or big labs
big-data HTS storage NGS metagenomics
updated 11 months ago by 43k • written 4.9 years ago by ▴ 830
1
vote
13
replies
2.8k
views
Forum: Bioinformatics computer upgrade help
computer
updated 11 months ago by 43k • written 3.8 years ago by ▴ 50
15
votes
13
replies
4.2k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 18 months ago by ▴ 30
30
votes
13
replies
3.7k
views
11 follow
Forum: what is your daily must see bioinformatics web site?
learn technology
updated 10 months ago by 43k • written 9.6 years ago by 9.7k
4
votes
13
replies
1.7k
views
Forum: Looking for Participants: Meenta NovaSeq RunShare Beta
RunShare next-gen NovaSeq
updated 11 months ago by 43k • written 4.8 years ago by ▴ 10
23
votes
12
replies
2.0k
views
6 follow
Forum: Whole-Exome Analysis on a Mainstream Desktop PC
NGS whole-exome
updated 11 months ago by 43k • written 3.9 years ago by 11k
21
votes
12
replies
3.6k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
18
votes
12
replies
2.5k
views
6 follow
Forum: why GATK makes things more complicated ?
genomics
updated 11 months ago by 43k • written 5.2 years ago by ▴ 280
4
votes
12
replies
1.5k
views
Forum: publicly available data
arrayexpress GEO SRA
updated 11 months ago by 43k • written 4.6 years ago by ▴ 110
31
votes
12
replies
1.0k
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
9 weeks ago by karlaarz ▴ 90
22
votes
12
replies
1.8k
views
6 follow
Forum: What were the most impactful Bioinformatics papers published this year
papers publications
updated 11 months ago by 43k • written 4.4 years ago by ▴ 520
12
votes
12
replies
3.0k
views
Forum: Programming interview via Skype
programming-test interview
updated 11 months ago by 43k • written 5.9 years ago by ▴ 50
12
votes
12
replies
1.4k
views
7 follow
Forum: Who qualify as a world class bioinformatician as seen in job ads
world-class bioinformatician qualification
updated 11 months ago by 43k • written 4.1 years ago by • 0
8
votes
11
replies
1.8k
views
Forum: What makes a good bionformatics prediction service from the point of user experience?
server prediction-service
updated 11 months ago by 43k • written 6.5 years ago by ▴ 40
22
votes
11
replies
3.4k
views
Forum: ExpressionSet in the age of tidyverse
expressionset R bioconductor
updated 11 months ago by 43k • written 6.0 years ago by 13k
17
votes
11
replies
1.0k
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
23
votes
11
replies
2.1k
views
9 follow
Forum: file and directory management best practices
file-management
updated 10 months ago by 43k • written 3.7 years ago by ▴ 140
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 14 months ago by ▴ 150
15
votes
11
replies
4.7k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
21
votes
11
replies
2.0k
views
8 follow
Forum: what I want to do after the PhD?
PhD
updated 11 months ago by 43k • written 7.1 years ago by ★ 4.3k
14
votes
11
replies
6.8k
views
7 follow
Forum: Do you need a deduplication tool for FASTQ data in fastp?
deduplication fastp
updated 11 months ago by 43k • written 4.9 years ago by ★ 2.5k
13
votes
11
replies
4.7k
views
Forum: Anyone using Ubuntu on Windows (WSL)?
ubuntu wsl
updated 11 months ago by 43k • written 5.8 years ago by ▴ 250
9
votes
11
replies
3.3k
views
7 follow
Forum: Fields with biggest demand for bioinformatics?
career
updated 11 months ago by 43k • written 5.3 years ago by ▴ 200
10
votes
11
replies
4.2k
views
7 follow
Forum: What made you interested in Bioinformatics?
career
updated 11 months ago by 43k • written 6.4 years ago by ▴ 990
25
votes
11
replies
3.7k
views
6 follow
Forum: Site Use Guidelines
meta biostars Guidelines
updated 11 days ago by 43k • written 14.6 years ago by 100k
21
votes
11
replies
2.0k
views
Forum: Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 11 months ago by 43k • written 5.8 years ago by ★ 9.4k
18
votes
11
replies
2.4k
views
8 follow
Forum: Create a File format to store Variant
hgvs json variant
updated 11 months ago by 43k • written 5.9 years ago by ★ 2.4k
9
votes
10
replies
1.3k
views
6 follow
Forum: What's more clear, loops or functions?
R
updated 11 months ago by 43k • written 5.6 years ago by ▴ 110
2
votes
10
replies
1.2k
views
Forum: AddHealth Omics Data Source
AddHealthOmics
updated 11 months ago by 43k • written 5.4 years ago by • 0
15
votes
10
replies
2.4k
views
6 follow
Forum: help identifying bioinformatics operations tools
database next-gen operations genomics
updated 11 months ago by 43k • written 5.5 years ago by ▴ 80
26
votes
10
replies
4.4k
views
6 follow
Forum: Why not merging?
fastq sequencing
updated 11 months ago by 43k • written 6.8 years ago by 16k
10
votes
10
replies
1.7k
views
Forum: Doctor looking fertility for a collaborator for text mining on pubmed
text-mining fertility pubmed
updated 11 months ago by 43k • written 6.5 years ago by • 0
14
votes
10
replies
2.2k
views
Forum: WGBS alignment with BitMapperBS
BitMapperBS bismark
updated 11 months ago by 43k • written 4.8 years ago by ★ 9.4k
16
votes
10
replies
1.1k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 5 weeks ago by 5.3k • written 5 weeks ago by ★ 2.4k
459 results • Page 2 of 10
Recent Votes
Comment: GRCh38.gmap file
Answer: GRCh38.gmap file
Answer: GRCh38.gmap file
Answer: Design matrix Differential expression analysis
A: PCA plot from read count matrix from RNA-Seq
A: PCA plot from read count matrix from RNA-Seq
Answer: Design matrix Differential expression analysis
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Recent Awards • All
Autobiographer to Mathew ▴ 20
Commentator to dsull ★ 6.0k
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Recent Replies
Comment: Design matrix Differential expression analysis by Gordon Smyth ★ 7.1k
> When is it recommended to use the design <- cbind(Intercept=1,Group=c(-1,-1,-1,1,1,1)) It is not recommended. No such code appears anywh…
Comment: GRCh38.gmap file by lorena9132 ▴ 10
Hi Mathew, The .txt files provided in the link differ from the .gmap files, but after several attempts, I succeeded in generating the .gma…
Answer: GRCh38.gmap file by Mathew ▴ 20
Hi, I have spent some time searching for a GRCH38 map file for you. I have found a certain publication that uses a hg38 genetic map file…
Answer: Association study using SNP data by Mathew ▴ 20
Hi, You asked three questions so I will attempt to help the best I can **What should be the optimal GC score cutoff** It sounds li…
Comment: Design matrix Differential expression analysis by SHN ▴ 40
Thank you for your explanation. I am working on the cell lines and I am investigating the effect of the treatment on the expression level, …
Answer: Issues with Mixture file when using CIBERSORTx by vjanve • 0
I ran into same issue of "duplicated gene symbol(s)": 1: In CIBERSORTxFractions(sigmatrix = sigmatrix, mixture = mixture, : 22292 dup…
Comment: creating batch colum for batch correction by Expert ▴ 10
Thank you for your response. These samples were collected from the same patient at different time points(day 0, 8 and 18) and these are ind…
Comment: creating batch colum for batch correction by ATpoint 82k
Not sure I follow. Can you first of all describe which comparisons you want to make, pretending there was no batch effect at all. Write dow…
Comment: Pindel Segmentation Fault Error by Ruqaiya • 0
This the tool still problematic?
Comment: Segmentation error while using a tool by Ruqaiya • 0
Thank you for the advice, i'll keep that in mind.
Comment: Segmentation error while using a tool by Ruqaiya • 0
I am trying to reproduce some bioinformatics data from a research paper-[this paper][1] as i was asked to work on an MRSA bioinfo project, …
Answer: Segmentation error while using a tool by Mensur Dlakic ★ 27k
Most likely you didn't do anything wrong. Going by your previous post that you have only 8 GB of RAM, the most likely reason is the lack of…
Comment: Segmentation error while using a tool by Thanujay S • 0
Hey @Ruqaiya! Can you please give more details on why smalt is opted here? According to what I can see, the tool has become obsolete! Why …
Comment: Extracting mutation status from MAF files - TCGA by Thanujay S • 0
Hey! Can you explain the question further? What have you tried?
Comment: Downloading older version of a tool by Ruqaiya • 0
I tried the 2nd option only. I got the files of older version from the author but that version isn't working on my WSL, it's showing Segmen…
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