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383 results • Page
1 of 8
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0
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0
replies
170
views
Tutorial:
Making Tile plots to show Synonymous Non-Synonymous mutation fraction
mutation
ggplotify
geom_tile
ggplot2
5 days ago by
rohitsatyam102
▴ 780
36
votes
14
replies
32k
views
14 follow
Tutorial:
installing samtools with conda - overcoming two common errors
samtools
conda
updated 23 days ago by
psschlogl
▴ 50 • written 3.3 years ago by
lavinia.gordon
▴ 160
0
votes
0
replies
242
views
Tutorial:
Using easyPubMed and scholar package to get all citations of your paper
scholar
easyPubMed
25 days ago by
rohitsatyam102
▴ 780
0
votes
4
replies
443
views
Tutorial:
Reverse pharmacology: how to predict drugs with genes or disease names?
drugs
drug-targets
genes
19 days ago by
Coremine Medical
▴ 20
90
votes
48
replies
31k
views
29 follow
Tutorial:
Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm
rna-seq
bioconductor
updated 27 days ago by
Ram
41k • written 10.1 years ago by
Irsan
★ 7.8k
48
votes
30
replies
9.3k
views
11 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 4 weeks ago by
ATpoint
78k • written 7.7 years ago by
Ram
41k
1
vote
0
replies
268
views
Tutorial:
How to convert raw counts to TPM for TCGA data and make a heatmap across cancer types
RNAseq
heatmap
rstats
TCGA
4 weeks ago by
Ming Tommy Tang
★ 3.6k
10
votes
31
replies
9.9k
views
10 follow
Tutorial:
OrthoMCL installation on Ubuntu Linux
ubuntu
orthomcl
mysql
linux
updated 5 weeks ago by
ha2606
• 0 • written 5.9 years ago by
vimalkvn
▴ 320
2
votes
0
replies
363
views
Tutorial:
Managing your data (BAM, VCF, sample, phenotype) with RDF and SPARQL.
rdf
sparql
data-management
graph
updated 11 weeks ago by
Ram
41k • written 11 weeks ago by
Pierre Lindenbaum
158k
0
votes
1
reply
314
views
Tutorial:
TAPIS installation and usage
APA
Iso-seq
splicing
TAPIS
analysis
alternative
updated 11 weeks ago by
ATpoint
78k • written 11 weeks ago by
JC
▴ 30
4
votes
2
replies
2.5k
views
Tutorial:
Building mosdepth on macOS
nim
mosdepth
updated 12 weeks ago by
GenoMax
136k • written 3.6 years ago by
ATpoint
78k
0
votes
0
replies
421
views
Tutorial:
multi-omics data integration: a case study with transcriptomics and genomics mutation data
mutation
RNAseq
multiomics
3 months ago by
Ming Tommy Tang
★ 3.6k
0
votes
0
replies
366
views
Tutorial:
T Cell Clonal Analysis Using Single-cell RNA Sequencing and Reference Maps
RNAseq
TCR
single-cell
computational
biology
3 months ago by
Ming Tommy Tang
★ 3.6k
13
votes
3
replies
14k
views
Tutorial:
Plotting the coverage and depth (Y-axis) statistics of a bam file along the genome (X-axis), using Samtools, awk and R.
genome
plot
coverage
samtools
updated 3 months ago by
William
▴ 20 • written 6.7 years ago by
kirannbishwa01
★ 1.6k
5
votes
2
replies
685
views
Tutorial:
Updated List of Unix tutorials 2023
Unix
updated 3 months ago by
Ram
41k • written 3 months ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
316
views
Tutorial:
cellular niches analysis with spatial transcriptome data in Seurat and Bioconductor
transcriptome
rstats
spatial
single-cell
3 months ago by
Ming Tommy Tang
★ 3.6k
9
votes
4
replies
4.5k
views
Tutorial:
Unsupervised clustering on gene expression data
gene-expression
clustering
RNA-seq
3 months ago by
Hamid Ghaedi
3.1k
6
votes
0
replies
355
views
Tutorial:
Polygenic Risk Score Calculation
genetic-burden
r
prs
ggplot
glm
3 months ago by
barslmn
★ 2.0k
0
votes
0
replies
501
views
Tutorial:
How to preprocess and visualize beautifully scRNA-seq with omicverse?
scRNA-seq
seurat
scanpy
Python
3 months ago by
Starlitnightly
• 0
1
vote
0
replies
422
views
Tutorial:
common mistakes in using spreadsheets
excel
spreadsheet
structured-data
updated 4 months ago by
Ram
41k • written 4 months ago by
Ming Tommy Tang
★ 3.6k
3
votes
1
reply
436
views
Tutorial:
Coordinate Systems and genomic variant description
coordinate-system
HGVS
normalization
VCF
updated 4 months ago by
Ram
41k • written 4 months ago by
Juke34
8.4k
84
votes
29
replies
30k
views
20 follow
Tutorial:
How to create a mutation landscape (waterfall) plot with GenVisR
BioConductor
GenVisR
Graphics
R
updated 4 months ago by
amziepickles
• 0 • written 7.7 years ago by
Obi Griffith
20k
1
vote
0
replies
468
views
Tutorial:
How to convert gene ids in R
computational-biology
gene-id
rstats
updated 4 months ago by
Ram
41k • written 4 months ago by
Ming Tommy Tang
★ 3.6k
0
votes
0
replies
646
views
Tutorial:
Differentially expressed gene analysis in Python with omicverse
RNA-seq
Python
4 months ago by
Starlitnightly
• 0
19
votes
13
replies
7.9k
views
10 follow
Tutorial:
Setting up Aspera Connect (ascp) on Linux and macOS
ascp
aspera
updated 28 days ago by
jude
• 0 • written 17 months ago by
ATpoint
78k
2
votes
0
replies
2.9k
views
Tutorial:
How to read multiple matrices from GEO into R and make multiple Seurat objects and merge them into a single object.
unix
Seurat
single-cell
rstats
updated 4 months ago by
Ram
41k • written 4 months ago by
Ming Tommy Tang
★ 3.6k
18
votes
7
replies
6.0k
views
Tutorial:
scRNA-seq CITE-seq-count bioinformatics
CITE-seq-count
scRNA-seq
RNA-Seq
updated 4 months ago by
ATpoint
78k • written 3.7 years ago by
colindaven
5.7k
3
votes
2
replies
658
views
Tutorial:
How to use UCSC genome browser to check the transcription factor binding at your favorite gene
UCSC
transcription
factor
ChIP-seq
4 months ago by
Ming Tommy Tang
★ 3.6k
88
votes
22
replies
83k
views
14 follow
Tutorial:
How to Install SamTools, HTSLib, and BCFTools on Ubuntu 18.04
bcftools
samtools
htslib
updated 4 months ago by
sifa.khan
• 0 • written 5.4 years ago by
otwtgin2010
▴ 580
2
votes
0
replies
334
views
Tutorial:
Understanding Hierarchical Clustering in R, Distance Measures, and Linkage Methods
clustering
gene-expression
rstats
updated 4 months ago by
Ram
41k • written 4 months ago by
Ming Tommy Tang
★ 3.6k
4
votes
0
replies
517
views
Tutorial:
How to do GSEA in R for RNAseq data
gsea
RNAseq
rstats
5 months ago by
Ming Tommy Tang
★ 3.6k
0
votes
0
replies
438
views
Tutorial:
How to read all files into a single dataframe (merge all RNAseq count table)
RNA-seq
rstats
updated 5 months ago by
Ram
41k • written 5 months ago by
Ming Tommy Tang
★ 3.6k
0
votes
0
replies
437
views
Tutorial:
Free Book : Computational Biology of a cell signalling pathway using R
cell-signalling
R
Computational-Biology
5 months ago by
sinha.shriprakash
▴ 20
1
vote
2
replies
629
views
Tutorial:
How to ask computational biology questions
computational-biology
updated 5 months ago by
Ram
41k • written 5 months ago by
Ming Tommy Tang
★ 3.6k
1
vote
0
replies
467
views
Tutorial:
How to pre-process scRNAseq data from fastq to count matrix with two lines of code
unix
scRNA-seq
single-cell
rstats
updated 5 months ago by
Ram
41k • written 5 months ago by
Ming Tommy Tang
★ 3.6k
2
votes
0
replies
518
views
Tutorial:
best practices for single-cell RNAseq analysis
cell
single
scRNAseq
5 months ago by
Ming Tommy Tang
★ 3.6k
1
vote
0
replies
463
views
Tutorial:
How to make a perfect heatmap using RNAseq data
heatmap
RNAseq
rstats
5 months ago by
Ming Tommy Tang
★ 3.6k
0
votes
0
replies
510
views
Tutorial:
How to start disease research quickly and easily?
proteins
pathways
disease
research-design
genes
5 months ago by
Coremine Medical
▴ 20
2
votes
0
replies
518
views
Tutorial:
Get a list of genes associated with a drug in 1 min
drug
genes
drug-targets
updated 5 months ago by
Ram
41k • written 5 months ago by
Coremine Medical
▴ 20
3
votes
0
replies
776
views
Tutorial:
How to make a heatmap in R and deep understand it
heatmap
r
6 months ago by
Ming Tommy Tang
★ 3.6k
35
votes
17
replies
16k
views
12 follow
Tutorial:
Machine Learning For Cancer Classification - Part 3 - Predicting With A Random Forest Classifier
machine-learning
classification
updated 7 months ago by
GenoMax
136k • written 10.0 years ago by
Obi Griffith
20k
2
votes
0
replies
854
views
Tutorial:
ChIP-Seq Analysis Tutorial
Epigenetics
Bowtie2
MACS2
ChIP-Seq
Motif
updated 8 months ago by
ATpoint
78k • written 8 months ago by
Simon
▴ 40
1
vote
0
replies
591
views
Tutorial:
List of Machine Learning-Based Scoring Function Papers
machine-learning
docking
papers
updated 23 days ago by
Ram
41k • written 8 months ago by
Milad
▴ 10
3
votes
2
replies
2.5k
views
Tutorial:
Continuing Education (Pipelines): Best blogs, Online Lectures, Workshops, etc..
conferences
Workshops
updated 6 months ago by
Ram
41k • written 6.6 years ago by
jnowacki
▴ 100
7
votes
3
replies
2.0k
views
Tutorial:
Automated dbSNP lookup by rsID position, plus genome build liftover
rsid
hg19
liftover
hg38
dbsnp
updated 7 months ago by
Randy H
▴ 110 • written 15 months ago by
Kevin Blighe
86k
3
votes
3
replies
2.4k
views
Tutorial:
Generating count matrix for STAR counts in GDC v32.0 for RNA-Seq
GDC
TCGABiolinks
STAR
updated 9 months ago by
Ram
41k • written 19 months ago by
DareDevil
★ 3.3k
2
votes
0
replies
934
views
Tutorial:
Analysis of Smart-Seq3 data with kallisto-bustools
single-cell
kallisto
transcriptomics
smart-seq3
pipeline
updated 16 days ago by
Ali
• 0 • written 10 months ago by
firestar
★ 1.6k
11
votes
7
replies
1.9k
views
Tutorial:
Sequence Alignment from Scratch with BWA
alignment
bwa
DNA
8 months ago by
barslmn
★ 2.0k
2
votes
1
reply
1.9k
views
Tutorial:
Converting TSV file to VCF
vcf
preprocessing
frequency
updated 10 months ago by
Pierre Lindenbaum
158k • written 10 months ago by
barslmn
★ 2.0k
4
votes
2
replies
1.9k
views
Tutorial:
Prediction of Ribosomal RNA Genes Using RNAmmer Software
Annotation
rRNA
RNAmmer
Genome
4 months ago by
Dr.Animo
▴ 80
383 results • Page
1 of 8
Recent Votes
A: How to add biological annotations using R
Answer: Mapping quality in `vg giraffe`
Answer: Mapping quality in `vg giraffe`
Answer: Comparing 3 Data Sets using DeSeq and Heatmaps
Comment: Harmony integration PC variance explained
Answer: Is bisulfite-seq data from different studies comparable with each other?
Answer: BULK mRNA-seq with UMIs. Do I need to normalize by gene length?
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Recent Replies
Comment: Where is the index command?
by
Michael
53k
samtools index (like genral programs you install on your machine) should run without sudo, if it doesn't, you might want to check the acces…
Comment: Where is the index command?
by
Michael
53k
I didn't know you were using WSL. Possibly the damage you can do with that is limited to the WSL environment and the worst thing that can h…
Comment: Is bisulfite-seq data from different studies comparable with each other?
by
Basti
★ 1.8k
There is no general rule, what you can apply depends on the nature of each dataset you intend to integrate (tissue, condition, technique, s…
Comment: Where is the index command?
by
Michael
53k
Running this is always a good idea.
Answer: Salmon indices differences
by
ATpoint
78k
In theory the full genome decoy is the best since it includes the entire genome, hence decoying potential spurious mappings due to DNA cont…
Comment: Can you help me to download list of miRNA from a SRA under a bioproject ?
by
Nitin Narwade
★ 1.5k
You can directly ask authors but as it is still on bioRxiv I don't think they can share this data with you until it get published somewhere…
Comment: Can you help me to download list of miRNA from a SRA under a bioproject ?
by
abourrieau
• 0
Thank you, I had thought about this possibility but I don't see where it is indicated on the NCBI website: : https://www.ncbi.nlm.nih.gov/b…
Comment: Cellcyclescoring did not work in Seurat V5
by
fracarb8
★ 1.3k
You can try to merge the layer together with `data.filt <- JoinLayers(data.filt)`
Comment: Mapping quality in `vg giraffe`
by
Wenhai
• 0
Many thanks.
Comment: Can you help me to download list of miRNA from a SRA under a bioproject ?
by
Nitin Narwade
★ 1.5k
It seems the data for this BioProject is not publicly available yet. It is a good practice to check first on the NCBI website. Regards, N…
Comment: How to check for incorporation of plasmid DNA into human genomic DNA (ChIP-seq)
by
Nitin Narwade
★ 1.5k
Hi, Nobody has answered you yet, probably because there are very basic things you assumed wrong, so I will try to comment on this. The f…
Comment: calculate nucleotide diversity from whole-genome-sequence data for individual ge
by
KL_STKLBK
• 0
This is not really answering the question - one can loop say VCFtools, but it would still be windows/single site based. DnaSP also does not…
Comment: Is bisulfite-seq data from different studies comparable with each other?
by
CTLong
▴ 20
Hi Colin, thanks for the reply. What about integration of RNA-seq expression from different studies? I guess this is more widely accepted r…
Comment: calculate nucleotide diversity from whole-genome-sequence data for individual ge
by
J
• 0
Thanks, but I still need a method that actually calculates per gene nucleotide diversity. And as mentioned DnaSP6 does not give the option …
Comment: R script in docker
by
Jean-Karim Heriche
26k
This isn't a bioinformatics question. To access data that sits outside the container from within the container, you need to make it accessi…
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