updated 3 days ago by
cwwong13
▴
40
•
written 6.7 years ago by
lessismore
★
1.3k
40
votes
10
replies
42k
views
8 follow
28
votes
28
replies
34k
views
11 follow
updated 3 hours ago by
thomas.heigl.ibk
•
0
•
written 12.8 years ago by
Bioscientist
★
1.7k
19
votes
11
replies
14k
views
10 follow
10
votes
6
replies
9.9k
views
6 follow
updated 6 days ago by
Antonio R. Franco
★
5.1k
•
written 6.0 years ago by
williamsbrian5064
▴
510
3
votes
8
replies
6.4k
views
7 follow
updated 2 hours ago by
Sd
•
0
•
written 5.1 years ago by
Nicolas Rosewick
11k
4
votes
6
replies
3.8k
views
updated 2 days ago by
Andres
▴
20
•
written 9.6 years ago by
hermathena
▴
40
1
vote
6
replies
2.8k
views
updated 3 days ago by
dimpleadiwal050896
•
0
•
written 4.9 years ago by
ggman
▴
90
2
votes
4
replies
2.6k
views
0
votes
9
replies
2.5k
views
6 follow
1
vote
14
replies
2.2k
views
updated 17 minutes ago by
GenoMax
142k
•
written 4.5 years ago by
Swimming bird
▴
20
4
votes
9
replies
2.1k
views
3 days ago by
anna
▴
20
3
votes
6
replies
2.0k
views
7 follow
38
votes
24
replies
1.9k
views
10 follow
2
votes
7
replies
1.3k
views
6 follow
updated 3 days ago by
Gordon Smyth
★
7.1k
•
written 8 months ago by
Calum
▴
10
1
vote
4
replies
1.0k
views
8
votes
16
replies
934
views
2
votes
5
replies
924
views
2
votes
11
replies
889
views
updated 3 days ago by
jkbonfield
★
1.2k
•
written 15 days ago by
me
•
0
0
votes
1
reply
746
views
0
votes
1
reply
746
views
0
votes
3
replies
726
views
5
votes
16
replies
681
views
3 days ago by
Ruqaiya
•
0
4
votes
7
replies
677
views
7 follow
updated 5 days ago by
dsull
★
5.9k
•
written 9 days ago by
qudrat.nii
▴
10
4
votes
8
replies
634
views
4
votes
7
replies
570
views
3 days ago by
e.r.zakiev
▴
210
0
votes
11
replies
567
views
0
votes
0
replies
565
views
0
votes
1
reply
548
views
1
vote
7
replies
484
views
updated 3 days ago by
bk11
★
2.4k
•
written 7 days ago by
starswillfade
▴
10
0
votes
10
replies
482
views
5
votes
6
replies
477
views
2
votes
5
replies
446
views
4 days ago by
ashaneev07
▴
20
1
vote
8
replies
439
views
2 days ago by
marco.barr
▴
90
4
votes
4
replies
436
views
updated 5 days ago by
BioinfGuru
★
1.7k
•
written 7 days ago by
Ming Tommy Tang
★
3.9k
0
votes
1
reply
428
views
0
votes
1
reply
423
views
updated 1 day ago by
Ram
43k
•
written 1 day ago by
Dr Huma Naz
•
0
0
votes
8
replies
418
views
0
votes
6
replies
418
views
22 hours ago by
kl
▴
10
9
votes
10
replies
397
views
6 follow
updated 5 hours ago by
Zamin Iqbal
▴
20
•
written 1 day ago by
nicole.kavanagh
•
0
0
votes
5
replies
396
views
4
votes
2
replies
394
views
updated 6 days ago by
shalespringer
•
0
•
written 12 weeks ago by
Phenylananin
▴
20
1
vote
3
replies
379
views
updated 4 days ago by
Mensur Dlakic
★
27k
•
written 5 days ago by
O.rka
▴
710
0
votes
3
replies
377
views
2 days ago by
Assa Yeroslaviz
★
1.8k
1
vote
4
replies
377
views
3 days ago by
hagl
▴
10
1
vote
3
replies
370
views
3
votes
2
replies
359
views
0
votes
5
replies
357
views
updated 5 days ago by
Istvan Albert
100k
•
written 8 days ago by
sehriban.buyukkilic
▴
10
4
votes
3
replies
355
views
updated 5 days ago by
Arup Ghosh
3.2k
•
written 6 days ago by
chaco001
▴
40
2
votes
3
replies
349
views
updated 6 days ago by
dsull
★
5.9k
•
written 23 days ago by
javanokendo
▴
60
Recent Votes
Recent Locations •
All
United States, just now
Mexico, 1 minute ago
United States, 1 minute ago
Stony Brook University, NY, 3 minutes ago
United States, 5 minutes ago
United Kingdom, 5 minutes ago
Switzerland, 6 minutes ago
Recent Awards •
All
Popular Question to
yvonnehhe
•
0
Popular Question to
curious
▴
750
Scholar to
jared.andrews07
★
16k
Popular Question to
hafiz.talhamalik
▴
350
Popular Question to
Hayler Edu
▴
40
Popular Question to
eesiribloom
▴
80
Popular Question to
g.persic1991
•
0
Recent Replies
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
If you know what the boundaries of your construct look like then trim the left-end of the read using the tag on that end (`ktrim=l`). Then …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> Not every read in your data is going to match a guide.
That is also true for sure. Let me clarify what I mean. For every read you should…
Answer: VG : No reference-sense paths available in the graph; falling back to generic pa
by
anovak
▴
120
If your GFA has paths in it that are P lines with names that don't include a sample name, contig name, and separators, then those are what …
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
> if you look at a fastq file you should be able to tell for every read definitely, if it has a perfect match for a guide in it and what gu…
Answer: DiffBind: no peaks in DBA
by
jared.andrews07
★
16k
Because `dba.analyze` is not meant to be run directly on a peaks file. Have you read the documentation?
In addition, a ChIP-seq experiment…
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as yo…
Comment: Converting CRAM to FastQ
by
GenoMax
142k
Use `samtools view ` for this conversion. See discussion in https://www.biostars.org/p/9592860/
Specifically @jkbonfield's comment here --…
Comment: How to access TCGA samples that were treated with a specific drug?
by
GenoMax
142k
No that was only from Breast cancer. You could try selecting all data and see if you are able to see all treatments in the set.
No idea a…
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as you …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴
80
> Can you try lowering the k to k=9?
Wouldn't that mess with the results since all my kmers are 20bp?
> You don't have any duplicate sequ…
Comment: Provean help
by
Mensur Dlakic
★
27k
> Else, I will happy to send over the data and kindly perform the analysis for me.
This website is meant to provide advice, not service. I…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴
40
Thank you! Naive question, but does that list include all treatments in TCGA? I.e. is there anything missing from there, or that's it?
Als…
Comment: Base Quality Score Recalibration with different reference genome
by
dtnondorf
•
0
Interesting! Thank you for such a quick response!
Comment: GATK genomicsDBimport intervals for WGS
by
Sd
•
0
I also have similar question. I sliced the genomic bed file with 50kb windows and 1kb padding into ~700 bed files; each bed file contains 9…
Traffic: 2651 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6